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即刻早期
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  immediate-early
     Analysis of siRNA Interfering in the α22 Immediate-early Gene of HSV-1
     SiRNA干扰HSV-1即刻早期基因α22效应的初步分析
短句来源
     Expression of immediate-early genes c-fos and c-jun in the marginal division of striatum during learning and memory
     学习记忆过程中即刻早期基因c-fos、c-jun在纹状体边缘区的表达
短句来源
     Aim To investigate the changes in the expression of protein kinase C gamma (PKCγ), phosphorylated cAMP response element binding protein(pCREB)and immediate-early gene(c-fos and c-jun) in the spinal cord in formalin-induced inflammatory pain and study the effect of agmatine on the changes of PKCγ activation, phosphorylation of CREB and expression of c-fos and c-jun.
     目的研究福尔马林致炎性疼痛脊髓蛋白激酶Cγ(PKCγ),磷酸化的环磷酸腺苷反应元件结合蛋白(pCREB),即刻早期基因c-fos和c-jun表达的变化及胍丁胺对其的影响。
短句来源
     It is one of immediate-early gene indispensable to cell proliferation, and its product is a trans-acting transcription factor that drives the cells from G_0/G_1 phase into S phase.
     它是细胞增殖必不可少的即刻早期基因,是一种调控基因转录的反式作用因子,其表达产物是细胞增殖信号转导的必需因子,也是细胞从G_0/G_1期进入S期的驱动因子。
短句来源
     In this study, α22, an immediate-early gene of HSV-1 was analyzed with RNAi technology. Two sequences of siRNAs (siRNA-1 and siRNA-2) were prepared by in vitro transcription, and transfected into the Hep-2 and KMB17 cells.
     以HSV1即刻早期基因α22为靶点,应用T7RNA聚合酶体外合成siRNA1和siRNA2序列,脂质体转染法将其导入Hep2或KMB17细胞,再接种HSV1病毒,通过细胞形态和病毒滴度的测定,初步探索了siRNA干扰α22基因的的效应。
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  “即刻早期”译为未确定词的双语例句
     Methods The blood samples from 44 SLE patients and 43 matched normal controls were tested for BamHⅠ-W, the specific DNA fragment of EBV, by polymerase chain reaction(PCR)-Southern analysis. RT-PCR and Southern blotting were used to detect the EBV lytic genes (including immediate early genes BZLF1 and BRLF1, early genes BARF1, late genes BcLF1 and BLLF1) in EBV DNA-positive SLE patients.
     方法PCR-Southern印迹检测44例SLE患者和43例正常对照外周血单一核细胞中EBV特异性DNA片段BamHⅠ-W,对EBV阳性标本进行RT-PCR和Southern印迹,检测即刻早期基因BZLF1、BRLF1,早期基因BARF1,晚期基因BcLF1和BLLF1的表达。
短句来源
     RT-PCR and Southern blotting were used to detect the expression of EBV lytic genes (immediately early genes BZLF1 and BRLF1, early genes BARF1 and BHRF1, late genes BcLF1 and BLLF1) in EBV-positive gastric carcinomas.
     再应用RT PCR和Southern杂交技术检测EBV增殖期基因 (即刻早期基因BZLF1、BRLF1,早期基因BARF1、BHRF1,晚期基因BcLF1、BLLF1)的表达。
短句来源
     Construction and Expression of Recombinant Adenoviral Vector Carrying EBV Immediately Early Gene BZLF1
     EBV即刻早期基因BZLF1腺病毒载体的构建和表达
短句来源
     Effect of Methylmercury on Expression of Immediate Early Gene c-jun mRNA in Rat Brain
     甲基汞对大鼠脑即刻早期基因c-jun mRNA表达影响
短句来源
     A plasmid pGL2Rz has been constructed, which can express a triplet ribozyme R426 in Bm cells. R426 contains three hammerhead ribozymes (R47, R208 and R687) targeled to three different sites of BmNPVIE mRNA.
     用自行设计的能专一切割家蚕核多角体病毒即刻早期蛋白(BmNPVIE)mRNA上三个位点的三联体ribozyme-R426(由R47,R208和R687三个ribozyme串联而成),构建了在家蚕细胞中瞬时表达的质粒pGL2Rz。
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  相似匹配句对
     EXPRESSION OF HCMV IMMEDIATE EARLY GENE IN E.coli. DH5α
     HCMV即刻早期基因在大肠杆菌中的表达
短句来源
     Teh immediate-early gene c-fos and opilepsy
     即刻早期基因c-fos与癫
短句来源
     Early Osteoporosis
     早期骨质疏松
短句来源
     PREMATURE MENOPAUSE
     早期绝经
短句来源
     IMMEDIATE IMPLANT
     即刻种植义齿
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  immediate-early
Transcriptional activation of immediate-early genes c-fos, c-jun, and Egr-1 in response to anisomycin treatment of cells transfo
      
