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   溶解性周期 的翻译结果: 查询用时:0.444秒
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溶解性周期
相关语句
  lytic cycle
     Induction of Lytic Cycle Replication of Kaposi' s Sarcoma-associated Herpesvirus (KSHV)by Human Herpesvirus 6
     HHV6感染激活卡波济肉瘤相关疱疹病毒(KSHV)的溶解性周期复制
短句来源
     These results suggested that HHV6 infection could activate KSHV lytic cycle replication.
     提示:HHV6感染可激活KSHV的溶解性周期复制。
短句来源
     Conclusion HIV-1-related inflammatory cytokines are responsible for the induction of KSHV lytic cycle replication in HUVEC and the induction may be, at least in part, mediated by Rta promoter of KSHV.
     结论HIV-1感染相关的炎性细胞因子是诱导HUVEC中KSHV溶解性周期复制的因素,而且该过程至少有部分由KSHV的Rta启动子介导。
短句来源
     Results Recombinant cytokines, such as gamma interferon(IFN-γ), hepatocyte growth factor/scatter factor (HGF/SF), and oncostatin M(OSM) not only induce KSHV lytic cycle replication, but also stimulate Rta promoter activity in latently infected HUVECs.
     结果包括干扰素-γ(IFN2γ)、肝细胞生长因子P扩散因子(HGFPSF)及制瘤蛋白M(oncostatinM,OSM)在内的重组细胞因子不仅可诱导HUVEC中潜伏感染的KSHV发生溶解性周期复制,而且能增强Rta启动子活性。
短句来源
  “溶解性周期”译为未确定词的双语例句
     Reactivation of latent Kaposi′s sarcoma-associated herpesvirus in human umbilical vein endothelial cells by HIV-1-related inflammatory cytokines
     HIV-1相关炎性细胞因子诱导人血管内皮细胞中潜伏感染KSHV的溶解性周期复制
短句来源
     BCBL-1 cells derived from primary effusion lymphoma (PEL) were fused with human herpesvirus 6 (HHV6)-infected JJhan cells to create heterokaryons and subsequently harvested at the different time points after fusion.
     运用细胞融合、细胞混合培养、条件培养基培养和病毒直接刺激等方法,研究人类疱疹病毒6型(HHV6)对卡波济肉瘤相关疱疹病毒(KSHV)溶解性周期复制的影响。
短句来源
  相似匹配句对
     A BRIEF DISCUSSION OF CYCLE
     周期浅析
短句来源
     Then b is called the base of A. and p the period of A.
     p为A的周期.
短句来源
     These results suggested that HHV6 infection could activate KSHV lytic cycle replication.
     提示:HHV6感染可激活KSHV的溶解性周期复制。
短句来源
     PHACOLYTIC GLAUCOMA (ABSTRACT)
     晶体溶解性青光眼
短句来源
     Phacolytic Glaucoma:Twelve Cases
     晶体溶解性青光眼
短句来源
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  lytic cycle
The labeled mRNAs were then used as probes in Southern hybridization reactions to identify restriction fragments in the phage genome where new transcripts were initiated during progression of the phage lytic cycle.
      
The most commonly studied gene, beige (Bg), has been found to block a postrecognition event in the lytic cycle.
      
Furthermore, the induction of the HHV-8 lytic cycle was not observed under the staurosporine treatment.
      
The S-adenosylhomocysteine hydrolase (SAH) and 14-3-3 ζ/phospholipase A2 (PLA2) are transcriptionally activated in parallel to the induction of the Epstein-Barr virus (EBV) lytic cycle by the ganglioside IV3NeuAc-nLcOse4Cer.
      
Expression of EA-D, BZLF1, and BHRF1 was increased in response to both, SAH- and 14-3-3 ζ/PLA2 overexpression indicating the initiation of the EBV lytic cycle.
      
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BCBL-1 cells derived from primary effusion lymphoma (PEL) were fused with human herpesvirus 6 (HHV6)-infected JJhan cells to create heterokaryons and subsequently harvested at the different time points after fusion. Meanwhile, BCBL-1 cells were cultured with HHV6-infected JJhan cells by direct cell-cell contact and collected at the different time points. Furthermore, BCBL-1 cells were cultured in the conditioned medium that were the normal and heat-inactivatd supernatants of HHV6-infected JJhan cells and also...

