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植酸酶
相关语句
  phytase
    Studies on the expression of Aspergillus niger phytase gene in Escherichia coli JM-109-pTrcHis2A-phyA
    工程菌JM-109-pTrcHis2A-phyA表达植酸酶的研究
短句来源
    Phytic Acid and Phytase
    植酸与植酸酶
短句来源
    Studies on the fermentation conditions of phytase from Penicillium sp. LQ-7
    青霉LQ-7植酸酶产生条件研究
短句来源
    Prospect of the phytase application in food and medicine
    植酸酶在食品和医药方面的应用展望
短句来源
    A Penicillium sp.which can produce phytase with high-activity has been screened and mutated by UV.The best phytase-producing medium is: 3% soluble starch,0.5% peptone,0.5% NH 4NO 3,0.05% MgSO 4·7H 2O,0.05% FeSO 4·7H 2O,0.001% MnSO 4·4H 2O.
    筛选到一株植酸酶高产菌株 ,对其进行诱变并研究了该菌株产植酸酶的最适条件。 优化产酶液体培养基组成为 :3%可溶性淀粉 ,0 5 %蛋白胨 ,0 5 %NH4NO3 ,0 0 5 %MgSO4·7H2 O ,0 0 5 %FeSO4·7H2 O ,0 0 0 1%MnSO4·4H2 O ;
短句来源
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  “植酸酶”译为未确定词的双语例句
    K~+? Al~(3+)? Zn~(2+),and the activity is determined by vitamin c-ammonium phosphomolybdate in blue spetrophotometric method,Mg~(2+) and Ca~(2+) are selected as the proper activators.
    微生物植酸酶法提取肌醇时,利用维C-磷钼蓝吸光光度法,测定部分常见阳离子激活剂M g2+、C a2+、M n2+、K+、A l3+、Zn2+对其酶活的影响,从中筛选出合适的植酸酶激活剂Zn2+、K+。
短句来源
    Conclusion: This expression system can be used as a model that will be helpful for mutagenesis experiments.
    结论 :该表达系统被用做研究黑曲霉植酸酶体外改良的工具 .
短句来源
  相似匹配句对
    THERMOSTABLE PHYTASE FROM ASPERGILLUS SP.
    热稳定的曲霉植酸酶
短句来源
    Phytic Acid and Phytase
    植酸与植酸酶
短句来源
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  phytase
RecombinantPichia pastoris overexpressing bioactive phytase
      
Phytase genephyA2, whose signal peptide encoding sequence and intron sequence had been removed, was modified.
      
The result of SDS-PAGE of the phytase expressed by P.pastoris showed that the modifiedphyA2 had been overexpressed and secreted.
      
The concentration of the phytase expressed by P.pastoris with modifiedphyA2 exceeded 15 000 U/mL, which had a 3 000-fold increase over that of originAspergillus niger 963 and was 37 times higher than that of recombinantP.
      
In this paper, progresses in researches on physiology of Zn deficiency in animals, phytate effect on bioavailability of Zn, and role of phytase in healing Zn deficiency of animals were reviewed.
      
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Inositol can be obtained from phytin by hydrolysis of two kinds of enzyme.At first,phytase hydrolyze phytic acid and phytin into phosphatidypylinositol,and then the production can farther be hydrolyzed into inositol by phosphatase.The two kinds of enzyme can be produced by Aspergillus ficuum at the same time.The optimum medium ingredient,seed age,ventilation volume and initial pH value were determined.When A.ficuum was cultured in the optimum condition,phytase...

Inositol can be obtained from phytin by hydrolysis of two kinds of enzyme.At first,phytase hydrolyze phytic acid and phytin into phosphatidypylinositol,and then the production can farther be hydrolyzed into inositol by phosphatase.The two kinds of enzyme can be produced by Aspergillus ficuum at the same time.The optimum medium ingredient,seed age,ventilation volume and initial pH value were determined.When A.ficuum was cultured in the optimum condition,phytase and phosphatase concentration reached 5.7 u/mL and 0.533 u/mL respectively.The hydrolyzing yield of inositol from phytin was 10.3% by using fermentation solution of A.ficuum.

植酸酶能将植酸及其二价盐类菲丁水解生成磷脂酰肌醇,在磷酸酯酶的继续作用下,磷脂酰肌醇被分解为肌醇。无花果曲霉能同时分泌植酸酶和磷酸酯酶。通过对无花果曲霉(A.ficuumA.s3.324)产生植酸酶和磷酸酯酶条件的研究,在确定的最佳条件下,植酸酶和磷酸酯酶活力分别达到5.7u/mL、0.533u/mL。用所得发酵液水解菲丁制备肌醇,使肌醇的转化率达10.3%。

Aim: Phytase improves the bioavalability of phytate phosphorus in plant foods to humans and animals and reduces phosphoruspollution of animal waste. Our projects were to express an Aspergillus niger phytase gene (phyA) in Escherichia coli under the control of the try(trp-lac) promoter. Methods: The recombined gene pTrcHis2A-phyA was constructed by methods of gene engineering and the expression of phytase gene was induced by isopropyl-β-thiogalactopyranoside (IPTG). Results: The refolded enzyme had an activity...

Aim: Phytase improves the bioavalability of phytate phosphorus in plant foods to humans and animals and reduces phosphoruspollution of animal waste. Our projects were to express an Aspergillus niger phytase gene (phyA) in Escherichia coli under the control of the try(trp-lac) promoter. Methods: The recombined gene pTrcHis2A-phyA was constructed by methods of gene engineering and the expression of phytase gene was induced by isopropyl-β-thiogalactopyranoside (IPTG). Results: The refolded enzyme had an activity of 830 U/mL medium at 37℃. But part of the protein was in the form of inactive aggregates. Conclusion: This expression system can be used as a model that will be helpful for mutagenesis experiments.

目的 :将来源于黑曲霉的植酸酶基因重组于大肠杆菌表达载体pTrcHis2A中 ,导入大肠杆菌JM10 9中 ,构建工程菌JM - 10 9-pTrcHis2A -phyA .方法 :基因重组 ,以及克隆和外源基因诱导表达技术 .结果 :该工程菌能够有效地表达真核植酸酶基因 .并且添加诱导剂IPTG ,能使植酸酶的表达量成倍提高 .结论 :该表达系统被用做研究黑曲霉植酸酶体外改良的工具 .

Phytic acid has the positive and negative effects on human beings, domestic animals and the natural environment. These two effects are the results of its function of chelation and antioxidant. It is suggested to produce phytic acid by microbial fermentation method. The later part of this article puts forward the development and utilizing phytase to eliminate antinutritive effect of phytic acid in food and feed which will bring us great economic and social benefits.

植酸对生态环境有正负两方面的效应 ,皆是其螯合作用和抗氧化作用的结果。文中提出用微生物发酵法生产植酸的设想及其依据 ,建议开发和利用植酸酶用以消除食品和饲料中植酸的抗营养作用 ,以及预示可能会带来的巨大经济和社会效益

 
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