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   特异性杂交 的翻译结果: 查询用时:0.455秒
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特异性杂交
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  specific hybridization
     r-32P-ATP 5'-end-labelled probes showed specific hybridization bands by Southern blot analysis of CCK-AR and CCK-BR mRNA products.
     用γ-32P—ATP标记的两种特异性探针分别对CCK—AR和CCK—BR mRNA RT—PCR扩增产物进行Southern分子杂交,结果均有特异性杂交带出现。
短句来源
     Its efficiency for specific hybridization was 95%.
     探针特异性杂交效率达95%。
短句来源
     Results:The 16S rRNA and 23S rRNA gene fragments of 16 species of pathogenic bacteria were amplified with multi-PCR at the same condition. The corresponding specific hybridization maps were obtained applying oligonucleotide microarray technology to detect and identify Enterobacteriaceae foodborne infection bacteria.
     结果:在同一条件下运用多重PCR扩增出14种(属)细菌的16S rRNA、23S rRNA基因片段,随后应用寡核苷酸芯片技术对致病菌进行检测得到了相应的特异性杂交图谱。
短句来源
     The extracted RNA from FMDV infected cells were reverse transcripted, followed by PCR amplification and labeling with fluorescent dyes Cy3. The labeled PCR products were subjected to specific hybridization and subsequently photoscanned using optimal light intensity.
     分别提取5株口蹄疫病毒的RNA,经反转录和PCR掺入Cy3-dCTP荧光标记后,与芯片上的探针特异性杂交,然后对芯片进行扫描,根据荧光强度判断检测结果。
短句来源
     Dynamic allele specific hybridization (DASH), a new method for rapid, economical, accurate, repeatable and high throughput genotyping of single nucleotide polymorphisms (SNP), has been used successfully for genotyping 2 SNPs in 96 samples.
     动态等位基因特异性杂交 (dynamicallele specifichybridization ,DASH)是新发展起来的一种单核苷酸多态(singlenucleotidepolymorphisms,SNP)等位基因分型技术 ,具有快速、经济、准确、高通量、重复性好等优点 .
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  “特异性杂交”译为未确定词的双语例句
     abeled-PCR products of PrV hybridized with capture probes of PRRSV, JEV, PCV2 and PPV, nor did those of PPV and PCV when hybridized with PrV capture probes.
     PrV PCR产物和PRRSV,JEV,PCV2,PPV的捕获探针间无非特异性杂交信号。
短句来源
     The plasmid pRC 102 canbe used to type HSV by DNA hybridization.
     两组实验结果显示,pRC102质粒DNA只与HSV—2 DNA特异性杂交,其HSV—2的型特异性良好。
短句来源
     Peptide nucleic acid(PNA) is a DNA mimic in which the nucleobases are attached to a pseudopeptide backbone.
     肽核酸(peptide nucleic acid, P N A)是 D N A 类似物,具有由氨乙基甘氨酸单位组成的假肽骨架,能以高亲和性和高生物稳定性与 D N A 和 R N A 特异性杂交
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     The special hybridization positive signals appeared in the cells of generation 1 to generation 7 after infection. HCV RNA positive signals were shown as blue purple small graunles in the cytoplasm and on the membrane.
     在感染后的第 1至第 7代细胞内出现特异性杂交信号 ,HCV RNA阳性物呈蓝紫色细小颗粒分布在细胞浆和细胞核内。
短句来源
     ②Western-blot result revealed that there was a clear BMP-9 special hybridism blot at Mr 13 000 of protein in the normal control group, while there was weakly or no expression of BMP-9 gene of liver of rats at days 5, 10 and 20. CONCLUSION: The BMP-9 gene transcription and protein levels in liver of type 2 diabetic rats are significantly lower.
     ②Western-blot结果显示正常组提取的蛋白在Mr13000处可见一条清晰的骨形成蛋白9特异性杂交带,而在5,10,20d处死大鼠肝中骨形成蛋白9表达明显减弱或几乎未见表达。
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  相似匹配句对
     Its efficiency for specific hybridization was 95%.
     探针特异性杂交效率达95%。
短句来源
     Construction of the Keratin and Peroxidase Bispecific Hybrid Hybridoma
     角蛋白和过氧化物酶双特异性杂交杂交瘤的建立
短句来源
     The specificity was also 100%.
     特异性100%。
短句来源
     northern hybridization.
     Northern杂交
短句来源
     e-Hybrid Rice
     e杂交
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  specific hybridization
Analysis of mixed DNA from several individuals of each line revealed specific hybridization bands that could serve as DNA markers during selection for the high and low corticosterone levels in blood.
      
Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles.
      
thermocellum polymerase chain reaction products that represented different genes and found gene specific hybridization in each case.
      
Specific hybridization to secretin mRNA was observed in low abundance in many germ cell types, but was heaviest over step 19 spermatids in stages VII and VIII tubules.
      
The gene repair approach relies on specific hybridization of the oligonucleotides to the target gene generating a mismatch with the targeted point mutation.
      
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The DNA of Herpes Simplex Virus type 2 (HSV-2)digested with restrictionenzyme BamHI and the BamHI G fragment was isolated, which locates in thereverse repeat sequence and spans the junction site of L and S component.Then the BamHI G was inserted on the BamHI cut-site of the vector plasmidpUC 8. In order to modify this recombinant plasmid, the EcoRI--KPN I fra-gment of the recombinant plasmid was removed by restriction enzyme digestionwith EcoRI and KPN 1. After modification of the ends and ligation of them,a...

