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源细胞
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  derived cell
     Results CLLEGFP can infect various human derived cell lines and express EGFP.
     结果 CLLEGFP能够感染各种人源细胞并表达 EGFP。
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     The conditioning medium of yew stem derived cell line cultures extracted after growing for 15 days can improve callus subculture survival rate by 60%.
     红豆杉茎源细胞株生长 1 5d的条件培养液也能使愈伤组织继代成活率提高 60 %.
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     The marrow stromal osteoblast can be an excellent bone derived cell and will play a key role in bone tissue engineering.
     说明骨髓基质成骨细胞可以作为良好的骨源细胞 ,并将在骨组织工程研究和应用中发挥重要作用
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     The value of skeletal muscle derived cell autologous transplantation in the treatment of stress urinary incontinence in rats
     骨骼肌肌源细胞自体移植在压力性尿失禁治疗中的价值
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     A novel efficient method of Taxus chinensis zygote embryo derived cell line initiation is reported which is suitable for large scale suspension culture to produce taxol.
     研究了建立适用于紫杉醇生物合成的红豆杉 ( Taxus chinensis)胚源细胞株的方法 .
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  “源细胞”译为未确定词的双语例句
     Methods The replication-competent HBV recombinant plasmid pHBV4.1 plus different liver-enriched transcription factor (HNF1,HNF3,HNF4,HNF6,C/EBP and RXRα/PPARα) expression plasmids were co-transfected into nonhepatic cell lines (NIH3T3,HeLa,293T,SW1353,CV-1 and COS1).
     方法用复制型HBV重组质粒PHBV4.1与肝富集转录因子HNF1、HNF3、HNF4、HNF6、C/EBP或RXRΑ/PPARΑ的表达质粒,分别共转染非肝源细胞株NIH3T3、HELA、293T、SW1353、CV-1和COS1。
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     To mimic oxidative stress in vitro, final concentration of 1.0 mmol/L hydrogen peroxide (H2O2) was added into cultured C2C12 myogenic cells.
     向传代培养的C2C12肌源细胞培养液中加入终浓度为1.0mmol/L的H2O2模拟体外氧化应激.
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     The Study on BIV Activity in Human Cells MT-4
     BIV在人源细胞MT-4中的活性研究
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     Simian parainfluenza virus 5 (SV5) is a type of RNA virus which is an ubiquitous virus in simian's, and also is a frequent contamination from simian's cell cultures.
     猴副流感病毒SV5(Simian parainfluenza virus 5)是猴群中普遍存在的一种RNA病毒,也是猴源细胞培养物中常见的污染物。
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     STAT1,STAT2,ISGF3γ,PKR, 2′5′-OAS mRNA levels from HepG 2 and HepG 2.2.15 cell lines that were treated with/without IFNα-2b at different times were detected by semi-quantitive RT-PCR.
     半定量逆转录聚合酶链反应(RT PCR)检测IFNα2b处理后不同时点的HepG2 2 15细胞和其母源细胞HepG2 细胞内信号转导和转录活化因子1(STAT1)、STAT2、干扰素刺激基因因子3γ(ISGF3γ)、2′5′寡腺苷酸合成酶(2′5′OAS)、RAN依赖蛋白激酶(PKR)的mRNA表达水平。
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  相似匹配句对
     Recent Progress in Research on Muscle-derived Stem Cells
     肌细胞研究进展
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     Immunohistochemical stains showed that the malignant cells are of the endothelial type.
     免疫组织化学提示内皮细胞性。
短句来源
     Tom Clancys Splinter Cell
     细胞分裂
短句来源
     2. basal cell;
     基细胞;
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     meleagridis from quail,and the ostrich(can′t) be infected by this C.
     鹌鹑C.
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  derived cell
The Effect of Paraquat and Hypothermia on Norflurason-Resistant Mutants of Arabidopsis thaliana and Derived Cell Cultures
      
Delayed cardioprotection in a human cardiomyocyte-derived cell line: the role of adenosine, p38MAP kinase and mitochondrial KATP
      
Differences in Rho GTPase protein expression levels based on cell sourcing (primary versus secondary-derived cell source), or as a function of surface chemistry were insignificant.
      
Culture supernatants from clones secreting the fusion protein caused inhibition of growth and protein synthesis toward K562 cells that express the human transferrin receptor but not toward a nonhuman derived cell line.
      
Arachidonic acid was able to kill all cell lines tested including two human melanoma-derived cell lines, and susceptibility to arachidonic acid was not influenced by adenovirus gene products that control susceptibility to TNF.
      
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The ten hybridomas cell lines that produce monoclonal antibodies against mycobacterium paratuberculosis have stably highly been obtained after six-time cell-fusion, through three clonings, by freezing twice in liquid nitrogen or necovery and cultivation for long time. The number of chromosomes of the hybridomas cells is more than that of cells origined hybridomas, but it is fewer than the sum of chromosomes of spleen cell and myeloma cell. The titir of supernatant of the hybridomas attained is 1/100-1/300 and...

