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pca细胞
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  prostatic carcinoma cell
     Methods In two human androgen-independent prostatic carcinoma cell lines DU-145 and PC-3 and one human androgen-dependent prostatic carcinoma cell line LNCaP, the expression and phosphorylation of EGFR was evaluated by using Western blot and immunoprecipitation. In LNCaP, the regulation of EGFR by androgen was measured too.
     方法 采用免疫沉淀和Westernblot方法 ,检测雄激素依赖人PCa细胞株LNCaP及雄激素非依赖人PCa细胞株DU 145、PC 3中EGFR的表达和磷酸化激活 ,测定雄激素对LNCaPEGFR表达和激活的影响。
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     Conclusions 1.In Pca tissues the VEGF-C positive expression rate increases along with the decreased tumor differentiation degree, and prompts that the VEGF-C protein expression has concern with the Pca cell growth and differentiation degree.
     结论(1)Pca组织中VEGF-C阳性表达率,随肿瘤分化程度的降低而明显增高,提示VEGF-C蛋白表达与Pca细胞生长及分化程度有关。
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     Results Apigenin could inhibit the growth-of PCa cells in a time- and dose-dependent manner (P<0.050~0.001).
     结果 Apigenin可抑制PCa细胞增殖 ,呈时间和剂量依赖关系 (P <0 .0 5 0 ) ;
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     Methods\ The secretion of IL 6 by PC 3m cells treated without or with dexamethasone were measured by using a pretty sensitive and specific enzyme immunoassay (ELISA), and the effects of exogenous IL 6, anti IL 6 monoclonal antibody and dexamethasone on the proliferation of PC 3m cells were determined by applying crystal violet assay.
     方法 采用ELISA 法测定PCa 细胞系PC3m 细胞IL6 的自分泌及Dex 处理后的分泌状况,并用结晶紫法观察IL6 、抗IL6 抗体和Dex 对PC3m 细胞生长的影响。
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     positively correlated with the differentiation stages in Pca (P<0.05). The positive rates of Cx43 in A+Bstage and in C+D stage were 42.4% (14/33) and 15.8% (3/19) (P<0.05) respectively.
     Cx43在Pca细胞中的阳性表达率与肿瘤细胞的分化程度呈正相关(P<0.05),C+D期显著低于A+B期(P<0.05)。
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     Conclusion Blockade of EGFR could prohibit the proliferation of prostate carcinoma cells,which was contributed to the decreased activation of MAPK to upregulate the p27 kip1 and arrest cell cycle.
     结论 阻断EGFR可抑制PCa细胞增殖 ,可能的机制是MAPK的活性降低 ,使 p2 7kip1的表达升高而细胞周期被阻。
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  相似匹配句对
     Tom Clancys Splinter Cell
     细胞分裂
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     The phenotype of those cells was NKH1+ CD16-.
     _-的细胞
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     PCA of NB4 cells is factor Ⅶ -dependent.
     缺FⅦ血浆组的NB4细胞未显示PCA作用。
短句来源
     STUDY ON PHYSICAL CELLULAR AUTOMATA MODEL OF ROCK MASS FAILURE(Ⅱ)——EXAMPLES
     岩体破坏演化的物理细胞自动机(PCA)(Ⅱ)——模拟例证
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  prostatic carcinoma cell
Growth inhibiting effect of estramustine on two prostatic carcinoma cell lines, LNCaP and LNCaP-r
      
The effect of estramustine (EM), estradiol-17β (E2) or 5α-dihydrotestosterone (DHT) on the growth of two human prostatic carcinoma cell lines, LNCaP and LNCaP-r was investigated.
      
The human prostatic carcinoma cell line LNCaP and its derivatives
      
Cytogenetic characterization of several androgen responsive and unresponsive sublines of the human prostatic carcinoma cell line
      
Difference in uptake of 3H-estramustine in two human prostatic carcinoma cell lines, LNCaP and LNCaP-r
      
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Immunohistochemical studies of the distribution of laminin(LN),type Ⅳcollagen(COL Ⅳ)and fibronectin(FN)in benign hyperplastic prostate tissue and prostatic carcinoma tissue were carried out.In benign prostatic hyperplasis,LN, COL Ⅳ and FN diplayed a continuous linear distribution in the basement membrane while these appeared scattered and interrupted in prcatatic carcinoma tissue and the rate of strong immunostaining being not enhanced(P>0.05),It was postulated that in prostatic carcinoma, a decrease of extracellular...

Immunohistochemical studies of the distribution of laminin(LN),type Ⅳcollagen(COL Ⅳ)and fibronectin(FN)in benign hyperplastic prostate tissue and prostatic carcinoma tissue were carried out.In benign prostatic hyperplasis,LN, COL Ⅳ and FN diplayed a continuous linear distribution in the basement membrane while these appeared scattered and interrupted in prcatatic carcinoma tissue and the rate of strong immunostaining being not enhanced(P>0.05),It was postulated that in prostatic carcinoma, a decrease of extracellular matrix in the basement membrane might play an important role in cancer metastasis.

