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液态发酵
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  “液态发酵”译为未确定词的双语例句
    Anti-oxidation activity of exopolysaccharide AbEXP-la extracted from Agaricus blazei Murrill is determined by ways of colorimetry method (·OH) and NBT light deoxidization method (O_2~-· )in vitro.
    采用比色法和NBT光还原法对姬松茸液态发酵胞外纯化多糖AbEXP-la体外抗羟基自由基(·OH)和超氧自由基(O2-·)的活性进行研究。
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  liquid-state fermentation
Two types of cultivation methods, the solid-state fermentation (SSF) and the liquid-state fermentation (LSF), were examined for V.
      
The co-culture of cellulolytic moulds and yeasts on apple pomace in solid-state fermentation (SSF) and liquid-state fermentation (LSF) increased the protein content of apple pomace.
      


Anti-oxidation activity of exopolysaccharide AbEXP-la extracted from Agaricus blazei Murrill is determined by ways of colorimetry method (·OH) and NBT light deoxidization method (O_2~-· )in vitro.The results are as follow:AbEXP-la could eliminate hydroxyl radicals and superoxide radicals.In range of 80~280 mg/L,hydroxyl radical scavenging rate of AbEXP-la reaches the peak value of 32.74% at the dose of 30 mg/L.Superoxide radical scavenging rate amounts to 69.31% at the dose of 280 mg/L.

采用比色法和NBT光还原法对姬松茸液态发酵胞外纯化多糖AbEXP-la体外抗羟基自由基(·OH)和超氧自由基(O2-·)的活性进行研究。结果表明,在30~280mg·L-1的浓度范围内,姬松茸胞外多糖AbEXP-la30mg·L-1的浓度条件下对羟基自由基的清除作用最强,清除率达32.74%;在280mg·L-1的浓度条件下对超氧自由基的清除作用最强,清除率达69.31%。

A asexuae strain producing effective component hypocrellin was isolated from the hypha of Shiraia bambusicola sporocarp. By liquid-state fermentation and orthogonal teat with shake flask culture, the optimum medium component and culture conditions were: saccharose 20 g/L, yeast extract 8 g/L, maize extract 2 g/L, pH 6.0, culture broth quantity 50/250 mL(V/V) flask, inoculum 10%(V/V), culture temperature 28℃, shake rotation 130 r/min, fermentation period 96 h. The culture can produce 8 g Hypocrellin(E~1%_1cm465=52)...

A asexuae strain producing effective component hypocrellin was isolated from the hypha of Shiraia bambusicola sporocarp. By liquid-state fermentation and orthogonal teat with shake flask culture, the optimum medium component and culture conditions were: saccharose 20 g/L, yeast extract 8 g/L, maize extract 2 g/L, pH 6.0, culture broth quantity 50/250 mL(V/V) flask, inoculum 10%(V/V), culture temperature 28℃, shake rotation 130 r/min, fermentation period 96 h. The culture can produce 8 g Hypocrellin(E~1%_1cm465=52) per liter fermentation broth. This study will provide basis for the future massively industrialized hypcorllin production.

从野生竹黄子实体分离获得能产生竹红菌素的无性株,经液态发酵培养及摇瓶正交试验获得最佳培养基配方和培养条件:蔗糖20g/L,酵母膏8g/L,玉米浆2g/L,pH6.0,摇瓶装量50/250ml(V/V),接种量10%(VV),培养温度28℃,摇床转速130r/min,培养周期96h,每升发酵液可获得色价为52的竹红菌素8g。本试验结果可为工业化生产竹红菌素提供参考。

BACKGROUND & AIM: To explore the inhibitory effect of intracellular polysaccharide(IPS) from liquid state fermentation of Phellinus igniarius on mutation induced by cyclophosphamide(CP). MATERIALS AND METHODS: The mice were divided into five groups randomly,including negative group (0.86% saline),positive group (cyclophosphamide, CP) and experiment groups of different doses of IPS(150 mg/kg,300 mg/kg,600 mg/kg).Micronucleus(MN) test of mouse bone marrow polychromatic erythrocytes(PCE) and sperm abnormalities...

