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硝基呋喃代谢物
相关语句
  nitrofuran metabolites
     Determination of Nitrofuran Metabolites in Milk Powder by High Performance Liquid Chromatography-Tandem Mass Spectrometry
     高效液相色谱/串联质谱法测定奶粉中的硝基呋喃代谢物
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  “硝基呋喃代谢物”译为未确定词的双语例句
     Poultry Tissues samples was hydrolyzed with 0.2M HCl, and derivatized at 37℃ for 16h with 2-nitrobenzaldenhyde.
     0.2mol/L盐酸水解禽肉组织中与蛋白结合的硝基呋喃代谢物,用2-硝基苯甲醛(2-NBA)37℃衍生16h。
短句来源
  相似匹配句对
     Determination of Nitrofuran Metabolites in Milk Powder by High Performance Liquid Chromatography-Tandem Mass Spectrometry
     高效液相色谱/串联质谱法测定奶粉中的硝基呋喃代谢物
短句来源
     (2) secondary metabolite synthesis;
     (2)次生代谢物的合成;
短句来源
     Advances in the Research of Resveratrol Metabolins
     白藜芦醇代谢物的研究进展
短句来源
     Poultry Tissues samples was hydrolyzed with 0.2M HCl, and derivatized at 37℃ for 16h with 2-nitrobenzaldenhyde.
     0.2mol/L盐酸水解禽肉组织中与蛋白结合的硝基呋喃代谢物,用2-硝基苯甲醛(2-NBA)37℃衍生16h。
短句来源
     SYNTHESIS AND BIOLOGICAL ACTIVITIES OF SOME NEW 3-METHYL-5-NITROFURA N DERIVATIVES
     3-甲基-5-硝基呋喃衍生物的合成
短句来源
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  nitrofuran metabolites
Triple-quadrupole LC-MS-MS for quantitative determination of nitrofuran metabolites in complex food matrixes
      
This method involves a solid-phase extraction (SPE) of nitrofuran metabolites and nitrofuran parent drugs, a derivatization by 2-nitrobenzaldehyde for 17?h, and finally a clean-up by SPE.
      


A method for determination of the four metabolites of nitrofuran antibiotics such as 3-amino-2-oxazolidinone (AOZ), 3-amino-5-morpholinomethyl-2- oxazolidinone (AMOZ), semicarbazide (SEM) and 1-aminohydantoin(AHD) in Poultry Tis- sues by LC-MS-MS was developed. Poultry Tissues samples was hydrolyzed with 0.2M HCl, and derivatized at 37℃ for 16h with 2-nitrobenzaldenhyde. The derivatized solutions were adjusted to pH=7.4, and then were cleaned up with LiChrolut EN cartridge, the target compounds were eluted with...

A method for determination of the four metabolites of nitrofuran antibiotics such as 3-amino-2-oxazolidinone (AOZ), 3-amino-5-morpholinomethyl-2- oxazolidinone (AMOZ), semicarbazide (SEM) and 1-aminohydantoin(AHD) in Poultry Tis- sues by LC-MS-MS was developed. Poultry Tissues samples was hydrolyzed with 0.2M HCl, and derivatized at 37℃ for 16h with 2-nitrobenzaldenhyde. The derivatized solutions were adjusted to pH=7.4, and then were cleaned up with LiChrolut EN cartridge, the target compounds were eluted with ethyl acetate and diluted with mobile phase. MS detection was performed in the positive ion mode using multiple reaction monitoring (MRM). The standard calibration curve for four kinds of metabolites were obtained through the synchronized operation of using blank Poultry Tissues sample fortification standard and Poultry Tissues samples for a quantitative analysis per the external standard. In the range of fortification concentration of 0.5~5μg/kg, the average recoveries for these four kinds of metabolites fell within 70.2%~89.1%. Limit of detection for AOZ and AMOZ was 0.1μg/kg, limit of detection for SEM and AHD 0.2μg/kg. The relative standard deviation (CV) was between 5.59%~10.10% (n=10).

本文建立了用高效液相色谱-串联质谱(LC-MS-MS)测定禽肉组织中3-氨基-2-唑烷基酮(AOZ)、5-甲基吗啉-3-氨基-2-唑烷基酮(AMOZ)、氨基脲(SEM)和1-氨基-2-内酰脲(AHD)4种硝基呋喃类抗生素代谢物的方法。0.2mol/L盐酸水解禽肉组织中与蛋白结合的硝基呋喃代谢物,用2-硝基苯甲醛(2-NBA)37℃衍生16h。上清液调pH=7.4后,用LiChrolutEN固相萃取柱净化,乙酸乙酯洗脱,流动相定容。采用电喷雾电离,正离子扫描,多反应监测(MRM)模式检测,标准曲线用空白禽肉样品添加标准与样品同步操作获得并用其进行外标定量。在添加浓度0.5~10μg/kg范围内,四种代谢物的平均回收率在70.2%~89.1%,检出限AMOZ、AOZ为0.1μg/kg,SEM、AHD为0.2μg/kg。10次测定结果相对标准偏差在5.59%~10.10%之间。

 
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