While deproteinizing,the percentage of deproteinization reaches 97.4% under the conditions of sample(trichloromethane+2-butanol)(v∶v)=1∶1,trichloromethane/2-butanol(v∶v)=4∶1 and extraction for 60 minutes.
Methods:TLC scanning was used to determine the content of ursolic acid, using the cyclohexane: trichloromethane: ethyl acetate: glacial acetic acid(20∶5∶8∶0.5) as the developing system, 10% sulfuric acid-ethanol solution as the spray reagent, the detected by dual wavelength scanning (λ S=530nm, λ R=700nm).
Genome DNA was extracted from the white blood cell with the phenol trichlormethane method; The paraoxonase 2 gene C311S polymorphism of blood DNA was detected with polymerase chain reaction restrictive fragment length polymorphism(PCR RFLP),and then compared after the genotype frequency at paraoxonase 2 gene site was searched in domestic and oversea literatures.
In this paper, the effecting factors on chloroform produced(such as reaction time , temperature, initial pH,TOC, and so on )was studied and the predicatable mathematical models forming trichlormethane in potassium permanganate per-ox-idation were presented by using linear regression method.
It has studied the extractive effect and physical character of trichlormethane ,1,2-dichloroethane, trichloroethylene, dichloromethane, ethyl acetate and carbontetrachloride. In the same time, the other effecting factors on the extraction effect have been discussed. The experiment results have showed, trichlormethane has the highest effect of extraction for furfural , and 1,2-dichloroethane is in the next place.
It was demonstrated that zanthoxylum trichlormethane extracts(ZTE) had strong scabicide activity in animal experiment. The results were dealed with by computer. The LD50 of ZTE contacting with Sarcoptes scabiei in 8 hours was 9.311791,and the scabicide rate of ZTE was highly corelated with its concentration and contact time.
Most of thorium was removed from the title ore in the 6V/V% HCl solution using cupferron—choroform, and uranium and remainder thorium eliminated from 10 V/V% HCl solution by diphenyl guanidine(DPG)—Arsenazo Ⅲ—n-butanol.
Results showed: modified choroform extraction method is suitable for the extraction of duck and goose yolk immunogolobulins, and the purified immunloglobulins had high purity (>90%) and activity (> 1:64);
aureus in lower concentration of chloroform extract.
This was also related to the presence in chloroform of chlorocarbonic acid ethyl ester that reacted with roquefortine.
Adding 2% chloroform (activating agent), autolysis for 5-10 h, and sonication of autolysates allowed us to obtain mixtures balanced in lysine, methionine, and tryptophan and containing up to 90% free amino acids.
We studied the preparation of polymeric films formed from solutions of poly-3-hydroxybutyrate and poly-ε-caprolactone in chloroform and methylene chloride.
144 purified from Escherichia coli effectively degrades chloroform-treated P.