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卡介苗     
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  bcg
    1.Studying on the Molecular Chaperone Activity and Associated Function of Small Heat Shock Protein, Hsp16.3, from Mycobacterium Tuberculosis 2.Studying on Gene Knock Out in Mycobacterium Bovis BCG
    一、结核分枝杆菌Hsp16.3分子伴侣活性及其相关功能的研究 二、卡介苗菌MDP1基因敲除的研究
短句来源
    Impact of Neonatal BCG Vaccination and RSV Infection on Immune Status and Experimental Asthma in Mice
    小鼠卡介苗新生期接种和呼吸道合胞病毒感染对免疫状态和实验性哮喘的影响
短句来源
    Expression of Secreted Ag85A of BCG in Tomato
    卡介苗分泌型Ag85A在番茄中表达的研究
短句来源
    MICROWAVE (2450 MHz) WITH LOW INTENSITY EFFECTS ON IMMUNE SYSTEM IN PREIMMUNIZED RATS WITH BCG
    低强度微波(2450MHZ)对卡介苗预免疫大鼠免疫系统的影响
短句来源
    Theoretical and methodological aspects of BCG vaccine from the discovery of Calmette and Guerin to molecular biology. A review
    卡介苗的理论和方法学两个方面的情况综述——从Calmette和Guérin的发现到分子生物学
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  bcg vaccine
    Theoretical and methodological aspects of BCG vaccine from the discovery of Calmette and Guerin to molecular biology. A review
    卡介苗的理论和方法学两个方面的情况综述——从Calmette和Guérin的发现到分子生物学
短句来源
    Construction, Expression and Identification of a Recombinant BCG Vaccine Encoding Human Mycobacterium tuberculosis Heat Shock Protein 65
    人结核杆菌热休克蛋白65重组卡介苗疫苗的构建、表达及鉴定
短句来源
    The Relationship between Anti-tuberculosis Effect and CD4~+CD8~+ Double Positive(DP)T Lymphocytes Subset in the Rats with BCG Vaccine
    卡介苗免疫大鼠的抗结核发病和CD4~+CD8~+双阳T细胞
短句来源
    Construction of A Recombinant BCG Vaccine Expressing the Secretory Form Fusion Protein 85B-ESAT-6
    分泌性表达融合蛋白85B-ESAT-6重组卡介苗的构建和表达
短句来源
    Construction and identification of Ag85a-recombinant BCG vaccine
    Ag85a-卡介苗重组疫苗的构建及鉴定
短句来源
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  bacillus calmette-guerin
    Release of Nitric Oxide Release From the U-937 and THP-1 Macrophages Induced by Bacillus Calmette-Guerin
    卡介苗诱导U-937及THP-1巨噬细胞释放一氧化氮的实验研究
短句来源
    Bacillus Calmette-Guerin (BCG) genomic DNA in Escherichia coli was cloned by cloning sau3A1 cleaved BCG DNA fragments 2-9 kb into BamHl-digested.
    以质粒pUC19为载体克隆了卡介苗(即卡介菌,BCG)DNA. Sau3Al部分酶解BCG DNA,通过琼脂糖电泳分离2~9Kb DNA片段.
短句来源
    Objective To observe the influence of Bacillus Calmette-Guerin(BCG)'s secretory protein to human mononuclear cells(MCs).
    目的初步探讨卡介苗(Bacillus Calmette-Guerin,BCG)分泌性蛋白在体外培养试验中对人单个核细胞的刺激效应。
短句来源
    coli as a GST fusion protein (rSj14/GST) and used to vaccinate outbred Kunming mice by using complete Freund’s adjuvant (FCA)/ incomplete Freund’s adjuvant (FIA), Bacillus Calmette-Guerin (BCG) and the immunostimulating complex (ISCOM) as adjuvant respectively.
    coli表达日本血吸虫脂肪酸结合蛋白基因,制备日本血吸虫脂肪酸结合蛋白与GST的重组融合蛋白(rSj14/GST),分别以福氏完全佐剂(FCA)/福氏不完全佐剂(FIA)、卡介苗(BCG)和免疫刺激复合物(ISCOM)作为佐剂免疫昆明系小鼠,比较攻击感染后的减虫效果。
短句来源
    Establishment of chronic bronchitis rat model by combined utilization of Bacillus Calmette-Guerin (BCG) and lipopolysaccharide (LPS)
    脂多糖联合卡介苗诱发大鼠慢性支气管炎模型的构建
短句来源
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  bacille calmette guerin
    Construction and Identification of Bacille Calmette Guerin Recombinant ShuttlePlasmid Secreting IFN-α2a
    卡介苗穿梭质粒pMSIFN-α2a的构建与鉴定
短句来源
    TNFα-monoclonal antibody inhibited NO production induced by bacterial lipopolysaccharide (LPS) in Kupffer's cells, but did not affect that by stimulated bacille calmette guerin (BCG).
    肿瘤坏死因子(TNFα)单克隆抗体可拮抗枯否细胞由细菌脂多糖(LPS)刺激所致NO生成的增加,而对卡介苗(BCG)所致NO生成无显著影响。
短句来源
    Methods α antigen(α Ag) signal sequence and esat 6 gene were amplified from the genome of Bacille Calmette Guerin (BCG) and Mycobacterium tuberculosis by PCR respectively.
    方法 分别以卡介苗(BCG)和结核分枝杆菌 H37Rv株基因组 DNA为模板 ,通过 PCR扩增得到约 117bp的 BCGα抗原 (α- Ag)信号肽序列和 2 85 bp的结核杆菌 esat- 6基因序列。
短句来源
    Inoculation of bacille calmette guerin combined with intraperitoneal adenosine injection enhances antibacterial effect of macrohage against Mycobacterium tuberculosis in mice
    卡介苗配用腺苷接种提高巨噬细胞抗结核杆菌效能的研究
短句来源
    Objective: To study the method of electro transformation of Mycobacteria including M.Smegmatis mc 2155 and Bacille Calmette Guerin (BCG), and the factors that affect transformation efficiency.
    目的 :探讨卡介苗 (BacilleCalmette Guerin ,BCG)分枝杆菌的电穿孔转化方法及其影响因素。
短句来源
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      bcg
    Presently, one of the most potent immunotherapies is the application of bacillus Calmette Guerin (BCG) to prevent recurrences of the superficial bladder cancer.
          