为探讨前列腺癌(PCa)细胞的转移机制,应用免疫组织化学ABC法在良性前列腺增生(BPH)及PCa组织中对基膜连接蛋白(LN),胶原Ⅳ型蛋白(COLⅣ),纤维连接蛋白(FN)等细胞外基质(ECM)蛋白的表达进行检测,结果BPH组织中基底膜部位LN,COLⅣ及FN的染色呈连续线状分布,PCa组组织基底膜部位LN,COLⅣ及FN的阳性染色呈碎片状或断线状分布。COLⅣ在BPH与PCa组织中的强阳性率无显著性差异(P>0.05)。结果提示ECM在PCa组织中基底膜部位的明显缺失(非连续线状分布)可能是肿瘤转移的重要因素。

Microvessels were identified by immunostaining endothelial cellsfor factor VIII related antigen. We have counted microvessels within the tissue of36 cases of benign prostatic hyperplasia (BPH) and 34 cases of prostatic carcinoma(PCa). The results revealed that 1) the mean microvessel count (MVC) in PCawas significantly higher than that in BPH (P<0.01); 2) the MVC in invasive PCawas significantly higher than that in localized PCa (P<0.05); 3) the MVC of primary PCa with metastasis was higher than without tnetastasis...

Microvessels were identified by immunostaining endothelial cellsfor factor VIII related antigen. We have counted microvessels within the tissue of36 cases of benign prostatic hyperplasia (BPH) and 34 cases of prostatic carcinoma(PCa). The results revealed that 1) the mean microvessel count (MVC) in PCawas significantly higher than that in BPH (P<0.01); 2) the MVC in invasive PCawas significantly higher than that in localized PCa (P<0.05); 3) the MVC of primary PCa with metastasis was higher than without tnetastasis (P<0. 05), and 4)MVC corresponded well with the histological grading of tumor. It is postulated thattumor angiogenesis assessed by MVC corresponds well with the stage and gradingof PCa and that the infiltration and rnetastasis requires tumor angiogenesis.

为探讨前列腺癌(PCa)细胞转移的机制,应用免疫组织化学SP法在36例良性前列腺增生(BPH)及34例PCa组织中对第Ⅷ因子相关抗原进行表达,计数微血管(MV),发现PCa组织中MV数显著高于BPH组(P<0.01),浸润性PCa组MV数明显高于局限性PCa组(P<0.05),伴淋巴结转移者MV数显著高于无淋巴结转移者(P<0.05),未分化PCa组MV数显著高于高分化组及低分化组(P<0.05),提示肿瘤血管生成与PCa分期、分级密切相关,癌细胞的浸润及转移有赖于肿瘤血管的生成。

By using RT-PCR tcchnque,IL-6R mRNA expression was quantitatively assayed in 10 normal prostate samples, 15 patients with prostate carcinomas (PCa) and a PCa cell line PC-3m. The results revealed that the expression level of IL-6R gene in PCa tissues was significantly higher than that in normal prostate,and 11 of 15(73. 3%)PCa samples expressed higher levels of an IL-6R mRNA than PC-3m which was sensitive to the cytotoxic action of an IL6-toxin fusion protein. Therefore,it is suggested that IL-6 may play a role...

By using RT-PCR tcchnque,IL-6R mRNA expression was quantitatively assayed in 10 normal prostate samples, 15 patients with prostate carcinomas (PCa) and a PCa cell line PC-3m. The results revealed that the expression level of IL-6R gene in PCa tissues was significantly higher than that in normal prostate,and 11 of 15(73. 3%)PCa samples expressed higher levels of an IL-6R mRNA than PC-3m which was sensitive to the cytotoxic action of an IL6-toxin fusion protein. Therefore,it is suggested that IL-6 may play a role in the growth of PCa,and IL-6R can be a target for the delivery of therapeutic agents on PCa.

利用逆转录聚合酶链反应(RT-PCR)定量检测了10例正常人前列腺、15例前列腺癌(PCa)及 PCa细胞系PC-3m中白细胞介素6受体(IL-6R)基因表达水平。结果发现,正常人前列腺组织中IL-6R基因呈低水平表达,PCa组织中IL-6R基因表达增强,明显高于正常组(P<0.01),且73.3%(11/15)组织样本中IL-6R mRNA表达水平高于 PC-3m。而IL-6所介导的假单胞菌外毒素融合蛋白对该细胞系有强烈的杀伤作用。这表明IL-6及IL-6R与PCa的发生发展有关,并为PCa的生物学治疗的研究奠定了基础。

 
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