Objective:To investigate the relationship between renal tubular epithelial-myofibroblast transdifferentiation (TEMT) and renal interstitial fibrosis in the rats with chronic aristolochic acid nephropathy (CAAN).
Results Compared with the control group, ILK, α-SMA and Vimentin in renal tubular epithelial cells in Group diabetes gradually increased in immunohistochemistry (P<0.01); ILK was consistent with the pathological variation of renal interstitium and was positively correlated to α-SMA(rs=0.621,P<0.05).
Some collagen Ⅲ expression was found in SHAM group,which was much enhanced in UUO group at days 7,14,21 and mainly distributed in renal interstitium,the expression in FTY720 group was also increased compared to SHAM group,but much lower than that in UUO group.
Objective To elucidate the association of renal tubulointerstitial expression of TSP-1(thrombospondin-1) and VEGF(vascular endothelial growth factor) with renal disease in the patients with chronic nephropathy and to evaluate the role of TSP-1 and VEGF in the genesis of chronic renal interstitial fibrosis.
Incontrast, there was high HSP47 expression in the tubular epithelial cells, renal interstitial tissue, glomerular epithelial and mesangial cells of trial groups. The intensity of HSP47 expression was positively correlated with the degree of glomerulosclerosis and tubulointerstitial injury(P<0.01).
Expression of connective tissue growth factor in renal tubulointerstitial fibrosis in rats and its pathogenic role
In order to explore the role of connective tissue growth factor (CTGF) in the pathogenesis of renal tubulointerstitial fibrosis, 48 Wistar rats were randomly divided into sham-operated and unilateral ureteral obstruction (UUO) group.
The renal tubulointerstitial injury index was evaluated according to the MASSON staining.
On the post-UUO day 7, the protein level of CTGF was positively related to the renal tubulointerstitial injury index (r=0.62,P>amp;lt;0.01), the expression of TGF-β1 (r=0.85,P>amp;lt;0.01), col I (r=0.78,P>amp;lt;0.01), and PAI-1 (r=0.76,P>amp;lt;0.01).
The role of protease activated receptor-2 (PAR-2) in the renal tubulointerstitial lesion induced by unilateral ureteral obstruction (UUO) was explored.