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细胞质游离钙
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  cytosolic free calcium
     Determination of Cytosolic Free Calcium Concentration in Toxoplasma Gondii Tachyzoite
     弓形虫速殖子细胞质游离钙浓度的测定
短句来源
  cytoplasmic free calcium
     Effect of phospholipase C on human platelet cytoplasmic free calcium concentration
     磷酯酶C对人血小板细胞质游离钙离子浓度的影响
短句来源
     Determination of the Concentration of Cytoplasmic Free Calcium Following Econazole-induced Apoptosis of Leukemia Cells WEHI-3 by Fura-2/AM
     用Fura-2/AM测定益康唑诱导鼠白血病WEHI-3细胞凋亡后细胞质游离钙浓度
短句来源
     \ Methods\ Toxoplasma gondii tachyzoites of the RH strain collected from the peritoneal fluid in mice 3 days after infection,using a fluorescence dye(Fura\|2) to bind cytoplasmic free Ca\+\{2+\}. \ Cytoplasmic free calcium concentration([Ca\+\{2+\}]i) in toxoplasma gondii tachyzoite was determined by spectrofluorophotometer and Super Ion Probe Software computer software.
     方法 以急性期感染小鼠腹水收集的RH株刚地弓形虫速殖子为材料 ,采用荧光染料 Fura- 2与细胞质游离钙结合 ,通过荧光分光光度计技术和 SuperIon Probe Software计算机软件测定弓形虫速殖子细胞质游离钙浓度 ([Ca2 + ]i)。
短句来源
     The experimental conditions to detect human platelet cytoplasmic free calcium were studied in human and rat by Fura-2/AM fluorescence technique.
     对荧光法测定磷酯酶C对血小板细胞质游离钙离子浓度作用的实验条件进行了研究. 结果表明,在本实验条件下,Fura 2/AM合适的浓度为4μmol/L;
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  “细胞质游离钙”译为未确定词的双语例句
     Measurement of Cytoplasmic Calcium Fluorescence in Arabidopsis thaliana Pollen Cells
     拟南芥花粉细胞质游离钙离子荧光测定法
短句来源
     The influence of antibodies against the second extracellular loop of the AT_1 receptor on intracellular free Ca~(2+) level of VSMC
     血管紧张素Ⅱ1型受体胞外第二环肽抗体对大鼠血管平滑肌细胞质游离钙水平的影响
短句来源
     In germinating pollens,the cytoplasmic calcium concentration was 246±38nmol/L which near to measuring result with other methods,it futher showed this method is reliable through examining of cytoplasmic calcium in Arabidopsis thaliana pollen cells using Fura-2/AM.
     花粉粒细胞萌发状态下的[Ca2+]i=246±38nmol/L,该值与花粉粒细胞萌发状态下游离钙离子浓度用其它方法测得值接近,进一步表明所建立的用Fura-2/AM检测拟南芥花粉粒细胞质游离钙离子的方法是可靠的。
短句来源
     Objective To explore the effect of antibodies against the second extracellular loop of the angiotonin Ⅱ receptor type 1(AT_1 receptor) on the intracellular free Ca~(2+) level in rat vascular smooth muscle cells(VSMC).
     目的观察血管紧张素Ⅱ1型受体(AT1受体)细胞外第二环肽抗体对培养大鼠主动脉血管平滑肌细胞(VSMC)细胞质游离钙水平的影响。
短句来源
     We also measure the free Ca~(2+) level in germinating pollen grains, and found that exogenous purified calmodulin was able to increase the free Ca~(2+) level, and their effects on Ca~(2+) level were inhibited by U-73122.Two kinds of full length of PLC cDNAs (LdPLCl and LdPLC2) were cloned from Lily pollen.
     利用confocal技术检测了PLC对花粉细胞游离钙离子的影响,观察到U-73122抑制细胞质游离钙离子水平的升高和钙震荡。 上述结果表明,在花粉体系中,PLC-IP_3-Ca~(2+)做为G蛋白的下游组分,参与了G蛋白介导的胞外CaM的信号传递途径。
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  相似匹配句对
     Determination of Cytosolic Free Calcium Concentration in Toxoplasma Gondii Tachyzoite
     弓形虫速殖子细胞质游离浓度的测定
短句来源
     Measurement of Cytoplasmic Calcium Fluorescence in Arabidopsis thaliana Pollen Cells
     拟南芥花粉细胞质游离离子荧光测定法
短句来源
     Effect of phospholipase C on human platelet cytoplasmic free calcium concentration
     磷酯酶C对人血小板细胞质游离离子浓度的影响
短句来源
     Cytoplasmic free Ca2+ concentration(\i) in PASMCs was investigated by fluorophotometry.
     荧光光度法检测细胞质游离离子浓度([Ca2+]i);
短句来源
     ANTIHYPERTENSIVE DRUGS AND PLATELETS IONIZED CALCIUM
     抗高血压药与血小板游离
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  cytosolic free calcium
Role of increased cytosolic free calcium concentration in myocardial ischemic injury
      
