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杂交基因
相关语句
  hybridization gene
     Research about subtraction hybridization gene clone
     消减杂交基因克隆技术研究进展
短句来源
     The technique of gene clone is very useful to explore the molecular mechanism of life. By using technique of subtraction hybridization gene clone,great progress in screening and orientating for some multigenic disease realated gene was made.
     基因克隆技术是研究生命过程分子机制的基础,消减杂交基因克隆技术的应用,使一些多基因遗传病相关致病基因的筛查和定位有所突破。
短句来源
  “杂交基因”译为未确定词的双语例句
     Methods The cervical specimens from 356 patients with HPV infection were collected, Hybrid Capture Ⅱ (HCⅡ) assay was used to detect the 13 HPV-H types, and DNA flow-through hybridization genotyping technique was applied to determine the 21 HPV-DNA types.
     方法研究对象系356例宫颈HPV感染的患者。 以第二代杂交捕获(HCⅡ)试验检测13种HPV-H,同时采用核酸分子快速杂交基因分型技术进行21种HPV-DNA亚型分析;
短句来源
     Application research on the flow-through hybridization and gene chip in human papillomavirus detection
     导流杂交基因芯片技术在人乳头状瘤病毒检测中应用的研究
短句来源
     Results Of the 300 sera genotyped by PCR-RBD, 147 (49.0%) cases were genotype B, 136 (45.3%) were genotype C, 1 (0.3%) genotype D, and 12 (4.0%) were mixtures of genotype B and C, and 4 (1.3%) were mixtures of genotype C and D.
     结果 新建HBV逆向点杂交基因分型方法可对拷贝数在103~109/ml之间的300份HBV DNA阳性抽检血清进行基因分型,发现B型147例,占49.0%; C型136例,占45.3%;
短句来源
     Under conditions of no embryo culture,it was studied that crosses, backcrosses and self-fertilizations between Aegilops species as female and common wheat without recessive crossability gene.
     研究了山羊草不同物种作母本,在不借助胚培等特殊措施的情况下与不含隐性可杂交基因的小麦推广品种杂交、回交及杂种自交情况,结果表明:(1)山羊草物种作母本,小麦推广品种作父本进行杂交是一种很有效的方式;
短句来源
     Application of flow-through hybrization and gene chip on human papillomavious subtype detection in clinical
     导流杂交基因芯片技术在人乳头状瘤病毒感染分型检测中的临床应用
短句来源
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  相似匹配句对
     Green Revolution ——From Hybridization to Gene Engineering
     绿色革命——从杂交基因工程
短句来源
     4 new genes were obtained.
     U基因
短句来源
     In Situ Hybridization on Vasa Gene of Silkworm Bombyx Mori.
     家蚕vasa基因的原位杂交研究
短句来源
     The gene was highly expressed in a soluble form in E.
     该基因在大肠杆菌E.
短句来源
     northern hybridization.
     Northern杂交
短句来源
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  hybridization gene
Using somatic cell hybridization gene mapping methodology, genes coding for human cell-surface proteins have been assigned to specific chromosomes.
      


The Breeding work of Octoploid Triticale since 1957 is summarized as follows:1. The use of crossable gene. The genetic analysis of the crossability between common wheat and rye has shown that s, ss, SA, SN and SQ belong to a multiple allelic series of a single locus. The degree of crossability of these genes can be arranged in such an order, namely, s > ss > SA > SN > SQ. According to the degree of dominance, the order should be reversed as: SQ > SN > ss > SA > s. These findings were properly used in the routine...

The Breeding work of Octoploid Triticale since 1957 is summarized as follows:1. The use of crossable gene. The genetic analysis of the crossability between common wheat and rye has shown that s, ss, SA, SN and SQ belong to a multiple allelic series of a single locus. The degree of crossability of these genes can be arranged in such an order, namely, s > ss > SA > SN > SQ. According to the degree of dominance, the order should be reversed as: SQ > SN > ss > SA > s. These findings were properly used in the routine work of crosses between wheat and rye.2. Chromosome doubling. Before treatment the wheat-rye hybrid seedlings should be slightly wounded on crown part by a sharp razor blade. Then the hybrid seedlings are exposed in 0.04-0.05% aqueous solution of colchicine for 4 days in room temperature not over 15℃. More than 90% of the treated seedlings could be recovered in greenhouse below 10℃. Among the recovered seedlings, about 40.8% of the sterile F1 hybrid plants would be turned to be partial fertile and various amount of seeds could be obtained from these successful plants. By this procedure, 4,700 primary Triticale strains have been created. In 1961, a new polyploidizing agent was discovered. The name of the agent is Fumiron, or Phenyl mercury-p-toluene sulfonanilide which is a fungicide and has comparable cfficiency in chromosome doubling as colchicine does.3. Fertility and seed plumpness. It was suggested that recombination of genes by hybridization and molding the segregation generations by heavy selection pressure could be the effective measures for the improvement of the fertility and seed plumpness of the octoploid Triticale strains. About two thousand of cross-combinations have been made with various parental stocks which were mostly selected from the primary Triticale strains, and in recent years, hybrid strains and elite plants in segregation generation were used in crosses more frequently than the primary types. The hybrid strains thus developed with normal fertility and acceptible seed plumpness were released to different localities with various natural conditions for yield tests.4. Regional tests. The data collected from regional tests have shown a tendency that the Triticale hybrid strains at present state might be successful in those regions where the yield of common wheat is usually very low and unstable due to severe natural conditions. For example, 10 Triticale strains were tested for yield performance with one rye and two common wheat varieties as checks in 1972-1973 growing season in Weining, a mountainous region of Kweiehow with altitude between 2-3 thousand meters. Eight out of ten Triticale strains have higher yields than both rye and wheat varieties. The best strain, Triticale No. 2 has a yield about 20% higher than that of rye, and 24% and 61% higher than the two varieties of common wheat Ahpo and No. 778 respectively. However, it, should not thus be concluded that the octoploid Tri-ticale is especially suitable for the marginal habitats of common wheat. It is only apparently seeming so at the present state of the Triticale strains which were developed from selection solely directed to the improvement of fertility and seed plumpness without much consideration for other characteristics of agricultural importance. Now, only the hardiness from rye and good seed quality from common wheat generally incoporated in the amphiploid have shown their favourable effect in the marginal regions of these two crops. This might be considered therefore that it is merely a preliminary stage of the Triticale program.

