while methicillin-resistant Staphylococcus aureus(MRSA) accounted for 7.3% of S.aurens strains. High-level aminoglycoside resistant(HLAR) strains accounted for 79.6% and 63.9% in strains of E.
Genotyping was carried with random amplified polymorphic DNA (RAPD). RESULTS The rates of ARE, HLAR, VRE and ARE & HLAR were 17.0%, 38.2%, 0% and 5.4%, respectively. The rate of VIE was 2.1%.
CONCLUSIONS The study indicated that in Xiangfan area, Hubei Province the essential mechanisms of aminoglycoside resistance in P. aeruginosa result in producing aminoglycosides modifying enzyme (57 1%). In addition, in this group 15 strains are resistant to aminoglycosides but the nine types of genes are negative, the other modifying enzyme gene is possible or 16S rRNA mutation occurred.
Results The ESBLs-producing organisms were 36 strains from 215 strains detected. The ratio was 16.7%. The resistance rate for 12 antibiotics in positive ESBLs was much higher than that of in negtive while it was multiply resistance for Quinolones and Aminoglylosides antibiotic.
The drug-resistance rate of ESBLs-producing trains to common antimicrobials was obviously higher than that of non-ESBLs-producing train and the former also resisted the aminoglycosides and quinolones.
Methods The nucleotide sequence of aminoglycoside-modifying enzyme genes in ESBLs-producing plus aminoglycoside-resistant strains amplified by PCR were detected by(automatic) sequencer,and compared with relative DNA sequencing reported by others in Internet GenBank through Blastx searching.
In the present study, the relationship between the lethal cytotoxic effect of aminoglycoside and its effect on apical membrane enzyme was examined by establishing aminoglycoside resistant cells.
Aim To understand the detective methods and resistance of ESBLs-producing organisms.Methods To detect the extended spectrum β-lactancases of Klebsiella Pneumonias and E.Coli of clinical specimens and test the susceptibility by Vitek AMS and GNS-NT and GNS-F2 cards.Results The ESBLs-producing organisms were 36 strains from 215 strains detected. The ratio was 16.7%.The resistance rate for 12 antibiotics in positive ESBLs was much higher than that of in negtive while it was multiply resistance for Quinolone...
Eighty eight clinical isolates of Enterococcus spp. in our hospital were collected and their resistance to antibiotics as well as the relationship between the pathogens and the incidence of infectious diseases were also investigated. The identification and the drug susceptibility tests were analyzed by VITEK automatic microbiology analytical instrument system. Rusults showed that the resistant rates of infections due to Enterococcus spp. to nitrofurantion and vancomycin were 1.5% and 2.3% respectiv...
Objective To understand the current drug resistance of Klebsiella pneumoniae in lower respiratory tract infection and the prevalence trend of extended spectrum beta lactamase (ESBLs) producing Klebsiella pneumoniae Methods Drug resistance in 86 strains of Klebsiella pneumoniae and ESBLs producing strains were genotyped by randomly amplified DNA polymorphisms (RAPD) Results ESBLs producing strains accounted for 20 9% of the 86 strains, and 83 3% of the ESBLs producing strains were f...