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   启动子基因 的翻译结果: 查询用时:0.027秒
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启动子基因
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  promoter gene
     CONCLUSION: Curcumin, with epigenetic modificative effects, could enhance the acetylayion of histone H3 at the site of p21WAF1/CIP1 promoter gene, improve transcription of p21WAF1/CIP1 gene, and arrest cell cycle progression of Raji cells.
     结论:姜黄素通过表观遗传修饰作用,调节p21WAF1/CIP1启动子基因位点组蛋白H3乙酰化水平,促进p21WAF1/CIP1基因转录,阻滞Raji细胞周期进程。
短句来源
     Isolation of DNA from HSV-2 and Purification of ICP10 Promoter Gene
     单纯疱疹病毒2型DNA提取及ICP10启动子基因获得和纯化
短句来源
     The levels of acetylated histone H3 and p21WAF1/CIP1 were detected by Western blot, the expression of p21WAF1/CIP1 gene was detected by reverse transcription-polymerase chain reaction (RT-PCR), and the level of acetylated histone H3 at the site of p21WAF1/CIP1 promoter gene was examined by chromatin immunoprecipitation assay.
     RT-PCR检测p21WAF1/CIP1基因表达; 染色质免疫沉淀分析p21WAF1/CIP1的启动子基因位点组蛋白H3乙酰化水平;
短句来源
     MMP3 promoter gene containing the 5A/6A polymorphism was amplified by polymerase chain reaction(PCR).
     聚合酶链反应( PCR)法扩增 MMP 3启动子区域 ,酶切法及琼胶电泳法对 MMP 3启动子基因 5 A/ 6 A多态性进行分析。
短句来源
     A Relative Study between the MMP-1 Promoter Gene Polymorphism and Periodontitis
     MMP-1启动子基因多态性与牙周炎相关性研究
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  “启动子基因”译为未确定词的双语例句
     Methods: Tomato fruit-specific promoters’ gene 2A12 and E8 were respectively introduced to pBPFΩ7 to form pB2A12 and pBE8. The DNA fragment containing HBsAg-s gene from plasmid YEP-HBs was inserted respectively into pB2A12 and pBE8 to form pB2A12-HBs and pBE8-HBs.
     方法将番茄果实特异性启动子基因2A12和E8 ,分别插入到pBPFΩ7构建中间载体pB2A12和pBE8 ; 酶切YEP-HBs载体中的HBsAg小蛋白,并插入到pB2A12和pBE8 ,得到载体pB2A12-HBs和pBE8-HBs ;
短句来源
     RESULTS: Curcumin induced hyperacetylation of histone H3 at the site of p21WAF1/CIP1 promoter by 1.9 folds, and enhanced the levels of p21WAF1/CIP1 mRNA by 4.2 folds and protein by 5.1 folds 24 h after treatment.
     结果:姜黄素提高p21WAF1/CIP1的启动子基因位点组蛋白H3乙酰化水平1.9倍; 使p21WAF1/CIP1mRNA合成增加和蛋白表达上调,在24h时分别增加了4.2倍和5.1倍;
短句来源
     The Effect of Hepatic Lipase Gene Promoters-250G/A and-514C/T Polymorphism on Plasma Lipoproteins Metabolism and Coronary Heart Disease
     肝脂酶启动子基因-250G/A和-514C/T多态性与血浆脂质代谢和冠心病的关系
短句来源
     A Study on the Association of the Serum Concentration and the Promoter 5A/6A Polymorphism of Matrix Metalloproteinase-3 and the Serum Concentration of Interleukin-1 with Coronary Heart Disease
     基质金属蛋白酶-3及其启动子基因5A/6A多态性、白介素-1与冠心病关系的研究
短句来源
     Polymorphistic Analysis of Paraoxon Promotor Genes-909C/G among Coronary Heart Disease Patients
     冠心病患者对氧磷酶启动子基因-909C/G多态性分析
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  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Cloning of Medicinal Gene and Promoter from Ganoderma
     灵芝药效基因启动子的克隆
短句来源
     Cloning and identification of rod opsin promoter
     视蛋白基因启动子的克隆和鉴定
短句来源
     The Gene of All Fears
     害怕的基因
短句来源
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  promoter gene
GAF acts as a transcriptional antirepressor, i.e., its interaction with nucleosomal DNA results in the open chromatin conformation in promoter gene regions.
      
Furthermore, this influence was shown to be associated with the presence of retrotransposon MDG4 sequences in the pre-promoter gene region.
      
Markers located near the top telomeric region of chromosome 9 showed segregation highly skewed towards the wild allele through all generations, suggesting the presence of a gamete promoter gene.
      
Cauliflower mosaic virus 35S (CaMV-35S) promoter gene, a widespread genetic element, was amplified by a set of LAMP primers.
      
The measurement of the turbidity of the reaction mixture allows easy detection of amplification of CaMV-35S promoter gene without the need for gel electrophoresis.
      
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The peptide corresponding to the 141-160 sequences of VP1 of foot and mouth disease virus is an immunogenic domain .It contains 20 amino acids. The gene fragment coding for this immunogenic peptide was chemically sy-thesized by DNA synthesizer. This fragment is 72bp long with Eco RI sites at the 5' end and a Bam HI sites at the 3' end.The synthetic nucleotide fragment was inserted into plasmid pWR590 which is an expression vector with lac promoter. Identification of the gene product was carried oat by using...

