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独特基因
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  unique gene
     Functional Analysis of a Unique Gene (Ha122) and the Envelope Fusion Protein Gene (Ha133) in Helicoverpa Armigera Single Nucleopolyhedrovirus (HaSNPV)
     棉铃虫单核衣壳核多角体病毒(helicoverpa armigera single nucleocapsid nucleopolyhedrovirus HaSNPV)的独特基因(Ha122)和膜融合蛋白基因(Ha133)功能研究
短句来源
     Unique Gene Map—Analysis of LG Mobile Telephone's Road to World Top 4
     独特基因谱剖析LG手机进入全球四甲之路
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  “独特基因”译为未确定词的双语例句
     Although these 13 genes were highly conserved in the strains analyzed, polymorphisms were found by doing sequence alignment.
     结论:1.广州地区围产新生儿感染HCMV的临床毒株基因组中,存在着实验病毒株所缺失的UL136~UL148等13个独特基因结构,并且具有较为丰富的多态性。
短句来源
     The genomic arrangement of the ORFs in the HaSNPV Hindlll-l fragment is very different from the arrangement of their homologues in the genome of Autographa californica multiple nucleocapsid (M)NPV.HaSNPV contains 20 open reading frames so far unique among baculoviruses.
     HaSNPV基因组中含有20个独特开放阅读框架。 第三章对HaSNPV中独特基因Ha122进行了深入的分子生物学分析。
短句来源
     Chapter four reported the functional analysis of HaSNPV envelope fusion protein gene (Hal33) gene.
     已获得的研究结果表明该独特基因编码一个特异存在于ODV核衣壳中的结构蛋白。
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  相似匹配句对
     4 new genes were obtained.
     U基因
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     The gene was highly expressed in a soluble form in E.
     该基因在大肠杆菌E.
短句来源
     And these unique culture genes mainly express in ten aspects. 
     这些独特的文化基因主要表现在十个方面。
短句来源
     3. Phlegm-dampness constitution has its unique gene expression profile.
     ③痰湿体质具有独特基因表达谱特征。
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     Unique Kenya
     独特的肯尼亚(英文)
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  unique gene
Urea cycle disorders comprise a group of inborn errors of metabolism that represent unique gene-nutrient interactions whose significant morbidity arises from acute and chronic neurotoxicity associated with often massive hyperammonemia.
      
Dimeric STATs translocate to the nucleus, where they bind to specific DNA-response elements in the promoters of target genes, thereby inducing unique gene expression programs often in association with other transcription regulatory proteins.
      
The differences observed may have resulted from selection in the isolated environment (to produce a unique gene profile in terms of frequencies) or from a founder effect.
      
"Kanlow" cDNA libraries to create a gene inventory of 7,810 unique gene clusters from a total of 11,990 individual sequences.
      
This method of using short, minimally degenerate PCR primers should speed progress in the phylogenetic investigations of the TCR and related genes and lend important insights into both the origins and functions of these unique gene systems.
      
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The fragment genome of hepatitis E viruses were cloned as cDNAs, two from an epidemic outbseak of hepatitis E in the East Xinjiang of China(named HEV China XT-179 strain). cDNA sequence comparison was performed in the. 3'-terminal region, which showed 94.3% homology at nucleotide level and 99.6% homology at the amino acid level, approximally 820 base long between HEV China XT-179 and Myanmar ET1.1 strains. The peptide antigen was synthesed as their same amino acid sequence of immunoreactive epitopes, and also...

The fragment genome of hepatitis E viruses were cloned as cDNAs, two from an epidemic outbseak of hepatitis E in the East Xinjiang of China(named HEV China XT-179 strain). cDNA sequence comparison was performed in the. 3'-terminal region, which showed 94.3% homology at nucleotide level and 99.6% homology at the amino acid level, approximally 820 base long between HEV China XT-179 and Myanmar ET1.1 strains. The peptide antigen was synthesed as their same amino acid sequence of immunoreactive epitopes, and also reacted with 29 sera of patients with hepatitis and 8 sera of normal people from the East Xinjiang of China when compared with that of HEV particle antigen of China XT-179 strain. The results showed that positive reaction with the sera of patients and nagtive reaction with normal sera coincident rate was 92.6% and 100%. These results obviously demonstrates in that recombinant DNA can be used for the development of detection system and vaccine of prevention of HEV infection and identification of virological link of different geographical locations.

