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粘附素基因
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  adhesin gene
     Prokaryotic Expression of P30 Adhesin Gene of Mycoplasma Pneumoniae and Preliminary Application of the rP30 Protein
     肺炎支原体P30粘附素基因的原核表达和初步应用
短句来源
     Research on an Adhesin Gene hpaA of Helicobacter pylori
     幽门螺杆菌粘附素基因hpaA研究
短句来源
     Cloning and sequence analysis of adhesin gene hpaA of Helicobacter pylori
     幽门螺杆菌粘附素基因hpaA的克隆及序列分析
短句来源
     Aim To clone and sequence adhesin gene papG obtained from uropathogenic E. coli strain 132 (UPEC132).
     目的 克隆F13型致肾盂肾炎大肠杆菌 (UPEC) 132株的粘附素基因papG并作序列分析。
短句来源
     PART I Prokaryotic expession of P30 adhesin gene ofMycoplasma pneumoniae and preliminary analysis of the rP30proteinObjective: To construct the prokaryotic expression vector carrying intactP30 gene and express P30 in E.
     第一部分 肺炎支原体 P30 蛋白的原核表达及初步分析目的:通过体外扩增 MP 的 P30 粘附素基因,构建 P30 基因的原核表达质粒,在 E. coli 中表达并鉴定;
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  “粘附素基因”译为未确定词的双语例句
     In the present study, the whole genome sequence of biofilm negative S. epidermidis strain ATCC 12228 was analyzed and compared with that of biofilm positive S. epidermidis strain RP62A and that of Staphylococcus aureus strain N315 to identify biofilm related genes.
     本文以对生物膜阴性的表皮葡萄球菌ATCC 12228进行全基因组测序为基础,通过其与金黄色葡萄球菌N315,生物膜阳性表皮葡萄球菌RP62A进行全基因组比较,分析了表皮葡萄球菌生物膜相关基因,重点研究了细胞间粘附素基因(ica)的功能,并对其表达调节进行了初步研究。
短句来源
     Cloning and expression of adhesion gene hpaA of Helicobacter pylori
     幽门螺杆菌粘附素基因hpaA的克隆及表达
短句来源
     Prokaryotic Expression of Hpa A Gene of Helicobacter Pylori
     幽门螺杆菌粘附素基因的原核表达
短句来源
     Conclusion The C→160a promoter polymorphism of E-cadherin gene may not play a major role in the etiology of non-cardia gastric cancer.
     结论E-钙粘附素基因(CDH1)启动子区-160C→A多态性与胃癌遗传易感性不相关。
短句来源
     Identification of the Putative Cytadhesin Gene of Mycoplasma Gallisepticum and Its Use as a DNA Probe
     鸡毒支原体的假定细胞粘附素基因鉴定及用作DNA探针的研究
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  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Research on an Adhesin Gene hpaA of Helicobacter pylori
     幽门螺杆菌粘附素基因hpaA研究
短句来源
     Prokaryotic Expression of Hpa A Gene of Helicobacter Pylori
     幽门螺杆菌粘附素基因的原核表达
短句来源
     Overexpression of AK fbr gene in C.
     AKfbr基因在C.
短句来源
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  adhesin gene
Similar results were obtained with an S-pili determinant which mediates binding to sialic acid-containing receptors and the findings suggest that the regulatory regions may be more conserved than other genes in different pili-adhesin gene clusters.
      
In strains expressing more than one kind of pili the trans-active gene products thereby may allow for regulatory interaction between separate pili-adhesin gene systems.
      
The Prevotella loescheii adhesin gene, plaA, contains a coding gap between a small open reading frame (ORF-1) and a large open reading frame (ORF-2).
      
DNA sequence analysis of the regions surrounding the Mycoplasma hyopneumoniae P97 swine ciliary adhesin gene.
      
In some cases evolution towards a single niche or host has been driven by or resulted in the silencing of certain adhesin gene clusters.
      
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In order to investigate the P-pilus adhesion serotypes and lay a foundation of the clinical identification of uropathogenic E. coli strains. Methods: Using the clone strains carrying the adhesive gene cluster of six P-pilus adhesion se- rotypes from the uropathogenic E. coli(UPEC)treated with formalin, the antisera were prepared by intravenously injecting UPEC clone strains into rabbits, respectively. After absortion the antisera were only specific against the adhesive gene cluster. The whole-bacterium ELISA...

