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酶活回收率
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  enzyme recovery
     The magnetic polymer microspheres were also modified with flexible and hydrophilic DAS. After being bound with papain we obtained the immobilized enzyme. All the results showed that the enzyme recovery increased greatly with amount of added enzyme decreasing, and K_m also reduced, which illustrate that the enzyme affinity to substrate improved.
     进一步将这种磁性高分子多孔复合微球胺化后修饰上柔顺、亲水的双醛淀粉链(DAS)再与酶结合制得固定化酶,结果发现单位固定化酶量略有减少,酶活回收率提高,米氏常数(K_m)较游离酶减少,酶对底物的亲和力提高。
短句来源
     The method possesses the practical value for its advantages of simple operation,short separating cycle and high enzyme recovery.
     该法步骤简单、分离周期短、酶活回收率高、具有较大的实用价值。
短句来源
     The method possesses the practical value for its advantages of low production cost,simple operation and high enzyme recovery. 
     本方法提取成本低,操作简便,酶活回收率高,因此具有应用价值
短句来源
  “酶活回收率”译为未确定词的双语例句
     D-hydantoinase was separated and purified from Burkholderic cepecia 1003 following the steps of the crude separation(the cellular fragmentation 、the precipitation of (NH_4)_2SO_4), the purification of chromatography(Phenyl CL-4B HIC、DEAE Sepharose Fast Flow negative IEX) in this experiment. The purification multiple was 25.11 and the recycle yield of enzymatic activity was 11.87%.
     本实验将Burkholderic cepecia 1003 菌株发酵产菌经过粗分离(细胞破碎、(NH_4)_2SO_4沉淀)、层析纯化(Phenyl CL-4B疏水层析、DEAE Sepharose Fast Flow阴离子交换层析)等步骤后得到纯化好的D-海因酶,其纯化倍数为25.11,酶活回收率为11.87%。
短句来源
     The suitable extraction conditions are 27 % PEG1000,13 %(NH_4)_2SO_4,pH 7.5.It was shown that the partition coefficient of K_(e) of (10.246 2),K_(p) of 2.316 0,the yield of 94.8 %.
     实验表明当萃取体系中PEG1000、(NH4)2SO4的质量分数为27%和13%,pH值为7.5时,分配系数Ke、酶活回收率η和总蛋白的分配系数Kp分别为10.246 2、94.8%和2.316 0.
短句来源
     The result showed that the yield of enzyme activity of 94.84% and the partition coefficient of 17.10 could be obtained under the conditions of PEG400 21%~22%(w/w)and(NH4)2SO4 14%~17%(w/w)at room temperature.
     实验表明,由PEG40021%~22%(W/W)和(NH4)2SO414%~17%(W/W)所组成的双水相体系,在室温下对α-淀粉酶的酶活回收率可达94.84%,分配系数可达17.10。
短句来源
     The flow rate was 5mL/min and the injection volumn was 15mL, the final purification factor was 2.4 and the recovery of Nattokinase was 71%.
     将透析后的发酵液上SP Sepharose Fast Flow离子交换柱进行梯度洗脱,流速为5mL/min,上样量为15mL,最终的纯化倍数为2. 4,酶活回收率为71%。
短句来源
     The optimal conditions of coupling reaction were pH8.0 and the amount of trypsin 7.26 mg/ml,the activity of immobilized trypsin and recovery rate were 98.3 U/g(cellulose) and 57.5% respectively.
     在偶联反应pH8.0、酶量7.26mg/ml的最佳固定化条件下,固定化酶活力达98.3U/g(湿纸纤维),酶活回收率为57.5%。
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     The highest enzyme activity retained 30.2%.
     最高回收率为30.2%。
短句来源
     The maximum yield of immobilized enzyme was 30.2 %.
     固定化最高回收率为30.2%.
短句来源
     after lh,the activity of modified EFE is 63.6% of free EFE, and the modification rate of amino residues is 72.1%.
     46X1了川mg,回收率为63.6%。
短句来源
     After the three-step treatment, purification fold is 6.7 with 67.14% enzyme activity recovery and single band shows by PAGE determination.
     纯化倍数达到6.7,回收率为67.14%。
短句来源
     Methods SOD, POD and CAT enzymes analysis.
     方法 分析技术。
短句来源
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  enzyme recovery
Enzyme recovery in high-solids enzymatic hydrolysis of steam-pretreated willow: Requirements for the enzyme composition
      
It was found that ammonium sulphate at a saturation of 0.3-0.5 gave the maximum enzyme recovery (88.8%) from the crude extract, while acetone gave 66.2% at a concentration of 3/1 (V/V).
      
