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植酸酶
相关语句
  phytase
    Cloning and Expression of Phytase phyC Gene from Bacillus Subtilis
    枯草芽孢杆菌植酸酶phyC基因的克隆及其表达研究
短句来源
    Study of Phytase Gene Being Cloning and Maize Being Transformed
    植酸酶基因的克隆及转化玉米的研究
短句来源
    Results showed that expression efficiency of phytase gene reached its highest expression when the amountof vitamin C takes up the total dry diet weight 1%,that of vitamin B compound is 0.25%,vitaminB6 is 2mg/100g dry matter weight and inorganic salt is1%.
    人工饲料中VC添加量为饲料干物重的1.0%、VB 混合物为0.25%、VB6为2mg/100g干物、无机盐添加量为饲料干物重的1.0%时,植酸酶基因表达活性最高。
    Expression and Regulation of a Phytase Gene (Ⅰ)——Phytase Activity and Mobilization of Phosphorus Storage in Germination
    植酸酶基因表达与调控机制研究(Ⅰ)——发芽过程中植酸酶动态与贮藏态磷动员
短句来源
    Studies on Asp.ficuum NRRL3135 Phytase Molecular Biology
    Asp·ficuum NRRL3135植酸酶分子生物学研究
短句来源
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  recombinant phytase
    Research Progress in Transgenic Plants Expressing Recombinant Phytase
    转基因植物表达植酸酶研究进展
短句来源
    Identification and Characterazation of Methyl-parathion Degrading Bacteria & Purification and Characterazation of Recombinant Phytase PHYA
    甲基对硫磷降解菌的鉴定与降解性能分析和植酸酶PHYA的纯化与酶学性质研究
短句来源
    Studies on the partial enzyme characters of recombinant phytase showed that the recombinant phytase had two pH optima (2.5-3.0 and 5.0-5.5) and a temperature optimum 55℃, and with higher activity under the pH ranged from 4.5 to 6.0. The result of SDS-PAGE indicated that the molecular weight of the recombinant phytase is between 70kDa-97kDa.
    重组植酸酶部分酶学性质的研究结果表明:重组酶在pH值2.5一3.0和5.0一5.5时酶活性最高,且在pH4.5一6.5之间均有较高的酶活性,最适作用温度为55℃。 sDs一PAGE的结果显示重组植酸酶的分子量介于70kDa~97kDa之间。
短句来源
    Specific activity of the rude recombinant phytase is 461575.8 U/mg.
    重组植酸酶粗酶比活力为461575.8 u/mg。
短句来源
    crassa phy was also cloned as an EcoR I fragment into the corresponding site of the expression vector pPIC9K downstream of the AOX1 promoter. The resulting plasmid pPIC9K-phy was linearized with Nco I endoenzyme and subsequently electrotransformed into P. pastoris KM71. The recombinant phytase was purified as follows.
    crassa植酸酶基因插入到带有酵母信号肽α因子的表达载体pPIC9K的EcoR I位点,正向连接的重组质粒pPIC9K-phy 经Nco I酶切纯化后,电转化入巴斯德毕赤氏酵母KM71。
短句来源
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  “植酸酶”译为未确定词的双语例句
    The PhyB1 of A. niger WP1 strain in this paper its sequence has been accessed by GeneBank(Accession:DQ836360).
    黑曲霉WP1植酸酶PhyB1基因序列已在国际基因库中注册,注册号为:DQ836360。
短句来源
    This experiment studied the effects of vitamin and inorganic salt in artificial diet onthe foreign gene expression efficiency of rBmNPV—Bm expression system by changing theaddictive amount of vitamin C(VC),vitamin B(VB),vitamin B_6(VB_6)and inorganic salt.
    以植酸酶基因为报告基因,探讨了人工饲料中维生素C(VC)、B 族维生素(VB)混合物、维生素B6(VB6)和无机盐混合物的添加量对rBmNPV-Bm 表达系统外源基因表达活性的影响。
    Advances in the reaseaches on the phyA Gene of Filamentous Fungi
    真菌植酸酶phyA基因研究进展
短句来源
    Studies on high density culture conditions of producing-phytase Pichia pastoris combined strain
    毕赤氏酵母植酸酶工程菌高密度培养条件的研究
短句来源
    MUTAGENIC BREEDING AND ENZYME PRODUCING CONDITIONS OF PHYTASE-PRODUCING STRAIN CN-92
    CN-92植酸酶产生菌的诱变选育及产酶条件的研究
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  phytase
RecombinantPichia pastoris overexpressing bioactive phytase
      
Phytase genephyA2, whose signal peptide encoding sequence and intron sequence had been removed, was modified.
      
