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胞质游离钙
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  cytoplasmic free calcium
     AIM Aim to study the effect of PLC on human platelet cytoplasmic free calcium concentration, and then to explore the mechanism of PLC anti aggregation to platelet.
     目的 测定磷酯酶C(phospholipaseC ,简称PLC ,下同 )对静息状态和激活状态下人血小板胞质游离钙离子浓度的影响 ,从而进一步阐明PLC抗血小板聚集作用的机制。
短句来源
  intracellular free calcium
     Intracellular free calcium concentration was significantly increased by 10 μmol/L NMDA, which was remarkably inhibited by the liquid extract.
     10μmol/LNMDA可明显增加海马神经元胞质游离钙浓度。
短句来源
     AIM: To investigate the effects of octreotide (OCT) on the contraction of endothelin-1(ET-1)-stimulated hepatic stellate cell (HSC), and to explore the changes of intracellular free calcium concentration ([Ca2+]i).
     目的:探讨奥曲肽(OCT)对内皮素-1(ET-1)刺激的肝星状细胞(HSC)收缩的影响,以及此过程中胞质游离钙([Ca2+]i)的变化.
短句来源
     AIM: To gain more insight into the mechanism of neuronal injury in phenylketonuria patients and to investigate the effect of phenylalanine on intracellular free calcium concentration ( i) of embryonic rat cortical neurons.
     目的 :探讨苯丙酮尿症患者脑损伤的病理生理机制 ,分析苯丙氨酸和胞质游离钙有无关系 . 方法 :用钙成像技术检测氨基酸及其衍生物对胚鼠皮层未成熟神经元胞质游离钙浓度的影响 .
短句来源
     The effects of NMDA on intracellular free calcium was abolished by pretreatment of the cells with MK-801 for 10 min, whereas the liquid extract significantly lowered the antagonizing effect of MK-801. Conclusion Lishi No.
     李氏5号水提液本身可轻度增加胞质游离钙浓度(与正常组比较差异不显著),但明显抑制NMDA导致的胞质游离钙浓度的升高; NMDA阻断剂MK-801明显拮抗NMDA导致胞质游离钙升高的效应,但李氏5号水提液预处理后,MK-801拮抗NMDA的效应显著降低。
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  “胞质游离钙”译为未确定词的双语例句
     The increase of cytoplasmic free Ca2+ ([ Ca2+]i) is involved in the signaling network triggered by some stimuli such as biotic and abiotic stress and phytohormones as well.
     植物受到生物和非生物胁迫以及激素刺激时,胞质游离钙离子浓度([Ca~(2+)]_i)升高。
短句来源
     Although there is a relationship between JA stimulation and [Ca2+]i increase, very few evidence has been obtained with a direct measurement of [ Ca2+]i after JA treatment.
     尽管JA与胞质游离钙离子有关,但直接测定细胞[Ca~(2+)]_i的变化还鲜见报道。
短句来源
     THE RELATIONSHIP BETWEEN CYTOSOLIC Ca~(2+) AND TWO KINDS OF GRANULE NUMBER IN ACTIVATED PLATELETS
     血小板激活时胞质游离钙浓度与两种颗粒数变化的相关性
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     Effect of two different cardioplegia on calcium concentration of isolated cardiomyocytes of rat
     极化型与去极化型心脏停搏液对离体大鼠心肌细胞胞质游离钙离子含量的影响
短句来源
     Methods Cytosolic free Ca 2+ concentration([Ca 2+ ] i) in isolated pancreatic acinar cells,and plasma prostanoid production (thromboxane B 2,6 keto prostaglandin F 1α) were studied in two models of pancreatitis in rats. AEP was induced by pancreatic duct ligation and exocrine stimulation. ANP was induced same as AEP but with a larger dose of Dextran 110(500mg/kg) intravenously.
     方法 采用胰管结扎、外分泌刺激诱发大鼠AEP ,在AEP模型基础上静脉注射大剂量高分子右旋糖酐Dextran 110 (5 0 0mg/kg)诱发ANP ,应用荧光探针Fura2 /AM直接测定胰腺泡细胞胞质游离钙离子浓度([Ca2 + ]i) ,并观察血浆前列腺素类 :血栓素A2 (TXA2 )、前列环素 (PGI2 )的变化。
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  相似匹配句对
     ANTIHYPERTENSIVE DRUGS AND PLATELETS IONIZED CALCIUM
     抗高血压药与血小板游离
短句来源
     Clinical value of determination of free calcium in plasma
     血浆游离测定的临床意义
短句来源
     Effect of octreotide on regulation of intracellular free Ca~(2+) concentration of hepatic stellate cells in rats
     奥曲肽对大鼠肝星状细胞胞质游离及细胞增殖的影响
短句来源
     Intracellular free calcium concentration was significantly increased by 10 μmol/L NMDA, which was remarkably inhibited by the liquid extract.
     10μmol/LNMDA可明显增加海马神经元胞质游离浓度。
短句来源
     THE RELATIONSHIP BETWEEN CYTOSOLIC Ca~(2+) AND TWO KINDS OF GRANULE NUMBER IN ACTIVATED PLATELETS
     血小板激活时胞质游离浓度与两种颗粒数变化的相关性
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  cytoplasmic free calcium
Effect of interleukin-1β on the elevation of cytoplasmic free calcium of the cultured hippocampal neurons induced by L-glutamate
      