The peptide corresponding to the 141-160 sequences of VP1 of foot and mouth disease virus is an immunogenic domain .It contains 20 amino acids. The gene fragment coding for this immunogenic peptide was chemically sy-thesized by DNA synthesizer. This fragment is 72bp long with Eco RI sites at the 5' end and a Bam HI sites at the 3' end.The synthetic nucleotide fragment was inserted into plasmid pWR590 which is an expression vector with lac promoter. Identification of the gene product was carried oat by using the antibody of O type of foot and mouth disease virus. 8 clones from 210 transformants were positive. The expressed product formed a fusion protein with β-galactosidase.

口蹄疫病毒(FMDv)VP1蛋白质141~160位氨基酸的肽段,是免疫活性肽,含20个氨基酸,用DNA合成仪合成这段免疫活性肽基因片段,共72bp,5'端带有Eco RI切点,3'端带有BamHI切点,利用Eco RI和BamHI切点将兔疫活性肽基因片段克隆到带肴1ac启动子的基因表达载体pWR590质粒中,以O型FMDV抗体做为特异的IgG,检查FMDV抗原活性肽基因片段的表达,从210个菌落中选到有明显表达的菌株8株。

he gene of human granulocyte-macrophage colony stimulating factor under the controlof CMV promoter was cloned into retroviral vector N2A to construct the expressing vectorN2A/CMV/hGM-CSF.This recombinant polasmid was transfected into packaging cell line bylipofectin and colonies capable of secreting recombinant retrovirus and GM-CSF were selectedby G418.The integration of GM-CSF gene was detected by PCR and Southerm blothybridization.The titer of recombinant retrovirus is about 10 ̄4CFU/ml.The supernatant of...

he gene of human granulocyte-macrophage colony stimulating factor under the controlof CMV promoter was cloned into retroviral vector N2A to construct the expressing vectorN2A/CMV/hGM-CSF.This recombinant polasmid was transfected into packaging cell line bylipofectin and colonies capable of secreting recombinant retrovirus and GM-CSF were selectedby G418.The integration of GM-CSF gene was detected by PCR and Southerm blothybridization.The titer of recombinant retrovirus is about 10 ̄4CFU/ml.The supernatant of thecolonies can support the growth of TF-1 cells.

应用基因工程的方法,将含有巨细胞病毒(CMV)启动子的基因片段和人粒细胞-巨噬细胞集落刺激因子(hGM-CSF)的cDNA,克隆进逆转录病毒载体N2A,得到重组质粒N2A/CMV/hGM-CSF.经脂质体包装并转染包装细胞,通过G418药物筛选,得到抗性克隆。经PCR和Southemblot检测证实,GM-CSF基因已整合到该克隆细胞的染色体上,获得的逆转录病毒滴度达10 ̄4CFU/ml,克隆细胞培养上清用TF-1细胞可检测到GM-CSF活性。

A pair of antibody VH primers containing EcoRI-start codon and stop codon Sall were used in PCR to amplify lAl2VHfrom recombinant plasmid plA12VH. The EcoRI-start-codon and stop-codon-Sall were added to the 5' and 3' of 1A12VH in plA12VH plasmid by PCR. 1A12VH was cloned into pSL301 plasmid and then cloned into pAcEEUL8 plasmid. BamHI sites were added to downstream of EcoRI and upstream of Sall within 1A12VH BamHI-lA12VH-BamHI could be subcloned into the unique BamHI restriction site of baculovirus expression...

A pair of antibody VH primers containing EcoRI-start codon and stop codon Sall were used in PCR to amplify lAl2VHfrom recombinant plasmid plA12VH. The EcoRI-start-codon and stop-codon-Sall were added to the 5' and 3' of 1A12VH in plA12VH plasmid by PCR. 1A12VH was cloned into pSL301 plasmid and then cloned into pAcEEUL8 plasmid. BamHI sites were added to downstream of EcoRI and upstream of Sall within 1A12VH BamHI-lA12VH-BamHI could be subcloned into the unique BamHI restriction site of baculovirus expression vector pAcCL29 for producing recombinant transfer vector 1A12VH-pAcCL29 (pAclA12Vn). Recombinant transfer vector pAclAl2VH in which the orientation of cloning gene is identical with that of baculovirus polyhedrosis gene was selected by digesting with a group of restriction enzymes. Sf9 cells were coinfected with 1A12VH gene and AcNPV genome. Recombinant viruses were screened by plaque assay and PCR.

设计一对含EcoRⅠ—起始码和终止码—SalⅠ的鼠抗体V_H引物,用PCR法对重组质粒p1A12V_H中插入基因1A12V_H进行突变,突变的PCR产物的序列测定表明,在1A12V_H基因两端成功地插入了EcoRⅠ—起始码和终止码—SalⅠ。将突变后1A12V_H依次克隆入pSL301质粒和pAcEEUL8质粒中,使ⅠA12V_H基因两端均接上BamHⅠ接头,用BanHⅠ酶切,将1A12V_H基因克隆入杆状病毒表达载体pAcCL29质粒的多角体基因中的唯一BamHⅠ切点处,经计算机分析,用BamHI;EcoRV/EcoRⅠ;SalⅠ三组酶切,筛选出克隆的1A12V_H基因方向与多角体启动子基因方向一致的重组杆状病毒转基因质粒pAc1A12V_H。CsCL—EB梯度平衡离心法纯化pAc1A12V_H,转化AcNPV基因组DNA,然后转染的sf9细胞,空斑筛选和纯化1A12V_H—AcNPV重组杆状病毒,并用PCR法作进一步鉴定。

 
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