The ten hybridomas cell lines that produce monoclonal antibodies against mycobacterium paratuberculosis have stably highly been obtained after six-time cell-fusion, through three clonings, by freezing twice in liquid nitrogen or necovery and cultivation for long time. The number of chromosomes of the hybridomas cells is more than that of cells origined hybridomas, but it is fewer than the sum of chromosomes of spleen cell and myeloma cell. The titir of supernatant of the hybridomas attained is 1/100-1/300 and that of ascitic fluids is abovel/10000. The ten monoclonal antibodies are the specific for mycobaeterium, but they have anextensive cross reaction among mycobacteria.

本试验通过6次细胞融合,经过3次反复克隆化与长时间培养和两次液氮中冻存、复苏,获得了10株稳定、高效地分泌抗副结核分枝杆菌单克隆抗体的杂交瘤细胞系。这些杂交瘤细胞的染色体数量均较其两种母源细胞有明显地增加,但少于两者之和;经培养产生的含单克隆抗体的培养上清液效价在1/100~1/300之间,腹水效价达万倍以上。这10株单克隆抗体都是分枝杆菌特异性的,但在分枝杆菌之间却有广泛的交叉反应。

The antigenic relationship between interferon prepared in porcine spleen cells (PS) and human interferon was investigated. The result showed that the PsIFN can be neutralized not only by anti-HuIFN-α sera but also by anti-HuIFN-γ, sera, the ability of neutralization of anti-HuIFN-α was higher than that of anti-HuIFN-γ sera. The antiviral activities of the porcine spleen cell interferon can be expressed on both homologous species cells and heterologous species cells. The porcine spleen cell interferon was subjected...

The antigenic relationship between interferon prepared in porcine spleen cells (PS) and human interferon was investigated. The result showed that the PsIFN can be neutralized not only by anti-HuIFN-α sera but also by anti-HuIFN-γ, sera, the ability of neutralization of anti-HuIFN-α was higher than that of anti-HuIFN-γ sera. The antiviral activities of the porcine spleen cell interferon can be expressed on both homologous species cells and heterologous species cells. The porcine spleen cell interferon was subjected to affinity chromatography on Sepharose-bound globulins from sheeps immunized with human leucocyte interferon. The porcine spleen cell interferon was efficiently absorbed and eluted from such affinity column. It was demonstrated that there is a high degree of antigenic cross reaction between porcine spleen cell interferon and human leucocyte interferon.

我们应用中和试验在不同细胞上对干扰素血清与猪脾细胞干扰素(PSIFN)及人干扰素(HuIFN-α)的中和能力进行了试验,结果证明HuIFN-α多克隆抗体具有明显的中和PSIFN的能力。而人免疫干扰素(Hu IFN-γ)抗体具有微弱的中和猪脾细胞干扰素能力。猪脾细胞干扰素不仅可在同源细胞表现出抗病毒活性,而且可在异源细胞表现出较高的抗病毒活性。应用人白细胞干扰素多克隆抗体亲和层析可有效地分离猪脾细胞干扰素,证实猪脾细胞干扰素与人白细胞干扰素之间具有高度的同源性关系。

In order to study the expression of growth hormone gene in heterologous cells,the retroviral vector pINS was used to construct the recombinant plasmid carrying the human growth hormone gene(hGH),and then it was introduced into mouse fibroblast cell line 3T3 and mouse embryonic stem cell line CCE,Levels of accumulation of hGH secreted into medium was quantified by a radioimmunoassay. Expression level of hGH gene varied considerably from one clone to another. Several 3T3 clones expressing hGH gene at high level...

In order to study the expression of growth hormone gene in heterologous cells,the retroviral vector pINS was used to construct the recombinant plasmid carrying the human growth hormone gene(hGH),and then it was introduced into mouse fibroblast cell line 3T3 and mouse embryonic stem cell line CCE,Levels of accumulation of hGH secreted into medium was quantified by a radioimmunoassay. Expression level of hGH gene varied considerably from one clone to another. Several 3T3 clones expressing hGH gene at high level were obtained,such as GHSNC 20,in which hGH concentration in medium was 3784n/ml,Integration of exogenous gene was stable. Southern blot result showed that such kind of difference was not related to the number of integrated copies of the foreign gene. The expression of ES clones transfected with pINS-GH was undetectable, and integration of the foreign gene was unstable.Southern blot result showed that hGH gene really integrated into the cellular genome, and no rearrangement of foreign gene is found at present.

为了研究生长激素基因在异源细胞中的表达,构建了携带人生长激素基因(hGH)的逆转录病毒载体pINS-GH,并将其导入小鼠成纤维细胞3T3和小鼠胚胎干细胞(ES细胞)CCE中。pINS-GH转化3T3细胞,获得了高效表达的克隆,即用放射免疫法检测到克隆培养基中有大量的人生长激素蛋白富积,如GHSNC20,富积率高达3784ng/ml,而且外源基因的整合很稳定。不同的3T3转化克隆的表达率有显著的差异,Southern杂交的结果表明,这种差异与外源基因整合的拷贝数无关。pINS-GH转化CCE细胞没有获得明显表达的克隆,而且外源基因的整合也不稳定。转化的ES细胞克隆总DNA的Southern杂交结果表明,hGH基因的确整合到细胞基因组中。目前尚未发现明显的外源DNA重排的迹象。

 
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