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  carbon source
    By the fermentation in shake flask,it was studied that the influence of temperature,the shaking speed of shake flask,the kind and concentration of carbon source and the kind and concentration of nitrogenous source on the production of dicarboxylic acid by fermentation of Candida tropicalis.
    通过摇瓶发酵,研究温度、摇瓶转速、碳源种类和浓度、氮源种类和浓度对热带假丝酵母(Candida tropicalis)产酸的影响.
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    The effect of many vitamins on the production of pyruvic acid by Torulopsis glabrata TK006 with glucose as carbon source and (NH4)2SO4 as sole nitrogen source was investigated.
    报道了几种维生素对光滑球拟酵母(Torulopsis glabrata)TK006以葡萄糖为碳源、硫酸铵为唯一氮源生产丙酮酸的影响。
短句来源
    The result showed that when sucrose as carbon source,peptone or peptone plus beef paste as nitrogen source,original pH 6.0,the GABA production of As-8 strain could reach higher yield(4.2g/L) and was genetic stable after growing up to 15 generations.
    菌株As-8转化富集GABA的适宜条件为:pH 6.0,以蔗糖为碳源,蛋白胨为唯一氮源或蛋白胨和牛肉膏为复合氮源。 产生的GABA含量最高可达4.2g/L,并能稳定遗传。
短句来源
    The results showed that the optimal carbon source was glucose,the optimal nitrogen source was peptone,the optimal nitrogen concentration was 2% and the optimal metallic ion was Mn2+ for Y9 SOD producing.
    该菌株产SOD的适宜碳源为葡萄糖,氮源为蛋白胨,氮源浓度为2%,适宜金属离子为Mn2+。
短句来源
    With pretreated rice straw powder as primary carbon source, optimal cultivation medium composition obtained by orthogonal test was as following (g/L ): rice straw powder, 33;
    并首次利用预处理的稻草粉为主要碳源,经过正交实验,得到了康氏木霉ZJ5发酵产纤维素酶的最佳培养基组成(g/L):稻草粉,33;
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  carbon sources
    STUDY ON GROWTH AND HYDROGEN PRODUCTION OF CLOSTRIDIUM BUTYRICUM LMG1213, A69 AND CLOSTRIDIUM PASTEURIANUM LMG3285~T IN DIFFERENT CARBON SOURCES
    丁酸梭菌LMG1213、A69及巴氏梭菌LMG3285~T在不同碳源上生长产氢的研究
短句来源
    Effects of Carbon Sources and Concentration on the Culture of Phaffia rhodozyma
    不同碳源及其浓度对红发夫酵母培养的影响
短句来源
    Fermentation conditions for the cellulase producing were studied in shaking flasks by Bacillus sp. AC-2, including inoculum age, inoculum volume, initial pH value, fermentation temperature, carbon sources, ratio of C/N, nitrogen sources and surface active agent, etc.
    本文选用嗜碱性芽孢杆菌Bacillus sp.AC-2发酵生产纤维素酶,在摇瓶条件下对Bacillus sp.AC-2的产酶工艺进行了研究,包括种子培养时间、接种量、初始pH值、培养温度、碳源、C/N、氮源、无机盐和表面活性剂等。
短句来源
    Fermentation culture in 6L fermentor also indicated that the expression of vgb gene in 4AK4 resulted in much more rapid cell growth and PHBHHx production. When 1:1 co-substrates were used as carbon sources, 38.4 g L-1 DCW containing 52 wt% PHBHHx with 7.4 mol% 3HHx fraction was obtained in 42 hours of fermentation culture in 6L fermentor;
    在6 L发酵罐中,当以1:1的月桂酸和葡萄糖酸钠为碳源时,42 h发酵罐培养能获得38.4 g L-1 DCW,PHBHHx含量及3HHx组成分别为52 wt%和7.4 mol%;
短句来源
