By using genetic recombination technique, a retroviral vector pSIR containing antisense FGF8b cDNA driven by prostate tissue specific probasin promoter was constructed as PB AS FGF8b pSIR followed with transfecting the package cell line PT 67. The prostate cancer cell line PC 3M was then infected with the replication incompetent retrovirus obtained.
Methods:The polypeptide drug included the amino sequences of BH3,K237 and DG2 domain and the peptide that could be digested by PSA. The anti-prostate cancer effects of the polypeptide prodrug on prostate cancer cell line LNCaP,22RV1(secreting PSA)and PC3,DU145(secreting no PSA)were determined by MTT test and Hoechst 33258 staining.
Method:pDR2-TK was extracted by a preparation and purification system and was evaluated by restrctional enzymatic- digestion and DNA sequencing analysis. The plasmid vector was introduced into the targeted PC-3m cells utilizing the cationic liposome-mediated gene delivery technique. In addition, mRNA and protein expression of HSV-TK were investigated by RT-PCR and SABC immunohistochemical stain respectively.