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| | The difference of growth and ammonia assimilation in rice and cucumber was analyzed under different ammonium concentration treatments by tho solution culture. The results indicated that the leaf area and chlorophyll content increased in rice but decreased sharply in cucumber with increase of ammonium concentrations. The activities of glutamine synthetase(GS) and glutamate dehydrogenase(GDH) in leaves of cucumber were lowered with 10, 20 mmol/L ammonium treatments, thus some physiological process related to ... | | 本文分析了在不同NH_4~+-N浓度(1、5、10、20 mm0I)处理一周后以水培的水稻与黄瓜叶片生长和氨同化的变化及差异。水稻能在高浓度NH_4~+N(10、20mmol)时良好生长,而黄瓜的叶面积生长量和叶绿素含量均明显减少,此时黄瓜各层叶的谷酰胺合成酶(GS)和谷氨酸脱氢酶(GDH)活力均降低,从而表现出NH_4~+-/(NH_4~+-N+酰胺-N)值的急剧升高;水稻在10和20mmolNH_4~+:N时叶片的GDH仍能保持较大的活力,H_4~+N/(H_4~+-N+酰胺-N)值均较小。结果表明,顶叶的GDH活力与NH_4~+-N/(NH_4~+N+酰胺N)可作为植物耐铵性的生理指标。 | | 文摘来源 | | Two ammonium-tolerant strains A. faecalis A1513 and A1523 had been constructed withgenetic manipulation. Field release of A1513 and A1523 had be carried out under control condition in Chi-na. The more significant increase of associative nitrogen fixation and grain yield of rice inoculated with am-monium-tolerant strains than wild type strain was observed.The long-term planned release did not effecton the soil N-fertility and ecological balance of native microorganism. It had also been proved that drug re-si... | | 以广泛分布于我国南方水稻田的固氮粪产碱菌(Alcaligenesfaecalis)为起始菌株,通过遗传重组手段构建了耐铵菌株A1513和A1523,田间累计释放面积已达万公顷以上。接种重组耐铵固氮菌株的增产幅度及田间固氮率(Ndfa%)均高于野生型。长期定点释放试验证明:在不施肥条件下,长期施用固氮菌剂不影响土壤肥力,对土著微生物群落的生态平衡也不构成威胁。重组子所携带的抗性基因在自然条件下不能扩散,并在无选择压力下逐渐丢失。根据粪产碱菌的特性、体外重组步骤以及定点释放试验结果来看,目前所构建的耐铵菌株A1513和A1523的环境风险性不大 | | 文摘来源 | | The ability of nitrogen fixation of ammonium resistant strain of Alcaligenes faecalis associated with rice plant at the soil of Nanjing had been studied by using 15 N dilution method.The results showed that the fixed nitrogen amount of glutinous rice inoculated with engineering bacteria to Alcaligenes faecalis was 39 7 kg/hm 2,and its rate of fixed nitrogen was 16 2%.The applied amount of ammonium sulphate on rice field may be ... | | 应用15N稀释法研究南京地区土壤条件下粪产碱菌耐铵型工程菌株—水稻作物联合固氮能力。结果表明,紫香糯稻用粪产碱菌耐铵型工程菌株接种后,固氮量为39.7kg/hm2,固氮率为16.2%。水稻植株利用固定的空气氮素后,可减少稻田硫铵施用量 | | 文摘来源 | |   | | << 更多相关文摘 |
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