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灰色链霉菌
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  streptomyces griseus
     Molecular Biology Study of Cholesterol Oxidase Gene of Streptomyces Griseus ATCC14811
     灰色链霉菌ATCC14811胆固醇氧化酶基因的分子生物学研究
短句来源
     A STUDY ON TRANSFORMATION OF STREPTOMYCES GRISEUS PLASMID DNA
     灰色链霉菌质粒DNA转化的研究
短句来源
     Purification and Some Properties of Bacteriolytic Enzyme R1 from Streptomyces griseus RX-17
     灰色链霉菌RX-17溶菌酶R1的纯化及性质研究
短句来源
     Comparative Studies between Streptomyces sp. FR-008 and Streptomyces Griseus IMRU3570 and Their Metabolites
     链霉菌FR-008与灰色链霉菌IMRU3570及其代谢产物的比较研究
短句来源
     Based on the polyphasic studies including its morphology, physiological and biochemical characteristics, chemotaxonomy and 16S rDNA sequence analysis, and comparison with genus Streptomyces in the utilization of carbon source in Genebank, Strain HG2393 was a new subspecies of genus Streptomyces and named as Streptomyces griseus subsp.
     其培养特征、生理生化特性和16S rDNA序列均与灰色链霉菌(Streptomyces griseus)极为相似。 但是,HG2393与《放线菌的分类和鉴定》中几种较典型灰色链霉菌在碳源利用等生理生化特征存在显著差异,故认定菌株HG2393为灰色链霉菌(Streptomyces griseus)的新亚种,定名为灰色链霉菌杭州亚种(Streptomyces griseus subsp. hangzhouensis)。
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  “灰色链霉菌”译为未确定词的双语例句
     The Streptomyces gresius RX-17 producted high activityN,O-diacetylmuramidase, which possessed not only β-1,4-N,6-O-diacetylmuramidaseactivity but also β-1,4-N,6-O-diacetylmuramidase activity.
     灰色链霉菌(Streptomyces griseus)RX-17所产的溶菌酶系,不仅具有β-1,4-N-乙酰胞壁质酶活力,还具有β-1,4-N,6-0-二乙酰胞壁质酶活力。
短句来源
     The flocculability of the penicillium ferment and the actinomycetes ferment are 68.4% and 69.0% respectively.
     青霉发酵液和灰色链霉菌发酵液絮凝率最高可分别达到68.4%和69.0%。
短句来源
     According to shotgun method, Sau3AI cutted the genome of S.gresius incompletely, then 2-10kb fragment was linked with the linear plasmid pUC19 digested by BamHI and CIAP completely. Then the recombination plasmid transformed into competence cell of E.
     限制性内切酶Sau3AI对灰色链霉菌(S.griseus)RX-17基因组DNA进行不完全酶切,回收2-10kb的DNA片段:回收片段与经BamHI酶切、CIAP去磷酸化处理后的pUC19连接,构建重组质粒,转化E.
短句来源
     A 1-Kb PCR product was isolated by means of PCR using the total DNA of S. griseus as template. The product was ligated into pGEM-3Zf(+), leading to construct a plasmid, designated pLZl.
     通过PCR方法以灰色链霉菌总DNA为模板克隆到一个1Kb大小的DNA片段,连接到pGEM-3Zf(+),构建了pLZ1。
短句来源
     Plasmid in Relation to Streptomycin Biosynthesis----Isolation and Characterization of 5. griseus Plasmids before and after Transformation
     链霉素生物合成有关质粒的研究——转化前后灰色链霉菌质粒的检测
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  相似匹配句对
     A NEW SUBSPECIES OF STREPTOMYCES GRISEUS
     灰色链霉菌的一个新亚种
短句来源
     MUTAGENIC BREEDING OF STREPTOMYCES GRISEUS WITH FAST NEUTRON
     灰色链霉菌快中子诱变育种
短句来源
     Colorful Shades of Gray
     绚烂的灰色
短句来源
     On grey function
     灰色函数
短句来源
     C1027, produced by S. globisporus C1027, is a new macromolecular antitumor antibiotic composed of an enediyne chromophore and an apoprotein through non-covalent bond binding.
     C1027是从链霉菌S.
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  streptomyces griseus
Streptomyces griseus 1439, which forms aerial mycelium and spores only in the presence of exogenous A-factor, was used as the test strain.
      
Among the 28 spontaneous variants, three new A-factor-dependent strains were revealed, which represented the species Streptomyces griseus, "S.
      
None developed on Streptomyces griseus (Krainsky) Waksman >amp;amp; Henrici.
      
Streptomycinempfindlichkeit der Sporen von Streptomyces griseus
      
Cytologische Untersuchungen an Streptomyces griseus (Krainsky)
      
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Direct transformation of Streptomyces griseus No.45-3 (Lin5, Penr, Amy- , Str- ) was performed successfully with the plasmid DNA isolated from S. griseus No. 45 (Lin, Pen5, Amy+. Str+), a streptomycin producing strain. Lincomycin resistance was used as a selective marker. Optimal conditions for transformation have been determined: recipient cell in early logarithmic phase, DNA treatment 60 minutes, and CaCl2 concentration in the transformation liquid 10-6 M. Among the lincomycin resistant transfor-mants selected...

Direct transformation of Streptomyces griseus No.45-3 (Lin5, Penr, Amy- , Str- ) was performed successfully with the plasmid DNA isolated from S. griseus No. 45 (Lin, Pen5, Amy+. Str+), a streptomycin producing strain. Lincomycin resistance was used as a selective marker. Optimal conditions for transformation have been determined: recipient cell in early logarithmic phase, DNA treatment 60 minutes, and CaCl2 concentration in the transformation liquid 10-6 M. Among the lincomycin resistant transfor-mants selected about 65.9 % resumed the ability of streptomycin biosynthesis. These results further verified that plasmid is involved in streptomycin biosynthesis in S. griseus.

