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   已知的基因 的翻译结果: 查询用时:0.506秒
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已知的基因
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  known gene
     After cloning,sequencing and searching with Blast and NewCpGseek programs,the result showed that all of them were typical CpG island sequences,four fragments had 99%~100% homology to regions on human chromosome 2,7,9 and 10,respectively,but only one revealed to be known gene.
     通过克隆、测序和Blast、NewCpGseek软件分析 ,发现所有的片段均为典型的CpG岛 ,有 4个片段与人 2、7、9、10号染色体上的同源性为 99%~ 10 0 % ,但只有 1个是已知的基因
短句来源
     Gene chip contained 9 984 human cDNA segment, which was prepared and provided by City University of Hongkong (UniGEMV2 cloneset known gene and ESTs were purchased from Incyte Company), Superscript Ⅱ reverse transcriptase was provided by Gibco-BRL Company. Fluorochrome Cy3 & Cy5 was the products of Amersham Pharmacia Company.
     基因芯片含9,984个人类cDNA片段,由香港城市大学准备和提供(UniGEMV2cloneset已知的基因和ESTs购自Incyte公司),SuperscriptⅡ反转录酶由Gibco-BRL公司提供,荧光染料Cy3&Cy5为AmershamPharmacia公司产品。
短句来源
     In the 5 sub clones, three clones were homologous to the known gene.
     对其中 5个差异条带进行了DNA序列测定 ,有 3个与已知的基因高度同源 ,分别为tPA、Kiaa0 372和LDLC。
短句来源
     Conclusions N9 is probably a known gene segment, which may play a role in the pathogenesis and progress of MG with different types of thymus abnormalities. Further studies are needed to confirm this mechanism.
     结论 N9可能是已知的基因片段 ,它可能在伴不同类型胸腺异常MG的发病或发展中起某种作用 ,其具体作用还有待于进一步研究
短句来源
     Because some gene features have not been understood and a great deal of small differences among different species, a kind of new self\|learning program was established, which could update those original data after storing some known gene sequences,so as to adapt to and recognize the gene structures of those different species.
     考虑到基因的许多特征还不为人们所了解 ,而且不同物种之间基因结构又有一定的差异 ,所以还开发了程序自学习功能 ,不断地存储已知的基因 ,再据此改变一些已有固有数据 ,以便更好地适应和了解不同生物基因结构的特异性 ,更加准确地寻找未知基因的位置。
短句来源
  “已知的基因”译为未确定词的双语例句
     When comparing with other known viruses including Chinese isolates,the two stains shared closer identity with the isolates from Indonesia,and the rates of homogeny of N and G gene were 92.1%-93.2% and 91.9%-92.1% at the nucleotide level,97.5%-98.6% and 96.0 %-96.2% at the amino acid level,respectively.
     与已知的基因1型狂犬病毒相比,2株病毒与印度尼西亚从犬中分离到的2株病毒同源性最高,N基因与G基因核苷酸同源性分别为92.1%~93.2%和91.9%~92.1%,氨基酸同源性分别为97.5%~98.6%和96.0%~96.2%。
短句来源
     PCR-RFLP was used for detecting the cry-type genes from new Bacillus thuringiensis strains, T4-3 and cyz-13. The results showed that there was five cry-type genes including cry1Aa, cry1Ac, cry1Ag, cry2Ac and cry1Ia in Bt T4-3 strains;
     新菌株cyz-13,T4-3的基因型进行分析。 结果表明,cyz-13含有cry1Aa,cry1Ac,cry1Bc,cry2Ab4种基因型,其中cyz-13菌株的PCR产物的酶切片断类型不同于已知的基因类型;
短句来源
     The BLAST search of 23 genes revealed that 12 were homologous to functionally known genes, 4 were homologous to functionally unknown entries, and 7 were novel without any relatives.
     所代表的 2 3个基因搜索结果显示 ,12个与功能已知的基因同源 ,4个与功能未知的基因同源 ,7个为新发现的基因 ;
短句来源
     The sequence of WT9 has no significant similarity with the genes submitted.
     WT9与已知的基因无同源性。
短句来源
     Results ①Forty-four gene fragments differentially expressed were obtained. Ten gene fragments sequence were detected and 7 of them had higher homology with known genes.
     结果①得到44条差异片段,测序10条片段,有7条与已知的基因有较高同源性。
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  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Finally,216 different genes were identified preliminarily.
     其中24个ESTs属于已知的小麦基因;
短句来源
     The sequence of WT9 has no significant similarity with the genes submitted.
     WT9与已知的基因无同源性。
短句来源
     The gene was highly expressed in a soluble form in E.
     该基因在大肠杆菌E.
短句来源
     Intelligence: the known and the unknown
     智力:已知的和未知的
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  known gene
The LMW-Eb is a novel gene and the LMW-Em has an identical sequence with a known gene.
      
There is no homologous known gene found after searching by blasting this sequence to non-redundancy nucleotide database.
      
All currently known gene defects causing PD alter the ubiquitin-proteasomal pathway of protein degradation.
      
Methods: PCR amplification was performed by using primers based on the known gene sequence of hTERT.
      
We selected a 660-bp sequence of the MMTVenv gene with low homology to HER (or any other known gene) and searched for a sequence homologous to it, using the polymerase chain reaction (PCR).
      
