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植酸酶
相关语句
  phytase
    Studies on the expression of Aspergillus niger phytase gene in Escherichia coli JM-109-pTrcHis2A-phyA
    工程菌JM-109-pTrcHis2A-phyA表达植酸酶的研究
短句来源
    Phytic Acid and Phytase
    植酸与植酸酶
短句来源
    Studies on the fermentation conditions of phytase from Penicillium sp. LQ-7
    青霉LQ-7植酸酶产生条件研究
短句来源
    A Penicillium sp.which can produce phytase with high-activity has been screened and mutated by UV.The best phytase-producing medium is: 3% soluble starch,0.5% peptone,0.5% NH 4NO 3,0.05% MgSO 4·7H 2O,0.05% FeSO 4·7H 2O,0.001% MnSO 4·4H 2O.
    筛选到一株植酸酶高产菌株 ,对其进行诱变并研究了该菌株产植酸酶的最适条件。 优化产酶液体培养基组成为 :3%可溶性淀粉 ,0 5 %蛋白胨 ,0 5 %NH4NO3 ,0 0 5 %MgSO4·7H2 O ,0 0 5 %FeSO4·7H2 O ,0 0 0 1%MnSO4·4H2 O ;
短句来源
    Primary pH of medium at 6.5,inoculum size at 4%(V/V),fermentation for 72 hours are beneficial to the yield of phytase.
    发酵培养基的起始pH为 6 5 ,接种量 4%的条件下 30℃ ,135r min培养 72h可获得较高的植酸酶产量。
短句来源
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  “植酸酶”译为未确定词的双语例句
    Experiments prove that IP_3~IP_6 in sample could rapidly be separated and determined by ion-pair HPLC,IP_2~IP_6 in it is determined by HPIC,which also separated isomers of IP_3~IP_5,and IP_1~IP_3 and their isomers could be seperated by HPIC.
    离子色谱方法1可以有效的分离和鉴定IP2~IP6及IP3~IP5的异构体,离子色谱方法2可以有效测定IP1~IP3及它们的异构体,离子色谱方法1和方法2结合使用可以完全分离植酸酶水解植酸产物中所有的磷酸肌醇及它们的异构体。 研究还表明A.
短句来源
    The application of HPLC in purification and determination of isomers of inositol mono- to hexaphosphates from phytate by extra-cellular phytases
    色谱法在植酸酶水解产物分离和鉴定中的应用
短句来源
    Conclusion: This expression system can be used as a model that will be helpful for mutagenesis experiments.
    结论 :该表达系统被用做研究黑曲霉植酸酶体外改良的工具 .
短句来源
  相似匹配句对
    THERMOSTABLE PHYTASE FROM ASPERGILLUS SP.
    热稳定的曲霉植酸酶
短句来源
    Phytic Acid and Phytase
    植酸与植酸酶
短句来源
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  phytase
RecombinantPichia pastoris overexpressing bioactive phytase
      
Phytase genephyA2, whose signal peptide encoding sequence and intron sequence had been removed, was modified.
      
The result of SDS-PAGE of the phytase expressed by P.pastoris showed that the modifiedphyA2 had been overexpressed and secreted.
      
The concentration of the phytase expressed by P.pastoris with modifiedphyA2 exceeded 15 000 U/mL, which had a 3 000-fold increase over that of originAspergillus niger 963 and was 37 times higher than that of recombinantP.
      
In this paper, progresses in researches on physiology of Zn deficiency in animals, phytate effect on bioavailability of Zn, and role of phytase in healing Zn deficiency of animals were reviewed.
      
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Aim: Phytase improves the bioavalability of phytate phosphorus in plant foods to humans and animals and reduces phosphoruspollution of animal waste. Our projects were to express an Aspergillus niger phytase gene (phyA) in Escherichia coli under the control of the try(trp-lac) promoter. Methods: The recombined gene pTrcHis2A-phyA was constructed by methods of gene engineering and the expression of phytase gene was induced by isopropyl-β-thiogalactopyranoside (IPTG). Results: The refolded enzyme had an activity...

Aim: Phytase improves the bioavalability of phytate phosphorus in plant foods to humans and animals and reduces phosphoruspollution of animal waste. Our projects were to express an Aspergillus niger phytase gene (phyA) in Escherichia coli under the control of the try(trp-lac) promoter. Methods: The recombined gene pTrcHis2A-phyA was constructed by methods of gene engineering and the expression of phytase gene was induced by isopropyl-β-thiogalactopyranoside (IPTG). Results: The refolded enzyme had an activity of 830 U/mL medium at 37℃. But part of the protein was in the form of inactive aggregates. Conclusion: This expression system can be used as a model that will be helpful for mutagenesis experiments.

目的 :将来源于黑曲霉的植酸酶基因重组于大肠杆菌表达载体pTrcHis2A中 ,导入大肠杆菌JM10 9中 ,构建工程菌JM - 10 9-pTrcHis2A -phyA .方法 :基因重组 ,以及克隆和外源基因诱导表达技术 .结果 :该工程菌能够有效地表达真核植酸酶基因 .并且添加诱导剂IPTG ,能使植酸酶的表达量成倍提高 .结论 :该表达系统被用做研究黑曲霉植酸酶体外改良的工具 .

Phytic acid has the positive and negative effects on human beings, domestic animals and the natural environment. These two effects are the results of its function of chelation and antioxidant. It is suggested to produce phytic acid by microbial fermentation method. The later part of this article puts forward the development and utilizing phytase to eliminate antinutritive effect of phytic acid in food and feed which will bring us great economic and social benefits.

植酸对生态环境有正负两方面的效应 ,皆是其螯合作用和抗氧化作用的结果。文中提出用微生物发酵法生产植酸的设想及其依据 ,建议开发和利用植酸酶用以消除食品和饲料中植酸的抗营养作用 ,以及预示可能会带来的巨大经济和社会效益

Phytase is a new and important enzyme used in animal feed and food additive. It can decompose phytic acid to increase the utilization rate of phosphorus and to decrease the environmental pollution from phosphorus. Meanwhile it can prompt the absorption of calcium, magnesium, and copper. The progress and trends related to application and research of phytase in recent studies is reviewed.

植酸酶是一种新型的 ,可作为饲料及食品添加剂的重要酶制剂。它能将食品和饲料中植酸及其盐转化为可供有机体利用的有效磷 ,降低粪便中的磷含量 ,减轻对环境的污染 ,消除植酸对金属离子的螯合作用 ,改善营养成分的吸收和利用。本文综述了国内外的研究与应用现状及其进一步的发展方向

 
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