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整合的细胞
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  integrated cell
     Oct-4 gene is specifically expressed in all the totipotent cells in mouse embtyos and undifferentiated ES cells. A constructed pG18NG was obtained by inserting the reporter geneβgeo into the oct-4 transcription elements. ES cell lines MESPU22 and MESPU13 were transfected with this construct and stable integrated cell clones were selected out.
     报道了对ES细胞群体中未分化细胞的特异标记,oct-4是一个在小鼠胚胎所有全能性细胞和未分化的ES细胞中特异表达的基因,将报告基因βgeo插入oct-4基因转录元件中构建了标记载体pG18NG,转染ES细胞MESPU22和 MESPU13后获得了稳定整合的细胞克隆。
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  “整合的细胞”译为未确定词的双语例句
     3. The monolayers of blastodermal cells were transfected with pEGFP-cENS2-Promoter and pEGFP-N respectively, the results suggest that part of blastodermal cells cultured for a week in vitro still have the CES activities. After selection by G418, we had not got the genetically modified blastodermal cells were not acquired.
     3.以pEGFP-N作为阳性对照,应用pEGFP-cENS2-Promoter载体转染胚盘单层细胞并进行G418药物筛选,表明体外培养一周胚盘细胞依然具有部分细胞的干细胞特性,但难以获得增殖性能强的稳定整合的细胞克隆。
短句来源
     Objective:To establish a stable cell line and employ the cell line as a model to study target integration of exogenous cDNA and modulation of gene expression.
     目的:建立稳定整合的细胞株,为研究外源基因定位整合机制及其对靶细胞生物特征影响,建立一个生物模型。
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     The absolute frequency of homologous recombinant event is 2×10 -7. CONCLUSION:This preliminary investigation provides a basis for future studies to generate mammary gland bioreactors through the subsequent nuclear transfer (NT) technique.
     结果 :获得了一株定点整合的细胞克隆 ,绝对基因打靶效率为 2× 10 7。 结论 :本实验结果为后续利用核移植技术制备乳腺生物反应器提供了坚实的实验基础。
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  相似匹配句对
     2. basal cell;
     基细胞;
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     Tom Clancys Splinter Cell
     细胞分裂
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     The neuroscientists studied brain functions with cell biological and molecular biological approaches.
     神经科学家们从细胞和分子生物学和整合的角度研究神经科学。
短句来源
     Results:A stable cell line,RVc23 8866 was established.
     结果:建立了稳定整合的RVc238866 细胞株。
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  integrated cell
After a 4-minute depositing process with a substrate temperature of 500°C and a source temperature of 620°C, the polycrystalline thin films can be made, so the production of high-quality integrated cell with SnO2: F/CdS/CdTe/Au structure is hopeful.
      
Integrated cell biology/biochemistry/molecular genetics laboratories: the cytoplasmic genome projects
      
These data indicate that the ECM of sponges is not an unstructured ground substance but provides the basis for integrated cell communication.
      
Several of the randomly integrated cell lines expressedlacZ at high levels in a variety of cell types present in the tumours, but most notably in epithelial cells.
      
Detection of Infectious Cryptosporidium Parvum Oocysts in Environmental Water Samples Using an Integrated Cell Culture-PCR (CC-P
      
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To study the site-specific integration in vitro,the constructed plasmid A(pKG5-hF ⅨΔ3’NeoΔ5’) and selective gene (pIC19R/MC 1-TK) were transferred into the LTK cells in culture by electroporation method.Five clones of LTK cell,into which the plasmid A had been transferred,were isolated by HAT selective medium and PCR technique.Finally,five LTK cell lines containing the defective factor Ⅸ gene were established.

用电穿孔法将质粒A(pKG5-hFⅨΔ3’NeoΔ5’)和选择基因(pIC19R/MC1-TK)同时向体外培养的LTK细胞内转移,再借助HAT培养基选择系统和PCR技术筛选出了五个细胞基因组上转入了质粒A的LTK细胞克隆,并建立了五个带有缺陷Ⅸ因子基因的LTK细胞株,即凝血因子Ⅸ定点整合细胞模型

Objective:To establish a stable cell line and employ the cell line as a model to study target integration of exogenous cDNA and modulation of gene expression.Methods:Establish recombinant retrovirus vector PLXCD;use PLXCD to transfect PA317 cells by lipofectin,screen the transfected cells by G418,culture the cells to get supernatant containing viruses;measure the titer of the virus containing supernatant and transfect RPMI 8866 cells,screen the RVc23 8866 cells by G418;analyze the expression of CD23 on the...

