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第二内含子
相关语句
  intron 2
     1. The genetic variation in 5' -upstream and in intron 2 of porcine H-FABP gene were detected by PCR-RFLP in the above populations.
     1.应用PCR-RFLP方法对上述猪群H-FABP基因5′-上游区和第二内含子的遗传变异进行了研究。
短句来源
     Objective: To investigate the association between polymorphism of VNTR in 5-HTT intron 2 and clinical phenotype in major depression and analysis the effect of VNTR polymorphisms on depression.
     目的:分析5-HTT基因第二内含子上的VNTR多态性与抑郁症临床表型的关系,以探讨VNTR多态性对抑郁症发病的影响;
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     Genetic Variation in Intron2 of H-FABP Gene in Three Bovine Hybrids and the Relationships with Meat Quality Traits
     3个杂交牛种H-FABP基因第二内含子的遗传变异与肉品质性状的相关分析
短句来源
     Identify the expressed product by immunohistochemical method. Results The bovine α-S1 casein gene regulating sequence,consisting of 1.8 kb upstream sequence,exon 1,intron 1,the exon 2 with translation initiation site,a part of intron 2,a large part of the last intron,the last exon(containing poly A signal) which could not be translated and the whole flanking region at a length of 5.8 kb,was constructed.
     结果乳腺生物反应器特异调控序列———牛α-S1酪蛋白基因调控序列,包括其1.8kb上游序列、第一外显子、第一内含子、包含翻译起始位点的第二外显子和部分第二内含子,及其最后一个内含子的大部分、最后一个不翻译的外显子(含多聚A信号)及侧翼区全长5.8kb。
短句来源
     Pa was designed to amplify a fragment containing the third intron of chicken A-FABP gene according to the sequence of chicken A-FABP gene exon4 and intron2. A Hinf I -RFLP was discovered in chicken intron3 with two alleles designated A and B. A G-alleles were analyzed in a total of 505 samples from Beijing Oil chicken and Dwarf chicken.
     根据鸡A-FABP基因第四外显子及第二内含子序列设计特异性引物P_3,用于扩增含鸡A-FABP基因第三内含子的1.5Kb片段,在第三内含子上发现一HinfⅠ-RFLP多态位点,该位点上有两个等位基因A、B。 测序分析表明:HinfⅠ-RFLP的产生是由于700位碱基G→A的替换造成的。
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  “第二内含子”译为未确定词的双语例句
     Genetic Variation in 5'-Upstream Region and the Second Intron of H-FABP Gene in Nine Pig Breeds
     9个猪种H-FABP基因5’-上游区和第二内含子的遗传变异
短句来源
     Research on Genetic Variation in 5'-Upstream Region and the Second Intron of H-FABP Gene in Four Pig Breeds
     4个猪种H-FABP基因5’-上游区和第二内含子的遗传变异
短句来源
     Objective To evaluate the association of an intronic single nucleotide polymorphism(SNP) in cholesterol 24S-hydroxylase (CYP46) gene with Alzheimer’s disease(AD) in Chinese Han population.
     目的探讨胆固醇24S-羟化酶(cholesterol24-hydroxylase,CYP46)基因第二内含子单核苷酸多态性(single nucleotide polymorphism,SNP)与阿尔茨海默病(AD)的相互关系。
短句来源
     The genetic variation in 5′-upstream(HinfⅠ-RFLP)and the second intron(HinfⅠ*-RFLP、HaeⅢ-RFLP)of heart fatty acid-binding protein(H-FABP)gene were detected with PCR-RFLP in 286 pigs including Mashen,Shanxi white pig and their crossbred populations.
     利用PCR-RFLP技术对马身猪、山西白猪及其杂种群体共286头猪的心脏脂肪酸结合蛋白(H-FABP)基因5′-上游区(HinfⅠ-RFLP)和第二内含子内(HinfⅠ*-RFLP和HaeⅢ-RFLP)的遗传变异进行了研究。
短句来源
     There were only monomorphisms found in the FUT1, RBP4, and the second intron of H-FABP, and polymorphisms found in the ESR, FSH P , PRLR and the 5'-upstream region of H-FABP gene in Jinhua pigs.
     FUT1基因、RBP4基因以及 H-FABP 基因的第二内含子处不存在相应RFLP; ESR基因、FSHβ基因、PRLR基因和H-FABP基因的5’-上游区域在金华猪上具有多态性。
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  相似匹配句对
     Secondly, the process of drafting utilizing foreign capital
     第二
短句来源
     Second, the coursingcase of 1999~ 2000 and January?
     第二
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     Mobile Introns
     可移动的内含子
短句来源
     The intronⅠlength was 766bps and the intronⅡlength was 587bps.
     第一内含子为766bp,第二内含子为587bp。
短句来源
     Research on Genetic Variation in 5'-Upstream Region and the Second Intron of H-FABP Gene in Four Pig Breeds
     4个猪种H-FABP基因5’-上游区和第二内含子的遗传变异
短句来源
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  intron 2
Pulmonary Carcinogenesis Susceptibility-Associated Single-Nucleotide Polymorphisms in K-ras Intron 2 Affect the Binding of Facto
      
