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   杂交组织化学 的翻译结果: 查询用时:0.043秒
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杂交组织化学
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  hybridization histochemistry
     The distribution of PcR 18 gene expressing cells in the rat retina was examined by means of in situ hybridization histochemistry method (ISHH).
     为了解PcR-18基因在大鼠视网膜上的细胞定位,用原位分子杂交组织化学方法(ISHH),观察了代号PcR-18的视网膜特异基因表达细胞在大鼠视网膜内的分布。
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     Hematoxylin-esoin (HE) staining, Masson staining and TGFβ1 mRNA, type Ⅰ and Ⅲ collagen mRNA in situ hybridization histochemistry staining were applied.
     采用苏木精-伊红染色、Masson染色及转化生长因子β1mRNA、Ⅰ、Ⅲ型胶原mRNA的原位杂交组织化学染色。
短句来源
     METHODS: The distribution of endothelin-1 and the expression of ET-1 mRNA in the rabbits uterine tubes were studied using SABC immunohistochemistry and in situ hybridization histochemistry with digoxigenin-labelled rat ET-1 cRNA probe.
     方法 :用SABC免疫组织化学法和地高辛精标记的ET - 1cRNA探针核酸原位杂交组织化学法 ,观察了兔输卵管组织ET - 1的分布和ET - 1mRNA的表达。
短句来源
     By means of in situ hybridization histochemistry (ISHH), the distributive particularities in time and location of the early expression of ET 1 messenger ribonucleic acid (ET 1 mRNA) in injured spinal cord tissues from rats were qualitatively and quantitatively measured.
     借助原位杂交组织化学方法,定位、定量研究大鼠急性脊髓损伤后早期ET- 1 mRNA表达的时间和空间分布特征。
短句来源
     Using in situ hybridization histochemistry, we examined the localization of the neurons containing calbindin-D_(28K) mRNA in the rat brainstem.
     用原位杂交组织化学技术,对含Calbindin-D28K mRNA的神经元在大鼠脑干中的分布进行全了面观察。
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  hybridization combined
     Methods Situ hybridization combined with computer image analysis was used to exame the expression, volume density (Vv) and numerical density (Nv) of the SSmRNA positive cells in three groups of model rats, ie the regular electric and irregular flashing light stimulating group (group A), the regular electric and regular flashing light stimulating group (group B) and the only irregular flashing light stimulating group (group C).
     方法 采用心理应激大鼠模型A、B、C三组, 以原位杂交组织化学和微机图像分析技术, 检测大鼠心理应激后胃窦粘膜生长抑素mRNA 阳性反应细胞的表达强度和细胞体积密度(Vv) 、单位体积数密度(Nv) 的变化。
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  “杂交组织化学”译为未确定词的双语例句
     CHANGES OF GABA_A RECEPTOR γ_2 SUBUNIT IN HIPPOCAMPUS OF CHRONIC EPILEPTIC RATS──A STUDY OF IN SITU HIBRIDIZATION HISTOCHEMISTRY
     慢性癫痫大鼠海马内GABA_A受体γ_2亚单位的改变──原位杂交组织化学研究
短句来源
     The root tips of 3-d-old maize seedlings (about 1cm) are treated by antiauxin (clofibric acid 10-7 mol/L, 10-6 mol/L, 10-5 mol/L) one day, in situ hybridization is used to identify the influence of endogenous auxin in root tip on the expression of root cap specific gene (Clone C103).
     系列浓度抗生长素(降固醇酸0.1μmol/L、1.0μmol/L、10μmol/L)处理3-天龄玉米幼苗根尖(约1cm)1天,利用原位杂交组织化学方法鉴定根尖内源生长素对根冠特定基因(CloneC103)表达产生的影响。
短句来源
     LOCALIZATION OF NEURONS CONTAINING CALBINDIN-D28K mRNA IN THE RAT SPINAL CORD── AN IN SITU HYBRIDIZATION ANALYSIS
     含Calbindin-D28K mRNA神经元在大鼠脊髓中的分布──原位杂交组织化学法观察
短句来源
     LOCALIZATION OF THE NEURONS CONTAINING CALBINDIN-D28K mRNA IN THE RAT FOREBRAIN
     含Calbindin-D28K mRNA的神经元在大鼠前脑中的分布—原位杂交组织化学法观察
短句来源
     LOCALIZATION OF THE NEURONS CONTAINING CALBINDIN-D_(28K) mRNA IN THE RAT BRAINSTEM
     含Calbindin-D28K mRNA的神经元在大鼠脑干中的分布—原位杂交组织化学法观察
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  hybridization histochemistry
Immunohistochemistry andin situ hybridization histochemistry (ISHH) were used to detect the expression of bcl-2 and bax genes.
      
