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gprotein
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  g-protein
     THE REGULATORY EFFECT OF NUCLEOSIDE DIPHOSPHATE KINASE ON G-PROTEIN AND G-PROTEIN MEDIATED PHOSPHOLIPASE C
     THE REGULATORY EFFECT OF NUCLEOSIDE DIPHOSPHATE KINASE ON G-PROTEIN AND G-PROTEIN MEDIATED PHOSPHOLIPASE C
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  相似匹配句对
     protein.
     protein
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     TPR-repeat protein ;
     TPR-repeat protein ;
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     min 1 .mg 1 protein,respectively.
     min-1.mg-1protein
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     The mAbs were purified by protein G immunoaffinity method.
     Protein G免疫亲合层析法纯化单抗;
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  gprotein
Recently, the small Gprotein Rho has attracted much attention.
      
Gprotein-coupled receptors (GPCRs) represent one of the most important categories of membrane proteins that play important roles in signaling pathways.
      
Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0?mg?gprotein-1?h-1, respectively.
      
Biomass yieldin relation to light energy was determined as gprotein per mol of photons (400-700 nm).
      
Antecedent ethanolincreased FAT 18 hr after SO from 0.105 0.048 μg/gprotein to 0.258 ± 0.054 and AP lasting 18 hrfrom 0.331 ± 0.072 to 0.695 ± 0.110.
      
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A receptor radioassay system for thedetermination of the number of bindingsites of HCG receptors is presented. The homogenates were prepared fromrat ovaries. The concentration of PEG foroptimal precipitation of receptor bound HCGwas 7% (W/V)and the binding of ~(125)I-HCGto the ovarian homogenates at 30℃ showedtime-dependent. Significant binding occur-red within 30 min of incubation, with aplateau for 1-3 hours. Nonspecific, bindingshowed no appreciable change during the 4hours incubation. The concentration of...

A receptor radioassay system for thedetermination of the number of bindingsites of HCG receptors is presented. The homogenates were prepared fromrat ovaries. The concentration of PEG foroptimal precipitation of receptor bound HCGwas 7% (W/V)and the binding of ~(125)I-HCGto the ovarian homogenates at 30℃ showedtime-dependent. Significant binding occur-red within 30 min of incubation, with aplateau for 1-3 hours. Nonspecific, bindingshowed no appreciable change during the 4hours incubation. The concentration of ~(125)I-HCG influenced the binding of ~(125)I--HCG.The maxium binding occurred when ovarianhomogenates were incubated with 10 ng of~(125)I-HCG. The binding of ~(125)I-HCG couldbe progressively displaced by the additionof increasing amounts of unlabeled HCG.The binding in the absence of unlabeledhormone (B_0) was 32%. By a Scatchard plotthe data yielded a value of 0.51×10~(-2)M/μgprotein for binding sites. The retention by ~(125)I-HCG of bindingaffinity for receptor sites in the rat ovarianhomogenate indicates that the labeled gona-dotropins retain biologic activity. The totalspecific binding of ~(125)I-HCG amounted to25% of the total radioactivity when thepreparation of ovarian homogenates was usedin an amount of 5μg protein. This studyhas provided a basis for the further radioli-gand receptor assay in HCG receptor research.

作者用自制的~(125)I-HCG及大鼠卵巢细胞膜受体制剂建立了绒毛膜促性腺激素受体的放射分析法。研究了一些实验条件对分析的影响。结果显示,5μg蛋白受体制剂。3~10ng~(125)I-HCG及未标记HCG(0~1000IU)在30℃振荡保温2~3小时可获得良好的结合抑制曲线。分离剂选用7%聚乙二醇较好。

Thirtycompensatedposthepatiticcirrhoticpatientswereequallydividedinto3groups,eachgroupcomprised10patients.Group1weregivenroutinehospitaldietandsupportivetreatment.Group2weretreatedbythesamesupportivetreatmenttogetherwithahighcaloricvegetraiandietwhichprovidedadailyintakeof2263kcaland95gprotein.Group3weretreatedwiththesamesupportivetreatmentandsamehighvegetarianproteindietaspatientsingroup2,butwithadditionaltabletsofacyclovirgiveninthedosageof0.2gfourtimesperdaytogetherwithsometraditionalChinesemedicine.After2monthsofdietarytreatment,allthepatientsingroup2and3showedamarkedincreaseinserumalbuminandtransferrin,anincreaseof24hurinarycreatinineoutputandcreatinineheightindexwithadecreaseof24hurinaryoutputofureanitrogen.TherevertedA/Gratiowascorrectedandthenegativenitrogenbalancebecamepositive.Theproteinsyntheticrateturnedtobecomegreaterthanthecatabolicrateandthebodyproteinfluxdecreasedasmeasuredbythe15N-glycinetracerkineticstudy.Theliverfunctiontestsmeasuredbyplasmamethacetinclearancetest,leucinedecarboxylaseactivityandprealbuminfractionalsynthesisrateimprovedinallthesepatients....

