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肿瘤学
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阴性区
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  negative region
     Background &Objective:It has been reported that apoptotic r ate of tumor infiltrating lymphocytes(TIL)in Fas ligand(FasL)positive region was higher than in FasL negative region,which indicated that tumor cells could kill tumor infiltrating lymphocytes through h igh-expressed FasL.
     背景与目的:研究发现,肿瘤组织中Fas配体阳性表达区肿瘤浸润淋巴细胞的凋亡率比FasL阴性区高,推测肿瘤细胞可通过增加表达FasL来杀伤肿瘤浸润淋巴细胞。
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     Apoptotic rate of tumor cells was higher in FasL positive region( n =25)(81 2%) than that in FasL negative region( n =25)(47 4%)( P <0 01).
     FasL表达阳性区(n =2 5 )肿瘤细胞的凋亡率 (81 2 % )比FasL阴性区 (n =2 5 ) (47 4 % )高 (P <0 0 1)。
短句来源
     ⑤ Apoptotic r ate of tumor cells was higher in FasL negative region (81.2%) than that in FasL positive region (47.4%, P <0.01).
     ⑤FasL表达阴性区肿瘤细胞的凋亡率 (81.2 % )比FasL阳性区 (3 7.4% )高 (P <0 .0 1)。
短句来源
     In the same tumor tissue,FasL expression was not even. Apoptosis rate of tumor cells was higher in FasL positive region(7.09±0.18%) than that in FasL negative region(4.75±0.64%)(P< 0.05).
     (4)在同一结肠腺癌组织芯片中,FasL表达不均匀:FasL表达阳性区肿瘤细胞的凋亡率(7.09±0.18%)比FasL阴性区(4.75±0.64%)高(P<0.05)。
短句来源
     ③In the same tumor tissue,FasL expression was not even. Apoptosis rate of tumor cells was higher in FasL positive region(n=45)(5.013%) than that in FasL negative region(n=5)(2.815%)(P<0.05).
     ③在同一结直肠腺癌组织切片中,FasL表达不均匀:FasL表达阳性区(n=45)结直肠癌细胞的凋亡率(5.013%))比FasL阴性区(n=5)(2.815%)高(P<0.05)。
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  “阴性区”译为未确定词的双语例句
     METHODS\ Formalin fixed, paraffin embedded sections of 28 HCC, Para_HCC with strongly HBsAg(+) area (P 2) and HBsAg(-) area (P 1) in one low_power size tested by microdissection PCR of S and C genes.
     方法福尔马林固定,石蜡包埋肝癌及癌旁组织28例,HBsAg免疫组化后的切片分别切割癌组织、癌旁HBsAg强阳性区(P2)及癌旁HBsAg阴性区(P1)各1个低倍视野,作S及C基因PCR。
短句来源
     CONCLUSIONS\ The integrated HBVDNA is imcomplete in HCC tissue. The positivity rates of S gene in HBsAg strongly positive area and negative area were similar.
     结论癌组织中整合的HBVDNA为残缺不全的,HBsAg强阳性与阴性区的S基因检出无显著性差异。
短句来源
     The distributions of p53 protein and apoptotic cells observed through double staining showed a tendency to decrease of apoptosis in p53 protein positive areas, while showing a tendency to increase in p53 negative areas.
     双染原位观察p53蛋白与凋亡细胞的分布发现:p53阳性区凋亡细胞有明显减少趋势,而p53阴性区凋亡细胞分布则有增加趋势。
短句来源
     Immunohistochemistry and terminal deoxynucleotidyl tra nsferase mediated dUTP nick end labeling assay (TUNEL) were used to detect the cell count of TIL and the count of TIL being apoptosis in both negative and pos i tive regions of FasL in the consecutive tissue slices of cancers respectively.
     应用免疫组织化学染色和原位末端杂交标记法 (TUNEL)检测 3 0例大肠癌组织连续切片的FasL表达阴性区和阳性区中TIL细胞总量和正处于凋亡状态的TIL细胞数。
短句来源
     ④ The cell count of TIL being apoptotic in the positive regions was as 2.13 times as that in the negative ones.
     ④同一组织切片中 ,FasL阳性表达区正在凋亡的TIL细胞数比FasL阴性区增多 ,前者是后者的 2 .13倍。
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  相似匹配句对
     Culture Region
     文化
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     and f) synclinorium.
     f)复向斜
短句来源
     PAS was negative.
     PAS阴性
短句来源
     Diagnostic value of CT on negative calculus in third ureter stenosis area
     输尿管第三狭窄阴性结石的CT诊断价值
短句来源
     120 sera from normal population in epidemic area and 50 sera from non - epidemic area showed negative results.
     120人份流行健康人血清全为阴性;
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  negative region
The continuous transition is traced of the line of achievable superheats of investigated liquids from the positive to negative region of pressures.
      
