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备碘
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  preparing iodine
     Objective To preparing iodine labeled Tyr3]-octreotide (TOC) with conjugation labeling, and assessing the characteristic of ~ 125 I-HPNS-TOC binding to the receptors within NCI-H446 cell lines.
     目的研究联接标记法制备碘[Tyr3]-octreotide(TOC),以及125I-HPNS-TOC与小细胞肺癌株NCI-H446的受体结合特性。
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  相似匹配句对
     EXAMINATION OF GERMICIDAL EFFICACY OF SOLID IODOPHOR PREPARED BY A MODIFIED METHOD
     改良法制固体伏杀菌效果的测定
短句来源
     EXAMINATION OF GERMICIDAL EFFICACY OF NEUTRAL SOLIDIODOPHOR PREPARED BY A
     改良法制中性固体伏杀菌效果的测定
短句来源
     Identification of Iodine in Salt
     盐的检验
短句来源
     The spectrophotometric determination of microamount of atomic iodine
     (I)的分光光度法测定
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     Preparation of Phytic Acid
     植酸制
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bjective : Quality control of iodine-131 labeled

目的:对改良Mather氏法标记的大剂量 ̄(131)Ⅰ-HAb18McAb进行了临床前质量控制.方法:用常规检测方法(柱层析法、PAGE电泳法、体外培养细胞结合分析等)检测了三批样品的游离碘率、体外细胞免疫结合率、无菌、热原质及热稳定性实验等.结果:本法标记物样品热原质、无菌、毒性试验均合格, ̄[131]Ⅰ蛋白标记率≥88.3%,游离碘率≤12.6%,细胞免疫结合率>50%,白蛋白非特异标记率<6%,标记物在37℃48h内,游离碘释放率<20%,细胞免疫结合率>40%.结论:本法制备的碘标记物各项安全指标合格.热稳定实验提示超过48h的标记物游离碘及免疫活性变化较大.

Aim: To study the pharmacodynamic effect of radioimmuno-conjugate ([3]I-HAb18) on nude mice bearing human hepatoma xenograft and to determine the effective doseand median effective dose corresponding to the 30% and50% tumor suppressive rate. Methods: The nude mice weregiven [3]I-HAb18 conjugate prepared using Mather's methodvia tail-vein injections. The injection doses were 1. 85×107,4. 62 ×10s and 1. 39× 109Bq/kg, respectively. While normalsaline and epiadriamycin were the negative and positive control. Results:...

Aim: To study the pharmacodynamic effect of radioimmuno-conjugate ([3]I-HAb18) on nude mice bearing human hepatoma xenograft and to determine the effective doseand median effective dose corresponding to the 30% and50% tumor suppressive rate. Methods: The nude mice weregiven [3]I-HAb18 conjugate prepared using Mather's methodvia tail-vein injections. The injection doses were 1. 85×107,4. 62 ×10s and 1. 39× 109Bq/kg, respectively. While normalsaline and epiadriamycin were the negative and positive control. Results: Compared with the negative control, the median dose and high dose remarkably suppressed the growthof tumor, and both tumor suppressive rates were more than30%. Linear function Y=38.26 1gX-276.52 showed therelationship between logarithm and the suppressive rate ofthe three doses. The correlation coefficient was 0.9869.The effective dose and median effective dose were 1. 04×108Bq/kg and 3. 40× 108Bq/kg respectively according to thelinear function. Conclusion: The radioimmunoconjugate caneffectively inhibit the growth of hepatoma. We hope it canbe tested clinically.

观察碘[(131)Ⅰ]肝癌单抗放免治疗剂对荷人肝癌裸鼠移植瘤的疗效,并确定抑制率分别为30%和50%时的有效剂量和半数有效量(ED(50)).方法:Mather高效碘标法制备碘[(131)Ⅰ]肝癌单抗放免治疗剂,尾静脉注入荷人肝癌裸鼠体内,设低、中、高(1.85×107,4.62×108和1.39×109Bq/kg)3个剂量,并设阴性(生理盐水)、阳性(表阿霉素)对照组.结果:中剂量和高剂量的抑瘤率均大于30%,且和阴性对照组有显著性差异.三组剂量的对数值和抑制率的线性关系为:Y=38.261gX-276.52,其相关系数(r)为0.9869,由此得到有效量和半数有效量分别为1.04×104Bg/kg和3.40×108Bg/kg.结论:该制剂能有效地抑制肝癌生长,可望进入临床验证.

The procedures for isolation and purification of PC, PS, PCI and antithrombin III (ATIII) from human plasma and TM from human urine were developed. Five RIAs were also developed, on the equilibrium method, by raising the antisera in rabbits. 125I-PC, 125I-PS and !25I-ATIII were prepared using the chloramine-T method, 125I-PCI by lodogen method and I25I-TM by Bolton-Hunter method. All of their sensitivities were below 10 ug/L, and the ranges of recovery rates were 94.30% to 105.22%. The cross reactivities of...

The procedures for isolation and purification of PC, PS, PCI and antithrombin III (ATIII) from human plasma and TM from human urine were developed. Five RIAs were also developed, on the equilibrium method, by raising the antisera in rabbits. 125I-PC, 125I-PS and !25I-ATIII were prepared using the chloramine-T method, 125I-PCI by lodogen method and I25I-TM by Bolton-Hunter method. All of their sensitivities were below 10 ug/L, and the ranges of recovery rates were 94.30% to 105.22%. The cross reactivities of these methods with factor II and thrombin (Th) were negligible. The functional regulation of protein C system with flow cytometry and the five RIAs was investigated. On the basis of the five RIAs. The technique of APC-APTT was developed, which is a simple and reliable method to detect APC-resistance. A PCR was also developed for identification and verification of G1691A transition or point mutation of factor V on homozygotes and heterozygotes. Even though the diagnostic level of APC-APTT were the same as. It is observed that factor V G1691A mutation incidence on Chinese is much lower than on North European. There may be other factors about APC-resistance, such as factor VIII mutation or factor Vmutation but not on G1691A in Chinese.

从人血浆和人尿中纯化了蛋白C系统各组分及抗凝血酶Ⅲ。免疫家兔产生了抗血清,氯胺T法,Iodogen法和Bolton-Hunter法制备了碘标记化合物。采用平衡法建立了放射免疫分析方法。所有方法的最低可测限均小于10μg/L,回收率在94.30%~105.22%之间,未见与因子Ⅱ和凝血酶的交叉反应。采用所建的放射免疫分析法和流式细胞技术分析了蛋白C系统在内皮细胞表面的功能表达和调节。建立了简单、可靠,适于分析APC耐性的APC-APTT方法和可明确因子Ⅴ纯合子和杂合子G1691A点突变的聚合酶链反应方法。结果表明,中国和其他地区的黄种人因子Ⅴ G1691A点突变发生率显著低于欧洲白种人,而APC耐性并不低。提示中国等黄种人群有独立于因子Ⅴ G1691A点突变以外的因子Ⅴ或因子Ⅷ突变点存在的可能或存在其他致APC耐性因素等。

 
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