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正确阅读
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  right reading
     The recombinant plasmid that has right reading frame was transformed into competent cell of BL 21 (DE 3) and then induced to express by IPTG at 37℃. The merged protein′s molecular weight is approximated to 29 000 with the expression weight of 11%.
     该重组质粒经酶切鉴定后 ,将其具有正确阅读框架的重组质粒转化到大肠杆菌 BL2 1 (DE3)感受态细胞 ,37℃下经 IPTG诱导表达 ,得到相对分子质量约 2 90 0 0的融合蛋白 ,表达量约为 11%。
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  “正确阅读”译为未确定词的双语例句
     4. DNA sequencing confirmed that the recombinant eukaryotic expression plasmid (pcDNA3.1-BLG-HNP-1) containing BLG and HNP-1 had been constructed correctly.
     4.测序结果表明,该质粒上的BLG和HNP-1的基因序列无突变,连接方向正确,阅读框无移位,pcDNA3.1-BLG-HNP-1表达质粒构建成功。
短句来源
     Improve Reading Ability in English through Grasping Correct Reading Methods
     掌握正确阅读方法提高英语阅读能力
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     3.Utilizing vector pET-22b(+) to construct expression vector pET-chiB, through validating and analyzing, the result indicated that the cloned chiB gene had been inserted the correct reading frame of expression vector;
     3. 利用载体 pET-22b(+),构建 chiB 表达载体 pET-chiB,通过验证分析表明,所克隆的基因 chiB 已置于表达载体的正确阅读框架下;
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     RESULTS:①The expression vector pGEX-4T-ADAM28 was constructed successfully. The coding region sequence of ADAM28 was correctly inserted into the expression vector by endonucleases digestion,PCR identification and DNA sequencing analysis. Its open reading frame was integra.
     结果:①成功构建融合表达载体pGEX-4T-ADAM28,并经酶切鉴定、PCR鉴定和测序验证显示插入的ADAM28序列正确,阅读框架完整,未发现突变。
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     Then took the PEA recA downstream of T 7 promoter in pET 17bplasmid to construct PEA recA expression plasmid identified by endonuclease enzymes digesting and PCR,the PEA recA gene was downstream of T 7 promoter.
     融合基因经BamHI及EcoRI酶切,以正确阅读框架插入带有T7表达启动子的质粒pET-17b,构建了可表达PEA-recA融合基因的质粒pERA-17b。 经酶切分析及PCR扩增检测证明,绿脓杆菌recA已插入PEA毒性基因中。
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  相似匹配句对
     READING
     阅读
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     Teach students the correct method of reading;
     教给学生正确阅读方法;
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     How to Read and Understand the Official Document
     怎样正确阅读和理解公文
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     Reading Music
     耳朵的阅读
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     design, such as reasonable shape selection,correct calculation, reliable construction and safe mooring system.
     正确的计算;
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查询“正确阅读”译词为用户自定义的双语例句

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  right reading
As long as it is interesting to the student and at the right reading level, you can use it.
      
Texts of the right reading level are neither too easy nor too hard for a particular reader.
      
Use Mirror Image function to ensure a right reading image.
      


Enlightens us that the key to improvement in English speed reading capacity owes to cultivation of appropriate reading habits, mastery of speed reading skills and practising time-keeping readings.

探讨了英语快速阅读原则,认为提高快速阅读能力的关键在于养成正确的阅读习惯,掌握快速阅读的技能,并进行计时阅读实践。

Using recombinant plasmid pBST2-6 containing enteriotoxigenicEscherichia coli heat-stable

利用含大肠杆菌耐热性肠毒素ST1基因的质粒pBST2-6和含LacZ基因(编码β-半乳糖苷酶)的载体pUC18,构建成ST1-LacZ融合基因。将质粒pBST2-6用限制性内切酶BamHI和BglⅡ消化,经3%琼脂糖凝胶电泳分离、透析袋电洗脱法回收147bpDNA片段,再将载体pUC18用BamHI消化、碱性磷酸酶处理,最后将处理好的pUC18DNA与147bpST1DNA通过T4DNA连接酶进行粘性末端连接,转化至受体菌DH5a中。通过菌落原位杂交筛选,共筛选出53个为ST1基因探针杂交阳性的菌落。菌落原位杂交阳性的菌株经培养和抽提纯化质粒后,经限制性内切酶(EcoRI/XbaI和EcoRI/BamHI)酶切分析和DNA序列分析,证明重组质粒pXST1含有ST1基因,而且ST1基因在重组DNA中连接方向是正确的,具有正确的阅读框架。再者,DH5a(pXST1)菌株能在含x-Gal的LB平板上长成蓝色菌落,表明该菌株能表达具有β-半乳糖苷酶活性的大肠杆菌耐热性肠毒素ST1-β-半乳糖苷酶融合蛋白。ST1-LacZ融合基因的构建,为研究ST的免疫原性和抗ST抗体在预防产肠毒素性大肠杆菌(ETEC)感染中的作用?

The 5'terminus of the gene that codes for the heat-stable enterotoxin I of Escherichia colt (T1) was genetically fused to the 3'terminus of the LacZ gene that codes for the β-galactosidase. We constructed the recombinant plasmid pXST1 and studied it in detail by DNA sequencing. The results showed that the pXST1 carried two T1 genes,had positive reading frame, and coule express the T1-β-glactosidase fusion protein. More importantly, the fusion protein was nontoxic and immungenic. BALB/c mice imunized with crude...

The 5'terminus of the gene that codes for the heat-stable enterotoxin I of Escherichia colt (T1) was genetically fused to the 3'terminus of the LacZ gene that codes for the β-galactosidase. We constructed the recombinant plasmid pXST1 and studied it in detail by DNA sequencing. The results showed that the pXST1 carried two T1 genes,had positive reading frame, and coule express the T1-β-glactosidase fusion protein. More importantly, the fusion protein was nontoxic and immungenic. BALB/c mice imunized with crude prepation containing the fusion protein produced antibodies that was recognize T1 enterotoxin in vitro,ignificantly. These sera antibodies were able to neutralize the biological activity of native T1 enterotoxin in the suckling mouse assay. Hence the recombinant strain DH5α(pXST1 )can be used candidate of vaccine strain.

对已构建的重组质粒pXST1(含有ST1和LacZ基因)进行了核苷酸序列分析和免疫原性研究。序列分析结果表明,该质粒中含有2个正向串联在一起的ST1基因,且融合在LacZ基因的上游,具有正确的阅读框架。免疫实验结果表明,构建的DH5α(PXST1)重组菌株安全无毒。该重组菌株表达的ST1融合蛋白能够诱发BALB/c鼠产生抗体,且抗体能中和天然ST1肠毒素活性,具有较好的免疫保护作用。这表明DH5α(pXST1)工程菌株可以作为预防幼畜腹泻的菌苗候选株。

 
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