The formal potential E0p only slightly depends on the concentration of HNO3 and is shifted toward the negative region by ≈1.0 V as compared with the standard values.
      
The potential E0p is independent of the concentrations of HNO3 and KPW and is shifted toward the negative region by 70 mV (ΔE0p) as compared to 1 M HClO4.
      
Within this negative region a ten base-pair sequence was detected that shows high homology to a sequence located within a negative control region of the CYC1 gene and some homology to the negative control elements of the S.
      
The profile of sodium-dependent calcium uptake versusEK suggested that calcium influx through electrogenic sodium/calcium exchange may be much more affected by the positive region of the cardiac action potential than by the negative region.
      
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AIMS\ To study S and C genes of HBVDNA in hepatocellular carcinoma(HCC) and para_HCC tissue by microdissection (location) PCR. METHODS\ Formalin fixed, paraffin embedded sections of 28 HCC, Para_HCC with strongly HBsAg(+) area (P 2) and HBsAg(-) area (P 1) in one low_power size tested by microdissection PCR of S and C genes. RESULTS\ Positivity rate of S gene was higher than that of C gene in HCC(P<0.05), while in para_HCC there were no significant difference between them. In para_HCC the positivity rates...

AIMS\ To study S and C genes of HBVDNA in hepatocellular carcinoma(HCC) and para_HCC tissue by microdissection (location) PCR. METHODS\ Formalin fixed, paraffin embedded sections of 28 HCC, Para_HCC with strongly HBsAg(+) area (P 2) and HBsAg(-) area (P 1) in one low_power size tested by microdissection PCR of S and C genes. RESULTS\ Positivity rate of S gene was higher than that of C gene in HCC(P<0.05), while in para_HCC there were no significant difference between them. In para_HCC the positivity rates of S and C genes in P 1 and P 2 were similar. The positivity rates of S and/or C genes in highly differentiated HCC were lower than that of middle differentiated HCC (P<0.05). CONCLUSIONS\ The integrated HBVDNA is imcomplete in HCC tissue. The positivity rates of S gene in HBsAg strongly positive area and negative area were similar.

目的采用定点PCR技术,研究HBV的S和C基因在肝癌与癌旁组织的存在状态,探讨癌旁HBsAg表达与S基因的关系。方法福尔马林固定,石蜡包埋肝癌及癌旁组织28例,HBsAg免疫组化后的切片分别切割癌组织、癌旁HBsAg强阳性区(P2)及癌旁HBsAg阴性区(P1)各1个低倍视野,作S及C基因PCR。结果癌组织S基因较C基因检出率高(18/28vs10/28,P<0.05),癌旁二者无差异;癌旁中P1与P2的S及C基因检出率无显著性差异;高分化肝癌S和/或C基因检出率较中分化者低(2/5vs20/28,P<0.05)。结论癌组织中整合的HBVDNA为残缺不全的,HBsAg强阳性与阴性区的S基因检出无显著性差异。

Purpose: To investigate the effects of apoptosis and its regulating genes in malignant transformation of breast cancer and its precancerous lesions Methods: The expression of apoptosis and the positive expression of p53, bcl-2 proteins were observed in 31 cases of breast cancer in situs, 20 breast dysplasia and 20 breast hyperplasia by using DNA nick end labeling (TUNEL) technique and immunohistochemical staining Eight normal breast tissues were used as control Results: The apoptotic indexes in breast...

Purpose: To investigate the effects of apoptosis and its regulating genes in malignant transformation of breast cancer and its precancerous lesions Methods: The expression of apoptosis and the positive expression of p53, bcl-2 proteins were observed in 31 cases of breast cancer in situs, 20 breast dysplasia and 20 breast hyperplasia by using DNA nick end labeling (TUNEL) technique and immunohistochemical staining Eight normal breast tissues were used as control Results: The apoptotic indexes in breast dysplasia and breast hyperplasia were significantly higher than those in breast cancer and normal ( P< 0 01) The apoptotic index in breast cancer was higher than those in normals ( P< 0 05) The positive expression rate of p53 protein in breast cancer and dysplasia were higher than that in normal control ( P< 0 05) The majority of apoptotic cells were located in the negative expression area of p53, bcl-2 proteins, a few of apoptotic cells were located in the positive area The apoptotic index in negative group of p53, bcl-2 proteins was higher than that in positive group ( P< 0 05) Conclusion: The abnormal regulation of apoptosis may play an important role in malignant transformation of breast cancer,and p53, bcl-2 proteins may inhibit apoptosis