A model was used to represent the enzyme recovery and fit of the experimental data.
      
It is likely that enzyme recovery and reuse during the hydrolysis of posttreated softwood substrates could lead to reductions in the need for the addition of fresh enzyme during softwood-based bioconversion processes.
      
Enzyme recovery and recycling following hydrolysis of ammonia fiber explosion-treated corn stover
      
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This paper reported an immobilization method for whole cell of DNA recombinant strain expressing penicillin G acylase by algin (sodium alginate).The immobiling yield for E. Coli is about 30-40%. The recovery of enzyme activity is about 88%. The PDAS method for determination of 6-APA is improved in this paper too.

本文报告以褐藻胶(海藻酸钠)固定化青霉素酰化酶工程菌的方法。本方法对大肠杆菌的包埋量可达30~40%(W/W),酶活回收率为88%。本文对6-APA测定的PDAB法进行了改进;对固定化细胞酶活的测定提出一种新方法。

The influences of molecular weight of PEG, concentrations of PEG and potassium phosphate, the amounts of protein and concentration efficiency were studied. The optimum conditions of aqueous two-phase systems for separating aspartase, cellulase and lipase from the crude aspartase, industrial cellulase and lipase respectively are devised.

用聚乙二醇(PEG)/磷酸钾双水相抽提技术分别从天冬氨酸粗酶、商品纤维素酶和脂肪酶中分离纯化出天冬氨酸酶(Aspartase)、纤维素酶(Cellulase)和脂肪酶(Lipase)。研究了PEG分子量及浓度、磷酸钾浓度、蛋白质含量、pH、氯化钠浓度等因素对分配系数,酶活回收率和分离效果等参数的影响,并设计出双水相体系抽提三种酶的相组成系统。

A porous hydrophilic immobilized support-Methdcrylate reain was synthesized by sus-pension technique. Immobilized glucoamylase with the resin was prepared by the protectiveglutaraldehyde,diazotization and Curtius azide methods respectively.The effects of immobilizedglucoamylase activity on the molar ratio of glutaraldehyde and diethanol amine, the differentparameters of porous structure, three active resin operations and spacers of reain were also re-ported. The results showed that the protective glutaraldehyde...

A porous hydrophilic immobilized support-Methdcrylate reain was synthesized by sus-pension technique. Immobilized glucoamylase with the resin was prepared by the protectiveglutaraldehyde,diazotization and Curtius azide methods respectively.The effects of immobilizedglucoamylase activity on the molar ratio of glutaraldehyde and diethanol amine, the differentparameters of porous structure, three active resin operations and spacers of reain were also re-ported. The results showed that the protective glutaraldehyde method for immobilizedglucoamylase was satisfied when the molar ratio of glutaraldehyde and dicthanol amine was 4∶1and the average pore diameter of resin was 552.1A. The recovery of the relative and total activityof the immobilized enzyme is 55.6% and 24.7% respectively.

用悬浮聚合法合成了多孔性甲基丙烯酸树脂。用此载体比较了保护戊二醛法,重氮化法和叠氮法固定化葡萄糖淀粉酶的效果,并研究了二乙醇胺保护戊二醛活化载体,树脂多孔结构多数及不同悬臂长度等方面对固定化葡萄糖淀粉酶活力的影响。结果表明,用保护戊二醛法活化的树脂固定化酶效果较好。当戊二醛与二乙醇胺为4∶1(摩尔比),树脂孔径为552.1,比表面为28.3m ̄2/g孔容为0.635cm ̄3/g时,固定化酶的相对活力为55.6%,而酶活回收率为24.7%。

 
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