Oscillations in cytoplasmic free calcium concentration in human pancreatic islets from subjects with normal and impaired glucose
      
This strongly suggests that there is no correlation between maximal stimulation of cytoplasmic free calcium, and maximal stimulation of migration.
      
The mobilization of cytoplasmic free calcium is also normal.
      
Cytoplasmic free calcium has been observed in fura 2-loaded stria vascularis of the guinea pig.
      
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  intracellular free calcium
Involvement of cAMP-Dependent protein kinase and intracellular free calcium ions in regulation of ovulation of the follicle-encl
      
It was concluded that cAMP-dependent protein kinase and intracellular free calcium ions were involved as second messengers in the gonadotropin regulation not only in maturation of amphibian oocytes but also in ovulation.
      
on intracellular free calcium in cultured vascular smooth muscle cells of
      
Both hypoxic endothelial cell conditioned medium and hypoxia elevate intracellular free calcium in pulmonary artery smooth muscl
      
The concentration of intracellular free calcium ([Ca2+]i) of single cultured ventricular myocyte was determined by using EPC-9 light-electricity measurement system.
      
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The free calcium in single mouse peritoneal macrophages was labelled with fluorescence dye Indo-1 and its dynamic changes were observed with Adherent Cell Analysis and Sorting Cytometer (ACAS)570. The level of intracellular free calcium elevated rapidly after the stimulation of lipopolysaccharide (LPS) from E. coli O55: B5 with a peak at 500 s. After the addition of LPS into the macrophages processed with polydatin,no obvious elevation of intracellular calcium ion level could be seen. Because the cells were...

The free calcium in single mouse peritoneal macrophages was labelled with fluorescence dye Indo-1 and its dynamic changes were observed with Adherent Cell Analysis and Sorting Cytometer (ACAS)570. The level of intracellular free calcium elevated rapidly after the stimulation of lipopolysaccharide (LPS) from E. coli O55: B5 with a peak at 500 s. After the addition of LPS into the macrophages processed with polydatin,no obvious elevation of intracellular calcium ion level could be seen. Because the cells were in a calcium-free environment,intracellular Ca2+ elevation was unlikely due to extracellular calcium influx,while LPS interfered with the regulation of calcium pool within the macrophages. Polydatin might protect some regulatory mechanisms of cytosolic Calcium in intracellular calcium pool,and so avoid cytosolic Ca2+ elevation.

用荧光染料Indo-1标记小鼠腹腔巨噬细胞内游离钙,在粘附式细胞仪检测下观察细胞内游离钙的动态变化,在大肠杆菌O55:B5内毒素刺激下细胞内游离钙迅速升高,于第400s达峰值.经虎杖甙处理的巨噬细胞加入内毒素后,胞质游离钙无明显增高.因细胞浴于无钙环境,故胞内游离钙升高不是由于细胞外的Ca2+进入细胞,而是由于内毒素影响胞内钙库调节功能.虎杖甙可能保护胞内钙库中胞质钙的某种调节机制,从而避免胞质游离钙升高.

Objective The aim of this work was to establish a relationship between calcium homeostasis and prostanoids in the conversion of acute edematous pancreatitis(AEP) to necrotizing pancreatitis(ANP) in rats. Methods Cytosolic free Ca 2+ concentration([Ca 2+ ] i) in isolated pancreatic acinar cells,and plasma prostanoid production (thromboxane B 2,6 keto prostaglandin F 1α) were studied in two models of pancreatitis in rats.AEP was induced by pancreatic duct ligation and exocrine stimulation. ANP...

Objective The aim of this work was to establish a relationship between calcium homeostasis and prostanoids in the conversion of acute edematous pancreatitis(AEP) to necrotizing pancreatitis(ANP) in rats. Methods Cytosolic free Ca 2+ concentration([Ca 2+ ] i) in isolated pancreatic acinar cells,and plasma prostanoid production (thromboxane B 2,6 keto prostaglandin F 1α) were studied in two models of pancreatitis in rats.AEP was induced by pancreatic duct ligation and exocrine stimulation. ANP was induced same as AEP but with a larger dose of Dextran 110(500mg/kg) intravenously. Results The results showed that pancreatic acinar cell [Ca 2+ ] i increased consistently after induction of ANP,and plasma TXB 2 increased significantly with respect to AEP.Conclusion The present results indicate that calcium homeostsis may play an important role and may be responsible,in part,for prostanoid enhanced biosynthesis in the conversion of AEP to ANP in rats.

