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病毒糖蛋白
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  virus glycoprotein
     After IPTG induction,ELISA results showed that both fusion proteins could bind specifically to the hantavirus nucleoprotein specific mAb, and the fusion protein GSTG1S0.7 could bind Hantaan virus glycoprotein specific mAb.
     经IPTG诱导后,ELISA活性测定结果表明,两种融合蛋白均可与抗汉坦病毒NP的mAb特异性结合,融合蛋白GST-G1S0.7还可与抗汉滩病毒糖蛋白的mAb特异性结合。
短句来源
     Expression and purification of varicella-zoster virus glycoprotein I gene in insect cells
     水痘-带状疱疹病毒糖蛋白I基因在昆虫细胞中的表达及纯化
短句来源
     Construction of prokaryotic expression vector of varicella-zoster virus glycoprotein E and the purification of its expressed product
     水痘-带状疱疹病毒糖蛋白E原核表达载体的表达及产物纯化
短句来源
     The recombinant adenovirus carrying Hantaan virus glycoprotein G 2 gene was obtained, whose titer was 10 10 pfu/mL, and Hantaan virus glycoprotein G 2 was detected in infected HEK293 cells.
     得到了含汉滩病毒G2 基因的重组腺病毒 ,其滴度约为 1 0 10 pfu/mL ,同时在感染的HEK2 93细胞中检测到汉滩病毒糖蛋白G2 的表达。
短句来源
     Construction of the eukaryotic expression vector containing gene of varicella-zoster virus glycoprotein E
     水痘-带状疱疹病毒糖蛋白E表达载体的构建
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  virus glycoprotein cdna
     Study on Construction of Transgenic Mice Carrying Ra-bies Virus Glycoprotein cDNA
     狂犬病病毒糖蛋白转基因小鼠的构建研究
短句来源
     Effects of Different Transfection Reagents on Genetic Immunization of Rabies Virus Glycoprotein cDNA
     不同转染介质对狂犬病病毒糖蛋白基因免疫的效应
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  “病毒糖蛋白”译为未确定词的双语例句
     Functional Analysis of Glycoproteins of Newcastle Disease Virus F 48 E 9 Strain
     新城疫病毒F48E9株病毒糖蛋白的功能分析
短句来源
     Cloning and Expression of the gp50 Gene of Pseudorabies Viurus
     伪狂犬病病毒糖蛋白gp50基因的克隆和表达
短句来源
     Analysis of the Structure and Prokaryotic Expressing of the Glycoprotein G Gene of Pseudorabies Virus
     伪狂犬病病毒糖蛋白G基因的结构分析及其原核表达
短句来源
     Molecular Chaperones and Virus Glycoproteins
     分子伴侣与病毒糖蛋白
短句来源
     To investigate the immunological properties of hantaan virus chimeric gene G2S0.7, Balb/c mice were directly immunized by G2S0.7 recombinant adenovirus.
     对汉滩病毒糖蛋白 (GP)和核蛋白 (NP)的嵌合基因G2S0 .7重组腺病毒的免疫学特性进行研究。
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  virus glycoprotein
Immunochemical Properties of Recombinant Polypeptides Mimicking Domains I and II of West Nile Virus Glycoprotein E
      
Expression and antigenic characterization of the epitope-G1 of the Bovine ephemeral fever virus glycoprotein in Pichia pastoris
      
The efficiency was examined of immunization with feline leukemia virus glycoprotein complexes (gp85 rosettes) to protect cats against tumors induced by feline sarcoma virus (FeSV).
      
A peptide-model for the heparin-binding property of pseudorabies virus glycoprotein III
      
The pseudorabies virus glycoprotein III (PrV-gIII) has been identified previously as the major viral component binding to a heparin-like receptor on the surface of target cells.
      
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The three structural proteins of sindbis virus were examined,using the methodof peptide mapping of ~(125)I-labeled proteins in single polyacrylamide gel slices.Two-dimensional tryptic peptide maps of E_1 and E_2 are similar,suggesting that they areprobably related.There are differences between these two polypeptides,each of whichgives oligopeptides lacking in the other.But there are no fingerprint similaritiesbetween protein C and either of the two envelope glycoproteins,suggesting that theyare unrelated.The...

The three structural proteins of sindbis virus were examined,using the methodof peptide mapping of ~(125)I-labeled proteins in single polyacrylamide gel slices.Two-dimensional tryptic peptide maps of E_1 and E_2 are similar,suggesting that they areprobably related.There are differences between these two polypeptides,each of whichgives oligopeptides lacking in the other.But there are no fingerprint similaritiesbetween protein C and either of the two envelope glycoproteins,suggesting that theyare unrelated.The same pictures can be obtained even when tryptio digests of the threeproteins were put together in a mixture.The results suggested that the three structuralproteins of sindbis virus have different amino acid sequences.

