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肾细胞系
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  kidney cell line
     Metheods The cellular membrane of the gastric carcinoma cell line BGC-823 and human normal kidney cell line 293 was made. Each of them was analyzed by radioactive ligand of LHRH-PE40 marked by 125I and their competitive binding with LHRH was detected.
     方法将胃癌细胞系BGC-823及正常人肾细胞系293制备成细胞膜,利用125I标记的LHRH-PE40与两种细胞膜进行放射性配基分析, 以及与LHRH竞争结合分析。
短句来源
     A persistent infection of hepatitis A virus (HAV) strain HM-175 was established in a fetal rhesus monkey kidney cell line (Frhk-4).
     用甲型肝炎病毒(HAV)HM175株感染恒河猴胚肾细胞系(Frhk-4),经过连续传代培养,建立了一株持续感染HAV的细胞株。
短句来源
     to observe the function state of the mutant C-kit gene in GIST, the recombinant eukaryotic expression vector plasmids with mutant C-kit cDNA was constructed and stably transfected into human embryonic kidney cell line(293 cell line), and the effect of the recombinant plasmids to the cell proliferation and cell cycle was detected;
     构建含突变的C-kit基因cDNA真核表达载体,将其转染人类胚胎肾细胞系,通过观察突变型C-kit基因对细胞增殖及细胞周期的影响,分析其功能状态;
短句来源
     And, as human embryonic kidney cell line, HEK293 cells has become an appropriate system to produce both gene therapy vectors and therapeutic proteins, the work presented herein focused in CFIC of HEK293 cells.
     鉴于人胚肾细胞系HEK293细胞已被广泛地用作生产基因治疗载体和重组蛋白的哺乳动物细胞,本论文研究围绕HEK293细胞的无载体固定化培养展开。
短句来源
     Objective To assess the infectivity of porcine endogenous retrovirus (PERV) via in vitro infection of human embryonic kidney cell line HEK-293. Methods PERV particles were detected by immunoelectron microscopy.
     目的 对猪内源性逆转录病毒(PERV)体外感染人胚肾细胞系HEK 293的能力进行检测。
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  “肾细胞系”译为未确定词的双语例句
     We constructed the eukaryotic expression vector pcDNA3GFP for determination of expression of green fluorescent protein (GFP) .
     拟构建绿色荧光蛋白(green fluorescent protein, GFP)基因的真核表达载体 pcDNA_3GFP,并观察其在 MDCK细胞(大肾细胞系)中的表达。
短句来源
     Methods The WHc fragment in plasmid pwCTLA-4-WHc was replaced with HBc fragment to construct plasmid pwCTLA-4-HBc. The fusion protein expression in pwCTLA-4-HBc transfected BHK and Hela cells was detected by indirect immunofluorescence assay and chemiluminescent microparticle immunoassay respectively.
     方法通过限制性酶切和T4连接,以HBc基因片段置换pwCTLA-4-WHc质粒中的WHc片段,得到质粒pwCTLA-4-HBc,分别转染幼仓鼠肾细胞系(BHK)和人宫颈癌细胞系(Hela),间接免疫荧光和化学发光免疫法检测融合蛋白的表达。
短句来源
     HEK293 cell was chose to study the kidney damage of cadmium and to explore the significance of caspase 3,Bcl-2 and AIF(apoptosis inducing factor)in the apoptosis of cells in- duced by cadmium.
     本课题研究了氯化镉(CdCl_2)诱导HEK293细胞(人胚胎肾细胞系)的凋亡,初步探讨了凋亡过程中Caspase-3、Bcl-2的变化和凋亡诱导因子(AIF)的转移以及它们的意义。
短句来源
     Studies on grass carp hemorrhage virus (GCHV) genome transcription in vivo have been carried out Viral mRNA labeled with α-~(32)p ATP was extracted from infected cells treated with actinomycin D and detected by means of liquid phase hybridization and Northern blot hybridization.
     本文采用α-[~(32)p]ATP标记物在鱼肾细胞系(CIK)系统中,对草鱼出血病病毒(Grass carp hemorrhage virus,GCHV)基因组进行了体内转录的研究。 通过放线菌素D抑制宿主细胞基因组的转录活动,从感染病毒细胞中分离出病毒的mRNA,分别采用液相杂交和Nortbern blot方法检查病毒mRNA的转录情况。
短句来源
     The antiviral activity of rPoIFNβ was tested by CPE_(50) method. The results showed that the rPoIFNβ was of high antiviral activity,which was 2.0×106 U/mg against VSV in MDBK cells.
     以细胞病变抑制法(CPE50)测定干扰素抗病毒活性,在MDBK(牛肾细胞系)中表达的猪β-干扰素的抗VSV比活性达到2.0×106U/mg;
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  相似匹配句对
     The Subtypes of Renal Cell Carcinoma
     细胞癌亚型
短句来源
     IL-6 expression in the renal and bladder carcinoma cell lines
     白细胞介素6在癌和膀胱癌细胞中的表达
短句来源
     Ultrastructural typing of renal cell carcinoma (RCC)
     细胞癌的超微结构分型
短句来源
     Establishment and characterization of a human renal carcinoma cell line RCC-9863
     人透明细胞细胞RCC-9863的建立及其生物学特性
短句来源
     Diagnostic evaluation of MR urography in renal pelvis and ureter transitional cell carcinoma
     MR泌尿造影对和输尿管移行细胞癌的诊断
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  kidney cell line
Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293
      
In embryonic kidney cell line 293 the enzyme was excluded from the nuclei and distributed in fractions of soluble cytosolic proteins and low-density microsomes.
      
