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细胞杀伤效应
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  cells cytotoxicity
     The regulation of NK cells cytotoxicity by SNAP-23
     SNAP-23对NK细胞杀伤效应的调控
短句来源
     Objective To investigate the regulation of NK cells cytotoxicity by SNAP-23. Methods The expression of SNAP-23 was silenced by specific shRNA plasmid transfection using nucleofector.
     目的探讨SNAP-23对NK细胞杀伤效应的调控作用。
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  “细胞杀伤效应”译为未确定词的双语例句
     The cytotoxic effect of 211At - 3H11 McAb to target cell M85 was higher than that of 211At - IgG and Na211At .
     实验表明:~(211)At-3H_(11)McAb对靶细胞杀伤效应高于~(211)At-IgG及Na~(211)At;
短句来源
     The Study of the Killing Activity to Raji Cells of Human Peripheral Blood Mononuclear Cells Induced by IL-18 Synergistic IL-2 and IL-12
     IL-18联合IL-2和IL-12诱导外周血单个核细胞对Raji细胞杀伤效应的实验研究
短句来源
     Objective To observe the expression of CD80 transfected liver cancer cells (HepG2/ CD80) induced by IL-2、 IFN-γ, and evaluate the effection of CD80 molecule combined with IFN-γ/ IL-12 in the primary cytolytic activity against HCC.
     目的观察IFN-γ、IL-12对转染CD80基因前后肝癌细胞表面分子CD80的表达,评价CD80分子联合细胞因子对肝癌细胞杀伤效应的影响。
短句来源
     Study on killing efficacy of yeast cytosine deaminase /5 - fluorocytosine gene therapy system on K562B cell line
     酵母菌胞嘧啶脱氨酶/5-氟胞嘧啶基因治疗系统对K562B细胞杀伤效应的初步研究
短句来源
     ③RDQ had obvious inhibitory effects on enhancing secretion of TNF_αinduced by LPS stimulation(P<0.01)at the transcriptional level of ADAM17mRNA in HL_60cells.
     ③RDQ在细胞杀伤效应上、在ADAM17mRNA表达水平上均能明显抑制LPS所诱导的s-TNF-α表达和分泌的增高作用(P<0.01)。
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  相似匹配句对
     NK Cell Activating Receptors and Signalling Pathways
     自然杀伤细胞活化信号的转导和效应
短句来源
     In vitro photodynamic therapy of the human bladder cancer cells
     体外膀胱癌细胞光动力学杀伤效应的研究
短句来源
     The regulation of NK cells cytotoxicity by SNAP-23
     SNAP-23对NK细胞杀伤效应的调控
短句来源
     CTL killing activity of tumor-specific cytotoxic T lymphocyte activated by dendritic cell
     树突状细胞体外激活的CTL杀伤效应
短句来源
     They have the ability of inducing CTL reactionto clear leukemia cells.
     有较好的诱导 CTL 杀伤白血病细胞效应 ;
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  cells cytotoxicity
Protective effect of ecdysterone on PC12 cells cytotoxicity induced by Beta-amyloid25-35
      
LAK + epirubicin combined treatment increased susceptibility of MCF-7 WT and MCF-7 EPIR cells to LAK cells cytotoxicity.
      


The lethal effect of deep freezing on the tumor cells was investigated on the mouse Sarcoma 180 (S—180) and mouse Ehrlich ascite (ECA) cells.The homogenates of S-180 or ECA ascite were frozen at various temperature ranging between 0~-196℃ for 3 minutes and thawed thereafter.In another experiments, the freezing—thawing process was repeated 3 times successively.The treated tumor cells were then inoculated into normal mice to evaluate the viability of the cells.The results of the experiments indicated that both...

The lethal effect of deep freezing on the tumor cells was investigated on the mouse Sarcoma 180 (S—180) and mouse Ehrlich ascite (ECA) cells.The homogenates of S-180 or ECA ascite were frozen at various temperature ranging between 0~-196℃ for 3 minutes and thawed thereafter.In another experiments, the freezing—thawing process was repeated 3 times successively.The treated tumor cells were then inoculated into normal mice to evaluate the viability of the cells.The results of the experiments indicated that both S-180 and ECA cells did not lose their viability after a single freezing treatment in temperature range 0~-196℃ as evidenced by the growing tumors in the inoculated mice.After 3 repeated freezing,the S-180 cells lost their viability completely on freezing temperature -40~-196℃,but for ECA cells,the lethal temperature descended to -140~,-196℃.Therefore,it seems that the ability of the endurance for the freezing deterioration is different in various tumor types.The lethal effect of 3 repeated treezing treatment is stronger than that of a single one.

本文研究了不同温度的冷冻处理对小鼠肿瘤细胞的杀伤效应.小鼠 S180肉瘤匀浆细胞经过不同低温冷冻三分钟后立即升温,并接种到正常小鼠中,结果0—-196℃的各组冷冻处理均不能达到完全杀伤肿瘤细胞的效果.如连续冻融三次,则在经过-40—-196℃处理的各组细胞接种后均未在小鼠中产生肿瘤.同样,小鼠艾氏腹水癌的腹水,经过0—-196℃三分钟的冷冻处理,不能完全杀伤其肿瘤细胞;但经三次冻融,并降温到-140℃或更低,才能使其细胞完全失去活力.因此,不同种类的肿瘤细胞对冷冻的忍受能力是不同的.冻融三次要比一次对肿瘤细胞有更强的杀伤作用.