    Therefore, current studies aim at developing recombinant BCG (rBCG) strains secreting Th1-like cytokines to improve the effectiveness of the therapy.
          
    Peripheral blood monocytes (PBMC) were stimulated by recombinant BCG and transformed into bacilli Calmette-Guerin activated killer (BAK) cells, and the effect of anticancer BAK cells was studied.
          
    Recombinant IFN-α-2b-BCG, wild-type BCG (wBCG), wild-type BCG and IFN-α-2b were coincubated with PBMCs respectively in vitro, and the proliferation of PBMC was detected with MTT in different time.
          
    The result of MTT showed that the proliferation of PBMC in the recombinant BCG group was more powerful than in the other two groups (P >amp;lt; 0.05).
          
    更多          
      bcg vaccine
    Construction, expression and identification of a recombinant BCG vaccine encoding human mycobacterium tuberculosis heat shock pr
          
    The recombinants were electroporated into BCG to construct the recombinant BCG vaccine and induced by heating.
          
    Recurrent soft tissue infection following accidental inoculation with BCG vaccine
          
    Antitumor activity of two BCG vaccine preparations against the lewis lung carcinoma in mice
          
    Two BCG vaccine preparations were prepared following different production methods.
          
    更多          
      bacillus calmette-guerin
    The expression of foreign gene,Schistosoma Japonicum 26 ku antigen (Sj26GST),in Bacillus Calmette-Guerin (BCG),Mycobacterium CM.
          
    Bacillus Calmette-Guerin (BCG) instillation in the bladder results in a massive local inflammatory response that has secondary antitumor properties.
          
    Analysis of mucosal bladder leucocyte subpopulations in patients treated with intravesical Bacillus Calmette-Guerin
          
    Cytokine production by the human bladder carcinoma cell line T24 in the presence of bacillus Calmette-Guerin (BCG)
          
    The study was initiated as an in vitro approach to the situation existing during intravesical bacillus Calmette-Guerin (BCG) instillation in patients with superficial bladder cancer.
          
    更多          
      bacille calmette guerin
    We have used recombinant Bacille Calmette Guerin (BCG) strains overexpressing Ag 85A and Ag 85C to immunize BALB/c mice to investigate the immunogenicity of these strains.
          
    Cloning and expression of a candidate malarial epitope in bacille Calmette Guerin
          


    In this report inulin was adapted to absorb C3 complement fromnormal human sera. Since then C3-inulin complex has been used for thepreparation of anti-C3 serum. The immunization procedure was based uponthe fact that living BCG was first inoculated for the purpose of activa-ting the immune system. After the initial activation, 2 injections of C3-inulin complex with complete Freund adjuvant were made subcutaneously,intramuscularly and intra-glandularly on multiple sites of the rabbit.By means of this method, we...

