2. Using α-amylase to hydrolyze and establishing its optimal conditions: enzymatic content 0.5%, pH 6.2, hydrolysis temperature 98℃, hydrolysis time 3 hour, and substrate concentration 1:6.
3. The raw material ratio of barley malt: wheat malt: rice was 3:4:3. The mashing processing was decided, the adding dose of thermo-amylase, protenase and complex enzyme was 0.6mL/kg, 0.15% and 0.1%, respectively.
De-sizing, boiling and bleaching of cotton by using L40, Baisheng enzyme, hydrogen peroxide could be conducted in one step by steaming, which is the key point in this paper.
PFA is a hyperthermophilic α-amylase from Pyrococcus furious. Based on the codon bias of Pichia pastoris, the sequence of pfa gene was optimized in order that pfa could express high efficiently in the P. pastoris. Then pfa gene was inserted into expression vector pPIC9K and the recombinant plasmid pPIC9K-pfa was constructed.
A highly potent strain of Bacillus licheniformis 103 that synthesized thermostable α-amylase with temperature and pH optima of 90-95°C and 6.0-8.5, respectively, was obtained by mutagenesis and selection.
The exponential cells ofBacillus cereus immobilized in calcium alginate and spun into microcylindrical particles were used in a fluidized-bed reactor for continuous synthesis of thermostable α-amylase.
Several transformants with amylolysis were obtained and one of the fusion plasmids had an about 5.0 kb inserted DNA fragment, containing the upstream and downstream sequences of α-amylase gene fromS.