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植酸酶
相关语句
  phytase
    In this study the phytase gene BAP from Bacillus amyloliquefaciens was cloned and fused in the expression plasmid pET-30a(+). then transferred into the host E.
    本研究克隆了淀粉液化芽孢杆菌植酸酶基因,采用表达载体pET-30a(+)Vector、pPIC9K Vector及其相应的宿主菌E.
短句来源
    Its genome was used as template for gene cloning, the phytase gene of BA was amplified by the touchdown polymerase chain reaction (TD-PCR) with primers (5BAP01 and 3BAP01) designed according to the conserved regions of the phytase gene in Genbank.
    通过对植酸酶基因序列的同源性比较,根据其保守区域设计上游引物5BAP01和下游引物3BAP01。 以提取的淀粉液化芽孢杆菌BA基因组为模板,利用TD-PCR技术克隆了淀粉液化芽孢杆菌植酸酶基因BAP,并构建了重组质粒pGEM-T Easy BAP Vector。
短句来源
    STUDIES ON THE INDUCED MUTATION OF A PENICILLIUM STRAIN PRODUCING PHYTASE
    产植酸酶青霉菌株的诱变研究
短句来源
    Purification and Characterization of Phytase Induced in Tomato Roots under PhosphorusDeficient Conditions
    番茄植酸酶分离提纯及其特性研究
短句来源
    Overexpression of Phytase Gene From B.amyloliquefaciens in Escherichia coli and Pichia Pastoris
    淀粉液化芽孢杆菌中性植酸酶基因在大肠杆菌和毕赤酵母中的高效表达
短句来源
更多       
  phytase
    In this study the phytase gene BAP from Bacillus amyloliquefaciens was cloned and fused in the expression plasmid pET-30a(+). then transferred into the host E.
    本研究克隆了淀粉液化芽孢杆菌植酸酶基因,采用表达载体pET-30a(+)Vector、pPIC9K Vector及其相应的宿主菌E.
短句来源
    Its genome was used as template for gene cloning, the phytase gene of BA was amplified by the touchdown polymerase chain reaction (TD-PCR) with primers (5BAP01 and 3BAP01) designed according to the conserved regions of the phytase gene in Genbank.
    通过对植酸酶基因序列的同源性比较,根据其保守区域设计上游引物5BAP01和下游引物3BAP01。 以提取的淀粉液化芽孢杆菌BA基因组为模板,利用TD-PCR技术克隆了淀粉液化芽孢杆菌植酸酶基因BAP,并构建了重组质粒pGEM-T Easy BAP Vector。
短句来源
    STUDIES ON THE INDUCED MUTATION OF A PENICILLIUM STRAIN PRODUCING PHYTASE
    产植酸酶青霉菌株的诱变研究
短句来源
    Purification and Characterization of Phytase Induced in Tomato Roots under PhosphorusDeficient Conditions
    番茄植酸酶分离提纯及其特性研究
短句来源
    Overexpression of Phytase Gene From B.amyloliquefaciens in Escherichia coli and Pichia Pastoris
    淀粉液化芽孢杆菌中性植酸酶基因在大肠杆菌和毕赤酵母中的高效表达
短句来源
更多       
  phytase
    In this study the phytase gene BAP from Bacillus amyloliquefaciens was cloned and fused in the expression plasmid pET-30a(+). then transferred into the host E.
    本研究克隆了淀粉液化芽孢杆菌植酸酶基因,采用表达载体pET-30a(+)Vector、pPIC9K Vector及其相应的宿主菌E.
短句来源
    Its genome was used as template for gene cloning, the phytase gene of BA was amplified by the touchdown polymerase chain reaction (TD-PCR) with primers (5BAP01 and 3BAP01) designed according to the conserved regions of the phytase gene in Genbank.
    通过对植酸酶基因序列的同源性比较,根据其保守区域设计上游引物5BAP01和下游引物3BAP01。 以提取的淀粉液化芽孢杆菌BA基因组为模板,利用TD-PCR技术克隆了淀粉液化芽孢杆菌植酸酶基因BAP,并构建了重组质粒pGEM-T Easy BAP Vector。
短句来源
    STUDIES ON THE INDUCED MUTATION OF A PENICILLIUM STRAIN PRODUCING PHYTASE
    产植酸酶青霉菌株的诱变研究
短句来源
    Purification and Characterization of Phytase Induced in Tomato Roots under PhosphorusDeficient Conditions
    番茄植酸酶分离提纯及其特性研究
短句来源
    Overexpression of Phytase Gene From B.amyloliquefaciens in Escherichia coli and Pichia Pastoris
    淀粉液化芽孢杆菌中性植酸酶基因在大肠杆菌和毕赤酵母中的高效表达
短句来源
更多       
  “植酸酶”译为未确定词的双语例句
    Based on two primers 5BAP01 containing a BamHI site and 3BAP01 containing a Xhol site, the pGEM? T Easy BAP Vector was double digested with BamHI and Xhol, and then cloned into the expression vector pET-30a(+) previously digested with the same enzymes. The resulting plasmid BAPpET30a(+)Vector was introduced into E.
    通过带限制性内切酶位点的引物(5BAP01带BamHI位点,3BAP01带XhoI位点),将淀粉液化芽孢杆菌编码的中性植酸酶基因定向插入到原核表达载体pET-30a(+)上,得到重组表达质粒BAP-pET-30a(+)Vector。
短句来源
    A pair of expression primers, the sense primer 5BAPK01 containing a SnaBI site and antisense primer 3BAPK01 containing a NotI site were designed according to the Multiple Cloning Sites(MCS) of expression vector pPIC9K and the Open Reading Frame of BA gene. The PCR fragment were cloned into pGEM?
    根据酵母表达载体pPIC9K的多克隆位点和已克隆的中性植酸酶基因限制性内切酶图谱,设计具有SnaBI位点的上游引物5BAPK01和具有NotI位点的下游引物3BAPK01。
短句来源
    Studies on the Culture Conditions of a Strain of Producing-phytase Penicillium sp.
    青霉菌产植酸酶液体发酵条件研究
短句来源
    The effect of the various carbon and nitrogen sources on this strain was studied.
    研究了不同碳源,氮源对青霉菌液体发酵产植酸酶的影响。
短句来源
    The best media obtained from the orthogonal test in this culture conditions are as follows:corn starch 2%,sucrose 1.5%,peptone 1%,ammonia nitrate 0.2% and a small amounts of mineral salts.
    通过正交试验获得适于产植酸酶的液体培养基的配方为:玉米淀粉2%,蔗糖1.5%,蛋白胨1%,硝酸铵0.2%以及少许无机盐。
短句来源
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  phytase
RecombinantPichia pastoris overexpressing bioactive phytase
      
