Protoplasts were digested from embryogenic calli of type Ⅱ that were friable, no—nucilaginous and repidly growing. Plating frequency of 5.6%—7.4% was obtained when protoplasts were cultured in KPR or Neap medium by thin liquid layer culture method at density of 1×10~5 cells/ml.
Mesophyll protoplasts of the tobacco plant (Nicotiana tabacum) were releasedby EA_3-867 cellulase and cultivated in NT medium (Nagata and Takebe 1971)with thin liquid layer method. The respiratory inhibitor, NaF, of Embden-Me-yerhor-Parnas pathway (EMP) or the inhibitor, Na_3PO_4, of Hexose Monophatepathway (HMP) was added into the basal medium. Then calcoflur white VBLwas used to examine their effects on the formation of the cell wall.
and cultured in modified Miller medium. The high frequency division of the regenerated cells was observed,It was indicated that the agarose bead culture with B_6S_3 nurse cells is the most suitable for the cotyledon protoplast of Xinjiang muskmelon when compared with thin liquid culture and double layer culture.
的无菌苗子叶游离原生质体,用改良的 Miller 培养基(Ma)培养,得到了再生细胞的高频率分裂。 比较了液体浅层培养、双层培养与琼脂糖珠看护培养等方法,发现由烟草瘤细胞 B_6S_3看护的琼脂培珠培养,最宜于新疆甜瓜子叶的原生质体。
The yield of purified protoplasts isolated from hypocotyl of 6 d old Brassica oleracea seeding was 1.45Z×106g-1 FW. The protoplasts were cultured in KM 8 p liquid medium supplemented with 1.5mg/L 6-BA, 0.5mg/L NAA and 0.5mg/L 2,4-D.
Liquid culture method was appropriate for the protoplast division. The highest division frequency was 38% in DPD medium supplemented with 2.0 mg/L 2,4-D, 0.2mg/L 6-BA, 0.3M mannitol, 2%(w/v) sucrose and 500mg/L casein hydroIysate(CH) at a plating density of 3×105/ml.
Foam and wetting films are convenient models for the study of symmetric (free thin liquid films) and asymmetric (thin liquid films on solid substrate) films with the same air/solution interface.
The CTAB concentration ranges, which provide the formation of unstable (rupturing and metastable) and stable films, are different for symmetric (foam) and asymmetric (wetting) thin liquid films.
Nonlinear Phenomena in Evaporating Thin Liquid Film Flow
The position of the lines of separation is determined experimentally from the confluence of thin liquid films applied to the surface.
A more detailed derivation of quasi-one-dimensional asymptotic equations for the dynamics of thin liquid jets and an analysis on the basis of them of the curved decay shape of the jet in the linear and nonlinear stages is contained in [1, 2].