The sections of kidneys were examined by an electric microscope, PAS staining, immunohistochemical staining and in situ hybridization histochemistry.
      
The expressions of c-fos/c-jun in cultured rat hippocampus cells (1 d, 3 d, 5 d, 7 d, and 10 d) were studied by using both in situ hybridization histochemistry and SABC immunohistochemistry techniques.
      
Testicular expression of both porf-1 and -2 was analyzed as a function of maturational stage, aging and hypophysectomy by the solution hybridization/nuclease protection assay, and cellular location determined byin situ hybridization histochemistry.
      
VP mRNA in the paraventricular and supraoptic nuclei (PVN and SON) and corticotrophin-releasing factor (CRF) mRNA in the PVN were measured by in situ hybridization histochemistry.
      
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  hybridization combined
Fluorescence in situ hybridization combined with immunofluorescent staining for rapid detectionof Nmyc amplification in neurobla
      
By using in situ hybridization combined with compute physiological quantitative imaging analysis techniques, the influence of intrinsic NO on the expression of IL-4 mRNA and IFN-γ mRNA in the airway inflammatory cells was observed.
      
Transfer of resistance against Phoma lingam to Brassica napus by asymmetric somatic hybridization combined with toxin selection
      
Southern hybridization combined with pulsed-field gel electrophoresis revealed that CmFAD9 is probably located on chromosome XI of the 17 C.
      
In situ hybridization combined with immunohistochemical techniques has been applied to study patients affected by mitochondrial myopathies with large mitochondrial (mt)DNA deletions.
      
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Using in situ hybridization histochemistry, we examined the localization of the neurons containing calbindin-D28K mRNA in the rat forebrain. Regions with strong labeling by the probe were as follows: the anterior olfactory nucleus, the cerebral cortex, the caudate-putamen, the accumbens, the habenular complex, the hypothalamus, the dentate gyrus and some nuclei of the thalamus and amygdala. However, some other regions such as the olfactory bulb, the tenia tecta,the medial geniculate nucleus, and some nuclei...

Using in situ hybridization histochemistry, we examined the localization of the neurons containing calbindin-D28K mRNA in the rat forebrain. Regions with strong labeling by the probe were as follows: the anterior olfactory nucleus, the cerebral cortex, the caudate-putamen, the accumbens, the habenular complex, the hypothalamus, the dentate gyrus and some nuclei of the thalamus and amygdala. However, some other regions such as the olfactory bulb, the tenia tecta,the medial geniculate nucleus, and some nuclei of the thalamus and amygdala contain the neurons labeled moderately. The neurons in the olfactory tubercle, the septum, the nucleus of the diagonal band and the lateral geniculate nucleus were weakly labeled. Therefore, the transcripts of calbindin-D28K gene show a region-specific localization in rat forebrain, indicating a important role of calbindin-D28K in these regions.

本实验应用原位杂交组织化学技术,利用同位素标记的寡核苷酸探针,对大鼠前脑含Calbindin-D28 K mRNA的神经元的分布状况进行了详细的观察。结果发现在不同脑区或核团中,标记神经元的数量和标记强度各不相同。某些部位含许多强阳性神经元,如:前嗅核、大脑皮质、尾壳核、缰核、下丘脑、齿状回及中脑和杏仁复合体中的部分核团;然而,在另外一些脑区中,标记细胞呈中等阳性,如:嗅球的球旁细胞、盖带、梨状区内核、海马的CA1区中的锥体细胞层以及丘脑和杏仁核复合体中的部分核团。少数脑区中的标记细胞呈弱阳性,且数量较少,如;嗅结节、隔区、斜角带核等。这些结果表明含Calbindin-D28K mRNA的神经元在大鼠前脑中具有区域特异性分布特点,从而提示Calbindin-D28K在神经系统中的某些部位可能具有重要的作用。

The combination of in situ hybridization histochemistry with immunocytochemistry was studied to demonstrate somatostatin immunoreactivity and somatostatin mRNA on the same section of the rat hypothalamus.A comparison was made to assess the effects of 6 fixatives on in situ hybridization histochemistry and its comination with immunohistochemistry.The results showed that Zamboni's fixative was the best for the combination of in situ hybridization histochemistry with immunohistochemistry while 4% paraformaldehyde-phosphate...