The DNA of Herpes Simplex Virus type 2 (HSV-2)digested with restrictionenzyme BamHI and the BamHI G fragment was isolated, which locates in thereverse repeat sequence and spans the junction site of L and S component.Then the BamHI G was inserted on the BamHI cut-site of the vector plasmidpUC 8. In order to modify this recombinant plasmid, the EcoRI--KPN I fra-gment of the recombinant plasmid was removed by restriction enzyme digestionwith EcoRI and KPN 1. After modification of the ends and ligation of them,a new recombinant plasmid pRC 102 was constructed which still contains asmall HSV-2 DNA fragment. Labeled with ~(32)P, the recombinant plasmid pRC102 was utilized to hybridize with HSV-1, HSV-2 DNA and other DNA. Theresults of the dot blot and the Southern blot hybridization showed that the probepRC 102 DNA hybridized only with HSV-2 DNA. The plasmid pRC 102 canbe used to type HSV by DNA hybridization.

用核酸限制性内切酶BamHI对单纯疱疹病毒2型(HSV—2)的DNA进行酶解,回收位于基因组中的反向重复序列区的Bam HIG片段,然后将其克隆在载体质粒PUC 8的Bam HI切点上,进一步用核酸限制性内切酶Eco RI和KPNI对这一重组质粒联合酶解,移去EcoRI—KPNI小片段,经末端修饰后,将其连接得到新的重组质粒pRC102,它含有一小段HSV—2的DNA序列。以此质粒为探针,分别与HSV—1、HSV—2及细胞DNA进行斑点杂交;与HSV—1和HSV—2酶解后的DNA片段进行Southern转印系交。两组实验结果显示,pRC102质粒DNA只与HSV—2 DNA特异性杂交,其HSV—2的型特异性良好。

BALB/c mice were immunized with embryonic chick neural retina.After fusion hybridoma colonies were formed in the top layer of soft agarose culture medium. Milipore filters with cultured neural retina cells or isolated chick brain synaptosomes were placed onto the top of the agarose plates. During the incubation period monoclonal antibody molecules secreted py the hybridoma colonies bind specifically to the antigens on the filter. The filters were then incubated in the buffer containing 125I labeled goat anti-mouse...

BALB/c mice were immunized with embryonic chick neural retina.After fusion hybridoma colonies were formed in the top layer of soft agarose culture medium. Milipore filters with cultured neural retina cells or isolated chick brain synaptosomes were placed onto the top of the agarose plates. During the incubation period monoclonal antibody molecules secreted py the hybridoma colonies bind specifically to the antigens on the filter. The filters were then incubated in the buffer containing 125I labeled goat anti-mouse IgG, IgA and IgM.After autoradiography the position of positive hybridoma colonies were indicated by the presence of dark spots on the X-ray film. This method is suitable for the generation of large number of specific hybridoma clones. Monoclonal antibodies reacting with different cellular or synaptical layers of chick neural retina has been produced with this method.

细胞融合后,在软琼脂糖培养基上形成杂交瘤克隆,如将附有细胞或其他抗原的滤膜置于培养基表面,杂交瘤细胞分泌的抗体通过扩散与滤膜上的抗原结合,经与~(125)Ⅰ标记的第二抗体反应后,将滤膜进行放射自显影以显示阳性克隆的位置。用本法能快速大规模筛选特异性杂交瘤克隆,已用此法得到多株针对鸡胎视网膜的单克隆抗体。

The 2.8 kb fragment related to Sereny test was separated and purified from virulent large plasmid of Y.enterolitica(serotype O:8)and used as a DNA probe which was labeled with hapten digoxigenin.The result of colony hybridization demonstrated that 2.8 kb probe reacted with strains of Y.enterolitica (different serotypes)and Y.pseudotuberculosis which expressed VW antigen,but it did not react with S.sonni,EIEC and E.coli.The above-mentioned results suggested that 2.8 kb probe have high specificity and be not related...

The 2.8 kb fragment related to Sereny test was separated and purified from virulent large plasmid of Y.enterolitica(serotype O:8)and used as a DNA probe which was labeled with hapten digoxigenin.The result of colony hybridization demonstrated that 2.8 kb probe reacted with strains of Y.enterolitica (different serotypes)and Y.pseudotuberculosis which expressed VW antigen,but it did not react with S.sonni,EIEC and E.coli.The above-mentioned results suggested that 2.8 kb probe have high specificity and be not related to Sereny test of both Shigella and EIEC.The probe would be very prospective in detecting the virulent strains of Yersinia.

本研究采用分子生物学方法从0:8型小肠结肠炎耶氏菌(Ye菌)所携带的毒力大质粒上分离、纯化了与Sereny试验相关的毒力基因2.8kb片段,经半抗原标记作为探针。菌落原位杂交结果证明2.8kb探针可与含表达VW抗原质粒的不同型Ye菌和假结核杆菌特异性杂交,与宋氏痢疾、EIEC和大肠杆菌无交叉反应。表明2.8kb探针有高度的特异性,且它与痢疾杆菌和EIEC的Sereny反应无关,在检测耶氏菌毒株方面将成为一个很有发展前景的DNA探针

 
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