The sequence of K-ras intron 2, which has been associated with lung tumor susceptibility in inbred mouse strains, was analyzed in susceptible strain GR and in resistant strains PT and UT.
      
The results suggest that SNPs of K-ras intron 2 do not affect the level of K-ras expression but do control the binding of GATA-6, which plays an important role in lung differentiation.
      
Moreover, a third transcript with an additional exon (142 bp), which originates from intron 2, was observed in mouse cells.
      
Computer analysis predicted an alternative promoter in the 3'-terminal region of intron 2 of the rat Cp gene and showed that transcription from this promoter potentially yields an mRNA coding for a 109-kDa polypeptide.
      
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In Drosophila melanogaster,the (?) allele resullts from the insertion of the transposable elementcopia within the second intron of the while locus,The w~(?) flies have the apricot eye colour.The eye colourof (?) flies with E(?) become much lighter.One revertant of thre E(?) was generated by the irradia-tion with γ-ray,it is recessive lethal with death occuring during the larval stage.The expression of the(?)and (?) in 11 alleles of the w~+ were affacted by the E(?),but not by the E(?).These alleles are insertion...

In Drosophila melanogaster,the (?) allele resullts from the insertion of the transposable elementcopia within the second intron of the while locus,The w~(?) flies have the apricot eye colour.The eye colourof (?) flies with E(?) become much lighter.One revertant of thre E(?) was generated by the irradia-tion with γ-ray,it is recessive lethal with death occuring during the larval stage.The expression of the(?)and (?) in 11 alleles of the w~+ were affacted by the E(?),but not by the E(?).These alleles are insertion mutations of w~+ with transposable element.The regulative effect ofthe E(?) on the expression of the (?) is independent of other modifiers of the (?),such as su((?)),su(∫) and E(?).The E(?) was preliminarily located at 59F-60B on the right arm of the thrid cvhro-mosome.

果蝇中,w~n 是由于转座因子 copia 对红眼基因的第二内含子的插入造成的,表型为杏黄色眼。调控基因 E(w~n)影响着 w~n 的表达而使 w~n 果蝇眼色变浅.我们用γ射线诱变得到一个 E(w~n)的回复子,纯合致死,致死作用发生在幼虫期。在红眼基因的十一个等位基因中,E(w~n)只影响 w~n、w~(n4)、w~n、w~(np55)、w~(nRM)和 w~(nR844)的表达,它们均为红眼基因的转座因子的插入突变等位基因。但 E(w~n)~R 对这些等位基因同时失去调控作用.E(w~n)与 w~n 的其它调控基因 su(w~n),su(f)和 Doa 对 w~n 表达的调控是独立的。E(w~n)初步定位于第二染色体右臂的59F—60B的位置。

Our previous works have verified that the β-globin gene carrying larg fragments of erythroid enhancer transferred by retrovirus vector caused the unstable provirus integration and low virus titer in infected cells,but the 36bp enhancer had not this negative effect.In order to circumvent this problem,we inserted the intact β-globin gene (β)or partially IVSII deleted β-globin gene (Δβ)and truncated erythroid enhancer(36bp,292bp and 341bp)into the N2A retrovirus vector.Recombinants were transfected into Ψ-2 ecotropic...