Thirtycompensatedposthepatiticcirrhoticpatientswereequallydividedinto3groups,eachgroupcomprised10patients.Group1weregivenroutinehospitaldietandsupportivetreatment.Group2weretreatedbythesamesupportivetreatmenttogetherwithahighcaloricvegetraiandietwhichprovidedadailyintakeof2263kcaland95gprotein.Group3weretreatedwiththesamesupportivetreatmentandsamehighvegetarianproteindietaspatientsingroup2,butwithadditionaltabletsofacyclovirgiveninthedosageof0.2gfourtimesperdaytogetherwithsometraditionalChinesemedicine.After2monthsofdietarytreatment,allthepatientsingroup2and3showedamarkedincreaseinserumalbuminandtransferrin,anincreaseof24hurinarycreatinineoutputandcreatinineheightindexwithadecreaseof24hurinaryoutputofureanitrogen.TherevertedA/Gratiowascorrectedandthenegativenitrogenbalancebecamepositive.Theproteinsyntheticrateturnedtobecomegreaterthanthecatabolicrateandthebodyproteinfluxdecreasedasmeasuredbythe15N-glycinetracerkineticstudy.Theliverfunctiontestsmeasuredbyplasmamethacetinclearancetest,leucinedecarboxylaseactivityandprealbuminfractionalsynthesisrateimprovedinallthesepatients.

30例代偿期肝炎后肝硬化患者分为3组,组1,10例,服用医院常规食谱,并进行支持治疗;组2,10例,服用高能量植物蛋白食谱和支持治疗;组3,10例,服用高能量植物蛋白食谱,支持治疗,加用阿昔洛韦和中药。二个月后,组2、组3患者的整体蛋白质代谢紊乱纠正,表现在蛋白质流量减少,合成率增加,分解率下降,合成率大于分解率。

Objective\ The age related alterations in vascular function are correlatedto the changes of G proteins.\ \ Methods\ The levels of Gprotein α subunits mRNA were quantified in the aorta and cultured aortic smooth muscle cells(SMC)of adult(6 month old)and senescent(24 month old)Fischer rats.Northern blot and dot blot analysis were used to determine the changes of Gsα,Gi 2α,Gi 3α and Goα mRNA.\ \ Results\ Northern blot reveales two bands of Goα mRNA(3 5 and 3 8kb)in aorta and aortic SMC,one band of Gi...

Objective\ The age related alterations in vascular function are correlatedto the changes of G proteins.\ \ Methods\ The levels of Gprotein α subunits mRNA were quantified in the aorta and cultured aortic smooth muscle cells(SMC)of adult(6 month old)and senescent(24 month old)Fischer rats.Northern blot and dot blot analysis were used to determine the changes of Gsα,Gi 2α,Gi 3α and Goα mRNA.\ \ Results\ Northern blot reveales two bands of Goα mRNA(3 5 and 3 8kb)in aorta and aortic SMC,one band of Gi 3αmRNA(3 2kb),one band of Gsα mRNA(1 8 kb),but there were two bands (2 1 and 1 7kb)in aorta while only one band(2 1kb)was detected in aortic SMC for Gi 2 αmRNA.There were no significant changes in the abundance of any of these Gα mRNAs in the aorta during aging.In aortic SMC,the levels of Gi 2 α mRNA increased significantly during aging( P <0 05)while Goα,Gsα in creased slightly.\ \ Conclusions\ The transcriptions of Gα subunits in the aortic SMC changed significantly during aging,the trends of their changes were consistent with that in the aorta and the extents were bigger.

目的衰老引起的血管功能减退与G蛋白有关,本实验研究24月龄大鼠主动脉及平滑肌细胞(SMC)中G蛋白α亚基的信使核糖核酸(mRNA)变化。方法用Gsα、Gi2α、Gi3α和Goα的cDNA探针按Northernblot和dotblot法对6和24月龄大鼠主动脉和主动脉SMC相应的G蛋白α亚基mR-NA进行定量分析。结果Goα在主动脉及其SMC中均为2条带(3.5和3.8kb),Gi3α和Gsα在二者中各为1条带(3.2和1.8kb),但Gi2α主动脉有2.1kb的强带和1.7kb的弱带,而SMC只有2.1kb的1条带。主动脉中各Gα均无显著变化,而SMC中Goα、GsαmRNA轻度升高,Gi2α显著升高(P<0.05)。结论主动脉SMC中G蛋白α亚基的mRNA表达是有变化的,其趋势与主动脉基本一致,但变化更明显一些。

 
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