The best media for different cultural stages are bud induction MS+6-BA 2 mg/L+NAA 0.2 mg/L, Differentiation bud MS+6-BA 1 mg/L+NAA 0.1 mg/L, Strengthening seedling MS+0.1 mg/L NAA, Rooting 1/2MS+IBA 0.2 mg/L.
The results showed that the appropriate medium for bud induction is MB+6-BA 1.0 mg/L+NAA 0.2 mg/L, the medium of multiplication is (MB+)6-BA 0.5 mg/L+NAA 0.1 mg/L, and the medium of rooting is 1/2 MS+NAA 0.3 mg/L.
This paper studied the rapid propagation method of Opuntia vilpa alta Haw in plant tissue culture,We got the results: explants of differentiation rate to 77.3% with medium MS 6-BA 3.0mg/L&NAA 0.1 mg/L in bud induction;
Immature leaflet explants were found to be more responsive to shoot induction than mature leaflet explants.
Co-segregation of the NR- phenotype and the altered response to shoot induction on standard medium suggest the involvement of the nitrate-assimilatory pathway in determining shoot regeneration ability.
minima, shoot induction was obtained only after transformation by bacteria carrying pGV 2215.
In response to shoot induction, about 10% of the root lines produced shoots through callus formation.
Incubation in semi-solid media at 15°C with continuous illumination followed by growth in agitated liquid media was essential for shoot induction and development in primary explants of E.