    In this report inulin was adapted to absorb C3 complement fromnormal human sera. Since then C3-inulin complex has been used for thepreparation of anti-C3 serum. The immunization procedure was based uponthe fact that living BCG was first inoculated for the purpose of activa-ting the immune system. After the initial activation, 2 injections of C3-inulin complex with complete Freund adjuvant were made subcutaneously,intramuscularly and intra-glandularly on multiple sites of the rabbit.By means of this method, we found that serum titer was much higher,time interval much shortened, and testing material much saved.

    本文报告利用菊糖从正常人血清中吸附补体C_3,再将菊糖C_3复合物免疫家兔,制备免抗人C_3血清。免疫方法是用活卡介苗代替死卡介苗接种家兔,作为基础免疫,活化免疫系统,然后用微量菊糖C_3复合物加弗氏完全佐剂进行皮下、肌肉与淋巴结内多点直接注射。只免疫两次,即获得较高效价的抗C_3血清。既缩短了免疫过程,也节约抗原用量。

    Mobilization of pluripotent hematopoietic stem cells (CFU-S) into circulation by Ch violaceum endotoxin, and the relationship between sensitivity to endotoxin and the mobilization were studied in mice.It was observed that the circulating CFU-S per 106 MNC had a 2-fold increase on the 5th day after endotoxin.The mobilization of progenitor cells (CFU-GM) by endotoxin was more evident in ICR mice, whichwere more sensitive to endotoxin, than in Kunmin mice.Endotoxin could mobilize more CFU-GM in mice whose sensitivity...

    Mobilization of pluripotent hematopoietic stem cells (CFU-S) into circulation by Ch violaceum endotoxin, and the relationship between sensitivity to endotoxin and the mobilization were studied in mice.It was observed that the circulating CFU-S per 106 MNC had a 2-fold increase on the 5th day after endotoxin.The mobilization of progenitor cells (CFU-GM) by endotoxin was more evident in ICR mice, whichwere more sensitive to endotoxin, than in Kunmin mice.Endotoxin could mobilize more CFU-GM in mice whose sensitivity to endotoxin had been elevated by pretreat-ment with BCG.On the basis of these results, we conclude that the BCG-pretreatment may be an effective measure to raise the yield of CFU-GM.

    本文报告了低毒性的紫色杆菌内毒素对小鼠造血干细胞的动员作用,并研究了动物对内毒索的敏感性与动员作用的关系。结果表明,静脉注射内毒素后,小鼠血中造血干细胞数(CFU-S)逐渐升高,第5天达高峰,为对照组的2倍。在内毒素敏感动物(ICR纯种小鼠),内毒素对粒-巨系祖细胞(CFU-GM)的动员作用明显增强。因此,预先先注射卡介苗,使机体对内毒素的敏感性提高后,内毒素可动员出更多的造血祖细胞。

    This paper reports our investigation of the possibility of the ultiliza- tion of the Bioluminesceht (BL) technique to substitute the traditional viability count of CFU of current BCG vaccine. First of all we established the basic method of BL technique. Secondly we extracted BCG-ATP with ultrasomic-chloro form method, and got the satisfactory resulte. Our results showed: 1. there was significant dose-dependent-correlation between the concentration of standard ATP and ATP BL value; 2. The correlation coefficient...

    This paper reports our investigation of the possibility of the ultiliza- tion of the Bioluminesceht (BL) technique to substitute the traditional viability count of CFU of current BCG vaccine. First of all we established the basic method of BL technique. Secondly we extracted BCG-ATP with ultrasomic-chloro form method, and got the satisfactory resulte. Our results showed: 1. there was significant dose-dependent-correlation between the concentration of standard ATP and ATP BL value; 2. The correlation coefficient values of BL and CFU of liquid and freezedried vaccine Vere found to be 0.8155 and 0.8484 respectively (p<0.05); 3. The coefficients of varialion (CV) between the BL value of ATP obtained by different lots was 3.2~4.4%, this value is much lower than of CFU (CV=10.4~ 11.2%); 4. The presence of bacterial clumps Within the vaccine has showed to have great influence on the ATP BL values and the CFU (Viabi- lity) count. According to the results, we consider that the BL method can be subs- titute for CFU method, and the BL method has not only showed high sen- sitivity, reproducibility and simplicity but also with high accuracy.

    报道用生物发光技术(BL法)取代现行卡介苗菌落形成单位计数法(CFU)的实验研究。建立了标准化BL检测方法:并成功地设计了抽提效果良好的超声-氯仿卡介苗ATP提取法。ATP标准品含量与ATPBL值具有明显剂量依赖关系;卡菌苗菌液和冻干卡介苗菌液的BL值与各相应菌液的CFU数密切相关,相关系数分别为0.8155和0.8484(p<0.05);卡介苗ATPBL值批间变异系数(3.2~4.4%)明显低于CFU数的批间变异系数(10.4~11.2%)。

     
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