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植株再生
相关语句
  plant regeneration
    High Frequency Plant Regeneration of Brassica campestris ssp. Chinensis from Cotyledon Segments
    两步法高频率小白菜子叶离体植株再生体系的建立
短句来源
  pla nt regeneration
    High Frequency Plant Regeneration of Brassica campestris ssp. Chinensis from Cotyledon Segments
    两步法高频率小白菜子叶离体植株再生体系的建立
短句来源
  “植株再生”译为未确定词的双语例句
    Regeneration of HNP Transgenic Alfalfa Plants by Agrobacterium Mediated Gene Transfer
    苜蓿高含硫氨基酸蛋白转基因植株再生
短句来源
  相似匹配句对
    STUDIES ON TISSUE AND CELL CULTURE AND PLANT REGENERATION OF Ilex kudingcha
    苦丁茶体细胞组织培养再生植株的研究
短句来源
    PRODUCTION OF PLANTS FROM SOMATIC HYBRIDIZATION BETWEEN COMMON WHEAT AND MAIZE(ZEA MAYS L)
    普通小麦与玉米的体细胞杂交再生完整植株
短句来源
    Progress in the Studies of Grape Regeneration System
    葡萄再生系统研究进展
短句来源
    (f) The technique for the use of renewable energy resources;
    ⑥再生能源利用技术;
短句来源
    gene and SBTi gene were obtained.
    和SBTi双基因的植株系。
短句来源
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  plant regeneration
Effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb
      
The effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb.
      
The results supported the published data that individual androgenesis parameters (embryogenesis, total plant regeneration, green plant regeneration) are controlled by different genetic mechanisms.
      
The Lr19 translocation was shown to affect the induction of embryogenesis and green plant regeneration.
      
The effect of medium composition (phytohormones and carbohydrates) on the frequency of the formation of a morphogenic callus competent for plant regeneration has been determined.
      
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Hypocotyl protoplasts were isolated with enzyme solution,and used as explants for protoplast culture.The division frequency was about 50%,the frequency of plant regeneration was 100 %.The transient gene expression with cotyledon protoplasts was studied, based on the study of transient gene expression and protoplast culture,The protoplasts isolated were treated with bacterial plasmid DNA(pBI222),and cultured at a density of 5×10 ̄4/ml.After 10-15 days,they were selected by adding 25μg/ml hygromycine. One month...

Hypocotyl protoplasts were isolated with enzyme solution,and used as explants for protoplast culture.The division frequency was about 50%,the frequency of plant regeneration was 100 %.The transient gene expression with cotyledon protoplasts was studied, based on the study of transient gene expression and protoplast culture,The protoplasts isolated were treated with bacterial plasmid DNA(pBI222),and cultured at a density of 5×10 ̄4/ml.After 10-15 days,they were selected by adding 25μg/ml hygromycine. One month later,a few calli observed were transferred onto the solid proliferation medium with 50μg/ml hygromycine. After transferred onto the differentiation rooting medium, the hygromycine-resistant plants were obtained.The Whole plants were transplanted into pots and grew well.The frequency of regeneration was 4×10 ̄(-5). The excised leaves of the transgenic plants were used as explants for Southern blot analysis.It was confirmed that HPT gene had been stably integrated into the chromosomal genome of Orychophragmus violaceus. The transformation frequency of hypocotyl protoplasts was 10(-5).

采用诸葛菜无菌苗的下胚轴组织为材料,分离原生质体,在原生质体培养基中作液体浅层暗培养,植板率为5%,植株再生频率为100%。作者进而开展了遗传转化研究。为研究PEG介导转化诸葛菜原生质体的影响因素,通过瞬间表达,实验了PEG法转化子叶原生质体的过程,在此基础上将分离纯化后的原生质体与带HPT基因的质粒DNA(pBI222)混合,HPT基因作选择标记,PEG介导转化;重新收集转化后的原生质体,以5×104/ml的密度在原生质体培养基中作浅层培养;培养10—15天后用25mg/L的潮霉素(hygromycin)进行筛选,一月后出现少量细胞团,转入含潮霉素50mg/L的扩增培养基扩增愈伤组织,进而转入含50—100mg/L潮霉素的分化培养基诱导分化成苗,分化率为100%,转入生根培养基中生根成完整植株。抗性植株再生率为4×10(-5)。在获得再生转基因植株后,以再生植株叶片为材料,进行Southernblot分子杂交,证实外源基因已稳定整合到植物基因组中并表达,再生转基因植株频率为10(-5)。国内外首次转化诸葛菜属植物原生质体获得成功。

A test on induction of leaf callus and regeneration of plants of white poplars was completed by using mixtures of various growth regulators such as NAA,2,4-D,IAA,IBA and 6-BA.Under the action of the mixtures of 6-BA0.5mg/L and NAA0.2-2.0mg/L,2,4-D2.0mg/L or IAA2.0mg/L respectively,the induction rates of leaf callus all could reach 100%and under the action of the mixture of 6-BA 1.0mg/L and NAA0.2mg/L the shoot induction rate was 95%.We also found...

A test on induction of leaf callus and regeneration of plants of white poplars was completed by using mixtures of various growth regulators such as NAA,2,4-D,IAA,IBA and 6-BA.Under the action of the mixtures of 6-BA0.5mg/L and NAA0.2-2.0mg/L,2,4-D2.0mg/L or IAA2.0mg/L respectively,the induction rates of leaf callus all could reach 100%and under the action of the mixture of 6-BA 1.0mg/L and NAA0.2mg/L the shoot induction rate was 95%.We also found that NAA and IBA of a proper concentration can help test tube seedlings to take root.

利用NAA、2,4-D、IAA、IBA、6-BA5种生长调节剂的不同浓度和不同组合配比,完成了对毛白杨叶愈伤组织诱导和完整植株的再生。在6-BA0.5mg/L分别和NAA0.2~2.0mg/L、2,4-D2.0mg/L、IAA2.0mg/L共同作用下,叶愈伤组织诱导率均能达到100%,愈伤组织在6-BA1.0mg/L和NAA0.2mg/L作用下芽诱导率为85%,而适当浓度的NAA和IBA有促进试管苗生根的作用

Gypsophila paniculata,as a receptor, was genetically transformed by method of co-culture with A. tumefaciens. After infecion,crown gall occurred on receptor plant. By comparing the occurrence and shape of gall with the regeneration of transformed plant,it is showed that the occurrence of crown gall is essential for genetic transformation.

用根癌农杆菌(A.tumefacien)以共培养方法对受体植物丝石竹(Gypsophilapaniculata)进行基因转化,感染后引起受体植物冠瘿瘤的发生。对瘤的发生、瘤的形态,与转化植株的再生进行比较证明,冠瘿瘤的发生对基因转化是必需的。

 
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