Phytase genephyA2, whose signal peptide encoding sequence and intron sequence had been removed, was modified.
      
The result of SDS-PAGE of the phytase expressed by P.pastoris showed that the modifiedphyA2 had been overexpressed and secreted.
      
The concentration of the phytase expressed by P.pastoris with modifiedphyA2 exceeded 15 000 U/mL, which had a 3 000-fold increase over that of originAspergillus niger 963 and was 37 times higher than that of recombinantP.
      
In this paper, progresses in researches on physiology of Zn deficiency in animals, phytate effect on bioavailability of Zn, and role of phytase in healing Zn deficiency of animals were reviewed.
      
更多          
  phytase
RecombinantPichia pastoris overexpressing bioactive phytase
      
Phytase genephyA2, whose signal peptide encoding sequence and intron sequence had been removed, was modified.
      
The result of SDS-PAGE of the phytase expressed by P.pastoris showed that the modifiedphyA2 had been overexpressed and secreted.
      
The concentration of the phytase expressed by P.pastoris with modifiedphyA2 exceeded 15 000 U/mL, which had a 3 000-fold increase over that of originAspergillus niger 963 and was 37 times higher than that of recombinantP.
      
In this paper, progresses in researches on physiology of Zn deficiency in animals, phytate effect on bioavailability of Zn, and role of phytase in healing Zn deficiency of animals were reviewed.
      
更多          
  phytase
RecombinantPichia pastoris overexpressing bioactive phytase
      
Phytase genephyA2, whose signal peptide encoding sequence and intron sequence had been removed, was modified.
      
The result of SDS-PAGE of the phytase expressed by P.pastoris showed that the modifiedphyA2 had been overexpressed and secreted.
      
The concentration of the phytase expressed by P.pastoris with modifiedphyA2 exceeded 15 000 U/mL, which had a 3 000-fold increase over that of originAspergillus niger 963 and was 37 times higher than that of recombinantP.
      