目的 :通过观察乳腺癌及其癌前病变中细胞凋亡和凋亡调控基因 p5 3、bcl- 2的表达 ,探讨细胞凋亡与凋亡调控基因在乳腺组织恶性转化进程中的作用。方法 :利用DNA缺口末端标记技术和免疫组织化学染色 ,原位观察 31例乳腺癌 ,2 0例乳腺不典型增生和 2 0例乳腺单纯性增生中细胞凋亡和p5 3、bcl- 2蛋白的表达 ,以 8例正常乳腺组织作为对照。结果 :乳腺不典型增生和单纯性增生中细胞凋亡指数显著高于乳腺癌及正常乳腺组织 (P <0 0 1) ,乳腺癌中细胞凋亡指数高于正常乳腺组织 (P <0 0 5 )。乳腺癌和乳腺不典型增生中 p5 3蛋白阳性率分别高于正常乳腺组织 (P <0 0 5 )。凋亡细胞多分布在 p5 3、bcl- 2蛋白阴性区 ,阳性区仅有少量分布 ,且 p5 3、bcl- 2蛋白阴性组细胞凋亡指数高于阳性组 (P <0 0 5 )。结论 :细胞凋亡调控失调在乳腺组织恶性转化进程中起重要的作用。突变型p5 3、bcl- 2蛋白可抑制细胞凋亡。

To investigate the relationship between p53 and apoptosis in different stages of malignant transformation of large intestine epithelia. Methods:Using DNA nick end labeling technique for apoptotic cells, immunohistochemical staining for p53 protein and double staining for both,the density and distribution of the apoptotic cells and positive expression of p53 protein were observed in situ in 21 cases of malignantly transformed adenomas and 27 cases of adenocarcinomas of the large intestine. Fifteen cases of non-tumor...

To investigate the relationship between p53 and apoptosis in different stages of malignant transformation of large intestine epithelia. Methods:Using DNA nick end labeling technique for apoptotic cells, immunohistochemical staining for p53 protein and double staining for both,the density and distribution of the apoptotic cells and positive expression of p53 protein were observed in situ in 21 cases of malignantly transformed adenomas and 27 cases of adenocarcinomas of the large intestine. Fifteen cases of non-tumor mucosas were used as controls. Results:The density of the apoptotic cells in benign areas of adenomas was significantly higher than that in non-tumor mucosas,malignant areas of adenomas or adenocarcinomas respectively(all P < 0.01). The densities of p53 protein positive cells in malignant areas of adenomas and adenocarcinomas were significantly higher than those in benign areas of adenomas and non-tumor mucosas respectively (all P< 0.01). The distributions of p53 protein and apoptotic cells observed through double staining showed a tendency to decrease of apoptosis in p53 protein positive areas, while showing a tendency to increase in p53 negative areas. Conclusion: Probably through decreasing in apoptosis,p53 mutations play a critical part in the formation and progression of carcinomas of large intestine.

目的:观察大肠腺癌,腺瘤恶变区及非恶变区细胞凋亡及其调控基因p53的表达状态,探讨它们在大肠上皮恶性转化进程中的作用及二者的关系。方法:利用DNA缺口末端标记技术,p53蛋白免疫组化染色及双重染色技术,原位观察27例大肠腺癌及21例腺瘤恶变标本中凋亡细胞和p53阳性表达细胞的密度与分布,以8例非肿瘤大肠粘膜作为对照。结果:腺瘤非恶变区凋亡细胞密度分别高于腺癌(P<0.01)、腺瘤恶变区(P<0.01)及非肿瘤粘膜(P<0.01)。腺癌及腺瘤恶变区p53蛋白阳性细胞密度均高于腺瘤非恶变区(P<0.01)及非肿瘤粘膜(P<0.01)。双染原位观察p53蛋白与凋亡细胞的分布发现:p53阳性区凋亡细胞有明显减少趋势,而p53阴性区凋亡细胞分布则有增加趋势。结论:p53蛋白突变在大肠癌发展中具重要的促恶变形成作用,其作用可能是通过抑制细胞凋亡而实现的。

 
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