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   线性化病毒 的翻译结果: 查询用时:0.194秒
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线性化病毒
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  linear virus
     PF4 gene was inserted in Bombyx mori baculovirus transfer vector pBacPAK8 and cotransfected with linear virus Bm BacPAK6 into BmN cells. The homologous recombination occurred inside the cells then the recombinant virus BacPAK PF4 was obtained.
     将人血小板因子Ⅳ (HumanPlateletFactorⅣ ,简称hPF4)基因重组于家蚕杆状病毒转移载体pBacPAK8中 ,获得重组转移载体pBacPAK PF4,并与线性化病毒Bm BacPAK6DNA共转染家蚕培养细胞 ,在细胞内发生同源重组 ,获得重组病毒BacPAK PF4。
短句来源
     Six×his-hGM-CSF fusion gene was inserted in silkworm baculovirus transfer vector pBacPAK8 to construct the recombinant transfer vector pBacPAKHis-GM-CSF. The recombinant transfer vector DNA and linear virus Bm-BacPAK6 DNA were co-transfected into BmN cells. The homologous recombination occurred inside the cells, so that the recombinant virus vBacPAKHis-GM-CSF was obtained.
     将融合6个组氨酸(6×his)序列的hGM-CSF基因插入杆状病毒转移载体pBacPAK8中得到杆状病毒重组转移载体pBacPAKHis-GM-CSF,pBacPAKHis-GM-CSF DNA与线性化病毒Bm-BacPAK6 DNA共转染BmN细胞,获得了表达rhGM-CSF融合蛋白的重组病毒vBacPAKHis-GM-CSF.
短句来源
  “线性化病毒”译为未确定词的双语例句
     2. The pPGH030 and the linear Bm-BacPAK6 were co-transfected into the cell line of Bombyx mori (BmN), then the recombinant virus, Bm-BacPAK6-pgh was obtained successfully.
     2.将pPGH030 与线性化病毒Bm-BacPAK6 共转染家蚕细胞(BmN),成功获得了猪生长激素重组杆状病毒Bm-BacPAK6-pgh。
短句来源
     The cDNA of pGH without signal peptides was reconstructed by PCR, then recombined with the vector pBacPAK-His1.The recombined plasmid,pPGH030,and linear Bm-BacPAK6 co-transfacted in the cell line of Bombyx mori (BmN),then the recombinant virus, Bm-BacPAK6-pgh was obtained successfully.
     将通过PCR技术改建并去除了信号肽的猪生长激素cDNA基因克隆入转移载体pBacPAK His1,构建了重组转移载体pPGH0 30 ,进而将pPGH0 30与线性化病毒Bm BacPAK6共转染BmN细胞 ,成功获得了猪生长激素重组杆状病毒Bm BacPAK6 pgh。
短句来源
     Angiostatin (Kl-3 ) gene was inserted into Bombyx mori baculovims transfer vector pBacPAKS and cotransfected with lineared DNA of Bm-BacPAK6 virus into BmN cells.
     本研究首先将人血管抑素(angiostatin)基因重组于家蚕杆状病毒转移载体pBacPAK8中,获得重组转移载体pBacPAK-angiostatin,并与线性化病毒Bm-BacPAK6 DNA共转染家蚕细胞株,获得重组病毒BacPAK-angiostafin。
短句来源
     In order to increasing hIL-4 expression in Bombyx mori baculovirus expression vector system, The hIL-4 gene (460bp) was inserted into Bombyx mori baculovirus transfer vector pBacPAO and co-transfected with linearized DNA of Bm-BacPAK6 virus into BmN cells.
     将460bp的目的基因插入家蚕杆状病毒转移载体pBacPAK8中,构建成重组载体pBacPAK-hlL-4,与线性化病毒Bm-BacPAK6共转染家蚕BmN细胞,获得重组病毒BacPAK-hIL-4。
短句来源
     The transfer vectors containing the chimeric genes were contransferted into Sf9 cells with linerized virus DNA and recombinant virus were isolated by dot hybridization PCR and southern blot analysis.
     含嵌合抗体基因的转移载体与线性化病毒DNA共转染Sf_9细胞,并通过点杂交、PCR扩增和Southern blot分析获得重组病毒.
短句来源
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  相似匹配句对
     sendai virus
     仙台病毒
短句来源
     killing viruses;
     杀灭病毒 ;
短句来源
     Linearization of the GM Counter
     GM计数管的线性化
短句来源
     Cascade Linearized Optical Modulator
     级联线性化光调制器
短句来源
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  linear virus
Only linear virus DNA was detected in all the untreated, in vitro-infected nuclei.
      


Both the heavy and light chain of a murine human chimeric antibody with specificity for hepatitis B virus surface antigen have been expressed separately in a baculovirus expression system.The heavy and light chain variable region (VH and VL) genes encoding the murine monoclonal antibody OH3 were combined with human γ3 and κ constant region genes to construct the murine human chimeric genes,respectively.The transfer vectors containing the chimeric genes were cotransfected into Sf9 cells with linearized...