In this paper, progresses in researches on physiology of Zn deficiency in animals, phytate effect on bioavailability of Zn, and role of phytase in healing Zn deficiency of animals were reviewed.
      
更多          


A wild Penicillium strain A 2 producing phytase was mutated for obtaining the enzymatic activity as high as possible. After two cycles of the complex mutation by using HNO 2 UV and UV-5BrU as well as the routine screening procedures,6 mutants that the enzyme activity was increased 50% more than that of A 2 were obtained.Among them,the best one is A 2-U59-20 which activity is more than 0.182±0.02U/ml,as two times as that of A 2.

对产植酸酶的青霉菌株A2进行了诱变研究。分别以HNO2-UV和UV-5Bu复合处理,进行了二轮诱变筛选后,得到高于A250%以上的变异株6株,其中A2-UV59-20株的酶活达0.812±0.019u/ml,为出发菌株A2的二倍多。

The culture conditions of a strain of producing-phytase penicillium separated from the oil waste were reported.The effect of the various carbon and nitrogen sources on this strain was studied.The results indicated that the optimal carbon and nitrogen compounds for this strain were sucrose,corn starch and ammonia nitrate,peptone respectively.The best media obtained from the orthogonal test in this culture conditions are as follows:corn starch 2%,sucrose 1.5%,peptone 1%,ammonia nitrate 0.2% and a small amounts...

The culture conditions of a strain of producing-phytase penicillium separated from the oil waste were reported.The effect of the various carbon and nitrogen sources on this strain was studied.The results indicated that the optimal carbon and nitrogen compounds for this strain were sucrose,corn starch and ammonia nitrate,peptone respectively.The best media obtained from the orthogonal test in this culture conditions are as follows:corn starch 2%,sucrose 1.5%,peptone 1%,ammonia nitrate 0.2% and a small amounts of mineral salts.On the basis of the experimants,the other factors such as pH etc.were also mentioned.

从油脂废料中分离到一株产植酸酶的青霉菌株。研究了不同碳源,氮源对青霉菌液体发酵产植酸酶的影响。结果最佳碳源为蔗糖,玉米淀粉。最佳氮源为硝酸铵、蛋白胨。通过正交试验获得适于产植酸酶的液体培养基的配方为:玉米淀粉2%,蔗糖1.5%,蛋白胨1%,硝酸铵0.2%以及少许无机盐。在此基础上,对其他影响植酸酶产生的因素也作了研究。

Phytase is purified from roots of tomato plants grown under phosphorusdeficient conditions using five purification schemes. The phytase is successfully separated from the major acid phosphatase to an eletrophoretic homogeneity. The native molecular weight of this enzyme is estimated to be about 164 kD by BioGel P200 gel filtration. The molecular weight of the subunit on SDSPAGE is approximately 82 kD, indicating that the native form of the enzyme is a homodimer. The isoelectric point of tomato phytase is about...

Phytase is purified from roots of tomato plants grown under phosphorusdeficient conditions using five purification schemes. The phytase is successfully separated from the major acid phosphatase to an eletrophoretic homogeneity. The native molecular weight of this enzyme is estimated to be about 164 kD by BioGel P200 gel filtration. The molecular weight of the subunit on SDSPAGE is approximately 82 kD, indicating that the native form of the enzyme is a homodimer. The isoelectric point of tomato phytase is about 5.5. The enzyme exhibits a high affinity for phytic acid ( K m=38μmol/L), and is strongly inhibited by phosphtate, molybdate and fluoride. Among other characteristics of tomato phytase, the pH and temperature optima are 4.3 and 45℃, respectively. Tomato phytase contains a fairly high concentration of aspartic, glutamic acid and glycine residues.

运用硫铵沉淀、阴离子交换柱层析、凝胶过滤、FPLC和活性电泳等技术,从缺磷条件下生长的番茄根抽提物中分离提纯了植酸酶.该酶是分子量约为82kD的亚基组成的二聚体,等电点5.5,最适pH为4.3,对植酸钠具有很高亲和力(Km=38μmol/L).番茄植酸酶活性受无机磷酸、钼和氟化物强烈抑制.氨基酸组成分析表明,该酶谷氨酸、天冬氨酸和甘氨酸含量较高.

 
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