Recombinant NP was synthesized in Escherichia coliand in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase.
      
The genes that were induced and suppressed in human embryonic kidney cell line RH upon the infection with tick-borne encephalitis virus were studied by the method of subtractive hybridization.
      
Their cotransfection into the human embryonic kidney cell line HEK293T provided the production of a full-size recombinant human antibody.
      
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Essential -media tested were Earle, Earle +0.5% lactalbumin hydrolysate (Oxoid) and RPMI-1640. Baby jird testis and kidney tissue cell lines were used for culture. The most: favourable condition for the maintenance of the larvae in vitro was in a range of pH 6.4-7.5 and at temperatures of 36.5-37.5℃. The baby jird testis cell in growth medium RPMI-1640 was the best cell line tested with increases of 32.6% of the length and 36.8% of the width of the larvae taking place in 38 days cultivation. We observed successful...

Essential -media tested were Earle, Earle +0.5% lactalbumin hydrolysate (Oxoid) and RPMI-1640. Baby jird testis and kidney tissue cell lines were used for culture. The most: favourable condition for the maintenance of the larvae in vitro was in a range of pH 6.4-7.5 and at temperatures of 36.5-37.5℃. The baby jird testis cell in growth medium RPMI-1640 was the best cell line tested with increases of 32.6% of the length and 36.8% of the width of the larvae taking place in 38 days cultivation. We observed successful moulting in both MT and MK cell lines. Brugia malayi began to moult on the tenth day. The survival of the larvae maintained on MK cell and MT cell were prolonged to 23 and 38 days respectively.

马来丝虫感染期幼虫(L_3)在沙鼠睾丸细胞系培养液中发育良好,一般在10~14天开始蜕皮进入第四期幼虫(L_4),成双地扭集在睾丸细胞系中最长可活38天,幼虫长度增加32.6%,宽度增加36.8%,可初步分辨雌雄;在鼠肾细胞系培养液中L_3于10~20天开始蜕皮,少数进入L_4,最长可活23天,幼虫长度增加25.9%,宽度增加34.5%,L_3在单纯Earle、Earle加水解乳蛋白及RPMI-1640中,仅能活2~4天。在培养过程中应防止细菌污染,合适的温度和pH亦系培养成功的重要因素。

The susceptibility to grass carp reovirus (GCRV) of CAB-80, BCC, PHG, GCE GCTF-2 and GCK-84 (these cell lines were derived from crucian carp blastula embryos, herbivorous bream caudal fins, loach haploid gastrula embryos, grass carp gastrula embryos, grass carp caudal fins and grass carp kidney, respectively), as well as four clones isolated from GCK-84, were analyzed comparatively. Sensitivity of various degrees was confirmed in the infected cell lines (except PHG) among which GCK-84 was the most sensitive....

The susceptibility to grass carp reovirus (GCRV) of CAB-80, BCC, PHG, GCE GCTF-2 and GCK-84 (these cell lines were derived from crucian carp blastula embryos, herbivorous bream caudal fins, loach haploid gastrula embryos, grass carp gastrula embryos, grass carp caudal fins and grass carp kidney, respectively), as well as four clones isolated from GCK-84, were analyzed comparatively. Sensitivity of various degrees was confirmed in the infected cell lines (except PHG) among which GCK-84 was the most sensitive. It is demonstrated that GCRV, in vitro, has no strict species specificity. Typical symptom of muscular hemorrhage could be replicated when fingerlings of grass carp were infected with the virus which had been propagated in GCK-84. The virus produced by GCK-84, GCRF-2, CAB-80, BCC and PHG was titred in GCK-84 cells. The titers (TCID50/ml) were 8.24, 7.36, 2.90, 2.15 and 1.33 respectively. Sicells. difference was also observed in the four clones and GCK-84, with the titers ranging fro6.3 to 9.32. The results prescnted above predict the relative potential significance in the disease-resistant breeding of fish cell engineering.Serious damages in GCRV-infected GCK-84 cells could be seen with electron microscopy. The observed virus was in the shape of spherical particles having a size of 58 nm in diameter and a core with high electronic density approximately 38 nm in diameter on the average. The distribution of virions in cells could be divided into three patterns: existing individually in cytoplasm, congregating in large crystalline aggregates bound by membranceous structures, gathering together in an orderly or disorderly arrangement without a surrounding membrane.

