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大鼠原位部分缺血
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  partial liver ischemia
     Fifty-four SD rats were randomly divided into ischemic preconditioning group (IP), ischemia/Reperfusion group (IR) and sham operation group (SO). The model of partial liver ischemia/reperfusion was used. The specimen was collected at 0, 1, 2, 4 h after ischemia/reperfusion whether there was ischemic preconditioning or not.
     方法采用大鼠原位部分缺血再灌注模型,54只SD大鼠随机分为缺血再灌注组(IR),缺血预处理组(IP)与假手术组(SO组),应用RT-PCR法检测各组复灌后0,1,2,4 h肝组织CyclinD1 mRNA的变化。
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  相似匹配句对
     Auxiliary partial orthotopic liver transplantation in rats
     大鼠原位辅助性部分肝移植
短句来源
     Methods: The models of sham-operation and partial hepatic ischemia were established in rats.
     方法 :建立大鼠部分肝脏热缺血再灌注模型。
短句来源
     A MODEL OF ORTHOTOPIC PARTIAL LIVER TRANSPLANTATION IN RATS AND ITS TECHNICAL MODIFICATION
     大鼠原位部分肝移植模型的建立及技术改进
短句来源
     2. We use the rat model of warm ischemia and reperfusion in situ in single lung.
     2.采用大鼠单肺原位缺血再灌注模型。
短句来源
     EFFECTS OF ESTROGEN ON REDUCED-SIZE LIVER ISCHEMIA REPERFUSION INJURY IN RATS
     雌激素对大鼠部分缺血再灌注损伤的影响
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  partial liver ischemia
The results indicated that TLR2 mRNA in ischemic lobe was up-regulated markedly in mice partial liver ischemia/reperfusion injury model compared to that in sham operation group (ΔCt: 1.05±1.02 vs 5.08±1.36,P>amp;lt;0.001).
      
In the HS group, rats underwent whole-body hyperthermia followed by 60-min partial liver ischemia.
      
The hepatoprotective effects of misoprostol, a PGE1 analog, against ischemiareperfusion liver injury were studied using a rat partial liver ischemia model.
      


To observe the effect of ischemic preconditioning on the cyclinD1 mRNA expression of rats liver cell during early ischemic reperfusion. Fifty-four SD rats were randomly divided into ischemic preconditioning group (IP), ischemia/Reperfusion group (IR) and sham operation group (SO). The model of partial liver ischemia/reperfusion was used. The specimen was collected at 0, 1, 2, 4 h after ischemia/reperfusion whether there was ischemic preconditioning or not. In each group, the cyclinD1 mRNA was detected by RT-PCR....

To observe the effect of ischemic preconditioning on the cyclinD1 mRNA expression of rats liver cell during early ischemic reperfusion. Fifty-four SD rats were randomly divided into ischemic preconditioning group (IP), ischemia/Reperfusion group (IR) and sham operation group (SO). The model of partial liver ischemia/reperfusion was used. The specimen was collected at 0, 1, 2, 4 h after ischemia/reperfusion whether there was ischemic preconditioning or not. In each group, the cyclinD1 mRNA was detected by RT-PCR. Compared with IR group, the cyclinD1 mRNA expression was significantly different at early reperfusion (0, 1 h), in IP group. (0.568±0.112 vs 0.274±0.069, 0.762±0.164 vs 0.348±0.093, P<0.01). [Conclusion] Ischemie preconditioning before hepatic ischemia/reperfusion can promote the cyclinD1 mRNA expression in the early reperfusion.

目的观察缺血预处理对大鼠肝缺血再灌注损伤早期细胞CyclinD1 mRNA表达变化的影响。方法采用大鼠原位部分缺血再灌注模型,54只SD大鼠随机分为缺血再灌注组(IR),缺血预处理组(IP)与假手术组(SO组),应用RT-PCR法检测各组复灌后0,1,2,4 h肝组织CyclinD1 mRNA的变化。结果与IR组相比,IP组在复灌早期(0,1 h),肝组织的CyclinD1 mRNA表达明显增高。(0.568±0.112 vs 0.274±0.069,0.762±0.164 vs 0.348±0.093,P<0.01)。结论缺血预处理可促进肝细胞在缺血再灌注损伤后早期Cy-clinD mRNA的表达。

To investigate the effects of preconditioning on expression of immediate early genes c-fos and c-jun following hepatic ischemia/reperfusion (IR) and its roles in cellular regeneration and apoptosis. Ninety-six Wistar rats were randomly divided into IR group and IPC group, each group was divided into eight sub-groups (n =6). The model of partial liver ischemia/reperfusion was used. Rats subjected to 60 min liver ischemia, preceded by 10 min preconditioning. After 0, 0.5, 1, 2, 4, 8, 12 and 24 h reperfusion,...

To investigate the effects of preconditioning on expression of immediate early genes c-fos and c-jun following hepatic ischemia/reperfusion (IR) and its roles in cellular regeneration and apoptosis. Ninety-six Wistar rats were randomly divided into IR group and IPC group, each group was divided into eight sub-groups (n =6). The model of partial liver ischemia/reperfusion was used. Rats subjected to 60 min liver ischemia, preceded by 10 min preconditioning. After 0, 0.5, 1, 2, 4, 8, 12 and 24 h reperfusion, the serum and liver tissue in each group were collected to detect the serum ALT/AST , liver histopathology, expression of c-fos and c-jun mRNA. Flow cytometer was used to detect Ki67 and Sub-G1 as the quantity indicators of cell regeneration and apotosis respectively. Compared with group IR, group IPC showed significantly lower ALT/AST level in sub-group 0.5 h to sub-group 8 h (P <0.05); Ki67 elevated significantly at 0.5, 1, 2 h, but decreased significantly at 24 h (P <0.05); Ap index decreased significantly after 1h reperfusion(P <0.05); Expression of c-fos and c-jun mRNA were low, especially c-jun at 0.5, 1 and 2 h after reperfusion. [Conclusion] Ischemic preconditioning can protect liver cells against ischemia/reperfusion injury, this protective effect may be related to influence transcription levels of c-fos and c-jun.

目的观察缺血预处理对大鼠肝缺血再灌注后即早基因c-fos、c-jun表达的影响。方法采用大鼠原位部分缺血再灌注模型,96只SD大鼠随机分为缺血再灌注组(IR),缺血预处理组(IPC),每组又分为8个亚组(n=6),于复灌后0、0.5、1、2、4、8、12和24h取材,应用RT-PCR法检测各组c-fos、c-junmRNA的表达,流式细胞仪检测Ki67和Sub-G1。结果与IR组相比,IPC组血清ALT、AST在复灌后的0.5 ̄8h组明显降低(P<0.05);Ki67在复灌后的0.5、1和2h明显升高,24h明显降低(P<0.05);Ap指数在复灌后的1h以上明显降低(P<0.05);IPC组c-fos和c-junmRNA的表达较IR组低,其中c-jun在0.5、1和2h组明显降低(P<0.05)。结论缺血预处理能有效地保护肝脏免受缺血再灌注造成的损伤,这种保护效应的机制可能与影响即早基因的转录有关。

 
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