    The influence on producing inulinase with different carbon sources, nitrogen sources , initial pH, metal chemical compound was studied, the results indicated that the optimum fermented medium was: inulin 2.5% ; yeast extract 0.8% ; (NH_4)_2HPO_4 0.2% ;
    研究了碳源、氮源、pH、培养温度以及添加不同的金属化合物等因素对产酶的影响得出优化产酶培养基为:菊粉2.5%,酵母膏0.8%,(NH_4)_2HPO_4 0.2%,MgSO_4·7H_2O 5mmol/L,pH 5.5。
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  carbon resource
    1. Screening of carbon resourceThe result showed that carbon resource A was the best for VB12 fermentation.
    1.碳源的筛选 实验结果表明碳源A是VB_(12)发酵的最佳碳源
短句来源
    To produce arachidonic acid (AA) and eicosapentaenoic acid (EPA) by organic wastewater of soybean products, the effects on the AA and EPA yield of culturing time, carbon resource concentration, nitrogen resource concentration and dilution times were studied.
    为探讨用豆制品废水生产花生四烯酸(AA)和二十碳五烯酸(EPA),研究了不同培养时间、碳源浓度、氮源浓度和黄泔水稀释倍数对轮梗霉产这两种脂肪酸的影响。
短句来源
    The effects of time, temperature, pH, carbon resource, nitrogen resource on the effect of xylanase by strain CY8 were studied.
    研究了培养时间、温度、pH、氮源、碳源对菌株产木聚糖酶的影响,最佳的碳源m(麦麸)/m(玉米秸杆)为3∶7,最佳的氮源为尿素。
短句来源
    After orthogonal experiment, the optimum fermentation conditions of the mutant were obtained as follows: concentration of the major carbon resource 4 %, ratio between bran and corncob 5:5, concentration of glucose 0.1%, concentration of ammonium oxalate as supplemental nitrogen resource 2.0%, the initial pH of liquid medium 5.0, 100mL/250mL flask.
    经过正交试验得出突变株产酶的最佳发酵条件为 :主碳源浓度 4%、麸皮与玉米芯的比例为 5∶5、葡萄糖浓度 0 1 %、草酸铵浓度 2 0 % ,培养基初始pH为 5 0 ,2 5 0mL三角瓶的装液量为 1 0 0mL。
短句来源
    (3) Through single factor experiment, the optimal carbon resource was sucrose and optimal nitrogen resource was yeast extraction powder for Rhodotorula glutinis producing carotenoid.
    (3)通过单因素实验发现发酵红酵母产类胡萝卜素的最佳碳源为蔗糖,最佳氮源为酵母粉。 添加番茄汁(3.0mL/L)或花生油(8mL/L)有利于生产。
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  c source
    The results showed that optimal solid culture medium was that the optimal C source was wheat straw∶wheat surface=4∶6,stuff∶water=1∶2.5,adding 1%(NH4)2SO4,0.1% Tween80,the optimal original pH is 5.0(for FPA) or 3.0(for CMC). The optimal culture temperature was 28 ℃,incubation time was 96 hours.
    结果表明:最佳碳源为小麦秸粉∶麦麸(4∶6),料∶水=1∶2.5,加入1%(NH4)2SO4,0.1%Tween80,初始pH值为5.0(滤纸酶活)或为3.0(CMC酶活),28℃下培养96 h时其产纤维素酶活力最高.
短句来源
    TG≡866 Was breeded from TG-3 as parent mutagenized by ultraviolet and DES. The mutant has characteristics of high glucose resistance, high glutamate, resistance, monofluorine acetate resistance, litter grouth with only glutamate as C source and good grouth with only sucinate as C source.
    以TG—3菌为亲株,经紫外线、硫酸二乙酯的复合诱变,定向选育抗单氟乙酸、耐高糖、耐高谷氨酸、以谷氨酸为唯一碳源不长和以琥珀酸为唯一碳源生长良好的突变株,选育出TG—866谷氨酸高产菌.
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  carbon source
To model the carbon cycle and predict the carbon source/sink of forest ecosystems, we must first understand the relative contributions of root/rhizosphere and microbial respiration to total soil respiration under elevated CO2 concentrations.
      
