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| | Transgenic crop breeding is a modern molecular breeding technique based on biotechnologies such as in vitro DNA recombination,genetic transformation and so on.It can break the natural barriers of gene exchange among organisms in different kindom,phyrum,class,order,family and genus,greatly enlarges the gene pool available for crop breeding,and opens a brand way for crop improvement.Development and wide application of transgenic crops will definitely push agriculture advancement.Transgenic crop breeding wi... | | 作物转基因育种是基于DNA体外重组、遗传转化等生物技术的一项现代分子育种技术。它可打破遗传物质分类上门、纲、目、科属的界限,实现基因的界间转移,从而大大拓宽作物遗传改良可资利用的基因源,为农作物品种改良开辟了崭新的途径。转基因作物的育成和大面积应用,将极大地推进农业生产的发展,因此,作物转基因育种将是农业新技术革命中的核心技术。在美国、加拿大等国,抗虫、耐除草剂转基因玉米、棉花、大豆、油菜等的育成和推广,已对农业生产产生了一定的影响。为此,作者分析了我国作物转基因育种的现状和存在的问题,提出了发展我国转基因育种工作的若干建议。 | | 文摘来源 | | The worldwide success in crop biotechnology has been made in recent years, and a considerable mount of genetically engineered plants have been produced, with the characters of insect resistance, disease resistance, herbicide tolerance and high quality & quantity. Meanwhile, the industrialization of them has been speeded up with the help of governments. It is expected that the agro-biotechnology will become one of the major industrial fields of many countries. We can also see its great prospect in resolving ... | | 近些年,农作物生物技术在世界范围内取得了飞速的发展,一批抗虫、抗病、耐除草剂和高产优质的农作物新品种已培育成功。与此同时,其产业化步伐在各国政府的大力参予下正在加快,预计在下个世纪初期将成为许多国家经济的重要支柱产业之一,并在解决人类目前所面临的粮食安全、环境恶化、资源匮乏、效益衰减等问题上发挥巨大作用。本文综述了农作物生物技术的发展现状,对下一世纪该学科的发展动态作了展望,并就我国农作物生物技术的发展提出了具体建议 | | 文摘来源 | | Based on the heterogenous genes usually used in transgenic crops,the PCR technique was performed with primers derived from CaMV 35S promoter(35S-promoter,originated from cauliflower mosaic virus), NOS terminator(nopaline synthase-terminator,derived from Agrobacterium tumefaciens),EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene,and CryIA(b) (delta-endotoxin,evolved from Bacillus thuringiensis subsp.kurstaki)gene to detect transgenic agents from feed raw materials of soybean dregs an... | | 采用PCR检测方法从饲料的主要原料豆粕、玉米蛋白粉中成功地检出启动子 35S (35S -promoter ,origi natedfromcauliflowermosaicvirus)、终止子NOS (nopalinesynthase -terminator,derivedfromAgrobacteriumtumefa ciens)、耐除草剂基因EPSPS(5 -enolpyruvylshikimate - 3-phosphatesynthase)和抗虫基因CryIA(b) (delta -endo toxin ,evolvedfromBacillusthuringiensissubsp .kurstaki)等转基因成分 ,并通过扩增玉米自身蛋白基因Zein(apro teinextractedfromcorngluten)及大豆自身基因Lectin(chitin -bindingprotein)的引物和阴阳性对照、阴阳性质控 ,避免假阳性、假阴性结果。该方法已在口岸进口饲料原料转基因检测中得到初步应用。 | | 文摘来源 | |   | | << 更多相关文摘 |
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