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腺癌
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    STUDY ON BINIDING CHARACTERISTICS OF ~(125)I-LABLED McAb LC-1 TO LUNG ADENOCACINOMA CELLS IN VITRO AND IN VIVO
    ~(125)Ⅰ标记单抗LC-I与肺腺癌细胞体内外结合特性的研究
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    Enzyme-linked Immunosorbent Assay for Pancreatic Cancer-associated Antigen PCA_2
    胰腺癌相关抗原PCA_2-Ag的酶联免疫分析
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    Establishment of a Model of Transplantation of Human Lung
    CEA高表达人肺腺癌裸鼠移植瘤模型的建立
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    MUTATION ANALYSIS OF p16 GENE EXON 2 IN HUMAN LUNG ADENOCARCINOMAS
    肺腺癌中 p16 基因外显子2的突变分析
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    Methods To cultivate the A549 cells in vitro, the pEGFP-N1-elafin eukaryotic expression vectors have been transfected to the cells by LipofectamineTM 2000. Elafin transfected cells incubated by the supernatant of S. epidermidis (S.epidermidis group), Pa (Pa group) and E.
    方法将pEGFP-N1-elafin转染到培养的肺腺癌细胞株A549细胞后,用表皮葡萄球菌(表葡组)、大肠埃希杆菌(E.coli组)和铜绿假单胞菌(Pa组)培养上清分别孵育细胞24h,另设转染空载体A549细胞作为正常组。
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2-Naphthylamine-free antioxidant D, containing more than 85% N-phenyl-2-naphthylamine and about 0.4% N-phenyl-1-naphthylamine, was mixed in mouse diet and fed to young male C 57/BL mice for 1.5 years. Dosage of the antioxidant was increased by steps in three experimental groups at 0.04%—0.5%, 0.08%—0.6% and 0.24%—0.8%, respectively. All surviving experimental (63) and control (19) animals were sacrificed at the end of the 2nd experimental year and subjected to detailed anatomical and histological examination....

2-Naphthylamine-free antioxidant D, containing more than 85% N-phenyl-2-naphthylamine and about 0.4% N-phenyl-1-naphthylamine, was mixed in mouse diet and fed to young male C 57/BL mice for 1.5 years. Dosage of the antioxidant was increased by steps in three experimental groups at 0.04%—0.5%, 0.08%—0.6% and 0.24%—0.8%, respectively. All surviving experimental (63) and control (19) animals were sacrificed at the end of the 2nd experimental year and subjected to detailed anatomical and histological examination. Experimental mice showed 72.7% malignant tumors and 57.1% carcinomas, including cancers of various organs with those of the lungs, salivary glands, pancreas and kidneys predominating. There was dose-response relationship regarding incidence of carcinomas. Control mice had 21% sarcomas (mainly lymphomas) but no carcinoma. Results of the present experiment confirmed earlier findings of the carcinogenicity of antioxidant D in rats and suggested selective affinity of the carcinogen to the lungs as previously suspected.

以含N-苯基-2-萘胺约85%,N-苯基-1-萘胺约4%且不含2-萘胺的国产防老剂D混于饲料中,饲喂幼年雄性C57/BL小鼠共一年半,饲料中防老剂D浓度在三个剂量组均逐步增加,分别为0.04%—0.5%、0.08%—0.6%及0.24%—0.8%。停止饲喂后半年杀死全部存活动物,作仔细的组织学检查。实验结果证明防老剂D对小鼠有致癌作用。63头实验组小鼠的恶性肿瘤总发生率为72.7%,癌瘤发病率为57.1%,其中主要包括肺癌、肝细胞癌和颌下腺癌,19头自然对照小鼠有21%肉瘤但无癌。实验和对照组癌发生率和肺、肝、颌下腺腺癌的各别发生率在统计学上均有显著差异。实验组中也较常见肝、脾、肾等血管内皮肉瘤的发生。肿瘤发生率和防老剂D的剂量之间存在着一定的相关性。

Morphology of cells of long-term cultured pulmonary squamous carcinoma cell line (LTEP-78) and cancer cells taken from sputa of fifty lung cancer patients was studied in view of clinical cytology. The results showed that the long-term cultured cell line still have had the characteristics of squamous cell carcinoma, such as round or squamous cell outline, definite cellular border, evenness in density of cytoplasm, centrally placed nuclei and so on. On the other hand, it appeared that they have undergone some...