The result of SDS-PAGE of the phytase expressed by P.pastoris showed that the modifiedphyA2 had been overexpressed and secreted.
      
The concentration of the phytase expressed by P.pastoris with modifiedphyA2 exceeded 15 000 U/mL, which had a 3 000-fold increase over that of originAspergillus niger 963 and was 37 times higher than that of recombinantP.
      
In this paper, progresses in researches on physiology of Zn deficiency in animals, phytate effect on bioavailability of Zn, and role of phytase in healing Zn deficiency of animals were reviewed.
      
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  recombinant phytase
The recombinant phytase had one optimum pH (pH?4.5) and an optimum temperature of 50°C.
      
Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase.
      
These results suggest a commercial feasibility of producing a stable recombinant phytase in canola at a high level for animal feed supplement and for reducing phosphorus eutrophication problems.
      
Detailed analysis of immature seeds by immunofluorescence and electron microscopy showed that recombinant phytase carrying a signal peptide for entry into the endoplasmic reticulum was efficiently secreted from storage cotyledon cells.
      
The medium for recombinant phytase production by E.
      
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The relation between phytase activity and seed germination is reported in this paper. The remits showed that phytase activity played an important role during seed germination. When phvtase activity was enhanced, the seed could be bud, or it did not germinate even if in the suitable conditions such as water, temperature and so on. The results affor us a useful evi-dence of endogenous factors of seed germination.

报道水稻种子植酸酶活性对种子萌发的影响。实验结果表明,在种子由休眠转入萌发状态的过程中,植酸酶起着重要作用:植酸酶活性增强,种子可以萌发;植酸酶活性被抑制,即使给予合适的外界条件,如水分、温度等,种子也不能萌发。从而为深入探讨种子萌发的内因提供了具有重要参考价值的依据。

A sheet of agar gel(1.5%~2.0%,1mm thickness)on copy film was soaked in Gly-HCl buffer containing 1 .2m mol/L sodium phytate for over 1h and then taken out.After the moisture on thegel surface disappeared, phytase was spotted on or an electrophoresed polyacryamide gel contaillingphytase was placed in contact with it for 10~60min,then the agar gel was horizontally incubated inwater bath for 15 min at 37oC,finally the gel was immersed in lmol/L H2SO4-2%(NH4)6Mo7O24-10% FeSO4 for 2~5min.to develop its colour.This...

A sheet of agar gel(1.5%~2.0%,1mm thickness)on copy film was soaked in Gly-HCl buffer containing 1 .2m mol/L sodium phytate for over 1h and then taken out.After the moisture on thegel surface disappeared, phytase was spotted on or an electrophoresed polyacryamide gel contaillingphytase was placed in contact with it for 10~60min,then the agar gel was horizontally incubated inwater bath for 15 min at 37oC,finally the gel was immersed in lmol/L H2SO4-2%(NH4)6Mo7O24-10% FeSO4 for 2~5min.to develop its colour.This assay is simple, rapid andhighsensitive, as little as 0.0045 μ phytase on one cm2 of gel could be detected,which is suitable forscreening microorganism,zymogram analysis and comparing specific activities of phytases.

将1mm厚凝固于复印膜上的水琼脂(15%~2.0%)凝胶板侵入含12mmol/L植酸钠的Gly—HCl缓冲液中达1h以上,取出干至胶表面无水迹,于上加Aspergillussp.59—2植酸酶或与电泳后的凝胶板紧贴10~60min。然后水平置于恒温水浴锅中反应一定时间,取出浸入1mol/LH2SO4-2%(NH4)6Mn7O24-10%FeSO4溶液显色2~5min。该法简便、快速、灵敏,反应15min即可检出每cm2低于0.0045μ的植酸酶。此法适用于微生物的初筛、酶谱分析及酶比活的比较。

Some strains producing phytase were selected. We have compared the conditions of producing phytase by Asp. ficuum NRRL3135 and Asp. niger 70 in solid state fermentation process.

经采样、分离,筛选出多株植酸酶产生菌;并将其中产酶水平较高的黑曲霉(Asp.niger70)和无花果曲霉(Asp.ficuumNRRL3135)用固态培养法进行了产植酸酶条件的对比研究。

 
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