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反向线点
相关语句
  reverse line blot
    Simultaneous Detection of Four Common Pathogenic Mycoplasma by Reverse Line Blot (RLB) Hybridization
    反向线点杂交方法检测和鉴定4种常见致病支原体
短句来源
    Simultaneous detection and genotyping of Chlamydia trachomatis using polymerase China reaction and reverse line blot hybridization assay
    聚合酶链反应与反向线点杂交检测沙眼衣原体及分型
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  “反向线点”译为未确定词的双语例句
    Methods Compare 198 clinic specimen results detected by RLB and mycoplasmas specific species PCR.
    方法应用支原体反向线点杂交方法和种特异性PCR方法同时检测198例临床标本,比较两种方法的检测结果。
短句来源
    Results Mycoplasmas positive rate was 33.3% and 34.3%, respectively in 198 clinic specimens.
    结果支原体反向线点杂交方法和种特异性PCR检测支原体阳性率分别为33.3%和34.3%,两种方法检测结果符合率为98.5%。
短句来源
    Conclusion The RLB hybridization assay is sensitive and specific, and able to rapidly detect and identify mycoplasmas species from clinical specimens.
    结论反向线点杂交方法能同时快速、敏感和特异的检测这4种支原体。
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  reverse line blot
Zur Identifizierung der Genospezies wurden zwei Methoden eingesetzt, die RFLP (restriction fragment length polymorphism) und der "reverse line blot".
      
For this purpose the infection rate of partners from tick couples was determined by polymerase chain reaction and reverse line blot.
      
HPV testing was conducted with the Hybrid Capture II (HC II) system and polymerase chain reaction (PCR) with genotyping using the reverse line blot method.
      
garinii and group VS116 were identified by reverse line blot (RLB) using genomospecies specific oligonucleotide probes.
      
Comparison between reverse line blot probing and RFLP methods to detect Theileria annulata and Theileria Lestoquardi in livestock animals and ticks.
      
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Objective Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, Ureaplasma urealyticum were detected and identified by reverse line blot hybridization (RLB) to evaluate the sensitity of the assay.Methods Compare 198 clinic specimen results detected by RLB and mycoplasmas specific species PCR.Results Mycoplasmas positive rate was 33.3% and 34.3%, respectively in 198 clinic specimens. 195 of 198 specimens gave concordant results. Only three specimens including Ureaplasma parvum were detected by specific...

Objective Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, Ureaplasma urealyticum were detected and identified by reverse line blot hybridization (RLB) to evaluate the sensitity of the assay.Methods Compare 198 clinic specimen results detected by RLB and mycoplasmas specific species PCR.Results Mycoplasmas positive rate was 33.3% and 34.3%, respectively in 198 clinic specimens. 195 of 198 specimens gave concordant results. Only three specimens including Ureaplasma parvum were detected by specific species PCR, but not by RLB. Detection results were not different using RLB and specific species PCR. Conclusion The RLB hybridization assay is sensitive and specific, and able to rapidly detect and identify mycoplasmas species from clinical specimens.

目的评价反向线点杂交方法检测生殖支原体、人型支原体、微小脲原体和解脲脲原体的敏感性。方法应用支原体反向线点杂交方法和种特异性PCR方法同时检测198例临床标本,比较两种方法的检测结果。结果支原体反向线点杂交方法和种特异性PCR检测支原体阳性率分别为33.3%和34.3%,两种方法检测结果符合率为98.5%。3例标本支原体种特异性PCR方法检测微小脲原体阳性,而反向线点杂交方法检测为阴性。两种方法检测支原体结果无显著性差异(P>0.05)。结论反向线点杂交方法能同时快速、敏感和特异的检测这4种支原体。

Objective To develope and evaluate a new multiple and nested PCR-reverse line blot (RLB) hybridization assay for the detection of Chlamydia trachomatis infections and typing.Methods Two sets of primers directed to the VD2 region of the omp1 gene of C.trachomatis and one set of primers directed to the cryptic plasmid of C.trachomatis were designed. The assay was developed for the detection and typing of 15 different serovars of C.trachomatis(A?B/Ba?C?D/Da?E?F?G/Ga?H?I/Ia?J?Ja? K?L1?L2 and L3). The assay developed...