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AIM: To clone and analyze the functional characteristics of genes expressed differenty in cultured primary human PDLF in comparison with GF. METHODS: Subtractive cDNA library of PDLF was constructed with a modified gene cloning technique which is based on PCR and subtractive hybridization. Genes known to GeneBank were analyzed concerning their functional characteristics. RESULTS: 14 genes were cloned and the 10 known genes are responsible for intracellular process of cell differentiation and matrix synthesis...

AIM: To clone and analyze the functional characteristics of genes expressed differenty in cultured primary human PDLF in comparison with GF. METHODS: Subtractive cDNA library of PDLF was constructed with a modified gene cloning technique which is based on PCR and subtractive hybridization. Genes known to GeneBank were analyzed concerning their functional characteristics. RESULTS: 14 genes were cloned and the 10 known genes are responsible for intracellular process of cell differentiation and matrix synthesis and secretion. CONCLUSION: This study suggests the possible potential of PDLF to differentiate and comparatively more active intracellular protein synthesis and secretion. [

目的 :克隆人牙周膜成纤维细胞 (periodontalligamentfibroblast,PDLF)与牙龈成纤维细胞 (gin givalfibroblast,GF)差异表达基因并初步分析其中已知基因的功能特征。方法 :采用基于PCR和消减杂交的基因克隆技术构建人PDLF与GF差异表达基因的扣除文库 ,克隆人PDLF与GF差异表达基因 ,对已知基因的功能特征进行分析。结果 :成功克隆到 14个人PDLF与GF细胞差异表达基因 ,其中 10个为已知基因。已知基因的功能多与细胞分化和细胞外基质的合成、分泌有关。结论 :牙周膜成纤维细胞相对于牙龈成纤维细胞可能具有一定的分化潜能和相对旺盛的蛋白合成与分泌活性

Aim To investigate cholesterol-induced atherogenesis molecular mechanism, clone and analyse the differentially expressed genes in vascular endothelial cell induced by cholesterol. Methods Suppression subtractive hybridization (SSH) method is used to isolate the differentially expressed cDNA in human umbilical vein endothelial cell induced by cholesterol. After sequencing and homology analysis, ten differentially expressed cDNA fragments are selected and analysed by reverse transcription-polymerase chain...

Aim To investigate cholesterol-induced atherogenesis molecular mechanism, clone and analyse the differentially expressed genes in vascular endothelial cell induced by cholesterol. Methods Suppression subtractive hybridization (SSH) method is used to isolate the differentially expressed cDNA in human umbilical vein endothelial cell induced by cholesterol. After sequencing and homology analysis, ten differentially expressed cDNA fragments are selected and analysed by reverse transcription-polymerase chain reaction(RT-PCR). Results Twenty-three differentially expressed cDNA fragments have been isolated. Seventeen of them are known genes, the other six are unknown genes. The known genes include thrombospondin-1, proteasome subunit (type and so on). The ten elective cDNA fragments (including four known genes and six unknown genes) that are identified by RT-PCR have the same expressed tendency as SSH discovery. Six differentially expressed unknown cDNA fragments have been accepted by GenBank as novel expressed sequence-tags (EST). Conclusion The high level expression of thrombospondin-1 and the low level expression of proteasome subunit (type) in endothelial cell induced by cholesterol may be related to cholesterol-induced atherogenesis.

为探讨高胆固醇致动脉粥样硬化的分子机理 ,利用抑制消减杂交技术克隆胆固醇诱导脐静脉内皮细胞产生的差异表达基因 ,并对其表达进行初步研究。经顺、反两向消减杂交和巢式聚合酶链反应扩增 ,获得了差异表达的cDNA片段 ,克隆化后挑选部分进行测序、同源性比较及应用半定量逆转录—聚合酶链反应分析部分差异基因的表达情况 ,得到 2 3个差异表达的cDNA片段 ,其中 6个新差异表达cDNA片段被GenBank接受。已知基因中的血小板反应蛋白 1和蛋白酶体亚单位基因表达的改变可能与高胆固醇致动脉粥样硬化作用相关

Objective To evaluate the role of differentially expressed genes in human prostate carcinoma initiation and progression. Methods cDNA microarray chips which consist of a set of 4366 human genes were used to investigate the gene expression pattern of samples from human prostate cancer and normal prostate tissues. Results Of 4366 genes,287 genes differentially expressed in prostate carcinoma and normal prostate tissues were screened out,including 165 unknown genes and 122 known genes. Among the 122 known...

Objective To evaluate the role of differentially expressed genes in human prostate carcinoma initiation and progression. Methods cDNA microarray chips which consist of a set of 4366 human genes were used to investigate the gene expression pattern of samples from human prostate cancer and normal prostate tissues. Results Of 4366 genes,287 genes differentially expressed in prostate carcinoma and normal prostate tissues were screened out,including 165 unknown genes and 122 known genes. Among the 122 known genes we further identified 20 up-regulated and 36 down-regulated genes. Conclusions Our results suggest that significantly differential expression of genes may be associated with the pathogenesis and progression of the prostate cancer.

目的 探讨前列腺癌发生发展中相关基因群的表达意义。 方法 应用含有 4 36 6条人类全长基因cDNA基因芯片研究一组前列腺癌及正常前列腺组织基因表达谱的差异性。同时进行生物信息学分析。 结果 在前列腺癌与正常前列腺组织间存在差异表达的基因 2 87条 ,未知基因16 5条 ,已知基因 12 2条。已知基因中有显著差异表达基因 5 6条 ,其中上调基因 2 0条 ,下调基因 36条。 结论 生物信息分析显示 :显著差异表达基因与肿瘤的发病机理可能存在相关性。

 
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