Morphology of cells of long-term cultured pulmonary squamous carcinoma cell line (LTEP-78) and cancer cells taken from sputa of fifty lung cancer patients was studied in view of clinical cytology. The results showed that the long-term cultured cell line still have had the characteristics of squamous cell carcinoma, such as round or squamous cell outline, definite cellular border, evenness in density of cytoplasm, centrally placed nuclei and so on. On the other hand, it appeared that they have undergone some changes which resembled to adenocarcinoma cells, such as malignent signet-ring cell. We concluded, therefore, that the long-term cultured cancer cells could still keep the characteristics as cancer cells taken freshly from lung cancer patients. The differences from the fresh lung cancer cells found in cultured cells could be explained by the effects of in vitro circumstance which is quite different from that of the human body.

本文报告了从临床细胞学观点对长期体外培养的人肺癌细胞进行形态学研究的结果。发现虽然经长达一年半的体外培养,人肺鳞癌细胞系仍保留有鳞癌细胞临床诊断的标准特点。大多数细胞仍为圆形或多边形;边界清楚;核位于细胞中央;胞浆匀实;并出现典型的鳞癌细胞,如圆形癌细胞、蝌蚪形癌细胞及多核癌细胞等。在培养细胞中也出现细胞分化状态的改变,如少数细胞保存着低分化鳞癌细胞的状态,甚至呈未分化的恶性裸核,而大多数细胞转化为中分化的鳞癌细胞。值得注意的是,在少数培养细胞中出现腺癌细胞样的改变,如癌细胞的胞浆内出现单个或多个大空泡,并将核推到一边。有的细胞呈典型的恶性“印戒细胞”。因此,我们认为体外培养的人癌细胞仍可反映出人体癌细胞的临床诊断特点,所出现的差异可能是体外环境选择而使细胞改变的结果。

This work deals with a new cellline (MGc80-3) established from a pri-mary poorly differentiated mucoidadenocarcinoma of human stomach.The tumor cells were derived from thegastric cancer of a patient on March10,1980,and a new permanent cellline was established after a latent periodof 9 months.The cells grew in a mo-nolayered sheet with a population doubling time of about 25 hrs.Underphase contrast microscope,the cellswere characterized morphologically assingle nucleated epitheloid cells withprominent nucleoli...

This work deals with a new cellline (MGc80-3) established from a pri-mary poorly differentiated mucoidadenocarcinoma of human stomach.The tumor cells were derived from thegastric cancer of a patient on March10,1980,and a new permanent cellline was established after a latent periodof 9 months.The cells grew in a mo-nolayered sheet with a population doubling time of about 25 hrs.Underphase contrast microscope,the cellswere characterized morphologically assingle nucleated epitheloid cells withprominent nucleoli and rather scantycytoplasm.The nucleoli were verydistinct and irregular in shape.Allfeatures from our transmission andscanning electron microscopical obser-vations showed that the cells of MGc-80-3 cell line have the characteristicsof malignant mucoid gland cells withvigorous metabolism.The featuresare:high nuclear/cytoplasmic ratio;many prominent evaginations of wrinkled cytoplasmic membrane forming finger-like microvilli;rich cytoplasmic or-ganelles and abundant polyribosomeat secretory stage with plenty of mucoid granules located at one end of thecells.This tumor cell line can betransplanted and grown subcutaneouslyinto tumor nodules in cortisone treat-ed irradiated rats.The chromosomemake-up of MGc80-3 is hypertriploidwith a model number of 75.

从人体胃低分化粘液腺癌原发组织块培养的MGc80-3细胞系已建立成功,目前已传124代,存活23个月,并冻存21个月。单层增养细胞最短的倍增时间为25.4小时。细胞保持着胃粘液腺癌细胞的特征,并能在射线可的松处理过的大鼠皮下移植再现。该细胞系的染色体组型为超三倍体,染色体众数为75。

 
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