BCBL-1 cells derived from primary effusion lymphoma (PEL) were fused with human herpesvirus 6 (HHV6)-infected JJhan cells to create heterokaryons and subsequently harvested at the different time points after fusion. Meanwhile, BCBL-1 cells were cultured with HHV6-infected JJhan cells by direct cell-cell contact and collected at the different time points. Furthermore, BCBL-1 cells were cultured in the conditioned medium that were the normal and heat-inactivatd supernatants of HHV6-infected JJhan cells and also collected at the different time points. Finally, HHV6 viral particles obtained were purified by centrifugation and further be used to infect BCBL-1 cells.Simultaneously, BCBL-1 cells were infected with heat-inactivated or UV-irradiated HHV6 viral particles as control. RT-PCR and/or Real-time quantitative PCR were carried out to evaluate the expression of ORF26 mRNA(encoding the minor capsid protein)of Kaposi's sarcoma-associated herpesvirus(KSHV)in above collected BCBL-1 cells. The results were shown as follows: ①Cytopathogenic effect was observed 15h after cell fusion.ORF26 mRNA expression in BCBL-1 cells detected with RT-PCR increased as significantly compared to that of the control.The results from Real-time PCR analysis at different time points demonstrated that ORF26 mRNA expression in BCBL-1 cells fused with HHV6-infected JJhan cells increased more than 2.3-fold when compared with that in BCBL-1 cells fused with normal JJhan cells. ②ORF26 mRNA expression in BCBL-1 cells cocultured with HHV6-infected JJhan cells increased 1.8-fold at 72h as compared to that of BCBL-1 cells cocul-tured with uninfected JJhan cells.Expression of lytic cycle protein K8.1 in BCBL-1 cells cocultured with HHV6-infected JJhan cells increased 2.46-fold at the 5th day as compared to that of the control. ③ORF26 mRNA expression in BCBL-1 cells cultured for 96 hours in conditioned medium whinh came from the normal and heat-inactivated supernatants of HHV6-infected JJhan cells increased 2.73 and 2.22-fold respectively as compared to that of the relative controls. ④Both alive and inactivated HHV6 viral particles may enhance ORF26 mRNA transcription of KSHV in BCBL-1 cells.These results suggested that HHV6 infection could activate KSHV lytic cycle replication.

运用细胞融合、细胞混合培养、条件培养基培养和病毒直接刺激等方法,研究人类疱疹病毒6型(HHV6)对卡波济肉瘤相关疱疹病毒(KSHV)溶解性周期复制的影响。①将HHV6感染的JJhan细胞(T淋巴细胞系)与BCBL-1细胞(原发性渗出性淋巴瘤,PEL)进行细胞融合形成异核体细胞。②将HHV6感染的JJhan细胞与BCBL-1细胞进行混合培养。③收集HHV6感染的JJhan细胞培养上清液作为条件培养基进行灭活处理,以灭活前后的条件培养基培养BCBL-1细胞。进一步离心纯化HHV6病毒颗粒,并感染BCBL-1细胞,分别设紫外线和热灭活的HHV6病毒颗粒感染BCBL-1细胞为对照。提取上述的实验细胞总RNA,RT-PCR和/或实时定量(Real—time)PCR检测卡波济肉瘤相关疱疹病毒(KSHV)次要衣壳蛋白编码基因ORF26mRNA转录。结果显示:①细胞融合后15h开始出现明显细胞病变,RT-PCR检测不同时间的实验组ORF26mRNA转录水平均明显高于对照组;Real—timePCR检测各时间ORF26mRNA转录水平是对照组的2.3倍以上;②细胞混合培养72h时,实验组ORF26mRNA转录水平是...