Glucose was the most suitable carbon source for LAB-producing EPS.
      
Two bacterial strains, Py1 and Py4, have been tamed and isolated through long cultivation with polycyclic aromatic hydrocarbon-pyrene as the single carbon source.
      
Besides precursors, methane as the carbon source and hydrogen as the ablation, oxygen or H2O was alternatively inlet into the reactive chamber at the pressure of 0.05 MPa.
      
Influence of carbon source and temperature on the denitrifying phosphorus removal process
      
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  carbon sources
Toxicity of Mercaptoethanol to Mutant Strains of the Yeast Pichia methanolica Growing on Different Carbon Sources
      
Consumption of Organic Carbon Sources and Biosynthesis of Lactic Acid by the Photosynthetic BacteriumRhodobactersp.
      
was carried out during solid-state and submerged fermentation using different carbon sources and crude enzymes were characterized.
      
The content of γ-linoleic acid was higher when carbohydrates (glucose and molasses) were used as carbon sources and urea was used as a nitrogen source.
      
Enzyme and respirometric assays were carried out with cells harvested from log phase and stationary phase from medium with different carbon sources and nitrogen levels.
      
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  carbon resource
The results showed that when acetate was used as the sole carbon resource in the influent, the sludge acclimatized under anaerobic/aerobic operation had good phosphorus removal ability.
      
It is feasible to produce succinic acid using lactose from cheese whey as carbon resource by A.
      
The potential importance of faecal output and bacterial production as a carbon resource for benthic communities near mussel culture areas is discussed.
      
The observed TNC allocation patterns could not adequately explain the variation among species in the production of new growth and it was concluded that some factor(s) other than the carbon resource was limiting regrowth.
      
Diatoms represented only a supplementary carbon resource available for higher trophic levels, whereas, until now, they were considered as the principal food of oysters in coastal ponds.
      
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  c source
One hour after the start of supplying the solutions, an assimilation clip chamber was fastened to the middle part of the shoot (14C source area), and 14CO2 was blown through in the light for 2.5 min.
      
In plants supplied with water or urea, 17-20% of labeled carbon was found below the 14C source area of the shoot, in nitrate type of treatment, only 3-5% was found there.
      
The compound is not used as a C source by the bacterium, either.
      
The molecular size of pullulan from the combined olive oil-sucrose fermentation was slightly lower (1.1 X 106) than that of conventional fermentation with sucrose as a single C source (1.4 X 106).
      
With malic acid as the sole C source, two strains (Nos.
      
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  其他


Skim serum is an industrial effluent from rubber factories. It is of higher BOD and contains about 1% protein and 0.05-0.3% ammoniacal nitrogen, etc. The effluent was used to culture Geotrichum candidum as SCP(single-cell protein) feed, thus reducing environmental pollution. This paper describes the optimum technical conditions for culturing Geotrichum candidum with skim serum effluent.The experiments were carried out on a multifactorial and orthogonal design. The results showed that, among the four strains...

Skim serum is an industrial effluent from rubber factories. It is of higher BOD and contains about 1% protein and 0.05-0.3% ammoniacal nitrogen, etc. The effluent was used to culture Geotrichum candidum as SCP(single-cell protein) feed, thus reducing environmental pollution. This paper describes the optimum technical conditions for culturing Geotrichum candidum with skim serum effluent.The experiments were carried out on a multifactorial and orthogonal design. The results showed that, among the four strains used, Geotrichum candidum Shuntang No.2 recorded the highest VPE. When 1-1.5% of carbon source was added to the skim serum medium, Geotrichum candidum grew very well on it and the cell yield(dry wt.) accounted for 1.5%. Besides, the reducing sugar consumption was lower. The ratio of cell (on dry wt. basis) to reducing sugar consumption was 1:0.6. The residual sugar in the medium was less than 0.1%.We came to the conclusion that the optimum technical conditions for culturing Geotrichum candidum with skim serum effluent are: molasses used as carbon source, carbon-nitrogen ratio (C:N)-10:1, aeration=1:1 (V V), pH 5.5, fermenting duration 9-10 hours, fermenting temperature 29°—/31℃, stirring speed 450 rpm.In addtion to 53.85% of crude protein, 3.65% of fat and 6.34% of nucleic acid, Geotrichum candidum grown on this medium contained 18 amino acids and vitamin B, but its aflatoxin B_1 was less than 5ppb. These cells are highly nutritious. They may be used as SCP feed for poultry and livestock, or for extracting nucleic acid, thereby reducing BOD.

胶清废水系天然橡胶初加工时的一种工业废水,具有较高的生化需氧量(BOD),其中约含有1%蛋白质,0.05~0.3%氨态氮等。我们曾利用这种废水培养白地霉(Geotrichum candidum)作为单细胞蛋白饲料,从而减少对环境污染。本文叙述了利用胶清废水培养白地霉的最适工艺条件。试验采用多因子正交设计法。试验结果表明:在采用的四个菌株中以顺糖2号自地霉的容积生产效率(VPE)最高,在培养基中仅需添加1~1.5%的碳源,该菌株在胶清废水中就能良好生长,干菌体产量达1.52%。此外,耗糖少(菌体与还原糖之比为1:0.6),残糖低(0.1%以下)。试验表明,最适培养条件是:糖蜜作碳源、碳氮比10:1,通气量(V/V)1:1,pH值5.5,发酵时间9~10小时,温度29~31℃,搅拌速度450转/分等。其菌体含粗蛋白质53.85%,脂肪3.65%,核酸6.34%,并含有18种氨基酸,具有赖氨酸等8种营养必需的氨基酸,和乙族维生素等,黄曲霉毒素B_1<5 ppb。所得菌体营养丰富,不仅供作禽畜的单细胞蛋白质饲料,并可抽提核酸,同时可减少胶清废水的生化需氧量(BOD)。