In order to investigate the P-pilus adhesion serotypes and lay a foundation of the clinical identification of uropathogenic E. coli strains. Methods: Using the clone strains carrying the adhesive gene cluster of six P-pilus adhesion se- rotypes from the uropathogenic E. coli(UPEC)treated with formalin, the antisera were prepared by intravenously injecting UPEC clone strains into rabbits, respectively. After absortion the antisera were only specific against the adhesive gene cluster. The whole-bacterium ELISA was carried out with the single or multiple valence antisera and compared with the hemagglutination test for 95 strains of E. coli isolated from urine. Results: It was showed that the positive strains with whole-bacterium ELISA examination among MRHA≥ + + and negative tested stains were 37.3% (25/67) and 3.6% (1/28),respectively. 88% of the former were MRHA+ + + ~ + + + +. Among 26 strains expressing positive in whole- bacterium ELISA, F13 and F7 serotypes of P-pilus adhesion were 88. 5% and 11. 5%, respectively. Conclusion:The whole- bacterium ELISA with F13 serotype or multiple valence antisera could be used for identification of UPEC strains, and it was better than the hemagglutination test.

研究致肾盂肾炎大肠杆菌P菌毛粘附素血清型,为该菌的临床鉴定奠定基础。方法:利用6个带有致肾盂肾炎大肠杆菌不同血清型P菌毛粘附素基因群的克隆株免疫新西兰白兔获得抗血清,经吸收后仅保留对各血清型粘附基因群的特异性。采用单价和多价抗血清建立了全菌ELISA方法,用于95株尿源性大肠杆菌的研究,并与血凝试验进行比较。结果:95株受检菌株中,MRHA阳性菌株中37.3%(25/67株)全菌ELISA阳性,其中MRHA+++~++++菌株占 88.0%(22/25)株,MRHA阴性菌株中全菌ELISA阳性者仅为 3.6%(1/28)株。26株全菌ELISA阳性菌P菌毛粘附素,F13型为88.5%,F7型为11.5%。结论:采用F13型或多价抗血清建立的全菌ELISA方法可用于UPEC的鉴定。

Objective To show the virulence related to the

目的揭示抗生素诱变的球形幽门螺杆菌(Hp)与定居有关毒力的变异情况。方法采用亚抑菌浓度抗生素作用,使5株Hp发生球形变异,检测球形Hp的尿素酶活性及其对Hep2细胞(喉癌上皮细胞)的粘附性,并用PCR法检测其尿素酶A,尿素酶B及粘附素基因。结果球形Hp尿素酶活性降低,对Hep2细胞的粘附性降低,电镜下可见球形Hp侵入细胞内。球形Hp的411bp尿素酶A,115bp尿素酶B及375bp粘附素基因的PCR均阳性。结论抗生素诱变的球形Hp与定居相关的毒力减弱,但其有关毒力基因片段未缺失。

A Portion of the putative cytadhesin Gene of Mycoplasma gallisepticum (MG) was identified and used as a probe. A pair of degenerate oligonucleotide primers named L2R2were synthesized according to conserved regions of the cytadhesin proteins from M. pneumonias. MG-S6DNA were used as model in L2R2 primered PCR. A 854bp DNA fragment was amplified and coffeed horn gel. Then it was cloned and sequenced. Homogenious analysis showed it had significantly consistence with its counterpart of M. pneumonia The amplified...

A Portion of the putative cytadhesin Gene of Mycoplasma gallisepticum (MG) was identified and used as a probe. A pair of degenerate oligonucleotide primers named L2R2were synthesized according to conserved regions of the cytadhesin proteins from M. pneumonias. MG-S6DNA were used as model in L2R2 primered PCR. A 854bp DNA fragment was amplified and coffeed horn gel. Then it was cloned and sequenced. Homogenious analysis showed it had significantly consistence with its counterpart of M. pneumonia The amplified 854bp DNA fragment was labelled with DIG and used as a diagnostic probe iD study its distribution among other specices of Mycoplasmas. The results showed that it could hybridize with all five MG DNA rather than M. synoviae and M. gallinarum.

鉴定了鸡毒支原体的假定细胞粘附素基因的一部分,并将其用作DNA诊断探针。根据肺炎支原体细胞粘附素蛋白质的基因保守区两侧序列,设计并合成一对变性寡核苷酸引物L2R2,用于以鸡毒支原体S6株基因组DNA为模板的PCR反应。扩增获得一个长854bp的DNA片段,随后进行了克隆和测序。同源性分析表明扩增片段与肺炎支原体细胞粘附素基因相应部分具有显著的一致性。回收鸡毒支原体假定细胞粘附素基因扩增片段,制成地布辛标记探针,对这个片段在3种禽支原体中的分布进行研究发现,该PCR产物可与本试验所有的5个鸡毒支原体菌株基因组DNA杂交;不能与滑液支原体、家禽支原体DNA杂交。

 
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