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理论序列
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  theoretical sequence
     Results and conclusion: The results showed that the N-terminal amino acids sequence of recombinant α-galactosidase were identical with theoretical sequence completely;
     结果与结论:基因重组α半乳糖苷酶的N端序列与理论序列完全一致;
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     It is a continually evolving theoretical sequence about evolution phenomenon in universe from Lamarck's evolutionism,Darwinism, ID theory to life information evolutionism.
     从拉马克进化论、达尔文主义、ID论,到生命信息进化论,是一个不断“进化着”的关于宇宙中进化现象的理论序列
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  “理论序列”译为未确定词的双语例句
     The results showed the periodic variation with about 400 kyr during past 2.4 Myr. The amount of cross correlation coefficient between the filtered susceptibility data and the filtered earth's orbital eccentricity data attained at 0.93. The fact could be explained as the very slow modulation of the Earth's climate by the var-iation of earth's orbital geometry.
     数字分析结果表明,240万年以来地球的气候系统存在大约40万年的长周期变化,并且与地球轨道偏心率的理论序列在这一波段的变化存在显著的相关性,归一代序列的互相关系数达到93%。
短句来源
     The MALDI_TOF_MS was applied to measure the molecular weight of scFv. The experiment result was in conformity with the theoretical volume. The peptide mass mapping confirmed that the primary structure of scFv was correct and could determine the position of disulfide bond.
     基质辅助激光解析飞行时间质谱 (matrixassistedlaserdesorptionionization ,MALDI_TOF_MS)的结果证明 ,重组的scFv分子量与理论值相符 ,肽质量图谱的结果证明重组单链抗体的一级结构与理论序列是完全相符的 ,并确定了scFv蛋白中二硫键的位置
短句来源
     Results The expressed product reached a purity of more than 98% after purification,and its relative molecular weight was proved as 30 000 by SDS-PAGE and 30 780.522 by MOLDI-TOF-MS. The sequence of 15 amino acids at N-terminus of the expressed product was identical to that in theory.
     结果目的蛋白以包涵体形式存在,经变性、复性及柱层析纯化,纯度大于98%,SDS-PAGE测定相对分子质量为30000,MOLDI-TOF-MS测定其相对分子质量为30780·522,N-末端15个氨基酸与理论序列一致。
短句来源
  相似匹配句对
     p sequences or R.
     p序列和R.
短句来源
     SEQUENCE OPERATION THEORY AND ITS APPLICATIONS
     序列运算理论及其应用
短句来源
     Theory of regular sequences of noetherin modules over rings
     Noether模上的正则序列理论
短句来源
     n-FOLD NUMBER THEORY
     n重数理论
短句来源
     theory and generalization;
     理论总结;
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  theoretical sequence
Theoretical sequence of s-and t-incommensurate phases in quartz
      
Figure 1 shows the theoretical sequence derived from the integration of both paradigms.
      
If the gene sequence for the protein under study is known, experimental data can be compared to the theoretical sequence.
      
So again, from the information now at hand, one can assemble yet another plausible theoretical sequence of events that culminate in lithiasis.
      
This highly theoretical sequence in analysis is reserved for the most able students.
      
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Data analysis of susceptibility data of Luochuan section, which is used as one of the good proxy data of the past climatic variations, has been performed. The results showed the periodic variation with about 400 kyr during past 2.4 Myr.The amount of cross correlation coefficient between the filtered susceptibility data and the filtered earth's orbital eccentricity data attained at 0.93. The fact could be explained as the very slow modulation of the Earth's climate by the var-iation of earth's orbital geometry....

Data analysis of susceptibility data of Luochuan section, which is used as one of the good proxy data of the past climatic variations, has been performed. The results showed the periodic variation with about 400 kyr during past 2.4 Myr.The amount of cross correlation coefficient between the filtered susceptibility data and the filtered earth's orbital eccentricity data attained at 0.93. The fact could be explained as the very slow modulation of the Earth's climate by the var-iation of earth's orbital geometry. The prominent periodic fluctuations at about 100 kyr, which has been found in most geological records, is more complicated.No significant relation cxists between the climate system and the earth's orbital variations at 100 kyr band.