The antibiotic anisomycin, a well-known stress factor, has been found to induce the transcription of immediate-early gene c-fos, which is under negative control in cells transformed by oncogenes E1A and cHa-ras.
      
The c-fos gene activation is short-term: it reaches its maximum 1 h after anisomycin treatment, and then the transcription level decreases, which is characteristic of immediate-early genes.
      
The transcription levels of two other immediate-early genes, c-jun and Egr-1, are already relatively high in the absence of anisomycin; however, anisomycin treatment further increases them.
      
Analysis of HSV-I ICP22 effects on HCMV major immediate-early promoter structure
      
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hybridoma cell lines, namely 1E6. 2D8. 5D10. 6F10. 7B4.7B6.7D7. 7E7.7E11. 7F5. 8B8. 8D6 and 8F9 which secreted monoclonal antibodies (McAbs)to human cytomegalovirus strain AD196 (HCMV AD169), were established by fusion of BALB/c mouse rnyeloma Sp2/0 cells with spleen cells of mice immunized with HCMV AD169. 6 hybridoma cell lines (7B4. 7D7. 7E7. 7E11. 8B8. 8D6) were selected for further study.Immunoblot analysis revealed that McAbs 7B4 1 7D7.7E7.7E11 .8B8 and 8D6 are directed against different HCMV specific...

hybridoma cell lines, namely 1E6. 2D8. 5D10. 6F10. 7B4.7B6.7D7. 7E7.7E11. 7F5. 8B8. 8D6 and 8F9 which secreted monoclonal antibodies (McAbs)to human cytomegalovirus strain AD196 (HCMV AD169), were established by fusion of BALB/c mouse rnyeloma Sp2/0 cells with spleen cells of mice immunized with HCMV AD169. 6 hybridoma cell lines (7B4. 7D7. 7E7. 7E11. 8B8. 8D6) were selected for further study.Immunoblot analysis revealed that McAbs 7B4 1 7D7.7E7.7E11 .8B8 and 8D6 are directed against different HCMV specific polypeptides with molecular weights of approximately 46, 150, 38, 51, 72 and 65kD respeetively. Indirect immunofluorescence reactions of McAbs with HCMV-infected cells at different time showed that McAb 8B8 was against the immediate early antigen of HCMV, while McAbs 7B4 .7D7.7E7.7E11 and 8D6 were against the late antigens of HCMV. A pool of the 6 McAbs conjugated to horseradish peroxidase was used in IgM antibody capture ELISA (MacELISA) for detection of HCMY-SIgM. The method was compared with indirect ELISA (IELISA). 100 unselected cord serum specimens were tested with MacELISA and IELISA simultaneously. 3specimens were positive by both methods, while 94 specimens were negative by both assays. 3 discordant positive specimens were evaluated by specific tests. 2 positive specimens by MacELISA while negative by IELISA were proyed to be HCMV-SIgh positive. 1 positive specimen by IELISA while negative by MacELISA was proved to be false positive caused by RF. The results indicated that MacELISA had higher specificity and sensitivity. So the method could be applied for detection of HCMV-SIgM in clinical laboratories.

采用人巨细胞病毒(HCMV)AD169株作为免疫原,制备出13株鼠-鼠杂交瘤细胞系。对其中的6株进行了检定.免疫印迹试验结果表明:单克隆抗体(McAb)7B4、7D7、7E11、8E8和8D6相对应的HCMV多肽分子量分别为46、150、38、5172和65kD.HCMV感染人胚肺二倍体细胞(2BS)后不同时间制成抗原片,与McAb作间接免疫荧光试验。结果表明:McAb8B8相应的病毒多肽为即刻早期抗原,其它5株McAb相应的病毒多肽均为晚期抗原,6株McAb等量混合后,标上辣根过氧化物酶,用于IgM抗体捕获法ELISA(MacELISA)中,并与间接ELISA(IELISA)同时检测HCMV-IgM.在未经选择的100份脐带血中,两法均为阳性的3份,两法均为阴性的94份;MacELISA阳性而IELISA阴性的2份血清的特异性试验证明,HCMV-IgM确为阳性.IELISA阳性而MacELISA阴性的1份血清的特异性试验证明,它是由RF引起的假阳性。