Objective:To establish a stable cell line and employ the cell line as a model to study target integration of exogenous cDNA and modulation of gene expression.Methods:Establish recombinant retrovirus vector PLXCD;use PLXCD to transfect PA317 cells by lipofectin,screen the transfected cells by G418,culture the cells to get supernatant containing viruses;measure the titer of the virus containing supernatant and transfect RPMI 8866 cells,screen the RVc23 8866 cells by G418;analyze the expression of CD23 on the membrane of RVc23 8866 by FACS;analyze the change of genome by In Situ Hybridization (FISH Method).Results:A stable cell line,RVc23 8866 was established.Conclusions:The results of FISH indicate that the recombinant PLXCD has integrated into the genome of RVc23 8866 cells;the results of FACS indicate that the expression of RVc23 8866 cells;the results of FACS indicate that the expression of membranal CD23 diminished and the integrated CD23 cDNA fragment has effected the expression of inherent CD23 gene.

目的:建立稳定整合的细胞株,为研究外源基因定位整合机制及其对靶细胞生物特征影响,建立一个生物模型。方法:构建重组病毒载体PLXCD;用Lipofectin 将PLXCD 转染PA317 包装细胞,用G418 筛选获得抗性细胞克隆,扩增抗性细胞收集病毒上清;测定病毒上清效价,感染RPMI8866 细胞,用G418 筛选获得抗性细胞(RVc238866 细胞) ;用FACS 分析RVc238866 细胞膜蛋白分子CD23 的表达;用细胞原位杂交(FISH) 分析细胞染色体基因组的改变。结果:建立了稳定整合的RVc238866 细胞株。结论:FISH 结果表明PLXCD 重组体已整合到细胞基因组中;FACS分析表明RVc238866 细胞膜表面CD23 分子的表达量明显降低,并说明了整合入基因组的CD23 片段影响了固有CD23 基因的表达。

Despite the wide application of ES cell technology, little is known about the pluripotent nature of ES cells. This is partly due to the heterogeneity of ES cell population in culture. This report described the specific labelling of undifferentiated cells in ES cell lines. Oct-4 gene is specifically expressed in all the totipotent cells in mouse embtyos and undifferentiated ES cells. A constructed pG18NG was obtained by inserting the reporter geneβgeo into the oct-4 transcription elements. ES cell lines...

Despite the wide application of ES cell technology, little is known about the pluripotent nature of ES cells. This is partly due to the heterogeneity of ES cell population in culture. This report described the specific labelling of undifferentiated cells in ES cell lines. Oct-4 gene is specifically expressed in all the totipotent cells in mouse embtyos and undifferentiated ES cells. A constructed pG18NG was obtained by inserting the reporter geneβgeo into the oct-4 transcription elements. ES cell lines MESPU22 and MESPU13 were transfected with this construct and stable integrated cell clones were selected out. With the experiments of in vitro cultivation, differentiation and chimeras production, it was confirmed that we have successfully labelled the undifferentiated cells in ES cell lines, and this label was both valid in vitro and in vivo.

虽然ES细胞技术的应用十分广泛,对ES细胞多能性本质的研究还不是很深入,体外培养的ES细胞群体的不均一性加大了这方面研究的难度。报道了对ES细胞群体中未分化细胞的特异标记,oct-4是一个在小鼠胚胎所有全能性细胞和未分化的ES细胞中特异表达的基因,将报告基因βgeo插入oct-4基因转录元件中构建了标记载体pG18NG,转染ES细胞MESPU22和 MESPU13后获得了稳定整合的细胞克隆。经体外培养、诱导分化、嵌合体制作等实验,证明利用该载体对ES细胞中的未分化细胞成功进行了标记,该标记在体内、体外都是有效的。

 
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