运用细胞融合、细胞混合培养、条件培养基培养和病毒直接刺激等方法,研究人类疱疹病毒6型(HHV6)对卡波济肉瘤相关疱疹病毒(KSHV)溶解性周期复制的影响。①将HHV6感染的JJhan细胞(T淋巴细胞系)与BCBL-1细胞(原发性渗出性淋巴瘤,PEL)进行细胞融合形成异核体细胞。②将HHV6感染的JJhan细胞与BCBL-1细胞进行混合培养。③收集HHV6感染的JJhan细胞培养上清液作为条件培养基进行灭活处理,以灭活前后的条件培养基培养BCBL-1细胞。进一步离心纯化HHV6病毒颗粒,并感染BCBL-1细胞,分别设紫外线和热灭活的HHV6病毒颗粒感染BCBL-1细胞为对照。提取上述的实验细胞总RNA,RT-PCR和/或实时定量(Real—time)PCR检测卡波济肉瘤相关疱疹病毒(KSHV)次要衣壳蛋白编码基因ORF26mRNA转录。结果显示:①细胞融合后15h开始出现明显细胞病变,RT-PCR检测不同时间的实验组ORF26mRNA转录水平均明显高于对照组;Real—timePCR检测各时间ORF26mRNA转录水平是对照组的2.3倍以上;②细胞混合培养72h时,实验组ORF26mRNA转录水平是对照组的1.8倍;混合培养5天时,实验组KSHV裂解周期蛋白K8.1表达水平是对照组的2.46倍;③灭活前后的HHV6感染细胞培养上清液培养BCBL-1细胞96h时,ORF26mRNA转录水平分别是对照组的2.73倍和2.22倍;④灭活前后的HHV6均可增强BCBL-1细胞中KSHVORF26mRNA转录水平。提示:HHV6感染可激活KSHV的溶解性周期复制。

Objective To determine whether HIV-1-related inflammatory cytokines can activate lytic cycle replication of Kaposi′s sarcoma-associated herpesvirus(KSHV) in human umbilical vein endothelial cells(HUVEC) and to investigate its potential role in Rta promoter of KSHV involved in reactivation. Methods Using an in vitro model system, we examined the effect of recombinant human cytokines that were similar to that produced by HIV-1-infected T cells on KSHV replication in latently infected HUVEC. KSHV reactivation was...

Objective To determine whether HIV-1-related inflammatory cytokines can activate lytic cycle replication of Kaposi′s sarcoma-associated herpesvirus(KSHV) in human umbilical vein endothelial cells(HUVEC) and to investigate its potential role in Rta promoter of KSHV involved in reactivation. Methods Using an in vitro model system, we examined the effect of recombinant human cytokines that were similar to that produced by HIV-1-infected T cells on KSHV replication in latently infected HUVEC. KSHV reactivation was analyzed with Northern blot analysis and quantitative PCR for ORF26 mRNA expression, a gene encoding the KSHV minor capsid protein was only activated during reactivation. The results were extended and confirmed using a luciferase reporter construct driven by the KSHV Rta(replication and transcription activator) promoter, the first promoter activated during KSHV replication. Results Recombinant cytokines, such as gamma interferon(IFN-γ), hepatocyte growth factor/scatter factor (HGF/SF), and oncostatin M(OSM) not only induce KSHV lytic cycle replication, but also stimulate Rta promoter activity in latently infected HUVECs. Conclusion HIV-1-related inflammatory cytokines are responsible for the induction of KSHV lytic cycle replication in HUVEC and the induction may be, at least in part, mediated by Rta promoter of KSHV.

目的研究HIV21感染相关炎症细胞因子是否能够激活人脐静脉血管内皮细胞(HUVEC)中潜伏感染的卡波济肉瘤相关疱疹病毒(KSHV);探讨该激活作用是否由KSHVRta基因所介导。方法采用体外模拟系统,研究了与HIV-1感染T细胞诱生的细胞因子相似的重组人细胞因子对HUVEC中潜伏感染的KSHV复制的影响。通过Northernblot和定量PCR检测ORF26(该基因编码的病毒次要衣壳蛋白仅在KSHV被激活时表达)mRNA表达来分析KSHV的激活。运用KSHVRta基因启动子(KSHV复制时最先被激活的启动子)驱动的虫荧光素酶报告基因进一步证实并扩展研究结果。结果包括干扰素-γ(IFN2γ)、肝细胞生长因子P扩散因子(HGFPSF)及制瘤蛋白M(oncostatinM,OSM)在内的重组细胞因子不仅可诱导HUVEC中潜伏感染的KSHV发生溶解性周期复制,而且能增强Rta启动子活性。结论HIV-1感染相关的炎性细胞因子是诱导HUVEC中KSHV溶解性周期复制的因素,而且该过程至少有部分由KSHV的Rta启动子介导。

 
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