Methods: The growth inhibition rates of human bladder cancer cell line BIU 87 by various concentration As 2O 3 were studied by MTT method, cell apoptosis of BIU 87 cells was observed by SABC immunohistochemical method.
To study the resistance mechanism of human bladder cancer cell line, an adriamycin(ADM) resistance bladder cancer cell line, BIU 87/A and an etoposide (VP 16) resistance cell line, BIU 87/V were established by exposing the BIU 87 parent cells to a culture medium with a persistent increase of concentration of ADM or VP 16 respectively over a period of 10 months.
OBJECTIVE:To investigate the effects of different concentrations of interferon-α(IFN-α) on human bladder cancer cell line T24 and on 25 patiants with bladder cancer.
HyTK gene,a fusion gene encoding both hygromycin phosphotransferase (hpt)and herpes simplex virus type 1 thymidine kinase (KSV 1 TK),was transduced into human bladder cancinoma cell line EJ by using recombinant retroviral vector.
Preliminary study of the in vitro growth inhibition of human bladder cancer cell line BIU-87 by arsenic trioxide
The growth inhibition rates of human bladder cancer cell line BIU87 by various concentrations of As2O3 were detected by using MTT method.
Comparison of the cytotoxic activities of chemotherapeutic drugs using a human bladder cancer cell line
A human bladder cancer cell line was exposed to a range of concentrations of the four drugs commonly used to treat superficial bladder cancer (adriamycin, epodyl, mitomycin-c, thiotepa) for periods of 30, 60 and 120 min.
Drug sensitivity of the human bladder cancer cell line J-82 was assessed using monolayer, stem cell and [3H]thymidine incorporation assays.
Using Northern and dot blot analysis, we investigated the expression of c-myc, c-fos abd erbB and regulation of oncogenes by TPA in BIU-87 cells a human bladder transitional cell carcinoma line We found expression of these oncogenes and induc-tion of these genes by TPA.We also found high expression of c-myc, c-fos, erbB, N-ras in human bladder cancer tissues.In contrast, only low level expression of N-ras was detected in normal bladder tissues.These data suggest that the expression of some oncogenes can be modulated...
Using Northern and dot blot analysis, we investigated the expression of c-myc, c-fos abd erbB and regulation of oncogenes by TPA in BIU-87 cells a human bladder transitional cell carcinoma line We found expression of these oncogenes and induc-tion of these genes by TPA.We also found high expression of c-myc, c-fos, erbB, N-ras in human bladder cancer tissues.In contrast, only low level expression of N-ras was detected in normal bladder tissues.These data suggest that the expression of some oncogenes can be modulated in a protein kinase C dependent manner and high level expression of some oncogenes are responsible for the abnormal growth in bladder carcinoma.
The cDNA of hGM CSF was transfered into the human bladder cancer line BIU 87 with retroviral vector. PCR southern blot demonstrated the integration of transfered gene in the BIU cell genome. Biological activity of the hGM CSF secreted by the BIU cell was confirmed. DNA analysis by flowcytomerty showed no influence on growth of the BIU cells. Flourenscent immunoassay revealed no induction of HLA ABC, DR, DQ expression on the surface of the gene transfered BIU cells. The gene transfected BIU cells were irradiated...
The cDNA of hGM CSF was transfered into the human bladder cancer line BIU 87 with retroviral vector. PCR southern blot demonstrated the integration of transfered gene in the BIU cell genome. Biological activity of the hGM CSF secreted by the BIU cell was confirmed. DNA analysis by flowcytomerty showed no influence on growth of the BIU cells. Flourenscent immunoassay revealed no induction of HLA ABC, DR, DQ expression on the surface of the gene transfered BIU cells. The gene transfected BIU cells were irradiated with 6000 rad X ray but they could secret hGM CSF for at least 2 weeks, although the growth of them was abrogated. These suggested that we could develope the bladder cancer vaccine secreting hGM CSF for clinical trials in future.
A human bladder cancer cells line resistant to doxorubicin, BIU-87/ADM,has been established in vitro by exposing BIU-87 parent cell to progressively higher concentrations of the drug over a period of 8 months. The cell line has been characterized in terms of growth kinetics, morphology, cross-resistance to other anticancerous agents, pharmacokinetics of ADM and expression of p-glycoprotein(P-gp)which is closely related to the MDR phenotype, The BIU-87/ADM cell was 6.3 times more resistant to doxorubicin than...
A human bladder cancer cells line resistant to doxorubicin, BIU-87/ADM,has been established in vitro by exposing BIU-87 parent cell to progressively higher concentrations of the drug over a period of 8 months. The cell line has been characterized in terms of growth kinetics, morphology, cross-resistance to other anticancerous agents, pharmacokinetics of ADM and expression of p-glycoprotein(P-gp)which is closely related to the MDR phenotype, The BIU-87/ADM cell was 6.3 times more resistant to doxorubicin than the BIU-87 parent cells. BIU-87/ADM exhibited cross-resistance to doxorubicin derivatives(epirubicin, daunorubicin),vincristine and etoposide, but not to cisplatin and mitomycin C, Compared to the parent cells, the resistant cells have a slower growth rate and lower confluent density. Unlike the BIU-87 parent cells, about 75% of the BIU 87/ADM cells showed a positive reaction with monoclonal antibody against P-gp,JSB-1. Intracellular drug accumulation studies with fluorescence spectrometry indicated that the resistance exhibited by the BIU-87/ADM line was mainly caused by an increased active efflux, These results suggest that MDR is an important phenomenon in bladder cancer and that more than one pathway of MDR may be present in human bladder cancer cell lines. BIU-87/ADM may be a useful model for the development of new chemotherapeutic strategies in overcoming drug-resistance in the treatment of bladder cancer.
人膀胱癌细胞株
human bladder cancer cell line
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