The Fos protein derived from c-fos proto-oncogene has been known to form a heterodimer with Jun protein, which functions as a third messenger to indece the expression of target gene. Immunohistochemical technique was used in the present study to detect and compare Fos and Jun expressed in adjacent sections of the rat superior colliculus (SC) following electroacupuncture (EA) stimulation (2Hz, 1 - 2 - 3 mA, 30min). It was shown that the expression of Fos and Jun in SC was scaty in normal animals. EA stimulatin...

The Fos protein derived from c-fos proto-oncogene has been known to form a heterodimer with Jun protein, which functions as a third messenger to indece the expression of target gene. Immunohistochemical technique was used in the present study to detect and compare Fos and Jun expressed in adjacent sections of the rat superior colliculus (SC) following electroacupuncture (EA) stimulation (2Hz, 1 - 2 - 3 mA, 30min). It was shown that the expression of Fos and Jun in SC was scaty in normal animals. EA stimulatin induced a 10 fold inerease in Fos expression and 18 fold increase in Jun expression. The distribution patterns for the two proteins in SC were similar, with the heaviet labeling found in the superficial layer, moderate in the intermediate layer and the lightest in the deep layer. The number of Jun-like immunoreactive neurons doubled that of Fos - like immunoreactive neurons in all levels and subdivisions of SC. SC has been known to be functionally related to vision, although recent studies suggest it may also be involved in nociception. The functional significance of a dramatic expression of Fos and Jun in SC following EA stimulation remains to be elucidaled.

大量资料表明,即刻早期基因产物Fos蛋白与Jun蛋自的诱导表达可以做为神经元活动的标志物。本研究利用免疫组化技术观察并比较外周电针刺激(2Hz,1—2-3mA,30min)后,Fos、Jun样蛋白在大鼠上丘的表达。结果表明,正常大鼠上丘的Fos、Jun表达很低,而电针可使Fos表达增加10倍,Jun的表达增加18倍。Fos、Jun样免疫反应阳性神经元在上丘备层的分布基本相似,均以表层分布最为密集,中间层次之,深层最少。阳性神经元计数结果表明,Jun在上丘各层次及各断面的表达高出Fos约一倍。关于电针刺激上丘表层大量Fos、Jun表达功能意义有待进一步研究。

Using the techniques of immunohistochemistry,Northern blotting and in situ hybridization,we have found that electroacupuncture(2/15Hz) accelerated the expression of mRNA and protein synthesis of immediate early genes c-fos,c-jun as well as proenkephalin gene in rat central nervous system(CNS).Northern hybridization revealed that EA stimulation induced c-fos gene expression within 1一2h,followed by PENK gene expression which peaked at 4 8h. Morphological study demonstrated that the sites of c-fos,c-jun and PENK...

Using the techniques of immunohistochemistry,Northern blotting and in situ hybridization,we have found that electroacupuncture(2/15Hz) accelerated the expression of mRNA and protein synthesis of immediate early genes c-fos,c-jun as well as proenkephalin gene in rat central nervous system(CNS).Northern hybridization revealed that EA stimulation induced c-fos gene expression within 1一2h,followed by PENK gene expression which peaked at 4 8h. Morphological study demonstrated that the sites of c-fos,c-jun and PENK mRNA expression and protein synthesis coincide with each other in the CNS.The results suggest that Fos/Jun may function as the transcription regulator of PENK gene.

采用免疫组织化学、Northern杂交与原位杂交等方法,观察到2/15H电针刺激可诱导大鼠中枢神经系统内即刻早期基因c-fos、c-jun的mRNA与免疫阳性物质以及前脑啡肽(PENK)mRNA的生成。Northern杂交显示,电针在1~2h内引起即刻早期基因c-fosmRNA的表达,继之以PENKmRNA的表达,在48h内方达顶点;形态学结果表明,电针诱导c-fos、c-jun与PENKmRNA及其蛋白产物的生成在中枢的分布具有明显的一致性。提示Fos/Jun可能作为PENK基因的转录调节因子。

 
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