Our previous works have verified that the β-globin gene carrying larg fragments of erythroid enhancer transferred by retrovirus vector caused the unstable provirus integration and low virus titer in infected cells,but the 36bp enhancer had not this negative effect.In order to circumvent this problem,we inserted the intact β-globin gene (β)or partially IVSII deleted β-globin gene (Δβ)and truncated erythroid enhancer(36bp,292bp and 341bp)into the N2A retrovirus vector.Recombinants were transfected into Ψ-2 ecotropic pachaging cells first, then the produced virus were used to infect PA317 amphotropic packaging cells.Virus supernatent from PA317 clonies with high virus titer and intact provirus integration was used to infect MEL cells. RNase protection assay was used to detect the expression of β-globin gene. Results showed that not only the stable provirus integration and high virus titer of the transferred genes,but also the high levels expression of β-globin gene carrying 292bp or 341bp erythroid enhancer were got.

为寻找合适长度的红系增强子,在不影响病毒滴度和前病毒整合稳定性前提下,提高外源β-珠蛋白基因的表达水平,作者将完整β基因(β)或部分缺失IVSII(第二内含子)的β-基因(Δβ)和不同长度的红系增强子(36、292、与341bp)分别克隆于反转录病毒载体N2A上,重组体分别转染ψ-2单向性包装细胞系,由ψ-2出芽的病毒颗粒感染PA317双向性包装细胞系,筛选病毒滴度较高,前病毒整合完整的PA317细胞克隆的病毒上清感染鼠红白血病(MEL)细胞,用RNase保护实验检测β-珠蛋白基因的表达。结果表明,不仅获得了稳定的前病毒整合和较高的病毒滴度,而且含292bp和341bp红系增强子的β-基因表达水平接近或达到内源性α基因。

The partial growth hormone (GH) gene and its 5′flanking regulatory region of Felis lynx was amplified by PCR with a pair of primes based on the sequences of the reported mammalian GH gene for the first time. The PCR products were cloned on Hinc Ⅱ sites of plasmid pUC19. It was determined that its length is 783 bp by the enzymes digesting and sequencing. Comparing the corresponding sequences of GH gene among Felis lynx with the other reported mammal′s, the authors concluded that there was high conservation...

The partial growth hormone (GH) gene and its 5′flanking regulatory region of Felis lynx was amplified by PCR with a pair of primes based on the sequences of the reported mammalian GH gene for the first time. The PCR products were cloned on Hinc Ⅱ sites of plasmid pUC19. It was determined that its length is 783 bp by the enzymes digesting and sequencing. Comparing the corresponding sequences of GH gene among Felis lynx with the other reported mammal′s, the authors concluded that there was high conservation in the 5′flanking sequence and exonⅠ, and exsited 89% homologus in the intronⅠ and the partial intronⅡ. Many variations of the exonⅡ occurred in the degenerate sites of the triplet code. The results of PCR shows that the GH gene of Felis lynx is a single gene in the genome.

根据已报道的哺乳动物GH基因序列设计一对引物 ,利用PCR技术直接扩增猞猁GH基因部分序列和 5′端调控区序列 ,将PCR产物克隆到质粒pUC19的HincⅡ位点 ,重组子经HindⅢ /EcoRⅠ双酶切鉴定和序列分析 ,扩增片段长度为 783bp。测序结果与其它报道的哺乳动物GH基因对应片段比较 ,5′端侧翼序列和第一外显子高度保守 ,第一内含子和部分第二内含子存在 89%同源性 ,且第二个外显子中突变多发生在密码子的兼并位点上。PCR结果和序列分析表明 ,GH基因在猞猁基因组中为单基因

 
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