Both the heavy and light chain of a murine human chimeric antibody with specificity for hepatitis B virus surface antigen have been expressed separately in a baculovirus expression system.The heavy and light chain variable region (VH and VL) genes encoding the murine monoclonal antibody OH3 were combined with human γ3 and κ constant region genes to construct the murine human chimeric genes,respectively.The transfer vectors containing the chimeric genes were cotransfected into Sf9 cells with linearized virus DNA and recombinant viruses were isolated by dot hybridization,PCR and Southern blot analysis.The chimeric heavy and light chain was expressed respectively in the recombinant viruses infected insect cells and the characterization by Western blot and competitive ELISA demonstrated that both of the expressed chimeric heavy and light chain have the specificity of HBsAg binding.

应用杆状病毒表达系统在昆虫细胞中表达了抗乙肝病毒表面抗原(HBsAg)人-鼠嵌合抗体重、轻链基因。鼠源单克隆抗体OH3重、轻链可变区(VH、VL)cDNA分别与人免疫球蛋白恒区γ3、κcDNA拼接成人-鼠嵌合抗体基因。含嵌合抗体基因的转移载体与线性化病毒DNA共转染Sf9细胞,并通过点杂交、PCR扩增和Southernblot分析获得重组病毒。Westernblot和竞争ELISA表明以重组病毒感染的昆虫细胞分别表达了嵌合重链和轻链,并证实表达的嵌合重链和轻链兼有结合HBsAg的特性。

The heavy and light chain genes of a murine-human chimeric antibody with specificity for carcinoembryonic antigen have been expressed separately in a baculovirus expression system.The genes encoding the heavy and light chain variable region (VH and Vk) were combined to the human Cr3 and Ck genes respectively to construct the murine-human chimeric antibody genes. The transfer vectors containing the chimeric genes were contransferted into Sf9 cells with linerized virus DNA and recombinant virus were isolated by...

The heavy and light chain genes of a murine-human chimeric antibody with specificity for carcinoembryonic antigen have been expressed separately in a baculovirus expression system.The genes encoding the heavy and light chain variable region (VH and Vk) were combined to the human Cr3 and Ck genes respectively to construct the murine-human chimeric antibody genes. The transfer vectors containing the chimeric genes were contransferted into Sf9 cells with linerized virus DNA and recombinant virus were isolated by dot hybridization PCR and southern blot analysis. Western blot showed the chimeric heavy and light chain were expressed in the recombinant virus infected Sf9 cells. RIA and ELISA demonstrated that both of the expressed chimeric heavy and light chain have the CEA-binding abilities.

本文采用杆状病毒表达系统在昆虫细胞(Sf_9,秋粘虫细胞)中表达了抗癌胚抗原(CEA)鼠-人嵌合抗体重、轻链基因.将鼠源性单抗杂交瘤细胞中已克隆到的重、轻链可变区(V_H、Vk)基因分别与人免疫球蛋白恒定区基因Cr_3、Ck相拼接,构建鼠人嵌合抗体基因.含嵌合抗体基因的转移载体与线性化病毒DNA共转染Sf_9细胞,并通过点杂交、PCR扩增和Southern blot分析获得重组病毒.Western blot分析表明以重组病毒感染的Sf_9细胞分别表达了嵌合重链和轻链,放射免疫分析法(RIA)和间接ELISA测定的结果表明嵌合重、轻链基因表达产物具有与CEA结合的能力.

Aim : TostudytheexpressionofhBMP2inthebaculovirussystem .Methods:ThecDNAencodingthewhole lengthofhumanbonemorphogeneticprotein 2 (hBMP2 )wasclonedintothetransfervectorPK8.Therecombinantvector containighBMP2cDNAwasidentifiedbyrestrictionenzyme.ThelinearizedvirusDNAandtheaboverecombinantvector complexwastransfectedintoSf 9cellsbylipofectin .TherecombinantviruswasidentifiedbyLacZblue -whiteselection . PCRanalysisofvirusDNAwasdone.Immunofluorescenceandelectromicroscopicanalyseswerealsoperformed .Results:The...

Aim : TostudytheexpressionofhBMP2inthebaculovirussystem .Methods:ThecDNAencodingthewhole lengthofhumanbonemorphogeneticprotein 2 (hBMP2 )wasclonedintothetransfervectorPK8.Therecombinantvector containighBMP2cDNAwasidentifiedbyrestrictionenzyme.ThelinearizedvirusDNAandtheaboverecombinantvector complexwastransfectedintoSf 9cellsbylipofectin .TherecombinantviruswasidentifiedbyLacZblue -whiteselection . PCRanalysisofvirusDNAwasdone.Immunofluorescenceandelectromicroscopicanalyseswerealsoperformed .Results:The targetDNAfragmentwasfoundafterrestrictionenzymedigestionandwasconfirmedbyPCRanalysis.Thepositivegranaules weredistributedinthecytoplasmandonmembrane .Conclusion :hBMP2isfoundtobeexpressedinthissystem .

目的:研究hBMP2在昆虫杆状病毒系统中的表达规律。方法:将编码hBMP2的全长cDNA克隆入转移载体PK8中,重组后的载体与线性化的病毒DNA经脂质体包裹转染昆虫细胞。重组病毒经蓝白筛选后,提取病毒DNA进行PCR扩增,鉴定目的基因。收集重组病毒感染4d的细胞,进行免疫荧光及电子显微镜观察。结果:含有目的基因的重组载体经酶切可切下相应大小的目的基因片段。PCR产物的电泳结果表明,有目的基因片段的扩增。免疫荧光染色呈阳性的颗粒分布在昆虫细胞的胞浆及胞膜。结论:初步证实,hBMP2在此套表达系统中获得了表达。

 
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