Objective To develope and evaluate a new multiple and nested PCR-reverse line blot (RLB) hybridization assay for the detection of Chlamydia trachomatis infections and typing.Methods Two sets of primers directed to the VD2 region of the omp1 gene of C.trachomatis and one set of primers directed to the cryptic plasmid of C.trachomatis were designed. The assay was developed for the detection and typing of 15 different serovars of C.trachomatis(A?B/Ba?C?D/Da?E?F?G/Ga?H?I/Ia?J?Ja? K?L1?L2 and L3). The assay developed was tested with 277 urine ,rectal swab of 2 men and cervical scrapes of 78 women previously for C. trachomatis by using COBAS Amplicor test.Results 175 of 192 COBAS Amplicor-positive specimens were positive by PCR- RLB.Using this assay, we were able to type 163 of the 175 samples (93.1%).Six of 163 COBAS Amplicor -negative specimens were positive by nested PCR- RLB.Of these samples, 85.3% were single C. trachomatis infections, and 14.7% were multiple infections. The most common serovars identified were serovars E(38.6%),F(28.5%), D (18.2%).Of the people with multiple C.trachomatis infections contained main both serovars H and K.Conclusions The multiple PCR-RLB combine with nested PCR developed is a simple, fast, sensitive and specific method for the detection and subtype of C. trachomatis.Moreover, it is an appropriate method for studying multiple C. trachomatis infections and for use in large epidemiological studies.

目的建立和评价一个新的多重和巢式PCR反向线点杂交试验(RLB)检测泌尿生殖道沙眼衣原体及其分型方法。方法该试验设计了3对引物分别针对CT外膜蛋白(omp1)基因VD2区和质粒进行扩增,用CT15种血清型(A、B/Ba、C、D/Da、E、F、G/Ga、H、I/Ia、J、Ja、K、L1、L2和L3)探针与扩增后的产物杂交。经COBASAmplicor检测的355份标本,其中277份尿液,2份男性直肠拭子和78份女性宫颈试子,进行PCRRLB检测CT并分型。结果192份COBASAmplicor阳性标本中175份PCRRLB阳性,其中93.1%(163/175)的CT感染可被正确分型,其单一型感染分型结果与DNA测序结果一致。163份COBASAmplicor阴性标本中6份巢式PCRRLB阳性。85.3%(139/163)为单一型CT感染,最常见的CT是E(38.6%)F(28.5%)和D(18.2%)。14.7%(24/163)为CT混合感染,混合感染以H和K为主。结论PCRRLB检测CT是一种简便、快速、特异和敏感的方法,并准确的进行CT分型,为CT感染的临床诊断和分子流行病学研究提供了一种可靠的...

目的建立和评价一个新的多重和巢式PCR反向线点杂交试验(RLB)检测泌尿生殖道沙眼衣原体及其分型方法。方法该试验设计了3对引物分别针对CT外膜蛋白(omp1)基因VD2区和质粒进行扩增,用CT15种血清型(A、B/Ba、C、D/Da、E、F、G/Ga、H、I/Ia、J、Ja、K、L1、L2和L3)探针与扩增后的产物杂交。经COBASAmplicor检测的355份标本,其中277份尿液,2份男性直肠拭子和78份女性宫颈试子,进行PCRRLB检测CT并分型。结果192份COBASAmplicor阳性标本中175份PCRRLB阳性,其中93.1%(163/175)的CT感染可被正确分型,其单一型感染分型结果与DNA测序结果一致。163份COBASAmplicor阴性标本中6份巢式PCRRLB阳性。85.3%(139/163)为单一型CT感染,最常见的CT是E(38.6%)F(28.5%)和D(18.2%)。14.7%(24/163)为CT混合感染,混合感染以H和K为主。结论PCRRLB检测CT是一种简便、快速、特异和敏感的方法,并准确的进行CT分型,为CT感染的临床诊断和分子流行病学研究提供了一种可靠的方法。

 
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