从1957年以来的八倍体小黑麦育种工作的主要结果总结如下: 1.可杂交基因的应用 小麦和黑麦之间的可杂交遗传分析表明s,s~S,s~A,s~N和s~Q是属于一个基因座的复等位基因。根据可杂交的程度,这些基因可以排成如下的次序,即s>s~S>s~A>s~N>s~Q。根据显性的程度,则其次序就要倒过来成为:s~Q>S~N>s~S>s~A>s。这个发现已被适当地应用于小麦与黑麦的日常杂交工作中。 2.染色体数加倍 小麦-黑麦杂种分蘖苗于处理前在基部用刀片切一浅伤口,而后浸在0.04—0.05%的秋水仙精溶液中4天,室温保持在15℃以下。在10℃以下的温室中,90%以上的处理苗能恢复生长。恢复苗中约有40.8%的F_1不育杂种植株能转变成部分可育的,并以这些成功株上将获得数目不等的种子。用这个方法,曾经制造了4,700个小黑麦原始品系。在1961年,发现了一个新的多倍体诱变剂。药品的名字是富民隆,或称对甲苯磺硫苯胺基苯汞,它是一个杀菌剂,加倍染色体数的效果和秋水仙精一样。 3.结实率和种子饱满度 通过杂交的基因重组和加重分离世代的选择压力是改进八倍体小黑麦的结实率和种子饱满度的有效方法。从小黑麦原始品系中...

从1957年以来的八倍体小黑麦育种工作的主要结果总结如下: 1.可杂交基因的应用 小麦和黑麦之间的可杂交遗传分析表明s,s~S,s~A,s~N和s~Q是属于一个基因座的复等位基因。根据可杂交的程度,这些基因可以排成如下的次序,即s>s~S>s~A>s~N>s~Q。根据显性的程度,则其次序就要倒过来成为:s~Q>S~N>s~S>s~A>s。这个发现已被适当地应用于小麦与黑麦的日常杂交工作中。 2.染色体数加倍 小麦-黑麦杂种分蘖苗于处理前在基部用刀片切一浅伤口,而后浸在0.04—0.05%的秋水仙精溶液中4天,室温保持在15℃以下。在10℃以下的温室中,90%以上的处理苗能恢复生长。恢复苗中约有40.8%的F_1不育杂种植株能转变成部分可育的,并以这些成功株上将获得数目不等的种子。用这个方法,曾经制造了4,700个小黑麦原始品系。在1961年,发现了一个新的多倍体诱变剂。药品的名字是富民隆,或称对甲苯磺硫苯胺基苯汞,它是一个杀菌剂,加倍染色体数的效果和秋水仙精一样。 3.结实率和种子饱满度 通过杂交的基因重组和加重分离世代的选择压力是改进八倍体小黑麦的结实率和种子饱满度的有效方法。从小黑麦原始品系中选用各种亲本大约已经做了两千个杂交组合,近年来更多的是用杂种选系和分离世代中好的植株来进行杂交。由此而选育出来的,结实率正常,

To investigate the crossabilities of wheat(T.aestivum)with bulbousbarley(H.bulbosum)and their inheritance,42 wheat varieties,9 F_1 and1 F_2 intervarietal crosses were used as maternal parents in crossing withbulbous barley of Soviet and Hungarian origins(2n=28)and their clo-nes.Part of the wheat varieties were also crossed with rye(Secale ce-reale).The results obtained indicated that crossability of wheat withbarley was similar to that with rye and there were no significant diffe-rences for crossability among...