比较研究了鲫鱼异倍体细胞系(CAB-80)、团头鲂尾鳍细胞系(BCC)、大鳞副泥鳅雌核发育单倍体胚胎细胞系(PHG)、草鱼胚胎细胞系(GCE)、草鱼尾鳍细胞系(GCRF-2)、草鱼肾细胞系(GCK-84)及其四个克隆对草鱼呼肠孤病毒(GCRV)的敏感性。证实了这些细胞(PHG除外)在不同程度上对GCRV敏感,其中以GCK-84的敏感性最强。这表明,在体外培养条件下,GCRV并无严格的种族特异性。用经GCK-84传代的病毒感染草鱼种,能复制出典型的出血病症状。用GCK-84检测了病毒在GCK-84、GCRF-2、CAB-80、BCC和PHG中的滴度(TCID_(50/ml)),其值分别为8.24,7.36,2.90,2.15和1.33。4个克隆与肾细胞系对病毒的敏感程度亦不尽相同,其滴度在6.3到9.32之间变化。上述结果对细胞工程抗病育种预示有较大的潜在意义。在电镜下可见GCRV对被感染的细胞造成了严重的破坏。病毒为平均直径58nm的球形颗粒,具有一个高度电子密度的核心,平均直径约为38nm。病毒在细胞中的分布方式有三种:即散布于细胞质中的、呈晶格状包于一膜状结构中的和整齐或不整齐地聚...

比较研究了鲫鱼异倍体细胞系(CAB-80)、团头鲂尾鳍细胞系(BCC)、大鳞副泥鳅雌核发育单倍体胚胎细胞系(PHG)、草鱼胚胎细胞系(GCE)、草鱼尾鳍细胞系(GCRF-2)、草鱼肾细胞系(GCK-84)及其四个克隆对草鱼呼肠孤病毒(GCRV)的敏感性。证实了这些细胞(PHG除外)在不同程度上对GCRV敏感,其中以GCK-84的敏感性最强。这表明,在体外培养条件下,GCRV并无严格的种族特异性。用经GCK-84传代的病毒感染草鱼种,能复制出典型的出血病症状。用GCK-84检测了病毒在GCK-84、GCRF-2、CAB-80、BCC和PHG中的滴度(TCID_(50/ml)),其值分别为8.24,7.36,2.90,2.15和1.33。4个克隆与肾细胞系对病毒的敏感程度亦不尽相同,其滴度在6.3到9.32之间变化。上述结果对细胞工程抗病育种预示有较大的潜在意义。在电镜下可见GCRV对被感染的细胞造成了严重的破坏。病毒为平均直径58nm的球形颗粒,具有一个高度电子密度的核心,平均直径约为38nm。病毒在细胞中的分布方式有三种:即散布于细胞质中的、呈晶格状包于一膜状结构中的和整齐或不整齐地聚集在一起但无膜包裹的。

This paper reported on improved in vitro cultute system of human lymphatic filarial larvae. Four culture systems were used. Third-stage larvae of Brugia malayi were best maintained, developing and molted twice in the medium containing modified RPMI-1640 medium, supplemented with 20% newborn calf serum and fauman embrsyonic kidney cell line as feeder layer. This culture system kept larvae alive up to 54 days. Brugia malayl third-stage larvae began to moult on the 8-10th day and again on the 32-36th day; Wuchereria...

This paper reported on improved in vitro cultute system of human lymphatic filarial larvae. Four culture systems were used. Third-stage larvae of Brugia malayi were best maintained, developing and molted twice in the medium containing modified RPMI-1640 medium, supplemented with 20% newborn calf serum and fauman embrsyonic kidney cell line as feeder layer. This culture system kept larvae alive up to 54 days. Brugia malayl third-stage larvae began to moult on the 8-10th day and again on the 32-36th day; Wuchereria bancrofti third stage larvae grew and developed to the fourth-stage and juvenile and survived to 57 days. They began to moult on the 12-18th days and again on the 32-44th day. This culture system was thought to be useful for studies on morphology and sensitivity to drugs.We also studied several cell-free culture systems. Among them, Best survival, growth and devclopment were obtained in 1:1 mixture of modified RPMI-1640 and TC199 medium supplemented with 20% newborn calf serum. Both Wuchereria bancrofti. and Brugia malayi third-stage larvae grew and developed to the fourth stage larvae and juveniles and survived to 36 days and 42 days respectively. The availability of such culture systems for human filariasis would facilitate studies of biochemistry, immunology, duction of moncclonal antibodies and vaccine.

在4种培养系统中,马来丝虫Ⅲ期幼虫在改良RPMI1640、20%小牛血清和人胚肾细胞系为饲养层的培养系统中生长发育良好,可以从Ⅲ期幼虫蜕皮2次发育到童虫,最长存活时间为54d。在单纯改良RPMI1640、TC199和20%小牛血清中培养,从Ⅲ期幼虫发育到童虫的最长存活时间为42d。班氏丝虫Ⅲ期幼虫在上述2种培养系统中,分别存活57和36d,从Ⅲ期幼虫蜕皮进入Ⅳ期幼虫和童虫。对马来丝虫幼虫体外培养蜕皮时间、虫体大小与沙鼠体内发育情况进行了比较。

 
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