我们以链霉素产生菌——灰色链霉菌No.45为给体(Lin~r、Pen~s、Amy~+、Str~+),以高温消除质粒的No.45-3(Lin~s、Pen~r、Amy~-、Str~-)为受体,以Lin~r为筛选标记,进行转化试验。从中摸索出一套最适的转化条件:感受态为对数生长初期(5.5小时),转化液中含有10~(-6)M的Ca~(2+),转化处理时间为60分钟。结果在灰色链霉菌中,用抗性标记直接转化获得成功,而且有65.9%的转化子已恢复了链霉素生物合成的能力。这就更进一步证明质粒与链霉素生物合成有关。

The streptomycin molecule is composed of three moieties i.e.streptose,streptidine and N-methyl-L-glucosamine.All the aerial mycelium nagative mutants of Streptom-yces griseus obtained in our laboratory have lost the ability to synthesize streptidine but retained the ability to synthesize streptose and N-methyl-L-glucosamine.Streptomycin cannot be synthesized by the addition of streptidine alone to the fermentation cultures of mutant strain No.45-3 in the presence of ATP and Mg2+However,when both streptidine...

The streptomycin molecule is composed of three moieties i.e.streptose,streptidine and N-methyl-L-glucosamine.All the aerial mycelium nagative mutants of Streptom-yces griseus obtained in our laboratory have lost the ability to synthesize streptidine but retained the ability to synthesize streptose and N-methyl-L-glucosamine.Streptomycin cannot be synthesized by the addition of streptidine alone to the fermentation cultures of mutant strain No.45-3 in the presence of ATP and Mg2+However,when both streptidine and streptidine phosphatase are added either to fermentation cultures or to cell-free extracts of the mutant strain No.45-3 in the presence of ATP and Mg2+,the biosynthesis of streptomycin is resumed.This indicates that S.griseus plasmid (s) codes streptidine phosphatase gene as well as some other involved in streptidine biosynthesis.

链霉素是由链霉糖、链霉胍和N甲基一L一葡萄糖胺三部分组成。我们证明了经高温消除质粒后不产链霉素的突变株不能合成链霉胍。在补加外源链霉胍、链霉胍磷酸酯酶、并有ATP和Mg~(2+)存在的条件下,由链霉胍磷酸酯酶催化外源链霉胍转变为O-磷酸链霉胍,进入链霉素的正常生物合成途径后产生链霉素。本文证明了灰色链霉菌质粒编码链霉胍磷酸酯酶的基因以及链霉胍合成的有关基因。

The microbes that are able to deteriorate raw rubber and vulcanizates, have been isolated, screened and identified. Among them there are 5 bacterial species(Bacillus lichenformis, Achromobacter liquefaciens, Alcaligenes afaecalis etc), 7 fungi (Aspergillus flavus, Penicillium chrosogenum etc), and9 species of actinomyces. Mixed strains are more resistant toward microbicides than single strain and rubbers are more sensitive to microbial attack of mixed strains than to that of a single strain. The bateria are...

The microbes that are able to deteriorate raw rubber and vulcanizates, have been isolated, screened and identified. Among them there are 5 bacterial species(Bacillus lichenformis, Achromobacter liquefaciens, Alcaligenes afaecalis etc), 7 fungi (Aspergillus flavus, Penicillium chrosogenum etc), and9 species of actinomyces. Mixed strains are more resistant toward microbicides than single strain and rubbers are more sensitive to microbial attack of mixed strains than to that of a single strain. The bateria are most resistant to microbicides, with fungi being less and actinomyces the least resistant. The tendency of anti-microbial attack of rubber increases in order of natural rubber, Buna S, butylrubber, neoprene, Buna N, thiokol in raw rubber; and Buna S, neoprene, Buna N in vulcanized rubber. According to microbicidal toxicity, the durability, the reaction with compounding ingredient, the effect of biocides on physical mechanical properties, ageing of rubber, cheap and low toxic, the microbicide Zn-pentachlorophenate(PR2)is selected from more than 40 microbicides. We suggest that PR2 is applied as microbicide for protection from microbial attack of rubber used in waterproof materials underground. The Buna N incorporated with a concentration 2-5% of PR2 possesses not only excellent anti-microbial potency, but also desirable physical mechanical properties.

通过对生胶、硫化胶上侵蚀橡胶微生物的分离、筛选、鉴定、获得了攻击橡胶能力强的细菌有地衣芽孢杆菌(Bac. lichenformis)等5个种;霉菌有黄曲霉(Asp. flavus)等7个种及放线菌有灰色链霉菌(S. griseus)等9个种。在侵蚀橡胶能力及耐药性上,混合菌都比单一菌强。抗药性以细菌最强,霉菌次之,放线菌最弱。橡胶抗菌侵蚀性能,生胶按天然胶、丁苯胶、丁基胶、氯丁胶、丁睛胶、聚硫胶顺序;硫化胶按丁苯、氯丁、丁睛橡胶次序递增。按杀菌剂的杀菌力、持久性、同橡胶配合剂反应及对橡胶物理机械、老化等性能影响,及从价廉、低毒及稳定性比较,最终推荐用五氯酚锌(PR_2)为橡胶杀菌剂。加2—5%该药剂的防水丁睛胶有良好抗菌性,也不影响其物理机械性能。

 
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