中国陕西洛川黄土─—古土壤序列的磁化率值可以作为气候长期变化的一种代用指标。数字分析结果表明,240万年以来地球的气候系统存在大约40万年的长周期变化,并且与地球轨道偏心率的理论序列在这一波段的变化存在显著的相关性,归一代序列的互相关系数达到93%。这一事实可以用地球轨道要素的变化缓慢地调制了地球的气候进行解释。然而,与冰河期有关的10万年时间尺度的变化是一个复杂的过程,在磁化率数据与偏心率数据之间没有显著的相关性。

The purification of a human anti_HBsAg single chain Fv(scFv) expressed by E. Coli was researched and the bioactivity and structure of the scFv was indentified.The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration. After purification, the purity of the scFv reached 97%. The results of direct ELISA and competitive ELISA proved that the refolding...

The purification of a human anti_HBsAg single chain Fv(scFv) expressed by E. Coli was researched and the bioactivity and structure of the scFv was indentified.The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration. After purification, the purity of the scFv reached 97%. The results of direct ELISA and competitive ELISA proved that the refolding scFv could be bound to HBsAg. Furthermore, it could inhibit the murine anti_HBsAg monclonal antibody to bind to HBsAg and the inhibit rate reached 40.3%. The MALDI_TOF_MS was applied to measure the molecular weight of scFv. The experiment result was in conformity with the theoretical volume. The peptide mass mapping confirmed that the primary structure of scFv was correct and could determine the position of disulfide bond.

对大肠杆菌表达的人源性抗乙型肝炎表面抗原 (HBsAg)单链抗体 (scFv)进行了纯化研究 ,并对单链抗体活性及其结构进行了鉴定 .大肠杆菌表达的单链抗体包涵体 ,用8mol/L尿素裂解 ,经过Ni离子螯合亲和层析和凝胶过滤两步纯化后 ,纯度 (w)达到97%以上 ,再通过透析复性除去尿素 .经间接ELISA和竞争性ELISA证明 ,复性后的scFv具有与HBsAg结合的活性 ,而且对鼠源抗HBsAg单克隆抗体的抑制率可达 4 0 .3% .基质辅助激光解析飞行时间质谱 (matrixassistedlaserdesorptionionization ,MALDI_TOF_MS)的结果证明 ,重组的scFv分子量与理论值相符 ,肽质量图谱的结果证明重组单链抗体的一级结构与理论序列是完全相符的 ,并确定了scFv蛋白中二硫键的位置

[Abstract] Objective To study on the molecular structure and biological activity of recombinant human IFN-7. Methods The biological activity of IFN-7 manufactured by Shanghai Institute of Biological Products (SIBP) was detected by cytopathy inhibition and calculated into international unit using Japanese IFN-y reference as standard, and the relative molecular weight of it was detected by 4 methods. Results The specific activity of the IFN-γmanufactured by SIBP was not less than 3.0 ×107 IU/mg protein,and the...

[Abstract] Objective To study on the molecular structure and biological activity of recombinant human IFN-7. Methods The biological activity of IFN-7 manufactured by Shanghai Institute of Biological Products (SIBP) was detected by cytopathy inhibition and calculated into international unit using Japanese IFN-y reference as standard, and the relative molecular weight of it was detected by 4 methods. Results The specific activity of the IFN-γmanufactured by SIBP was not less than 3.0 ×107 IU/mg protein,and the relative molecular weight of it was lower than 16900. It consisted of only 129 amino acids,and the sequences of 15 to 19 amino acids at the N - teminal of it were in accord with those in theory. Conclusion The deletion of amino acids was observed at the C - tenninal of IFN-y manufactured by SEBP. However, the biological activity of the EFN-γhas not been influenced.

目的 了解重组人干扰素γ的分子结构及其生物活性。方法 采用细胞病变抑制法,以日本干扰素γ参考品为标准,计算国际单位。以4种不同方法检测相对分子质量。结果 比活性≥3.0×107 IU/mg蛋白,相对分子质量低于理论值16900。氨基酸分析结果只有129个氨基酸,少于应有数目。N端氨基酸序列分析结果,15-19个氨基酸与理论序列相符。结论 重组人干扰素γ C末端氨基酸有缺失,但不影响其生物活性。

 
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