本文报道对中国新疆东部一起戊型肝炎(HE)流行中2例HE患者粪便中分离到的HEV中国XT-179株系基因组进行cDNA分子克隆和其免疫原性表位的血清学研究结果。cDNA序列分析表明中国XT-179株与缅甸ET1.1株之间有94.3%的核苷酸和99.6%的氨基酸具有同源性。但也有5.7%的核苷酸区别于其他HEV地理株和散发株,提示在中国流行型HEV可能存在独特的基因型,可将此毒株序列作为鉴别HEV不同毒株的病毒学依据之一。本研究还按上述HEV地理株之间共有的免疫原性表位氨基酸序列合成了多肽抗原,用于检测来自中国XT-179株系流行区的29例HEV感染者和8例正常人血清抗-HEV,与应用HEV中国XT-179株系颗粒抗原相比,两组血清抗-HEV阳性和阴性的符合率分别为92.6%和100%。证实本项研究所克隆的HEV基因组可用于制备HEV重组基因疫苗和免疫诊断制剂。

In the present study,the horizontal starch gel electrophoresis was used toanalyze 34 gene ioci from 34 Yanjin black一bone chickens.The polymorphisms werefound only in LAP,AKP一1,AKP一2,CEs一1,PEP一B The percentage ofpolymorphic loci(p)nand the average heterozy gosity(H)were 0.147 and 0.0586respectively.Gene frequencies of polymorphic loci were calculated and found higher inLAPB,aKP一1,AKP一2°,CES-1A,PEP一BA,The results showed that the geneticdiversity of Yunjin black一bone chicken is higher,the special genotypies exist...

In the present study,the horizontal starch gel electrophoresis was used toanalyze 34 gene ioci from 34 Yanjin black一bone chickens.The polymorphisms werefound only in LAP,AKP一1,AKP一2,CEs一1,PEP一B The percentage ofpolymorphic loci(p)nand the average heterozy gosity(H)were 0.147 and 0.0586respectively.Gene frequencies of polymorphic loci were calculated and found higher inLAPB,aKP一1,AKP一2°,CES-1A,PEP一BA,The results showed that the geneticdiversity of Yunjin black一bone chicken is higher,the special genotypies exist in thechicken.The Yunnan Yanjin black一bone chicken is a local breed with low level of selec-tion,have great potentialities for breeding and possess great values of protection

采用水平式淀粉凝胶电泳技术,对云南盐津乌鸡34只个体共计34个基因座位的血液蛋白及同工酶多态性进行研究,发现LAP,AKP一1,AKP一2,CKs一1,PEP-B5个座位具有多态性,多态座位百分比和平均杂合度分别为P=0.1470,H=0.0586.对多态座位基因频率进行计算发现盐津鸟鸡LAP~B,aKP-1,AKP一2°,CEs-l~A,PEP-B~A频率较高。这些结果表明云南盐津乌鸡遗传多样性程度较常见的外国鸡种高,且具有独特基因型。属选育程度低,选择潜力大的地方品种,具有较高的保种价值。

Fragment containing HSV-1 a sequence has been isolated by subcloning procedures using 3' end DIG-ddUTP labelled DNA probes. With it we developed an HSV-1 amplicon vector pHSVL which contains an HSV-1 ori,HSV-1 IE68 promotor and E. coli lacZ gene. Employing the replication functions of the helper virus HSV-1 tsK, the amplicons are wide tropic vectors, capable of entry, replication and gene expression in both Vero cells and neurons. We inserted HCMV IE promoter and firefly luciferase gene into the pHSVL and name...

Fragment containing HSV-1 a sequence has been isolated by subcloning procedures using 3' end DIG-ddUTP labelled DNA probes. With it we developed an HSV-1 amplicon vector pHSVL which contains an HSV-1 ori,HSV-1 IE68 promotor and E. coli lacZ gene. Employing the replication functions of the helper virus HSV-1 tsK, the amplicons are wide tropic vectors, capable of entry, replication and gene expression in both Vero cells and neurons. We inserted HCMV IE promoter and firefly luciferase gene into the pHSVL and name it pHLCL. The pHLCL virus,which was obtained by the same way as pHSVL,proved to be able to introduce reporter genes into immotalized cells and neurons, and express them simultaneously. Both vectors are expected to be usefull in transferring genes into postmitotic cells (including neurons),and in the study of neurobiology, neuropathology and gene therapy of the diseases of the nervous system.

利用基因工程技术分离出含有Ⅰ型单纯疱疹病毒(HSV-1)包装信号序列和HSV-1基因组复制起点序列的片段,表达外源基因所必需的启动子序列以及其下游作为报告基因的lacZ基因片段,构建成质粒型HSV-1载体pHSVL。用脂质体介导的方法可将该质粒转染入经辅助病毒HSV-1tsK株超感染的Vero细胞,并将其包装成HSV-1假型病毒颗粒,可如天然病毒一样再感染传代细胞和神经细胞并在其中进行基因表达。在pHSVL的基础上,我们还构建了一种可同时表达两种外源基因的质粒型疱疹病毒载体pHLCL,即在pHSVL中插入第二个转录单位:HCMVIE启动子和其下游的荧光素酶基因(lucgene),由此获得的pHLCL病毒接种传代细胞证明它可在其中同时表达两种报告基因.本研究成功地构建了两种质粒型HSV-1载体,其独特的基因转移方式使之在神经生理、病理及神经系统疾病的基因治疗的实验研究中都具有广阔的应用前景。

 
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