目的 探讨钙稳态与前列腺素类异常在急性水肿性胰腺炎 (AEP)向坏死性胰腺炎 (ANP)发展过程中的作用。方法 采用胰管结扎、外分泌刺激诱发大鼠AEP ,在AEP模型基础上静脉注射大剂量高分子右旋糖酐Dextran 110 (5 0 0mg/kg)诱发ANP ,应用荧光探针Fura2 /AM直接测定胰腺泡细胞胞质游离钙离子浓度([Ca2 + ]i) ,并观察血浆前列腺素类 :血栓素A2 (TXA2 )、前列环素 (PGI2 )的变化。结果 ANP模型后腺泡细胞胞质游离钙离子浓度呈持续增高 (P <0 .0 5 ) ,同时血浆TXA2 水平也明显增高 (P <0 .0 5 ) ,而血浆 6 keto PGF1α无明显变化 (P >0 .0 5 )。结论 局部缺血时钙稳态失衡在AEP向ANP发展过程中可能是引起前列腺素类异常增加的原因

AIM:To investigate whether emodin has any effects on circular smooth muscle cells of rat colon and to examine the underlying mechanisms. METHODS:Smooth muscle cells were isolated from the circular muscle layers of Wistar rat colon and cell length was measured by computerized image micrometry.Intra- cellular Ca~(2+)([Ca~(2+)]i)signaling was studied in smooth muscle cells using Ca~(2+)indicator Fluo-3 AM by laser-scanning confocal microscopy. RESULTS:Emodin dose-dependently induced smooth muscle cells contraction,caused...

AIM:To investigate whether emodin has any effects on circular smooth muscle cells of rat colon and to examine the underlying mechanisms. METHODS:Smooth muscle cells were isolated from the circular muscle layers of Wistar rat colon and cell length was measured by computerized image micrometry.Intra- cellular Ca~(2+)([Ca~(2+)]i)signaling was studied in smooth muscle cells using Ca~(2+)indicator Fluo-3 AM by laser-scanning confocal microscopy. RESULTS:Emodin dose-dependently induced smooth muscle cells contraction,caused a large,transient increase in[Ca~(2+)]i followed by a sustained elevation in[Ca~(2+)]i.Emo- din-induced increase in[Ca~(2+)]i was unaffected by nifedipine,a votage-gated Ca~(2+)-channel antagonist,and the sustained phase of rising of[Ca~(2+)]i was attenuated by extracellular Ca~(2+)removal with EGTA solution.Inhibiting Ca~(2+)release from ryanodine-sensitive intracellular stores by ryanodine reduced the peak increase in [Ca~(2+)i.However, the application of heparine,an antagonist of IP_3R,nearly abolished the peak increase in [Ca~(2+)]i induced by emodin. CONCLUSION:Emodin has direct excitatory effect on cir- cular smooth muscle cells from rat colon and its effect is mediated through Ca~(2+)-dependent pathways.Furthermore, emodin-induced peak[Ca~(2+)]i increase may be attributable to the Ca~(2+)release from IP_3 sensitive stores,which pro- motes Ca~(2+)release from ryanodine-sensitive stores through CICR mechanism.Additionally,Ca~(2+)influx from extracellular medium contributes to the sustained increase in[Ca~(2+)]i.

目的:观测大黄素对 Wistar 大鼠结肠环行平滑肌细胞胞质游离钙水平([Ca~(2+)]i)的影响,并探讨机制.方法:酶解法分离结肠环行平滑肌细胞,分别采用图像分析系统和激光扫描共聚焦显微镜观测细胞长度和[Ca~(2+)]i 的变化.结果:大黄素可收缩大鼠结肠环行平滑肌细胞,也可浓度依赖性升高[Ca~(2+)]i;大黄素作用后,[Ca~(2+)]i 迅速升高达到值([Ca~(2+)]i_(peak)),而后下降至平台期([Ca~(2+)]i_(Sustain),仍高于静息值).Nifedipine 对大黄素的钙动员作用没有明显影响,EGTA明显降低[Ca~(2+)]i_(Sustian).Heparine 几乎完全抑制[Ca~(2+)]i_(Peak),Ryanodine 明显抑制[Ca~(2+)]i_(Peak).结论:大黄素通过升高[Ca~(2+)]i 收缩结肠环行平滑肌细胞.大黄素升高[Ca~(2+)]i 的机制为:活化 IP3受体释放细胞内钙,进一步以 CICR 方式促进 RYR 受体开放,共同作用升高[Ca~(2+)]i,细胞外钙内流参与平台期[Ca~(2+)]i 的升高.

 
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