The combination of in situ hybridization histochemistry with immunocytochemistry was studied to demonstrate somatostatin immunoreactivity and somatostatin mRNA on the same section of the rat hypothalamus.A comparison was made to assess the effects of 6 fixatives on in situ hybridization histochemistry and its comination with immunohistochemistry.The results showed that Zamboni's fixative was the best for the combination of in situ hybridization histochemistry with immunohistochemistry while 4% paraformaldehyde-phosphate buffer was best for in situ hybridization histochemistry.All,somatostatin immunoreactive neurons demonstrated by combining in situ hybridization histochemistry with immunohistochemistry contained silver granules of somatostatin mRNA in the periventricular nucleus of the rat hypothalamus.The amount of somatostatin gene transcription in proportional that of somatostatin translation from its mRNA.

介绍一种用原位杂交组织化学与免疫组织化学结合法在同一切片中显示生长抑素mRNA和相对应的生长抑素.同时还比较了六种不同固定液对原位杂交组织化学和原位杂交组织化学与免疫组织化学结合法的影响.结果表明Zamboni氏固定液用于原位杂交组织化学与免疫组织化学结合法最佳,而4%多聚甲醛磷酸缓冲液用单纯原位杂交最佳.原位杂交组织化学与免疫组织化学结合法显示的下丘脑室周核生长抑素免疫反应阳性神经元均含有相应生长抑素mRNA,而且多数神经元生长抑素基因的转录量与生长抑素的翻译量成正比.

Using in situ hybridization histochemistry, we examined the localization of the neurons containing calbindin-D_(28K) mRNA in the rat brainstem. In some regions, many neurons were strongly labeled, such as: the cerebellar cortex, the nucleus of lateral lemniseus, the nucleus of trapezoid body) the inferior olivary nucleus and the parabrachial nucleus, However, other regions such as the interpeduncular nucleus, the substantia nigra (compact part), the cochlear nucleus, and the area postrema contained neurons labeled...

Using in situ hybridization histochemistry, we examined the localization of the neurons containing calbindin-D_(28K) mRNA in the rat brainstem. In some regions, many neurons were strongly labeled, such as: the cerebellar cortex, the nucleus of lateral lemniseus, the nucleus of trapezoid body) the inferior olivary nucleus and the parabrachial nucleus, However, other regions such as the interpeduncular nucleus, the substantia nigra (compact part), the cochlear nucleus, and the area postrema contained neurons labeled moderately. The regions containing weakly labeled neurons were as follows: the superior colliculus, the pretectal region, the red nucleus, the principal and the spinal nuclei of trigeminal nerve, the gracileand cuneate nuclei, the inferior collicultis, the vestibular nuclei, the nucleus of the solitary- tract and some nuclei of the reticular formation. Therefore, the neurons containing calbindin-D_(28K) mRNA showed a regionspecific distribution in rat brainstem, suggesting an important role of calbindin-D_(28K) in some brain functions.

用原位杂交组织化学技术,对含Calbindin-D28K mRNA的神经元在大鼠脑干中的分布进行全了面观察。发现不同脑区或核团中所含阳性神经元的数量及其标记强度各不相同。其中含强阳性杂交信号的区域有:小脑皮质、外侧丘系核、斜方体核、下橄榄核及臂旁核;含中等强度杂交信号的区域包括:脚间核、黑质致密部、耳蜗核和最后区。而在其它大部分脑区中杂交信号呈弱阳性,如:上丘、顶盖前区、红核、三叉神经感觉核簇、后索核、下丘、前庭核群、孤束核、中央灰质和网状结构中的部分核团。含Calbindin-D28KmRNA的神经元在脑干中的这种区域特异性分布特点提示在神经系统的某些生理功能中,Calbindin-D28K可能起重要的作用。

 
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