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   粘附素基因 在 生物学 分类中 的翻译结果: 查询用时:0.091秒
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  adhesin gene
    Cloning and sequence analysis of adhesin gene hpaA of Helicobacter pylori
    幽门螺杆菌粘附素基因hpaA的克隆及序列分析
短句来源
    Prokaryotic Expression of P30 Adhesin Gene of Mycoplasma Pneumoniae and Preliminary Application of the rP30 Protein
    肺炎支原体P30粘附素基因的原核表达和初步应用
短句来源
    PART I Prokaryotic expession of P30 adhesin gene ofMycoplasma pneumoniae and preliminary analysis of the rP30proteinObjective: To construct the prokaryotic expression vector carrying intactP30 gene and express P30 in E.
    第一部分 肺炎支原体 P30 蛋白的原核表达及初步分析目的:通过体外扩增 MP 的 P30 粘附素基因,构建 P30 基因的原核表达质粒,在 E. coli 中表达并鉴定;
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  “粘附素基因”译为未确定词的双语例句
    Identification of the Putative Cytadhesin Gene of Mycoplasma Gallisepticum and Its Use as a DNA Probe
    鸡毒支原体的假定细胞粘附素基因鉴定及用作DNA探针的研究
短句来源
    Prokaryotic Expression of Hpa A Gene of Helicobacter Pylori
    幽门螺杆菌粘附素基因的原核表达
短句来源
    In the present study, the whole genome sequence of biofilm negative S. epidermidis strain ATCC 12228 was analyzed and compared with that of biofilm positive S. epidermidis strain RP62A and that of Staphylococcus aureus strain N315 to identify biofilm related genes.
    本文以对生物膜阴性的表皮葡萄球菌ATCC 12228进行全基因组测序为基础,通过其与金黄色葡萄球菌N315,生物膜阳性表皮葡萄球菌RP62A进行全基因组比较,分析了表皮葡萄球菌生物膜相关基因,重点研究了细胞间粘附素基因(ica)的功能,并对其表达调节进行了初步研究。
短句来源
    ObjectiveTo detect the frequency of I type fimbria and analyse the sequence of FimH gene of uropathogenic Escherichia coli(UPEC) clinical isolates.
    目的检测尿路致病性大肠杆菌 (uropathogenicEscherichiacoli,UPEC)临床株I型菌毛的携带频率 ,并对其粘附素基因FimH作序列分析。
短句来源
    The Function and Regulation of Intercellular Adhesin (ica) Genes in Staphylococcus Epidermidis
    表皮葡萄球菌细胞间粘附素基因功能及表达调控的研究
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Objective To show the virulence related to the

目的揭示抗生素诱变的球形幽门螺杆菌(Hp)与定居有关毒力的变异情况。方法采用亚抑菌浓度抗生素作用,使5株Hp发生球形变异,检测球形Hp的尿素酶活性及其对Hep2细胞(喉癌上皮细胞)的粘附性,并用PCR法检测其尿素酶A,尿素酶B及粘附素基因。结果球形Hp尿素酶活性降低,对Hep2细胞的粘附性降低,电镜下可见球形Hp侵入细胞内。球形Hp的411bp尿素酶A,115bp尿素酶B及375bp粘附素基因的PCR均阳性。结论抗生素诱变的球形Hp与定居相关的毒力减弱,但其有关毒力基因片段未缺失。

A Portion of the putative cytadhesin Gene of Mycoplasma gallisepticum (MG) was identified and used as a probe. A pair of degenerate oligonucleotide primers named L2R2were synthesized according to conserved regions of the cytadhesin proteins from M. pneumonias. MG-S6DNA were used as model in L2R2 primered PCR. A 854bp DNA fragment was amplified and coffeed horn gel. Then it was cloned and sequenced. Homogenious analysis showed it had significantly consistence with its counterpart of M. pneumonia The amplified...

A Portion of the putative cytadhesin Gene of Mycoplasma gallisepticum (MG) was identified and used as a probe. A pair of degenerate oligonucleotide primers named L2R2were synthesized according to conserved regions of the cytadhesin proteins from M. pneumonias. MG-S6DNA were used as model in L2R2 primered PCR. A 854bp DNA fragment was amplified and coffeed horn gel. Then it was cloned and sequenced. Homogenious analysis showed it had significantly consistence with its counterpart of M. pneumonia The amplified 854bp DNA fragment was labelled with DIG and used as a diagnostic probe iD study its distribution among other specices of Mycoplasmas. The results showed that it could hybridize with all five MG DNA rather than M. synoviae and M. gallinarum.

鉴定了鸡毒支原体的假定细胞粘附素基因的一部分,并将其用作DNA诊断探针。根据肺炎支原体细胞粘附素蛋白质的基因保守区两侧序列,设计并合成一对变性寡核苷酸引物L2R2,用于以鸡毒支原体S6株基因组DNA为模板的PCR反应。扩增获得一个长854bp的DNA片段,随后进行了克隆和测序。同源性分析表明扩增片段与肺炎支原体细胞粘附素基因相应部分具有显著的一致性。回收鸡毒支原体假定细胞粘附素基因扩增片段,制成地布辛标记探针,对这个片段在3种禽支原体中的分布进行研究发现,该PCR产物可与本试验所有的5个鸡毒支原体菌株基因组DNA杂交;不能与滑液支原体、家禽支原体DNA杂交。

Objective: To study the molecular mechanism of the adhesion of Helicobacter pylori. Methods: The special primers of adhesin gene hpaA of H.pylori were designed and a polymerase chain reaction was performed. Then PCR products were inserted into vector pUC19 and sequenced. Results: A recombinant plasmid pUC hpa was constructed. DNA sequencing showed one open reading frame of 783 bp, which encoded polypeptides of 261 amino acids. The HpaA contains a short sequence of amino acids(KRTIQK) which are similar to those...

Objective: To study the molecular mechanism of the adhesion of Helicobacter pylori. Methods: The special primers of adhesin gene hpaA of H.pylori were designed and a polymerase chain reaction was performed. Then PCR products were inserted into vector pUC19 and sequenced. Results: A recombinant plasmid pUC hpa was constructed. DNA sequencing showed one open reading frame of 783 bp, which encoded polypeptides of 261 amino acids. The HpaA contains a short sequence of amino acids(KRTIQK) which are similar to those which compose the sialic acid binding motif of E.coli adhesins K99, SfaS and CFA/I. Conclusion: The hpaA gene has differences in sequences between H.pylori strains. But they have similar molecular basis for adhesion to host cells. [

对一株临床分离的高粘附力幽门螺杆菌 (Helicobacterpylori ,Hp)菌株M 1进行粘附素基因hpaA的序列分析 ,为研究Hp的粘附提供分子基础。 方法 :设计hpaA的特异引物 ,将PCR产物插入pUC19载体构建hpaA的重组克隆 ,DNA自动分析仪进行序列测定 ,ClustalX和Homology软件分析DNA及其推导出的氨基酸序列。 结果 :构建了粘附素HpaA的重组质粒 pUC hpa ,测序显示hpaA结构基因长 783bp ,开放读框完整 ,无中断 ,与文献报道的序列有差异 ;该基因编码 2 61个氨基酸 ,其中一段氨基酸序列KRTIQK与大肠杆菌粘附素K99、SfaS、CFA/Ⅰ中的涎酸结合位点的结构相似。结论 :Hp粘附素基因hpaA在不同菌株存在差异 ,但其粘附作用有相似的分子基础。

 
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