采用双向肽图法对Sindbis病毒糖蛋白(E_1和E_2)及衣壳蛋白(C)的寡肽进行了分析。首先,用不连续的SDS-聚丙烯酰胺凝胶电泳对该病毒的三种结构蛋白进行分离,经考马斯蓝染色定位后,分别切取蛋白带。然后,按改良的氯氨T法用~(125)I直接标记胶条中的蛋白质;~(125)I标记的蛋白经TPCK-胰酶消化后,在硅胶板上进行电泳和层析。结果表明,三种结构蛋白的肽图是不同的。但两种糖蛋白分子E_1和E_2具有某些类似之处,因此,它们可能含有一些共同的顺序;与衣壳蛋白C相比则截然不同。说明Sindbis病毒的结构蛋白E_1、E_2和C具有不同的氨基酸排列顺序。

A series of research work has been completed in recent years in this labor- atory with monoclonal antibodies (McAb) against herpes simplex virus (HSV). (1) A novel HSV-2 glycoprotein (g30K) was identified and the gene coding for it was localized to the unique long (UL) region at map units 0.490 to 0.564. The target antigens of 7 McAbs (gC and gD) were also characterized. (2) Two double sandwich McAb-ELISA tests were developed to detect and type HSV antigen and antibody in more than 1000 clinical specimens. (3)...

A series of research work has been completed in recent years in this labor- atory with monoclonal antibodies (McAb) against herpes simplex virus (HSV). (1) A novel HSV-2 glycoprotein (g30K) was identified and the gene coding for it was localized to the unique long (UL) region at map units 0.490 to 0.564. The target antigens of 7 McAbs (gC and gD) were also characterized. (2) Two double sandwich McAb-ELISA tests were developed to detect and type HSV antigen and antibody in more than 1000 clinical specimens. (3) The rabbits with experimental herpes simplex keratitis (HSK) by infection with HSV were treated with McAbs to HSV gC and gD. All the rabbits in McAb treat group were cured. The mechanisms of treatment are involved in neutralization and ADCC. (4) 204 patients with HSK, herpetic cervicitis, herpetic vulvitis and herpetic stomatitis received anti-HSV McAb therapy. The effect rates were 100%, 92.6%, 100% and 95.5%, respectively. The evidences suggest that McAbs may be effective agents to treat HSV infection.

应用本室制备的8株抗单纯疱疹病毒糖蛋白单克隆抗体(抗HSV McAb)进行了系列研究,①鉴定出一种新的HSV糖蛋白g30K、gC的一种新形式,以及gC和gD;②建立了HSV感染的临床标本作抗原抗体分型检测的McAb-ELISA双夹心法,检测961份标本效果良好,并己在全国各地一些医疗单位推广使用;③证明McAb治疗家兔实验性单纯疱疹性角膜炎(HSK)有显著效果,并探讨其治疗机理主要为中和作用和ADCC效应;④对部分志愿者用McAb治疗单纯疱疹性角膜炎、妇女生殖器疱疹和小儿口腔疱疹性糜烂,取得明显疗效。

Two HFRS virus glycoproteins with molecular weight of 78K and 57K were purified by immunoaffinity chromatography with preparative SDS-PAGE from virus-infected cells Purified 78K and 57K glycoproteins all showed only one band on SDS-PAGE and have antigenieity and immunogenicity, The results of hemagglutination test indicate that 57K glyeoprotein has hemagglutation activity. Neutralizing test with antisera confirm both 78K and 57K glyeoproteins have neutralizing antigenic determinants. These results suggest that...

Two HFRS virus glycoproteins with molecular weight of 78K and 57K were purified by immunoaffinity chromatography with preparative SDS-PAGE from virus-infected cells Purified 78K and 57K glycoproteins all showed only one band on SDS-PAGE and have antigenieity and immunogenicity, The results of hemagglutination test indicate that 57K glyeoprotein has hemagglutation activity. Neutralizing test with antisera confirm both 78K and 57K glyeoproteins have neutralizing antigenic determinants. These results suggest that these two glycoproteins are analogous to G1 and G2 glycoproteins of virion of HFRS virus.

以感染肾综合征出血热病毒(HFRSV)的Vero E6细胞为材料,用免疫亲和层析结合制备聚丙烯酰胺凝胶电泳(PAGE)从感染细胞中提纯了HFRSV两种糖蛋白。先用免疫亲和层析从感染细胞的粗制抗原中获得含有四种蛋白的混合液,用[~3H]-氨基葡萄糖在感染细胞中标记病毒糖蛋白,观察到[~3H]-氨基葡萄糖只结合入78K和57K的病毒蛋白。再用制备SDS-PAGE从HFRSV混合液中提纯78K和57K两种蛋白。实验证明这两种糖蛋白均具中和抗原决定簇,57K的糖蛋白尚具血凝活性,初步鉴定表明这两种糖蛋白相当于文献报道的HFRSV G_1和G_2。

 
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