In the culture medium with such suitable carbon sources as dextrin and soluble starch and such suitable nitrogen ones as soy bean extract, the strain Bacillus subtilis KO2 can produce saccharogenic α-amylase at the maximum on the third day. Pure enzyme was obtained by using the salt fractionation with ammonium sulphate, the ion-exchange chromatography and the gel filtration. A series of study on the pure enzyme indicate that the isoelectric point was pH 5.16, the pH stability range was between pH 5.0 and 8.5,...

In the culture medium with such suitable carbon sources as dextrin and soluble starch and such suitable nitrogen ones as soy bean extract, the strain Bacillus subtilis KO2 can produce saccharogenic α-amylase at the maximum on the third day. Pure enzyme was obtained by using the salt fractionation with ammonium sulphate, the ion-exchange chromatography and the gel filtration. A series of study on the pure enzyme indicate that the isoelectric point was pH 5.16, the pH stability range was between pH 5.0 and 8.5, the heat stability range was below 40—50℃, the optimum pH for action was pH 5.5, and the optimum temperature was 66℃. Its molecular weight was estimated to be 42,000~45,000 by using the methods of gel filtration, high performence liquid chromatography, SDS-electrophoresis and ultracentrifugation. The enzyme protein was composed with 396 amino aeid residues and the percentage and the amount of every, amino acid were assayed. The kinetic indices were that the Km value for soluble starch was 0.057 and the maximum velocity was 1.3 micromole per minute per milliliter pre unit of the enzyme. Comparison of this enzyme with liquefying α-amylase, glucoamylase in the action pattern on several subsrates showed that the enzyme BSA has special action mechanism. The part Ⅰ here deals with the production and the purification of the enzyme.

枯草芽孢杆菌KO_2在合适的碳源,如糊精、可溶性淀粉及氮源,如豆饼浸出液的培养液中三天能达产糖化型α-淀粉酶高峰。使用硫酸铵分级盐析,离交色谱,凝胶过滤可获得纯净的酶。对此纯化的酶的研究表明:该酶的等电点为pH5.16,pH稳定范围5.0~8.5,温度稳定范围40~50℃以下,最适作用pH为5.5,最适作用温度66℃,分子量经凝胶过滤、高压液相色谱、SDS电泳及超速离心分析测得为42,000~45,000,由396氨基酸残基构成并确定了各种氨基酸的百分比及数量,对于可溶性淀粉的米氏常数为0.057,最大速度V_(max)=1.3微克分子/分钟·亳升·单位酶活。比较此酶与液化型α-淀粉酶、糖化酶对基质的作用表明,此酶具有独特的作用机理。

Aspergillus niger 505 was treated with r ray and diethyl sulfate ( DES ) in the present study, and a strain A. niger UCD69 was obtained, which was superior to the parent by more than 3.7 times when glucose oxidase activity was concerned. The results showed DES was a more effective mutagen than r-ray in increasing the activity of glucose oxidase by A. niger.

本试验以黑曲霉505菌株为出发菌株,经γ-射线和硫酸二乙酯(DES)处理后,选出一株产萄葡糖氧化酶多的黑曲霉CUD69菌株,其葡萄糖氧化酶(GOD)活力较出发菌株高3.7倍.另外从提高葡萄糖氧化酶活的观点来看,试验结果表明,DES的诱变效果较γ-射线的好.对菌体生长和其产生葡萄糖氧化酶条件的试验结果表明,碳源种类、氮源种类及其配比,培养液中接入的孢子量、通气量等因素对菌体生长和产GOD影响很大.选出的最适培养条件为:10%蔗糖,0.1%蛋白胨,0.01%MgSO_4·7H_2O,0.04%(NH_4)_2 HPO_4;在每个500ml三角瓶中装100ml培养液时,接种孢子量以200~300万个为宜,摇瓶培养32~34小时,温度30℃.最后在用上述条件下培养的黑曲霉菌丝作了发酵葡萄糖直接生产葡萄糖酸—δ—内酯(GDL)的试验,证明这种方法是可行的,所得产品的外观与性质均与美进口的GDL一致,产品收率为初糖重量的45.1%.

 
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