Increases in cytosolic free calcium concentration ([Ca2+]I) may play an important role in myocardial ischemic injury.
      
Hypoxia increases cytosolic free calcium in porcine pulmonary arterial endothelial cells
      
Alteration of cytosolic free calcium ([Ca2+1]) in hypoxia was studied with fluorescent probe, Fura-2 in cultured porcine pulmonary arterial endothelial cells.
      
Effects of ATP on cytosolic free calcium ([Ca2+]i) in single porcine pulmonary artery endothelial cell were studied.
      
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  cytoplasmic free calcium
Effect of interleukin-1β on the elevation of cytoplasmic free calcium of the cultured hippocampal neurons induced by L-glutamate
      
Oscillations in cytoplasmic free calcium concentration in human pancreatic islets from subjects with normal and impaired glucose
      
This strongly suggests that there is no correlation between maximal stimulation of cytoplasmic free calcium, and maximal stimulation of migration.
      
The mobilization of cytoplasmic free calcium is also normal.
      
Cytoplasmic free calcium has been observed in fura 2-loaded stria vascularis of the guinea pig.
      
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Calcium distribution and mobilization during mechanical stimulation in outer hair cells of the guinea pig were monitored using laser scanning confocal microscopy and co-loaded fluo-3 and fu-ra-red fluorescent probes. Spatial calcium gradients were revealed among various subcellular areas. The ratios of the fluorescence intensity of fluo-3 and fura-red were 1. 71±0. 85, 1. 61±0. 75, 1. 47±0. 65 and 1. 39±0. 66 for the cytoplasm, the cytoplasmic membrane, the cuticular plate and the nucleus respectively, indicating...

Calcium distribution and mobilization during mechanical stimulation in outer hair cells of the guinea pig were monitored using laser scanning confocal microscopy and co-loaded fluo-3 and fu-ra-red fluorescent probes. Spatial calcium gradients were revealed among various subcellular areas. The ratios of the fluorescence intensity of fluo-3 and fura-red were 1. 71±0. 85, 1. 61±0. 75, 1. 47±0. 65 and 1. 39±0. 66 for the cytoplasm, the cytoplasmic membrane, the cuticular plate and the nucleus respectively, indicating

豚鼠耳蜗分离外毛细胞经fluo-3和fura-red染色后,用激光扫描共聚焦显微镜监测其胞内游离钙的空间分布和动态变化,以fluo-3/fura-red荧光比值大小指示游离钙浓度的高低。外毛细胞胞内游离钙呈不均匀分布,荧光比值分别为细胞质1.71±0.85,质膜1.61±0.75,表皮板1.47±0.65及胞核1.39±0.66,显示细胞质游离钙浓度最高而胞核游离钙浓度最低。静息状态下连续扫描时荧光比值变化小于0.1,而在受到机械刺激后荧光比值增加幅度达0.3—1.2。实验结果表明,fluo-3和fura-red双发射比例法能更准确反映听毛细胞胞内钙的空间分布和动态变化,第二信使钙可能参与了听毛细胞的机械-电换能过程,研究了换能过程中听毛细胞钙信号的时空模式。

Objective\ To further study the mechanism of Ca\+\{2+\} channels in interaction between both toxoplasma and host cell.\ Methods\ Toxoplasma gondii tachyzoites of the RH strain collected from the peritoneal fluid in mice 3 days after infection,using a fluorescence dye(Fura\|2) to bind cytoplasmic free Ca\+\{2+\}.\ Cytoplasmic free calcium concentration([Ca\+\{2+\}]i) in toxoplasma gondii tachyzoite was determined by spectrofluorophotometer and Super Ion Probe Software computer software.\ Results\ The cytoplasmic...