BACKGROUND & AIM: To explore the inhibitory effect of intracellular polysaccharide(IPS) from liquid state fermentation of Phellinus igniarius on mutation induced by cyclophosphamide(CP). MATERIALS AND METHODS: The mice were divided into five groups randomly,including negative group (0.86% saline),positive group (cyclophosphamide, CP) and experiment groups of different doses of IPS(150 mg/kg,300 mg/kg,600 mg/kg).Micronucleus(MN) test of mouse bone marrow polychromatic erythrocytes(PCE) and sperm abnormalities test had been used,and the activity of superoxide dismutases(SOD) and the content of malondialdehyde(MDA) in serum and liver tissue of mice were measured. RESULTS: At doses of 150,300,600 mg/kg,MN inhibition rates were 24.25% ,53.36% and 63.43% , respectively.Sperm abnormality frequencies at different doses which were (60.33± 4.16)‰ ,(50.00± 4.08)‰ and (46.75± 2.98)‰ , respectively,statistically sinificant(P<0.01), compared with CP group of (94.80± 6.49)‰ .IPS of Phellinus igniarius could significantly increased the activity of SOD and decreased the content of MDA in serum and liver tissue and MN rate had significant negative correlation with the activity of SOD(rserum=- 0.998,rliver=- 0.979, P<0.05), but correlated positively with the content of MDA(rserum=0.997, rliver=0.989, P<0.05). CONCLUSION: IPS of Phellinus igniarius had significant effects on gene mutation of body cell and germ cell.The antimutagenic mechanism may lie in promoting the antioxidative functionsprotecting the biological macromolecules from the attack of free radicals and supressing the formation of lipid peroxidation products.[

背景与目的:探讨桑黄胞内多糖抗环磷酰胺致突变作用。材料与方法:选用小鼠50只,随机分为阴性对照组(0.86%生理盐水)、阳性对照组(环磷酰胺,CP)和桑黄胞内多糖不同剂量实验组(150、300、600mg/kg),采用小鼠骨髓嗜多染红细胞微核实验和小鼠精子畸形实验,研究桑黄液态发酵胞内多糖的抗环磷酰胺致突变作用,并检测小鼠血清和肝组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:150、300、600mg/kg剂量的桑黄胞内多糖的微核抑制率分别为24.25%、53.36%和63.43%;各剂量组精子畸形率分别为(60.33±4.16)‰、(50.00±4.08)‰、(46.75±2.98)‰,CP组为(94.80±6.49)‰,各剂量组与CP组间的差异具有统计学意义(P<0.01);桑黄胞内多糖能明显增强小鼠SOD活性,降低MDA含量;小鼠骨髓嗜多染红细胞微核率与SOD活性呈明显负相关(r血清=-0.998,r肝=-0.979,P均<0.05),与MDA含量呈明显正相关(r血清=0.997,r肝=0.989,P均<0.05)。结论:桑黄胞内多糖对由环磷酰胺引起的体细胞和生殖细胞的基因...

背景与目的:探讨桑黄胞内多糖抗环磷酰胺致突变作用。材料与方法:选用小鼠50只,随机分为阴性对照组(0.86%生理盐水)、阳性对照组(环磷酰胺,CP)和桑黄胞内多糖不同剂量实验组(150、300、600mg/kg),采用小鼠骨髓嗜多染红细胞微核实验和小鼠精子畸形实验,研究桑黄液态发酵胞内多糖的抗环磷酰胺致突变作用,并检测小鼠血清和肝组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:150、300、600mg/kg剂量的桑黄胞内多糖的微核抑制率分别为24.25%、53.36%和63.43%;各剂量组精子畸形率分别为(60.33±4.16)‰、(50.00±4.08)‰、(46.75±2.98)‰,CP组为(94.80±6.49)‰,各剂量组与CP组间的差异具有统计学意义(P<0.01);桑黄胞内多糖能明显增强小鼠SOD活性,降低MDA含量;小鼠骨髓嗜多染红细胞微核率与SOD活性呈明显负相关(r血清=-0.998,r肝=-0.979,P均<0.05),与MDA含量呈明显正相关(r血清=0.997,r肝=0.989,P均<0.05)。结论:桑黄胞内多糖对由环磷酰胺引起的体细胞和生殖细胞的基因突变有着明显的拮抗作用。其机制可能与通过提高机体抗氧化防御系统的功能,防止有害自由基对细胞内生物大分子的损伤,抑制脂质过氧化的产物有关。

 
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