To investigate the crossabilities of wheat(T.aestivum)with bulbousbarley(H.bulbosum)and their inheritance,42 wheat varieties,9 F_1 and1 F_2 intervarietal crosses were used as maternal parents in crossing withbulbous barley of Soviet and Hungarian origins(2n=28)and their clo-nes.Part of the wheat varieties were also crossed with rye(Secale ce-reale).The results obtained indicated that crossability of wheat withbarley was similar to that with rye and there were no significant diffe-rences for crossability among barley germplasms and clones of differentorigins.Gibberellic acid treatment of pollinated florets promoted thecrossability of the crossable varieties and the development of haploidembryos,but there was no effect on the non-crossable varieties.Thecrossability of wheat with bulbous barley was controlled by a pair ofgenes,the readily crossable character being recessive,while the non-crossable being dominant,and the degree of dominance varied withwheat varieties.It is concluded that the crossabilities of wheat witheither rye or bulbous barley are conditioned by the same genetic systemin wheat,and the similarity in crossabilities of wheat with either spe-cies is an expression of homologous variation in crossability originatedfrom a close relationship between the two species.It is also considered that bulbous barley is not likely to use widelyin wheat breeding unless the cross able genes are introduced into a ma-jority of wheat varieties or a new barley line is found that can overcomethe incompatibility with wheat.

选用了42个普通小麦品种、9个品种间杂种 F_1和一个 F_2作母本,分别与苏联球茎大麦和匈牙利球茎大麦及其不同株系杂交,其中的部分品种还同黑麦进行了杂交,以研究小麦与球茎大麦的可杂交性及其遗传规律。结果表明,小麦与球茎大麦的可杂交性同小麦与黑麦的情况相似,供试的两个球茎大麦及其株系间在可杂交性上无显著差异存在。授粉后喷射赤霉酸能提高易杂交品种的杂交率和促进胚的发育,但对不能杂交的品种无效。结果还表明可杂交性受一对基因支配,易杂交为隐性,不易杂交为显性,显性程度随小麦品种不同而异。根据试验结果和有关研究,作者认为,除非能把易杂交基因代换进大部分小麦品种中去,或能找到易与多数小麦品种杂交的球茎大麦,否则,球茎大麦技术不可能在小麦育种上广泛应用。作者推测,小麦与球茎大麦和黑麦的可杂交性在很大程度上是由小麦的同一遗传系统控制的,并认为小麦与这两个种的可杂交性相似是由于黑麦和球茎大麦有着较密切的亲缘关系而表现出的同源变异。

An easy and simple method was described here for both the survey of β—Thal and further analyzing the spectrum of β—Thal mutations by collecting human peripheral blood for once only. The qualitative osmostic fragility test (QOFT screening in 0. 32% NaCl) and Hb micro—CAM eletrophoresis were used for screening out the cases of β—Thal first, after the DNA was isolated from the pellet of white cells, then analyzed by the raised HbA_2 can be visualzed on the Hb electropherogram and if the amount of it is over 3.5%...

An easy and simple method was described here for both the survey of β—Thal and further analyzing the spectrum of β—Thal mutations by collecting human peripheral blood for once only. The qualitative osmostic fragility test (QOFT screening in 0. 32% NaCl) and Hb micro—CAM eletrophoresis were used for screening out the cases of β—Thal first, after the DNA was isolated from the pellet of white cells, then analyzed by the raised HbA_2 can be visualzed on the Hb electropherogram and if the amount of it is over 3.5% (or 4. 0% in malaria current zone) it can be detected by the method of DEAE microcolumn chromatography. Totaled 730 primary students of the Li nationality in Juntian village, Hainan Island, were investigated, and 49 cases of β—Thal were screened out by the QOFT and the Hb electropherogram. Among the 49 cases 28 were randomly chosen for further analyzed by the PCR—ASO method. 27 cases of β—Thal genotype (CD41/42,—TCTT) were found, one of them was unidentified, and the credible rate of screening out β—Thal by the QOGT and the Hb electropherogram was over 96%. The another 6 cases of non—β—Thal were tested by the QOFT and Hb electropherogram it were further proceed not of β—Thal genotype by the PCR—ASO method. A detail discusion was gaven in this paper about the survey of the β—Thal with the PCR—ASO gene diagnosed bythe PCR—ASO method.

本文探讨β—地中海贫血人群普查和基因分析的方法。采一次未梢血,完成β—地中海贫血对象调查和基因类型分析:检查项目:红细胞脆性试验(0.32%Nacl溶液,一管法),Hb微量CAM电泳、目测Hb电泳图判断HbA_2是否增高,DEAE微量柱层析法测定HbA_2含量;白细胞部分供提取DNA进行PCR扩增及ASO探针杂交基因分析。红细胞脆性试验阳性及HbA_2含量超过3.5%(疟疾高发区>4%)者为β—地中海贫血对象。将这些对象的末梢血提取DNA,做PCR扩增及ASO探针杂交法基因分析。普查军田乡黎族小学生730人,筛出β—地贫对象49人。随机用PCR—ASO法检测28例β—地贫对象,检出β—地CD41/d2(—TCTT)基因型杂合子27例,未查明1例,符合率达96%以上。非β—地中海贫血6例经PCR—ASO法检测均非β—地贫基因变异。本文对β—地中海贫血普查结合PCR—ASO基因诊断的方法进行了详细讨论。

 
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