(2) Using orthogonal experimental design with orthogonal layout of L16(45), effects of cadmium(0,100,1000,2000μmol/L) and estrous cycle(proestrous,estrous, postestrous, dioestrous)on steroidogenesis in the ovary were studied.
(7) In whole ovary culture, effects of cadmium on estradiol secretion were significantly(P<0.05),and effects of cadmium on progesterone secretion were not significantly (P>0.05). Effects of estrous cycle on estradiol and progesterone secretion in whole ovary culture were significantly (P<0.001,P<0.05).
At the30th day after treatment,the number of normal follicles revealed no significant changes,the number ofⅡatretic follicles in the25and50L/kg group was still significantly higher than that in the control group,the number of corpora lutea in ovary significantly decreased with the in-creases of doses. The estrous cycle delayed at the level of50L/kg.
Methods Two factorial experiments were designed to explore the influence of four dosage levels of cadmium(0,100,(1 000),(2 000) μmol/L) and four different stages(proestrous,estrous,postestrous,dioestrous) of estrous cycle on steroidogenesis in the ovary.
The observation of the ovary Zn and Cu content, estrus cycle and ovary histology were conducted on female rats subchronicly exposed to carbon disulfide(CS2) .
To compare the pregnancy percentage after giving pills with before giving pills,to compare follicle stimulating hormone (FSH)、luteinizing hormone(LH)、estradiol(E 2)、progesterone (P) of each stage estrus cycle,and doing analomical examination.
(2) The results in vitro,showed that the influence of cadmium and the stages of estrus cycle on the contents of E_2 and P of ovary were sigificant and the interaction between them were existing.
[Results]① The period and interval of estrus cycle of experiment groups were longer than normal group,and the normal ratio of estrus cycle was decreased (P＜0.05).
每天以1 ml/ kg 等容量皮下注射,每周染毒5天,持续6周。 [结果]①各染毒组与对照组相比,动情周期、动情间期的延长,与对照组比较均有显著性(P＜0．05)；
Proestrus and metestrus prolonged significantly in 1.91mg/kg and 31.80mg/kg group than that of the control and the duration of estrus and diestrus was reduced.
Results The female's estrous cyclicity became longer compared to the control group and the number of offspring per cote decreased with the increase of the dose.
Kunming adult female mice were exposed to CS2 at concentrations of O, 10 and 100mg/m3 for 2 hours/day, 6 days/week for 3 weeks ( about 3-4 estrous Cycles) . After exposure, the mice were injected intraperitoneally with PMSG and HCG and then were mated with anexposed male mice.
Results:After giving sterilization pill,the sterilization rate was 87.5%,the per embrgo was significantly decreased,FSH there were signal decreased but E 2 and P there were no significantly difference in each stage of estrus.
The GnRH-stimulating test were carried in rats,with the serum FSH,LH,P and E\-2 determined before the injection of GnRH and the serum FSH,LH determined after the injection of GnRH finally the super-ovulation test was done.
To test if leptin responsiveness may be regulated we assayed hypothalamic response to leptin during the estrous cycle; when changes in food intake are known to occur.
To fill this gap in our knowledge, we identified and characterized the tissue specific expression of IGFBP-1 to-6 in rat oviducts over the estrous cycle byin situ hybridization and immunocytochemistry.
Results are tentatively explained, suggesting that the variability of the female response to stress during the different phases of the estrus cycle, permitted them to perform differently after chronic cannabis treatment.
Kunming adult female mice were exposed to CS2 at concentrations of O, 10 and 100mg/m3 for 2 hours/day, 6 days/week for 3 weeks ( about 3-4 estrous Cycles) . After exposure, the mice were injected intraperitoneally with PMSG and HCG and then were mated with anexposed male mice. After mating, mice were sacrificed. The fertilized ova were examined and chromosomal specimens were prepared. The results indicated that the incidence of maldevelopmental ova of the two exposed groups was higher than that of the contr...
The cervical extensibility and dilatation of the isolated uterine cervix of guinea pigwas measured in vitro under isometric conditions: successive extensions of 0.2mm wereperformed at 1-min interval and the resultant tension of the cervix was recorded. On day 8 of estrus cycle, the cervix was rigid, the extensibility was strong and theP level was high: while on day 16, the contrary results were obtained. There was a po-sitive correlationship between the cervical extensibility and the plasma P levels. In pre...
The results showed that:the estrus cycle of group at dosage of 900 ppm. lengthened;incidence of pregnant decreased. Incidence of living embryo and incidence of absorption embryo of each group had no significant difference in comparison with negative control group. F1 generation was observed an increase in incidence of dead embryo ,and a decrease in incidence of living embryo. Offspring's weight at 4th, 21th day in dosage of 900 ppm. significantly lower than that of the negative control group,offspring's beh...
动情周期
estrous cycle
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