Objective\ To further study the mechanism of Ca\+\{2+\} channels in interaction between both toxoplasma and host cell.\ Methods\ Toxoplasma gondii tachyzoites of the RH strain collected from the peritoneal fluid in mice 3 days after infection,using a fluorescence dye(Fura\|2) to bind cytoplasmic free Ca\+\{2+\}.\ Cytoplasmic free calcium concentration([Ca\+\{2+\}]i) in toxoplasma gondii tachyzoite was determined by spectrofluorophotometer and Super Ion Probe Software computer software.\ Results\ The cytoplasmic free[Ca\+\{2+\}]i in nomal toxoplasma gondii tachyzoite were(205.02±18.6)nmol/L, concentration which are in the range observed in many studies with resting eukaryotic cells.\ Conclusion\ This method for measurement of Ca\+\{2+\} is simple, applicable and less expensive.\;

目的 研究弓形虫与宿主细胞之间相互作用的钙通道机制。 方法 以急性期感染小鼠腹水收集的RH株刚地弓形虫速殖子为材料 ,采用荧光染料 Fura- 2与细胞质游离钙结合 ,通过荧光分光光度计技术和 SuperIon Probe Software计算机软件测定弓形虫速殖子细胞质游离钙浓度 ([Ca2 + ]i)。 结果 正常弓形虫速殖子[Ca2 + ]i为 (2 0 5 .0 2± 18.6 ) nm ol/ L,符合文献报道的静息状态下真核细胞 [Ca2 + ]i浓度范围。 结论 本方法简便、易行 ,相对费用低 ,适用于国内实验室

Objective To investigate the changes and roles of cytosolic free calcium concentration (i) in hematoporphyrin monomethyl ether(HMME) photodynamic therapy(HMME PDT) treated HeLa cells Methods The percentages of HeLa cells death and i were respectively measured by MTT methods and fluorospectrophotometry post PDT treatment Simultaneously, Sarco/endoplasmic reticulum calcium ATPase2 (SERCA2) protein degradation in HMME PDT treated HeLa cells was analyzed by Western blotting Results HMME PDT immediately...

Objective To investigate the changes and roles of cytosolic free calcium concentration (i) in hematoporphyrin monomethyl ether(HMME) photodynamic therapy(HMME PDT) treated HeLa cells Methods The percentages of HeLa cells death and i were respectively measured by MTT methods and fluorospectrophotometry post PDT treatment Simultaneously, Sarco/endoplasmic reticulum calcium ATPase2 (SERCA2) protein degradation in HMME PDT treated HeLa cells was analyzed by Western blotting Results HMME PDT immediately induced i increase in a PDT dose dependent manner, i decreased by BAPTA/AM rescued HeLa cells from death HMME PDT had also caused SERCA2 protein degradation in a PDT dose dependent manner Conclusions i can immediately increase in HeLa cells post HMME PDT i increase, which may correlated to SERCA2 protein degradation caused by HMME PDT, can promote HeLa cell death

目的了解钙离子在血卟啉单甲醚-光动力学疗法(HMME-PDT)处理后HeLa细胞中的变化和作用。方法Fura-2/AM孵育荧光分光光度计检测细胞质游离钙浓度犤Ca2+犦i的变化,MTT法分析PDT及钙离子对HeLa细胞存活率的影响,Western印迹法分析HMME-PDT后HeLa细胞的肌浆/内质网钙泵(SERCA2)蛋白的降解情况。结果HMME-PDT处理后即刻HeLa细胞内犤Ca2+犦i就开始增高,且与PDT剂量成正相关用,BAPTA/AM拮抗犤Ca2+犦i的升高可增加HeLa细胞的存活率。同时HMME-PDT可引起SERCA2蛋白的降解。结论HMME-PDT处理后HeLa细胞内犤Ca2+犦i可迅速增高,犤Ca2+犦i升高可能与SERCA2蛋白的降解相关,并可促进HeLa细胞的死亡。

 
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