By means of 3H-TdR incorporation, inplantation of the Se-treated EAC cells, and the electronic microscopical examination, the mechanism of the cytolytic effect of SeO2 on the EAC cells were studied. The results of the experiments showed that the EAC cells incubat'ed with RPMI-1640 con-taining 1g SeO2/ml had suffered a significant cytolytic effect, which was demonstrated by the decreased carcinogenicity after the inplantation of the Se-treated cells into host mice. The incorporation of 3H-TdR was marked decreased...

By means of 3H-TdR incorporation, inplantation of the Se-treated EAC cells, and the electronic microscopical examination, the mechanism of the cytolytic effect of SeO2 on the EAC cells were studied. The results of the experiments showed that the EAC cells incubat'ed with RPMI-1640 con-taining 1g SeO2/ml had suffered a significant cytolytic effect, which was demonstrated by the decreased carcinogenicity after the inplantation of the Se-treated cells into host mice. The incorporation of 3H-TdR was marked decreased in the EAC cells after incubation with 1-40g. SeO2/ml in culture medium within 2-16 hours, the level of the 3H-TdR incorporationwas inversely proportional to the dosage and the time length of the Se treatment. The submicroscopical examinatiou showed that the shape of the nuclear chromatin and the cristae of the mitochondria were heavily injured by the treatment of SeO2. The extent of the demage in the celluiar struc-ture was proportional to the dosage of SeO2 applied to the cells. Thus the distortion of the celluiar structure were supposed to be the structural basis of the cytolytical effects on the EAC cells carried out by the SeO2 treatment. The mechanism of such a cytolytic effect were briefly discussed.

用~3H-TdR的掺入、腹腔移植瘤细胞和电镜观察等方法,对硒化物(SeO_2)在体外杀伤艾氏腹水癌(EAC)细胞的作用机理进行了探讨.实验结果表明:1μgSeO_2/ml对1×10~6/ml EAC经16小时温育后,再将它植入小鼠腹腔,其长瘤率明显下降,显示Se对EAC有杀伤作用.用含有7—40μgSeO_2/ml的培养液分别处理EAC细胞2—16小时,其~3H—TdR的掺入率,较对照组明显下降,下降的幅度与SeO_2的浓度和处理时间呈负相关,超微结构的观察表明,SeO_2杀伤瘤细胞的效应在细胞中产生了多方面的变化:主要是累及细胞核中染色质的构形及其分布,同时,线粒休内脊架渐趋消失并成为空泡.这些变化过程随着Se剂量增高和时间加长而加剧,并和~3H—TdR掺入实验的结果呈相应的关系,因此可以认为EAC的上述变化是SeO_2对EAC细胞的杀伤效应的结构基础.

After injecting the mice with staphylococcal protein A(SpA) or ApS containing staphylococci via different routs, i. e, subcutaneous or intraperitoneal, dynamic changes of ultrastructure and cytotoxic effect of maeorophages in the intraperitoneal cavity were observed by the electron microscope and the release test of ~(125)Ⅰ-udR labeled tumor target Cell DNA. It was fount that, 5-14 days after injecting the mice with SpA containing staphylococci intraperitoneally, volume of the murine peritoneal cavity macrophages...

After injecting the mice with staphylococcal protein A(SpA) or ApS containing staphylococci via different routs, i. e, subcutaneous or intraperitoneal, dynamic changes of ultrastructure and cytotoxic effect of maeorophages in the intraperitoneal cavity were observed by the electron microscope and the release test of ~(125)Ⅰ-udR labeled tumor target Cell DNA. It was fount that, 5-14 days after injecting the mice with SpA containing staphylococci intraperitoneally, volume of the murine peritoneal cavity macrophages was augmented remarkably, being 2-3 times as large as that of the control group. The surface folds of macrophages were significantly increased in comparison with control group. SpA containing staphylococci-activated macrophages were very irregular, with many pseudopodiums and finger-like process, starfishlike in shape. There was marked enhancement in cell organs-especilly, rough endoplasmic retieulars and lysosomes and there was abundant phagosomes in the cytoplasm. Furthermoren, the results showed that, 5 days after injecting SpA or SpA containing staphylococci into the mice intraperitoneally, cytotoxic effect of the mae rophages in the peritoneal cavity was increased more significantly than that of the control group(cytotoxicity 32.0±13.8%), reaching 42.0±7.8% and 46.3±11.7%. The cytotoxic effect reached peak levels(50.4±17.3% and 56.9±13.4% respectively)on 7 the day. Based on the experimental results, possible mechanism in the cytotoxic effect of macrophage increased by SpA was diseeused.

通过电镜和~(125)Ⅰ—udR标记肿瘤靶细胞DNA释放试验,对腹腔或皮下注射SpA或SpA菌体后小鼠腹腔巨噬细胞的超微结构、及其对靶细胞的细胞毒效应进行了动态观察。结果首次证明,小鼠腹腔注射SpA菌体后第5、14d,其腹腔巨噬细胞体积明显增大,可达对照组的2—3倍;皱褶明显增多,有众多的伪足和指状突起,外形极不规则,形似海星;胞质内细胞器明显增多,尤以粗面内质网和溶酶体为显著,胞质内充满吞噬体。实验进一步证明,小鼠腹腔注射SpA或SpA菌体后第5d,其腹腔巨噬细胞杀伤效应(细胞毒分别为42.0±7.8%和46.3±11.7%)比对照组(细胞毒为32.0±13.8%)明显增强,至第7d,细胞毒效应达峰值水平(分别为50.4±17.3%和56.9±13.4%)。根据实验结果,对SpA增强巨噬细胞杀伤效应的可能机理进行了讨论。

 
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